LOCUS       CP099117                4074 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB35-SO-C06 plasmid unnamed3, complete
            sequence.
ACCESSION   CP099117
VERSION     CP099117.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488620
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB35-SO-C06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 134x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:25:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,070
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,821
            CDSs (with protein)               :: 4,821
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 51 of 131
            Pseudo Genes (incomplete)         :: 92 of 131
            Pseudo Genes (internal stop)      :: 22 of 131
            Pseudo Genes (multiple problems)  :: 29 of 131
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB35-SO-C06"
                     /isolation_source="Pooled soil sample collected from floor
                     of cattle farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(103..357)
                     /locus_tag="NFK56_25340"
     CDS             complement(103..357)
                     /locus_tag="NFK56_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WFZ22142.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobilization protein"
     gene            536..2683
                     /locus_tag="NFK56_25345"
     CDS             536..2683
                     /locus_tag="NFK56_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WFZ22143.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            complement(2942..3643)
                     /locus_tag="NFK56_25350"
     CDS             complement(2942..3643)
                     /locus_tag="NFK56_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WFZ22141.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(359..535)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          536..894
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2943..3643)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          450..2680
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccgtgcgtat gtgctcaacc tggcgactga ttcacttccg cttcctcgcc cactcgctcg
       61 ctaacgctcg gtcgctgggg ctgcggcgag cggacgatac aatcactgtt ctggcagcgc
      121 ctccagctcg ttaatcagcg gcagtatgcg cgtcacatca gcctgtcgtg tcacctctgc
      181 cttcaggaac tggagtaacc aggcacgcaa ctgcacctcc tccggctttc tgacctttgc
      241 cagcaatgca gcacccacaa caaccttctg cgctgtgtcc aggctccggc tcttcgcctt
      301 caggcgctgt aatctcgcct cagcatcagc aatcttctgt tcgagcgttc tgctcatgat
      361 gtaactccgt gcgcgatgaa aaatcgcatt ttagcgcgtc actggtagtt taaaaactga
      421 gctggcataa tgcacggcaa atcacgaagt gcgcacttat acaatctcca ctccgtttcg
      481 attgtgtgcg ccctgcgggg ctgaaagaaa actacaaaaa ggcattacgg cagaaatggc
      541 gatttatcat ctcagcatga aaattatctc cagaagcagc ggctacagcg ccgtagcttc
      601 cgctgcctat cgttccggtt cactgatgct tgatgagcgt accggactga ctcacgacta
      661 cacccgcaaa agcggtgtgg ctgaggctgt gattctgacc cccgccaccg cccctgcctg
      721 gtgtacgaac cgtgccgaac tctggaatgc cgttgagaag gccgaacgcc ggaagaactc
      781 acaacttgcg cgggaaattg aactcgccat cccccacgaa ctgccgcagg atgccgcacg
      841 ggaaaccgtt ctcgcctttg tcagagaaaa ctttgtcagt cagggcatga ttgctgatgt
      901 ggcgttccat cacatggata aaacaaatcc ccatgcgcac atcatgctga ccacgagggc
      961 tatcggacct gctgggttca gcggaaaggt cagggactgg aacgaccgga cacatgccga
     1021 gacgtggcgg gcatcatggg ctgaccatgc aaacagggca ctggcgaacg ccggttatca
     1081 ggaagagata gaccatcgtt catacgagcg acaggggctt gagaaaacgc ctggtattca
     1141 cctcggaaag tctgcctgtg cgatggaaac acgcggtatc gaaacagaac gcggcgaaca
     1201 gaatcgtctg attaacaggc ttaacctgga aatacagatt tcccgcaccg aactcaggaa
     1261 cggagggctg acagagcctg cccgccgtgt ggctgacctt ctgaatatcg ggcttcctga
     1321 caacgcaact tcgtacacac tacgtgacat tatcgaagcg ttgccgaagg acagtaactc
     1381 ggcatggaaa ctcaccagta atgccatgtc gatgatggca gatatgcagg cgacgcgcag
     1441 acgctgggaa gagctgaaca ccgaacgaaa ggaagcgatg agccatgctg ccagcctgaa
     1501 aaaatcatcg ccgttttcgt ccggtttttc ccgtattccc ctgatgcaat gggcggcacc
     1561 ggaataccgt agtgagcagg gaaaaatcca gtctctgagc cagcaaatgg aaaaattacg
     1621 acagcattac cggaaggtgg agaaacagga cattcccgcg agtcagatgg catttgaaaa
     1681 acagtggcag cagtgggggg aatccggcct tgcagctctc agagcccagt tgcttaaacg
     1741 tgaagaggaa ttgcgccgga aggaacagga agatactgaa cgtagtcgcg cggagcagct
     1801 caggaaaaat gacaatgccg ttatggattc aggggtgctg agagcagtcg ttacggggta
     1861 tggcgaggct cccatgcctg gtaacgggga aatgacctgt taccttctgc ttcacaaccg
     1921 gaacggagaa tatacgctct gggggaacga gctggagaaa taccgcacca gaatctttga
     1981 gagtgttgat ttaatgcgtg acaggagtgg ttatatctgt gaccgaagcg aaatcggcca
     2041 acgtccgcca ctgcaacgtg tttattcctc tgcaaccttt gagcaactgc tgacgcaggt
     2101 ttgccagaca tggccgcaac atacccgcaa tctccgccag ccgaagacat ggccggagtc
     2161 tttctgtctt ggagaagacc gccagccagc catgccatca ccagcggcaa ggaaggtgga
     2221 ttttatgcag ggacgtctgc caccgacgct gatgccggtc atgagttctg tcgaccggga
     2281 aacccgccag cttcagcttt tgctggtcat gggcgtggat gactctctgg gcggtgttat
     2341 ccggcttaat ggtacgctgt atcccgcgtt tgccgttccc tcagccgata acaggcatct
     2401 ggtcatcagt gcgctgaccg ataaggggtt acggtatgca gggtatggtg ttgccgtaaa
     2461 tcacgatgca gacagccaca tatcacctgc accagaactg atgcagtttc atctgaaaac
     2521 ccgtgaggca ccgctttttg ctgccgtcaa cacaccagaa aaacagccgg atcatctgtt
     2581 ccgctcgctg ggttttaatc gtacctggga ggaatggcag cgcgaggaag atgcacgaac
     2641 acacaccacc gagaggagac atgatcgcgg ttggtcacag tgaatgtgaa caatattttg
     2701 ttttgtttaa catcgtgtta tgttcgtcga tgttcttcga tgttcatcga cagggatgaa
     2761 tgcaagaagc acactcacat aacgcaagca tagttttgtt tttccgcccc caaaaattgg
     2821 gggggtcttt tttcgctgca tcagagcgtt tttgacgcaa tttttcactt caatagtacc
     2881 ttcgcactac taacacaaaa aaaacgcagc agggagcgtt ttaggtgcct taaaatcgat
     2941 tttagatcgt catcttgatc tgtttgtctt ccttgaagcg aaaatgcagg gctacaaccg
     3001 tgcgcccctt tttgatcgtc tcaacagtta caaccagatc gcttttttcg ttcagttcgg
     3061 cgatggctgg cttgattatc atcttattca agtctttgaa ttgttgatat ttatcttcaa
     3121 cggcaagcat ggttcggaaa tcatcgatag ctatcgttct gtcaccggtg ctgcggaact
     3181 gctggagcag ctcgtaaatg cggatgctgt aggtgctgga aaggctggag acgttcttca
     3241 cgactacccg cgtaaactgc cccttgagct gtgtcagata cggcatgatg gcatcggaga
     3301 aggtgatatc tactttcgcc tcacctttga aatactgcgc ccggtactgc acccagcgga
     3361 actcttctcg tttttcctcg ttcttgatga ttactgacct gtcccagagg cgatctatcg
     3421 cttccttgag cctagcttca gcgtgctgcc gtcccatgtc aggaaaggca tcatgaaact
     3481 ctgcagccgt gatggtcatg gttttttgtt catccggcga aggggaatct tctccggatt
     3541 ttaggcggct gatacagacc agcaggaagc gctgttcctg caaagtgagc ttgtagcttg
     3601 cctcgataag ggcgtttgat ttgtaagcta tcagatctga catttcgctt gctcgttgct
     3661 attggtggcg tttttttact gtacgccact tatcacatgg gtggaagttt tttttttgca
     3721 accccctttt tctgtggatt acacccccaa aagctccacc tttcaccccc aaaagctcca
     3781 cctttcaccc ccaaaagctc cacctttcac ccccaaaagc tccacttatc ttgccagaac
     3841 atctttaaaa tcatggtgtt acaagaccta aaaaggtttt aaaaaaaaag atctaaaaat
     3901 aagatcttaa aaacttaacg gcgagcctgt ggataacttg gggtgctgaa aacaaaaaac
     3961 caataaaaac aatgcgttgc aataaagcag aattgcaagt ttcgaataca ccccctccaa
     4021 ccctgtggaa acccagtatt catgcgggtt cagcgcatat cacctgccga aaaa
//
