LOCUS       CP099146                2460 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB28-SO-C04 plasmid unnamed4, complete
            sequence.
ACCESSION   CP099146
VERSION     CP099146.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488601
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB28-SO-C04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 143x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 19:00:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,348
            Genes (coding)                    :: 4,105
            CDSs (with protein)               :: 4,105
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 70 of 243
            Pseudo Genes (incomplete)         :: 162 of 243
            Pseudo Genes (internal stop)      :: 50 of 243
            Pseudo Genes (multiple problems)  :: 37 of 243
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB28-SO-C04"
                     /isolation_source="Pooled soil sample collected from floor
                     of pig farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2324..2460,1..346))
                     /locus_tag="NFK37_22325"
     CDS             complement(join(2324..2460,1..346))
                     /locus_tag="NFK37_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFY63183.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     gene            992..2008
                     /locus_tag="NFK37_22330"
     CDS             992..2008
                     /locus_tag="NFK37_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WFY63184.1"
                     /translation="MRSPDWLDVRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEAT
                     PRQQAEFFARSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMREDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQKNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="plasmid recombination protein"
     gene            complement(2107..2337)
                     /locus_tag="NFK37_22335"
     CDS             complement(2107..2337)
                     /locus_tag="NFK37_22335"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFY63182.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKYNYHLMD"
                     /name="hypothetical protein"
     Typing          993..2005
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.041"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttccccacg ccctgcccgg aagaatattt gtttatctac aagagccgca attatgtttt
       61 taaaggtctg atattcaata tgaaccctgg gctgtatggt tttttcaaat ctagtttttg
      121 atacagacac aaacccatcc catccaatca gcattgccaa ttgtaaaagc acatcatttt
      181 gagattttga taaaccatga atcatcgcaa tgtctgcaat gtatagcttg acgtagggag
      241 gttcggctgg gatggaaaag gtctcgatct tagagtcttg ttttacctca ccgttttcat
      301 ccatgtatgt atgccgttca agcctagaaa tctttttcgt attcactcca gccccacgtt
      361 tcgccatgat ttagtactcg catttaagta ctaaagagac atacagtcaa gtcctttgca
      421 tcaagtacga ttagtacttg aggtgagtac gattagtcct tgaggtgagt acgattagta
      481 cttgaggtga gtacgattag tccttgaggt gagtacgatt cttcgctcta gcccttggta
      541 caagcgggtt cccatatcta tatctatata ttaagaggga aaataaaggc tgaaaaacgc
      601 catttttaaa catcgaaaaa cgagcttttt tgagtctgga gcgtacggat ttctatgtcc
      661 ggggagtgga aattccgctt cctcgaacgg acagactcgc ggcgatgccg ctccctaatc
      721 cgttcttgcg gcgagcggtt cctgctcttt caaaaacgat attcattgcc tgatttcgtc
      781 aggggcgagc gaagcgagtg ccatttaccc ttgacgactc accggataaa acaggcttgg
      841 cagtggtggg gggtggcgcg gagcgacgcc ttccacctgc cagactgccg ttgattttga
      901 ccttctggca gcttgctgcc cgaacgattt tgcgtagcaa agtctagtga gtacactatc
      961 atcctgatag tggcccgccg gaggcatcgc aatgcgatcc cccgactggc tggatgtacg
     1021 ccagaacgag ggggtaagca tggcgaatta cgcgataatg cgctgcaaaa agctgacggg
     1081 aatgggcagt gtggccagcg ctttgcagca ctgctacagg gagcgtgaga cgccgaatgc
     1141 tgacgctgaa cggacaccag aaaactactg ctctgtgtct cagtcggcag atgaggcgat
     1201 gggaaagctg cgggagctgt tgccggaaaa acgccgtaaa gatgcggtac tggctgttga
     1261 atacgttatg acggcaagcc ctgagtggtg gaacgaggcc acaccgcggc agcaggcgga
     1321 gtttttcgcc cgctctgagc aatggctgga gaagaaatac gggaaggatc gtgttgtggc
     1381 ggcggtggtt caccgggacg aggccacccc gcacctgtct gcgttcgtgg tgccattgac
     1441 gcaggatgga cggctgtccg cgaaagagtt tattggcggg cggtcaaaga tgcgtgaaga
     1501 tcagagcacc tacgctgaat cagtaaaaaa actgggtctt gagcgcggca tcgagggaag
     1561 ccgcgccacg catcagacag ttcagcacta ctacgaatcg ataaaccgtg gcacccgtag
     1621 ccaggtatcg atctcgccgg aggcgctgga gccgcgtgta ttgcggaagg gcattttcac
     1681 caaagacgtg gaagatcagg cagccatagc caaacgtcta agccacgcag tgaacgatgg
     1741 ctttgcagga accatcgcta tggcctctca gagcgcccag aacgcaaaaa gagcgcgtga
     1801 gctacagaag actatggatg cgcagcagaa acgcctacag agcgttacag agccttttaa
     1861 ggggctttcc agagagcaga tgaccgaaat cctgatgatg gctcagcggt ttaagcagaa
     1921 aaaccaggaa aaggaaaaac agcagcgtat tgaacgtgaa aaacagcgac agacgcgatc
     1981 gcgtggcatg ggtggtatgg agcgttagtt ttgatgcgat gcaggatagg gctaacgccg
     2041 caaagtgacg tcatttgcgg cattccagag atcaggttct gccgccctgt ataacgctga
     2101 ggccagctaa tccatcagat ggtaattgta ttttttcaaa atagaaaaag ctgcgttttt
     2161 ctgctttggt gacaaaccct tagttgagta tttattaccg ctagttctac gatatatgct
     2221 gtaaacactg cttaaaaaag tacgctccca aacagtcaga acctcattct tttttagtga
     2281 atcatgcagc attagcctgt ctacattttt aaaaagttta tcttcagcat tcatcatcca
     2341 ttaccttcgt ctgcaccgtt cttccattgg tgtcataact gatctttaac tcaattttct
     2401 ttcttctttc gtaaattgat ggccagtctc cccgtgcaaa aagaaagggg ttagcttcaa
//
