LOCUS       CP099164                2460 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB28-E3-C03 plasmid unnamed5, complete
            sequence.
ACCESSION   CP099164
VERSION     CP099164.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488598
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB28-E3-C03
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 105x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:31:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,712
            CDSs (total)                      :: 4,591
            Genes (coding)                    :: 4,369
            CDSs (with protein)               :: 4,369
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 61 of 222
            Pseudo Genes (incomplete)         :: 170 of 222
            Pseudo Genes (internal stop)      :: 40 of 222
            Pseudo Genes (multiple problems)  :: 40 of 222
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB28-E3-C03"
                     /isolation_source="Pooled sediment sample collected from
                     floor of pig farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(1895..2460,1..451))
                     /locus_tag="NFK34_23550"
     CDS             complement(join(1895..2460,1..451))
                     /locus_tag="NFK34_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WFY47095.1"
                     /translation="MRSPDWLDVRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEAT
                     PRQQAEFFARSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMREDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQKNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="plasmid recombination protein"
     gene            1097..1579
                     /locus_tag="NFK34_23555"
     CDS             1097..1579
                     /locus_tag="NFK34_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFY47096.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     Typing          complement(3..451)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.667"
                     /coverage="99.0"
                     /evalue="2.79e-99"
     Typing          complement(1899..2458)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.722"
                     /coverage="99.0"
                     /evalue="4.1e-100"
ORIGIN
        1 gcgtcaatgg caccacgaac gcagacaggt gcggggtggc ctcgtcccgg tgaaccaccg
       61 ccgccacaac acgatccttc ccgtatttct tctccagcca ttgctcagag cgggcgaaaa
      121 actccgcctg ctgccgcggt gtggcctcgt tccaccactc agggcttgcc gtcataacgt
      181 attcaacagc cagtaccgca tctttacggc gtttttccgg caacagctcc cgcagctttc
      241 ccatcgcctc atctgccgac tgagacacag agcagtagtt ttctggtgtc cgttcagcgt
      301 cagcattcgg cgtctcacgc tccctgtagc agtgctgcaa agcgctggcc acactgccca
      361 ttcccgtcag ctttttgcag cgcattatcg cgtaattcgc catgcttacc ccctcgttct
      421 ggcgtacatc cagccagtcg ggggatcgca ttgcgatgcc tccggcgggc cactatcagg
      481 atgatagtgt actcactaga ctttgctacg caaaatcgtt cgggcagcaa gctgccagaa
      541 ggtcaaaatc aacggcagtc tggcaggtgg aaggcgtcgc tccgcgccac cccccaccac
      601 tgccaagcct gttttatccg gtgagtcgtc aagggtaaat ggcactcgct tcgctcgccc
      661 ctgacgaaat caggcaatga atatcgtttt tgaaagagca ggaaccgctc gccgcaagaa
      721 cggattaggg agcggcatcg ccgcgagtct gtccgttcga ggaagcggaa tttccactcc
      781 ccggacatag aaatccgtac gctccagact caaaaaagct cgtttttcga tgtttaaaaa
      841 tggcgttttt cagcctttat tttccctctt aatatataga tatagatatg ggaacccgct
      901 tgtaccaagg gctagagcga agaatcgtac tcacctcaag gactaatcgt actcacctca
      961 agtactaatc gtactcacct caaggactaa tcgtactcac ctcaagtact aatcgtactt
     1021 gatgcaaagg acttgactgt atgtctcttt agtacttaaa tgcgagtact aaatcatggc
     1081 gaaacgtggg gctggagtga atacgaaaaa gatttctagg cttgaacggc atacatacat
     1141 ggatgaaaac ggtgaggtaa aacaagactc taagatcgag accttttcca tcccagccga
     1201 acctccctac gtcaagctat acattgcaga cattgcgatg attcatggtt tatcaaaatc
     1261 tcaaaatgat gtgcttttac aattggcaat gctgattgga tgggatgggt ttgtgtctgt
     1321 atcaaaaact agatttgaaa aaaccataca gcccagggtt catattgaat atcagacctt
     1381 taaaaacata attgcggctc ttgtagataa acaaatattc ttccgggcag ggcgtgggga
     1441 acttgaagct aacccctttc tttttgcacg gggagactgg ccatcaattt acgaaagaag
     1501 aaagaaaatt gagttaaaga tcagttatga caccaatgga agaacggtgc agacgaaggt
     1561 aatggatgat gaatgctgaa gataaacttt ttaaaaatgt agacaggcta atgctgcatg
     1621 attcactaaa aaagaatgag gttctgactg tttgggagcg tactttttta agcagtgttt
     1681 acagcatata tcgtagaact agcggtaata aatactcaac taagggtttg tcaccaaagc
     1741 agaaaaacgc agctttttct attttgaaaa aatacaatta ccatctgatg gattagctgg
     1801 cctcagcgtt atacagggcg gcagaacctg atctctggaa tgccgcaaat gacgtcactt
     1861 tgcggcgtta gccctatcct gcatcgcatc aaaactaacg ctccatacca cccatgccac
     1921 gcgatcgcgt ctgtcgctgt ttttcacgtt caatacgctg ctgtttttcc ttttcctggt
     1981 ttttctgctt aaaccgctga gccatcatca ggatttcggt catctgctct ctggaaagcc
     2041 ccttaaaagg ctctgtaacg ctctgtaggc gtttctgctg cgcatccata gtcttctgta
     2101 gctcacgcgc tctttttgcg ttctgggcgc tctgagaggc catagcgatg gttcctgcaa
     2161 agccatcgtt cactgcgtgg cttagacgtt tggctatggc tgcctgatct tccacgtctt
     2221 tggtgaaaat gcccttccgc aatacacgcg gctccagcgc ctccggcgag atcgatacct
     2281 ggctacgggt gccacggttt atcgattcgt agtagtgctg aactgtctga tgcgtggcgc
     2341 ggcttccctc gatgccgcgc tcaagaccca gtttttttac tgattcagcg taggtgctct
     2401 gatcttcacg catctttgac cgcccgccaa taaactcttt cgcggacagc cgtccatcct
//
