LOCUS       CP099111                6811 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB40-SO-C05 plasmid unnamed3, complete
            sequence.
ACCESSION   CP099111
VERSION     CP099111.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488629
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6811)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB40-SO-C05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:33:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,174
            CDSs (total)                      :: 5,056
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 71 of 163
            Pseudo Genes (incomplete)         :: 101 of 163
            Pseudo Genes (internal stop)      :: 40 of 163
            Pseudo Genes (multiple problems)  :: 44 of 163
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB40-SO-C05"
                     /isolation_source="Pooled soil sample collected from floor
                     of cattle farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            48..1211
                     /gene="cna"
                     /locus_tag="NFK65_25760"
     CDS             48..1211
                     /gene="cna"
                     /locus_tag="NFK65_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin colicin N"
                     /protein_id="WFZ41891.1"
                     /translation="MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWS
                     WSNKPHKNDGFHSDGSYHITFHGDNNSKPKPGGNSGNRGNNGDGASAKVGEITITPDNS
                     KPGRYISSNPEYSLLAKLIDAESIKGTEVYTFHTRKGQYVKVTVPDSNIDKMRVDYVNW
                     KGPKYSNKLVKRFVSQFLLFRKEEKEKNEKEALLKASELVSGMGDKLGEYLGEKYKNVA
                     KEVAGDIKNFHGRNIRSYNEAMASLNKVLANPKMKVNKSDKDAIVNAWKQVNAQDMANK
                     IGNLGKAFKVADLAIKVEKIREKSIEGYNTGNWGPLLLEVESWIIGGVVAGVAISLFGA
                     VLSFLPISGLAVTVLGVIGIMTISYLSSFIDANRVSNINNIISSVIR"
                     /name="cna"
     gene            complement(1220..1744)
                     /gene="cni"
                     /locus_tag="NFK65_25765"
     CDS             complement(1220..1744)
                     /gene="cni"
                     /locus_tag="NFK65_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061352107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin N immunity protein"
                     /protein_id="WFZ41892.1"
                     /translation="MDIKDRNKISKKISFSLLLLLSPFALIFFSYNNAPIPLLEKIIAY
                     LSLPGFHSSNNPPLSEAFNLYVHTAPLAAISLFIFTHKELELKPKSSPLRALKILTPFT
                     ILYISMIYCFLLTDTELTLSSKTFVLMSKNDLFLSFFYITLYIGIYIFTYLYLWFFIGT
                     YKLFTRGGILP"
                     /name="cni"
     gene            1772..1909
                     /locus_tag="NFK65_25770"
     CDS             1772..1909
                     /locus_tag="NFK65_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WFZ41893.1"
                     /translation="MCGKILLILFFIMTLSACQVNHIRDVKGGTVAPSSSSRLTGVEIK
                     "
                     /name="colicin release lysis protein"
     gene            complement(2928..3119)
                     /locus_tag="NFK65_25775"
     CDS             complement(2928..3119)
                     /locus_tag="NFK65_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WFZ41894.1"
                     /translation="MTKQEKTVLNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3184..3507
                     /locus_tag="NFK65_25780"
     CDS             3184..3507
                     /locus_tag="NFK65_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WFZ41895.1"
                     /translation="MITIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGEELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3716..4147
                     /locus_tag="NFK65_25785"
                     /pseudo=""
     CDS             3716..4147
                     /locus_tag="NFK65_25785"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4185..4703
                     /locus_tag="NFK65_25790"
     CDS             4185..4703
                     /locus_tag="NFK65_25790"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WFZ41886.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            4710..4943
                     /locus_tag="NFK65_25795"
     CDS             4710..4943
                     /locus_tag="NFK65_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WFZ41887.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5299..5469)
                     /locus_tag="NFK65_25800"
     CDS             complement(5299..5469)
                     /locus_tag="NFK65_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFZ41888.1"
                     /translation="MSKAPIGTTASTGQKCPESGVWEVVGTPSTTAPIAKGNVMPPYNG
                     KAVTWKLTQYA"
                     /name="hypothetical protein"
     gene            complement(5663..6082)
                     /locus_tag="NFK65_25805"
     CDS             complement(5663..6082)
                     /locus_tag="NFK65_25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WFZ41889.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQHFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(6163..6600)
                     /locus_tag="NFK65_25810"
     CDS             complement(6163..6600)
                     /locus_tag="NFK65_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WFZ41890.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGFVSYRTGQDGRR
                     EFETSELIRAYGELKQNETPERHSTGQAENPHDQPTERILRELNELKQCLTLMLEDKQA
                     QDMDRKRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     Typing          2756..2842
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="100.0"
                     /evalue="1.13e-36"
     Typing          complement(2188..2745)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3498..5047
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.901"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtatttattt ttaatggttt gttttaaaag tcaaagaggg atttattatg ggtagtaatg
       61 gcgcagataa tgcacataac aatgcttttg gtggagggaa aaatccgggc attggtaata
      121 ccagtggcgc aggaagtaat ggtagtgcat caagtaaccg aggaaattcc aatggatggt
      181 catggagtaa taagcctcat aaaaatgatg gcttccacag tgatggttct tatcatatta
      241 catttcatgg ggacaataat tcaaagccta aacctggagg gaatagtgga aatcgaggta
      301 ataacggtga tggagcgagc gctaaggttg gagagataac aatcacacct gacaactcga
      361 aaccaggtcg ttatatttcg tcaaatcctg aatattcatt gttggcaaaa ttaattgatg
      421 cggaatcaat taaaggtaca gaggtatata cttttcacac cagaaaaggt cagtatgtta
      481 aggttactgt tccagatagt aatattgata aaatgagagt tgattatgtg aactggaagg
      541 gaccgaaata tagcaataaa cttgtgaaga ggtttgtgag ccagttttta ttatttagga
      601 aggaggaaaa agaaaaaaat gaaaaagaag ccttgctaaa ggctagtgaa cttgtgtctg
      661 gtatggggga taaacttggc gagtatcttg gagaaaaata taaaaatgta gctaaggaag
      721 ttgccggtga tattaaaaac tttcatggcc gtaatattcg tagctataat gaagcaatgg
      781 cttcactgaa taaagtgtta gcaaatccaa agatgaaagt aaacaaatct gataaggatg
      841 ccattgtgaa tgcctggaaa caggttaatg ctcaggacat ggctaataag ataggtaatc
      901 ttggcaaggc atttaaggtt gctgatttag ctataaaggt tgagaaaatt agggaaaaaa
      961 gcattgaggg atacaatact ggcaactggg gacctctcct gctggaggtt gaatcatgga
     1021 tcatcggggg cgttgttgct ggagttgcta ttagtttgtt tggggctgtg ttgagttttc
     1081 tcccaatctc aggacttgct gttactgtgt tgggggtaat aggaataatg acgattagtt
     1141 acttgtcatc tttcattgat gcaaatcgag tttcgaatat aaataacatt atatctagtg
     1201 ttattcgatg acatgagtgt caggggagta tacctcccct ggtaaatagt ttatatgttc
     1261 ctataaagaa ccaaaggtat aaatatgtga atatatatat cccaatgtat agtgttatat
     1321 agaagaaaga caaaaacagg tcattttttg acattaatac aaatgttttt gatgataagg
     1381 ttagttctgt gtcagttagc aagaaacagt atatcatgga tatataaaga atagtgaaag
     1441 gagttaatat ctttagcgcc cgcagaggtg atgactttgg ttttaactct aattctttgt
     1501 gtgtgaatat gaataagctg attgcagcta aaggggctgt atgaacatag agattgaatg
     1561 cttcgcttag gggtgggttg tttgatgaat gaaatcctgg tagggatagg tatgcgatga
     1621 ttttttcaag gagtggtatt ggtgcattat tataactgaa aaatattaat gcgaatgggg
     1681 aaagtaagag cagaagactg aatgatattt tttttgatat cttatttctg tcttttatat
     1741 ccatgttgct ttctttggat ggttggaaat tatgtgtggg aaaattctat taatcttgtt
     1801 ttttatcatg acacttagtg catgtcaggt caatcacatc cgtgatgtga aaggagggac
     1861 tgttgctcca tcatcatcat ccagactaac aggggttgaa attaagtaaa agatcaaagg
     1921 atcctcttga gatccttttt ttctgcgcgt aatctgctgc ctgtaaacga aaaaaccgcc
     1981 tggggaggcg gtttagtcga aggttaagtc agttggggac tgcttaaccg aggtaactgg
     2041 cttcagcaga gcgcagatac caaatactgt cctttcagtg tagccgcagt tgggccatca
     2101 cttcaagaac tctgtaagca tctggataaa tctcgctctg ctaattcggt taccagtggc
     2161 tgctgccagt ggcgttaagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2221 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2281 gacctacacc gaactgagat accaacagcg tgagctatga gaaagcgcca cgcttcccaa
     2341 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2401 ggagccacca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2461 acttgagcgt cggtttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2521 cggcgtggcc ttctctgggt tcttgttttt tgctcacatg tttcttttgg tgttatcccc
     2581 tgattctgtg gataaccgca ttaccgtctt tgagtgagct gataccgctc gccgcagccg
     2641 aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa gagcgtcgga agttgcattt
     2701 tctccttacg catctgtgcg gcatttcaca cccggcatgg cgcacttttc atacaatccg
     2761 cactaatgcc gcatggttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     2821 gctgcgcccc gacacccgct aaaacccgct gacgcgccct gacgggcttg tcagctcccg
     2881 gcatccgctc acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttct
     2941 ccgtcatccc cgaaacgtgc gaggcagctg cggtaaagct catcggcgtg gtcgtgaagc
     3001 gattcacaga tatctgcctg ttcatctgcg tccagttcgt tgagcttctc cagcagcgtt
     3061 aatgtctggc ttctgataaa gcgggccatg ttaaggacgg ttttttcctg tttagtcact
     3121 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgcgag
     3181 aggatgatca cgatacgggt tactgatgat gaacatgctc gtttgctgga acgttgtgag
     3241 ggtaaacaac tggcggtatg gatgcggcgg gtctgtctgg gtgagccggt tgcccgttcc
     3301 gggaggctgc cgacactggc accaccgtta ctgcgtcagc tggccgccat cgggaataat
     3361 ctgaatcaga cagcccgtaa ggtgaacagc gggcagtggt cttccggtga ccgggttcag
     3421 gtggtggccg cactgatggc catcggggag gagctgcgcc ggctgcgtct ggccgtcagg
     3481 gaacagggga cgcgggatga tagttaagtt tcatgccagg ggaaaaggtg gtggcagtgg
     3541 tccggttgat tacctgctgg gaagggagcg taaccgggaa ggggcaacgg tgcttcaggg
     3601 taatccggaa gaagtccggg aactcatcga tgccacgcca tttgcgaaga aatacacgtc
     3661 cggtgttctg tcgttcgcgg agaaggaact gccgccggga ggacgtgaaa aagtgatggc
     3721 gagttttgag cgtgtactga tgcccggtct cgaaaaaaat cagtacagca tcctgtgggt
     3781 ggagcaccag gacaagggac ggcttgagct gaattttgtc attccgaaca tggagctaca
     3841 gagcggaaaa cgcctccagc cgtactacga ccgggcagac aggccgagaa ttgatgcctg
     3901 gcagacgctg gttaatcacc attacgggct gcatgacccc aacgccccgg agaaccgccg
     3961 gatactgact ctccctgata acctgccgga aacgaaacag gcgcttgcag agagcgtcac
     4021 acgcgggata gacgctcttt atcatgtggg ggagataaaa ggccgtcagg atgtgattca
     4081 ggcgctcact gaggcggggc ttgaagtggt cagggtgacg cgaagcagca tcagcattgc
     4141 agacccgaac ggcgggaaga atatcaggct gaaaggagca ttttatgagc aatcttttgc
     4201 agacgggcgc ggagttcgag aaaaagctga aagagagagc cgaatctaca gagaaaatgc
     4261 tgaacaacga gttcaggagg ctcggcgaat ctgtaagcga ggctgtgaca tcaaacgaga
     4321 cgaaaatcag agacgctata gccctgttca cggcctcgac agaggaatcg ctggaaaaac
     4381 accgggaagg ggtgaaagag gcgatgatgc agcacaggag ggacgtgtta aagctggcag
     4441 ggaatacggg catgatgtta ctggggatag tctttctcct gtttaccgcg agtggcggga
     4501 cgctctggta tcttggcggg aggatacagg cgaacctgga agaaatcagg aagcaggaag
     4561 agacattgca gaaactgaac gcgaagacat ggggcgtgga gtttgtgcag gacgggaaca
     4621 ggaaattcct tgtccttccg tacgggaaat cagcggaggt gattcccttt caggggaaag
     4681 agtgggtaca tctgaaggag tgacacagag tgacagagct ggaaacacat ttgctgaacg
     4741 ccttagagca gctgcaacag gactatatgc agcggctgag cgaatgggag agcgccttcg
     4801 tggaattgca gaagatgttt tcgcttacgc aacgggacaa cgcgatgctg aacgagcggg
     4861 tcatgcagtt gagtcagcag gtgcagcact tgagcgagca gacagaacgc ttgagccagt
     4921 tatacagcga gaactggaga taagagagga acggttgata caggagcgcg aacatgtgtt
     4981 atccctggaa cgggagcgtc agccggaaat acaggaacgc acgctggatg gcccttcgct
     5041 gggatggtga aaccatgaaa aatggcagct tcagtggatt aagtgggggt aatgtggcct
     5101 gtaccatctg gttgcatagg tattcatacg gttaaaattt atcaggcgcg atcgcggcag
     5161 tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg atgaacgcat
     5221 tatggcggtg cgtacaatta agggattatg gtaaatccaa gaataacgaa aaagcccccg
     5281 gtgggggctt ttgagatatc aagcgtattg ggttaatttc caagttacgg cttttccgtt
     5341 atacggaggc attacattgc ctttggcaat tggtgctgta gttgaaggcg ttcctaccac
     5401 ttcccaaaca ccgctttcag ggcatttttg tccggttgaa gctgttgtac caattggggc
     5461 tttagacata ttgattcctc ttgtatgttt tatccgtatg cttgtgtagc aatacttatt
     5521 tctggtttag aaatcagctt tcaagcgggc ttaaaggtcg ttttttcaca gatgtgattg
     5581 tggtcatatt tttaacaaag ttgtatatgc ttttggcgtt gtaagttttg gttttgttat
     5641 aaaggactta tggaaaatca acttactgcc tgccctcgta gccatcgaga taaacagccg
     5701 aaatggtgtc agccagcagc ctggattttt tcggggtgag gtctttgctg cggtaatcgg
     5761 tacccgcaaa ttgtgttgtg ccccggagtg agtttaaaaa ttcctgactg tgaaagtgct
     5821 gaatgcgttg ctgcacatca ctccgtgatt ttcctgtcgc tttatcttta gctcccatat
     5881 cccagaactg ccgaaaaaaa ggcaccatca ttcgtgttgt gtcggccttc tgggtggaaa
     5941 agttaggatc aaaaccgtca tcagaagcat atacgtaatg ccgggtatgt tgttccacgc
     6001 tgccctttgg ggtacaggct gtgagagcta ctgttgctgt tatcatggcg ataagacaca
     6061 gtttgtttcc ggtacgtctc atgagtgtct ccctaccttc aagtgtttat tctcggtgag
     6121 ttattatccg aagactattt tatcatcagt ctctgacagc gttcagcgac cgaacagcct
     6181 ggaccagaat ccccgtttct tttctagttc cagtgcctgc ctgagctggg ctatctcatt
     6241 ctgtagctgt tcccgttctg cttcctggcg tttgcgatcc atatcctgtg cctgtttgtc
     6301 ctcaagcatc agcgtcaggc attgtttcag ctcattcagc tcccggagaa tgcgttctgt
     6361 cggctgatca tgtggatttt ctgcctgtcc cgtactgtgc ctttctggtg tctcattctg
     6421 ctttaattcg ccgtatgccc ggatcagttc actggtttca aactcccgtc gtccgtcttg
     6481 accggtgcgg taactgacaa agcctgcgcg catatcacgg taaaactgtg aacgcgatct
     6541 gcctgtcatt ttcagtgcgt cacggagtgt gtgccatgcc ataaagtgac agtgtcccat
     6601 aggtgtctca tctcatggtt tcagtaaaac ataatgaggt ctgagaacgg taatgtttgt
     6661 gctggttttt gtggtatccg gcgggaatag cacgtggtgt ggctggctgt ttttttgatc
     6721 gttttcacaa aaacgaaaat tcccagggtt gacaggaaaa acgcagcggc gtaattttaa
     6781 actgtatata aaaccagtgg ttatgtgtac a
//
