LOCUS       CP099155                7941 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB28-E3-C06 plasmid unnamed3, complete
            sequence.
ACCESSION   CP099155
VERSION     CP099155.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488599
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7941)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB28-E3-C06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 112x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:26:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,813
            CDSs (total)                      :: 4,694
            Genes (coding)                    :: 4,500
            CDSs (with protein)               :: 4,500
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 74 of 194
            Pseudo Genes (incomplete)         :: 120 of 194
            Pseudo Genes (internal stop)      :: 41 of 194
            Pseudo Genes (multiple problems)  :: 33 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB28-E3-C06"
                     /isolation_source="Pooled sediment sample collected from
                     floor of pig farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(7029..7941,1..653))
                     /locus_tag="NFK35_23955"
     CDS             complement(join(7029..7941,1..653))
                     /locus_tag="NFK35_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0016021 - integral component of membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WFY51581.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            913..1350
                     /locus_tag="NFK35_23960"
     CDS             913..1350
                     /locus_tag="NFK35_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WFY51582.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="entry exclusion protein 1"
     gene            1431..1850
                     /locus_tag="NFK35_23965"
     CDS             1431..1850
                     /locus_tag="NFK35_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WFY51583.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2413..2646)
                     /locus_tag="NFK35_23970"
     CDS             complement(2413..2646)
                     /locus_tag="NFK35_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WFY51584.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2653..3171)
                     /locus_tag="NFK35_23975"
     CDS             complement(2653..3171)
                     /locus_tag="NFK35_23975"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WFY51585.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSC
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            complement(3209..3628)
                     /locus_tag="NFK35_23980"
                     /pseudo=""
     CDS             complement(3209..3628)
                     /locus_tag="NFK35_23980"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3837..4160)
                     /locus_tag="NFK35_23985"
     CDS             complement(3837..4160)
                     /locus_tag="NFK35_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WFY51586.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4225..4416
                     /locus_tag="NFK35_23990"
     CDS             4225..4416
                     /locus_tag="NFK35_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WFY51578.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5511..6479
                     /locus_tag="NFK35_23995"
     CDS             5511..6479
                     /locus_tag="NFK35_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WFY51579.1"
                     /translation="MKDQITHPPDNTDHSVAKQKFRITNWSTYNKALINRGSLTFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVPLR
                     CPDYTSVSKRAKSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRIWRK
                     LHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRLSGSNARWKWTTEYNRRSIAETAMYRMK
                     QLLGDSLTLRDYDGQVAEAMAMVRALNRMTKAGMPESVRIA"
                     /name="IS5 family transposase"
     gene            complement(6524..6643)
                     /locus_tag="NFK35_24000"
     CDS             complement(6524..6643)
                     /locus_tag="NFK35_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WFY51580.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSWLC"
                     /name="colicin release lysis protein"
     gene            6805..7032
                     /locus_tag="NFK35_24005"
     CDS             6805..7032
                     /locus_tag="NFK35_24005"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WFY51587.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          complement(4502..4589)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          4600..5158
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.606"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2310..3847)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.267"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaacgggtat tgagcgtatt aatctcttca tccactttcg ccagctcaga ttgtgcagca
       61 gcaagatttt tttgtgccac ctccacagcc cgggcctctt cactcaatgc tgccaggcgt
      121 ttctcttcat cctcagccag tttcaattgg cgttctgttt cagcctgctc cttctctatc
      181 tctttacgtc gttgttctgc ttcctgaaaa gccttttccg cagcttccgc ttctttacgg
      241 gctttttctt ctgcttttgc aaggcgcaac cgctctgctt ctgcctgcat cgctgcatta
      301 ttggcatgag ccaggtcaat aacctccgga tgagtggaat tatggcgaag cgcctcattc
      361 acaatatcct tcagatgttg agtcagcgca tcccggtttg cttttgcttt agcctgtgct
      421 tctgcggcag cttttgctcg ggcagcctgt tctgcctgcg ttttcttcaa ttgagcagta
      481 gaccatttag ctgtggcatg aatggctgca gaactttcac ttttgctacc tccagttcca
      541 ccaccaccgc cagagccact cccgtctgga ttaccattca aaagagtaat gattacctgc
      601 cccttatcat cataaggaac accatcttta tagtacgcta cagctgtttc cattataaaa
      661 tcctctttga cattaaaaac aaatagttaa aagcaatata ctgtatatat aatcactggt
      721 tttatataca gtttaaaatt acgccgctgc attttccctg tcaaccctgt ggattttcat
      781 ttttgtgaaa acgatcaaaa aaacagccac ccacaccacg tgctatttcc acccgatgcc
      841 acaaaaacca gcacaaacat taccgttctc agacctcatt atgttttact gaaaccatga
      901 gatgagacac ctatgggaca ctgtaacttt atggcatggc acacactccg ggacgcactg
      961 aaaatgactg gaagatcgcg ttcacagttt taccgtgata tgcgctcagg tcttgtcagt
     1021 taccgtacca gccaggacgg acgacgggag tttgaaacca gtgaactgat ccgggcatac
     1081 ggcgaattaa agcggaatga gacaccagaa aggcacactc aaggacactc agaaaaacct
     1141 catgatcagc agacagaacg cattctccgg gaactgaatg agctgaaaca atgcctgacg
     1201 ctgatgcttg aggataaaca agcccaggat acggagcgcc ggcgccagga agcagaacgg
     1261 aaacagctac agaatgagat agcccagctc aggcaggctc tggagcagga aaagaaacgg
     1321 ggattctggt ccaggctgtt cggtcactga acgcgcccag aaactgatga taagataatc
     1381 ttcggataat aactcaccga gaataaatac tttaaggtag ggagacactc atgagacgta
     1441 ccggaaacaa actttgtctt atcgccatga taacagcaac agtagctctc acagcctgta
     1501 ccccaaaggg cagcgtggaa caacataccc ggcattacgt atatgcttct gatgacggtt
     1561 ttgatcccaa cttttccacc caaaaagccg acacaacacg aatgatggtg cctttttttc
     1621 ggcagttctg ggatatggga gctaaagata aagcgacagg aaaatcacgg agtgatgtgc
     1681 aacaacgcat tcaacagttt cacagccagg aatttttaaa ctcactccgg ggcacaactc
     1741 aatttgcggg tactgattac cgcagcaaag accttacccc gaaaaaatcc aggctgctgg
     1801 ctgacacgat ttctgcggtt tatctcgatg gctacgaggg cagacagtaa gtggatttac
     1861 cataatccct taattgtacg caccgccgaa acgcgttcgg tgcgatcacg gcagcagaca
     1921 ggtaaaaatg gcaacaaacc acccggaaaa ctgccgcgat cgcacccggt aaattttaac
     1981 cacatgcata cctatgcagc caggtgaatc acgcaggaat tgccagtcag agaccgctga
     2041 aacggatttt tttcatactt tcggaagcga agaagacggg gatttgagca ggaaaacaga
     2101 agttcagggg aatgagcgcg atctggcaat agaggcaaaa acacgactat gaaagacaca
     2161 ccagaatcgc gcccgtatgc gttttaacgc gttcctgtgt tttcagcaag gtttatgact
     2221 gaagcgcgtt cccggtgccc cgaaccaacc attatcccaa aaaatgcacc ggaaacgcgc
     2281 tgacaacatt ttagcctgtc tctgattacc atcccagcga agggccatcc agtgtccgtt
     2341 cctgtatttc cagctggcgc tcacgctcca gggataactc atgttcgcgt tcctgtatca
     2401 gccgttcgtc ccttatctcc tgttctcgct gtataactgg ctcaagcgtt ctgtccgctc
     2461 gctcaagtgc tgcacctgct gactcaactt catgacccgc tcgttcagca tcgcgttgtc
     2521 ccgttgcgta agcgaaaaca ttttctgcaa ttccacgaag gcgctctccc attcgttcag
     2581 ccgctgcata tagtcctgtt gcagttgctc taatgcgttc agcaaatgtc tttccagctc
     2641 cgtcactctg tgtcactcca tcagttgtac ccagtccttc ccctgatggg gaatcacctc
     2701 tgctgatttc ccgtacggaa ggaccagaaa tttcctgttc ccgtcctgta cgaactccac
     2761 gccccatgtc ttcgcgttca gtttctgcaa tgtctcttcc tgcttcctga tatcttccag
     2821 gttcgcctgt atcatccctc caagatacca gagcgtcccg caactcacgg taaacaggaa
     2881 aatgaccatc cccagtaaca tcatgcccgt attccctgcc agctttaaca cgtccttcct
     2941 gtgctgcatc atcgcctctt tcaccccttc ccggtgcttt ttcagtgatt cctctgtcga
     3001 agctgtgaac agggctatag cgtctttgat tttcgtctcg tttgatgtca cagccttgct
     3061 tacagatttt tcgagcttgc tgaactcgtc gttcagcatt gtctctgtag attcggctct
     3121 ctctttcagc tttttctcga actccgcgcc cgtctgtaaa agattgctca tagaatgctc
     3181 ctttcagtct gatattcttc ccgccgttcg ggtctgcaat gctgatactg cttcgcgtca
     3241 ccctgaccac ttcaatccct gcttccgtga gcgcctgaat cacatcctga cggcctttta
     3301 tctctcctgc atggtaaaga cgtgtgacgc cctcagcaaa cgcctgtttc gtttccggca
     3361 ggttatcagg gagagtcagt atccggcggt tctccggggc gttggggtca tgcagcccgt
     3421 aatggtgatt caccagtgtc tgccaggcat caattctcgg cctgtctgcc cggtcgtagt
     3481 acggctggag gcgttttccg gtctgtagct ccatattcgg aatgacaaaa ttcagctcaa
     3541 gccgtccctt gtcctggtgc tccacccaca ggatgctgta ctgatttttt tcgagaccgg
     3601 gcatcagtac acgctcaaag ctcgccatca ctttttcacg ccctcccggc ggcagctcct
     3661 tctccgcgaa cgacagaaca ccggacgtgt atttcttcgc aaatggcgtg gcatcgatga
     3721 gttcccggac ttcttccgga ttaccctgaa gcaccgttgc cccttcccgg ttacgctccc
     3781 ttcccagcag gtaatcaacc ggaccactgc caccaccttt tcccctggca tgaaacttaa
     3841 ctatcatccc gcgccccctg ttccctgacg gccagacgca accggcgcag ctcatccccg
     3901 atggccatca gtgcggccac cacctgaacc cggtcgccgg aagaccactg tccgctgttc
     3961 accttacggg ctgtctggtt caggttattc ccgatggctg ccagctgacg cagtaacggc
     4021 ggtgccagtg tcggcagcct cccggaacgg gcaaccggct cacccaggca gacccgccgc
     4081 atccataccg ccagttgttt accctcacag cgttccagta accgggcatg ttcatcatca
     4141 gtaacccgta tcgtgagcat cctctcgcgt ttcatcggta tcattacccc catgaacaga
     4201 aatccccctt acacggaggc atcagtgact aaacaggaaa aaaccgccct taacatggcc
     4261 cgctttatca gaagccagac attaacgctg ctggagaagc tcaacgaact ggacgcggat
     4321 gaacaggcag atatctgtga atcgcttcac gaccacgccg atgagcttta ccgcagctgc
     4381 ctcgcgcgct tcggggatga cagtgaaaac ctctgacata tgcagctccc ggagacggtc
     4441 acagcttgcc tgtgagcgga tgccgggagc agacaagccc gccagggcgc gtcagggtgt
     4501 tttagcgggt ttcggggcgc agccctgaac cagtcacgta gcgatagcgg ggtgtatact
     4561 ggcttaacta tgcagcatca gtgcggattg tatgaaaagt gcaccatgac cagtgtgaaa
     4621 taccgcacag atgcgtaagg agaaaatgca cgttccggcg cttttccgct tcctcgctca
     4681 ctgactcgct acgctcggtc gttcggctgc ggcgagcggt actgactcac tcaaaaacga
     4741 taatacagtt atccacagaa tccggggata agaccggaaa gaacatgtgc gcaaaaaggc
     4801 aaagcccaac aaaaggccac gccgatggcg tttttccata ggctccgccc ccctgacgag
     4861 catcacaaaa accgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4921 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4981 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5041 tggtatctcg gctcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5101 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5161 cacgacttag cgccactggc agcagccgtt ggtaacttgg ttagcagagc gatgatggcg
     5221 caaatggtgc aacagagttc ttgaagtagt ggcccgacta cggctacact ggaaggacag
     5281 tatttggtat ctgcgctctg ctgaagccag ttacctcggt taagcagttc cccaactgac
     5341 ttaaccttcg atcaaaccgc ctccccaggc ggttttttcg tttacaggca gcagattacg
     5401 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaaca gagatccccg
     5461 tcaatttaga agaagatggg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc
     5521 agatcacgca tcctcccgac aacacagacc attccgtggc aaagcaaaag ttcagaatca
     5581 ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg
     5641 atgatgaggc cattcaggcc tggtatgagt cggcaacgcc ttcatcacga ggaaggcccc
     5701 agcgctattc tgatctcgcc atcaccaccg ttctggtgat taaacgcgta ttccggctga
     5761 ccctgcgggc tgcgcagggt tttattgatt ccatttttgc cctgatgaac gttccgttgc
     5821 gctgcccgga ttacaccagt gtcagtaagc gggcaaagtc ggttaatgtc agtttcaaaa
     5881 cgtccacccg gggtgaaatc gcacacctgg tgattgattc caccgggctg aaggtctttg
     5941 gtgaaggcga atggaaagtc agaaagcacg gcaaagagcg ccgtcgtatc tggcgaaagt
     6001 tgcatcttgc tgttgacagc aacacacatg aagttgtctg tgcagacctg tcgctgaata
     6061 acgtcacgga ctcagaagcc ttcccgggcc ttatccggca gactcacaga aaaatcaggg
     6121 cagccgcggc agacggggct tacgataccc ggctctgtca cgatgaactg cgccgcaaaa
     6181 aaatcagcgc gcttattcct ccccgaaaag gagcaggtta ctggcccggt gagtacgcag
     6241 accgcaaccg tgccgttgct aatcagcggc tgagcggaag caatgcacgg tggaaatgga
     6301 caacggaata taaccgtcgc tcgatagcgg aaacggcaat gtacagaatg aagcagttgt
     6361 tgggagattc actgacgctg cgtgactacg atggtcaggt agcggaagct atggccatgg
     6421 tgcgtgcgtt gaacaggatg acaaaggctg ggatgccaga aagcgtgcgt attgcctgaa
     6481 aatccagcca gctacagggt cgttcgcacg aaatcttatt tattcaacaa agccaagaag
     6541 atgatggagc cacagtccct ccctgaacat cacgtatata gttagcctga catccaacaa
     6601 ggaggtttat agcgaatatt cccacaaaaa atcttttcct cataactcga tccttataaa
     6661 atgaaaagaa tatataacga ggtttaattt atgagtttaa gatactacat aaaaaatatt
     6721 ttgtttggcc tatactgcgc acttatatat atatacctta taacaaaaaa caacgaaggg
     6781 tattatttcc tagcgtcaga taagatgcta tacgcaatag tgataagcac tattctatgc
     6841 ccatattcaa aatatgctat tgaacacata ttttttaagt tcataaagaa agattttttc
     6901 agaaaaagaa aaaacctaaa taatgccccc gtggcaaaat taaacctatt tatgctatac
     6961 aatctacttt gtttggtcct agcaatccca tttggattgc taggactgtt tatatcaata
     7021 aagaataatt aaatacccaa cacctcattt atagtattaa gtttattctt atcaataaag
     7081 gcacatagaa tgcctgtaac aatagcaatc ccccagatac ctaatgtagt tccagcaagc
     7141 acactaaaaa gtaaaacaac aacataacta actccagcat ctacagcttt cttctctaat
     7201 gtaagaaata gcggcttcca gtcacctgta tccttaattt ttaggatatc agataccaca
     7261 tcatatccaa aagaaacatg tcccgtaatc ttcaagtact tggcaaactg atctaaatgc
     7321 ttagcccagt cttcatactt aaccgcctcc agtgcattga aaattgcatc acggtctgct
     7381 ttgctgaatt tcttatttaa tacatccttg tatttctcaa aagcagctag agcttcattc
     7441 acattgctga ttttcttacc tttagattta tcagcaagtt cctgtgccat ttttgaatat
     7501 ttttcaccat atttttcaga tagcgtttga taaaagctaa ctgttgcatc cacagcatcc
     7561 ttaatctgcg agttcaggag attagtctgt gctgttttca aattatcttc agcatcatga
     7621 atacgtgaaa ctgctgtact tcgcttatta ttagcctgag aaatagcctc ttggattcca
     7681 tttatctcag agctaatctg gttaagacgc gtttctgatg ctgttacctg cttttgtttt
     7741 tcctccatct catcaccagc tctcgcccgt agtctggtgg cctcaaaaaa aggacgactc
     7801 tgaagcggat cattcgccct aggagataaa agtttaaccc tttcatccag ttctttatat
     7861 ttagcagatg cctgatccaa ctcatttcgt tttccggaca gcgtattcgt ttctgcatca
     7921 cgggcatgaa tactggagct t
//
