LOCUS       CP099151                2460 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB28-SO-C01 plasmid unnamed4, complete
            sequence.
ACCESSION   CP099151
VERSION     CP099151.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488600
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB28-SO-C01
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 134x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:22:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,470
            CDSs (total)                      :: 4,351
            Genes (coding)                    :: 4,109
            CDSs (with protein)               :: 4,109
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 69 of 242
            Pseudo Genes (incomplete)         :: 162 of 242
            Pseudo Genes (internal stop)      :: 50 of 242
            Pseudo Genes (multiple problems)  :: 37 of 242
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB28-SO-C01"
                     /isolation_source="Pooled soil sample collected from floor
                     of pig farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(2070..2460,1..50)
                     /locus_tag="NFK36_22340"
     CDS             join(2070..2460,1..50)
                     /locus_tag="NFK36_22340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFY59027.1"
                     /translation="MDENGEVKQDSKIETFSIPAEPPYVKLYIADIAMIHGLSKSQNDV
                     LLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVDKQIFFRAGRGELEA
                     NPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     gene            37..267
                     /locus_tag="NFK36_22345"
     CDS             37..267
                     /locus_tag="NFK36_22345"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFY59028.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKYNYHLMD"
                     /name="hypothetical protein"
     gene            complement(366..1382)
                     /locus_tag="NFK36_22350"
     CDS             complement(366..1382)
                     /locus_tag="NFK36_22350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WFY59029.1"
                     /translation="MRSPDWLDVRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEAT
                     PRQQAEFFARSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMREDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQKNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="plasmid recombination protein"
     Typing          complement(370..1382)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.041"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acaccaatgg aagaacggtg cagacgaagg taatggatga tgaatgctga agataaactt
       61 tttaaaaatg tagacaggct aatgctgcat gattcactaa aaaagaatga ggttctgact
      121 gtttgggagc gtactttttt aagcagtgtt tacagcatat atcgtagaac tagcggtaat
      181 aaatactcaa ctaagggttt gtcaccaaag cagaaaaacg cagctttttc tattttgaaa
      241 aaatacaatt accatctgat ggattagctg gcctcagcgt tatacagggc ggcagaacct
      301 gatctctgga atgccgcaaa tgacgtcact ttgcggcgtt agccctatcc tgcatcgcat
      361 caaaactaac gctccatacc acccatgcca cgcgatcgcg tctgtcgctg tttttcacgt
      421 tcaatacgct gctgtttttc cttttcctgg tttttctgct taaaccgctg agccatcatc
      481 aggatttcgg tcatctgctc tctggaaagc cccttaaaag gctctgtaac gctctgtagg
      541 cgtttctgct gcgcatccat agtcttctgt agctcacgcg ctctttttgc gttctgggcg
      601 ctctgagagg ccatagcgat ggttcctgca aagccatcgt tcactgcgtg gcttagacgt
      661 ttggctatgg ctgcctgatc ttccacgtct ttggtgaaaa tgcccttccg caatacacgc
      721 ggctccagcg cctccggcga gatcgatacc tggctacggg tgccacggtt tatcgattcg
      781 tagtagtgct gaactgtctg atgcgtggcg cggcttccct cgatgccgcg ctcaagaccc
      841 agttttttta ctgattcagc gtaggtgctc tgatcttcac gcatctttga ccgcccgcca
      901 ataaactctt tcgcggacag ccgtccatcc tgcgtcaatg gcaccacgaa cgcagacagg
      961 tgcggggtgg cctcgtcccg gtgaaccacc gccgccacaa cacgatcctt cccgtatttc
     1021 ttctccagcc attgctcaga gcgggcgaaa aactccgcct gctgccgcgg tgtggcctcg
     1081 ttccaccact cagggcttgc cgtcataacg tattcaacag ccagtaccgc atctttacgg
     1141 cgtttttccg gcaacagctc ccgcagcttt cccatcgcct catctgccga ctgagacaca
     1201 gagcagtagt tttctggtgt ccgttcagcg tcagcattcg gcgtctcacg ctccctgtag
     1261 cagtgctgca aagcgctggc cacactgccc attcccgtca gctttttgca gcgcattatc
     1321 gcgtaattcg ccatgcttac cccctcgttc tggcgtacat ccagccagtc gggggatcgc
     1381 attgcgatgc ctccggcggg ccactatcag gatgatagtg tactcactag actttgctac
     1441 gcaaaatcgt tcgggcagca agctgccaga aggtcaaaat caacggcagt ctggcaggtg
     1501 gaaggcgtcg ctccgcgcca ccccccacca ctgccaagcc tgttttatcc ggtgagtcgt
     1561 caagggtaaa tggcactcgc ttcgctcgcc cctgacgaaa tcaggcaatg aatatcgttt
     1621 ttgaaagagc aggaaccgct cgccgcaaga acggattagg gagcggcatc gccgcgagtc
     1681 tgtccgttcg aggaagcgga atttccactc cccggacata gaaatccgta cgctccagac
     1741 tcaaaaaagc tcgtttttcg atgtttaaaa atggcgtttt tcagccttta ttttccctct
     1801 taatatatag atatagatat gggaacccgc ttgtaccaag ggctagagcg aagaatcgta
     1861 ctcacctcaa ggactaatcg tactcacctc aagtactaat cgtactcacc tcaaggacta
     1921 atcgtactca cctcaagtac taatcgtact tgatgcaaag gacttgactg tatgtctctt
     1981 tagtacttaa atgcgagtac taaatcatgg cgaaacgtgg ggctggagtg aatacgaaaa
     2041 agatttctag gcttgaacgg catacataca tggatgaaaa cggtgaggta aaacaagact
     2101 ctaagatcga gaccttttcc atcccagccg aacctcccta cgtcaagcta tacattgcag
     2161 acattgcgat gattcatggt ttatcaaaat ctcaaaatga tgtgctttta caattggcaa
     2221 tgctgattgg atgggatggg tttgtgtctg tatcaaaaac tagatttgaa aaaaccatac
     2281 agcccagggt tcatattgaa tatcagacct ttaaaaacat aattgcggct cttgtagata
     2341 aacaaatatt cttccgggca gggcgtgggg aacttgaagc taaccccttt ctttttgcac
     2401 ggggagactg gccatcaatt tacgaaagaa gaaagaaaat tgagttaaag atcagttatg
//
