LOCUS       CP099100                6231 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB41-E2-C07 plasmid unnamed2, complete
            sequence.
ACCESSION   CP099100
VERSION     CP099100.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488633
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB41-E2-C07
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 129x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:20:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,699
            CDSs (total)                      :: 4,581
            Genes (coding)                    :: 4,446
            CDSs (with protein)               :: 4,446
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 59 of 135
            Pseudo Genes (incomplete)         :: 74 of 135
            Pseudo Genes (internal stop)      :: 31 of 135
            Pseudo Genes (multiple problems)  :: 27 of 135
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB41-E2-C07"
                     /isolation_source="Pooled sediment sample collected from
                     floor of pig farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(5836..6231,1..138))
                     /locus_tag="NFK69_23455"
     CDS             complement(join(5836..6231,1..138))
                     /locus_tag="NFK69_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFZ59628.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQNLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVILED
                     KLAEKPVTGKNRKN"
                     /name="hypothetical protein"
     gene            complement(141..1068)
                     /locus_tag="NFK69_23460"
                     /pseudo=""
     CDS             complement(141..1068)
                     /locus_tag="NFK69_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            1245..1643
                     /locus_tag="NFK69_23465"
     CDS             1245..1643
                     /locus_tag="NFK69_23465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFZ59629.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQAVQNRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(1904..2923)
                     /locus_tag="NFK69_23470"
     CDS             complement(1904..2923)
                     /locus_tag="NFK69_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WFZ59630.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(3420..3563)
                     /locus_tag="NFK69_23475"
     CDS             complement(3420..3563)
                     /locus_tag="NFK69_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WFZ59625.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(3625..3885)
                     /locus_tag="NFK69_23480"
     CDS             complement(3625..3885)
                     /locus_tag="NFK69_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WFZ59626.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            complement(3888..5615)
                     /locus_tag="NFK69_23485"
     CDS             complement(3888..5615)
                     /locus_tag="NFK69_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="WFZ59627.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPIYVSV
                     TDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPGKATGKGKP
                     VGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFKDSNK
                     TNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
                     /name="colicin"
     BGC             1..6231
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2149..2923)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(353..1068)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.908"
                     /coverage="100.0"
                     /evalue="1.67e-172"
ORIGIN
        1 aatatcccga tatccgcgaa gtgtttccct tgccacagcc agagaatcag caacagcttt
       61 ctgttgctcc attgcggtat gttgcaaatg ctccaccgat aacaggagcg tgagtaattt
      121 ttcttctgtg ctggccatgt tcagaatccc atagaaaatc cacgtgacgg tgatttaacc
      181 cgttcaggtt cagttcggtc ctgtctgctc actctgtctc tcatgtcaaa gccatgctcc
      241 cgcgcaacat catgttgcag ccttctgacc agttcatgtt gcttaaccgg agtgtccagc
      301 cattccatga tgtcatgccc gaccgttcgc gcctgttcaa gatggcgggt aatagtccgg
      361 ttcagaccgt ttttgcatga tgtcatgacg ctgggcatca tcaagccgtc cggcagcttc
      421 aaaacccaga tgcacttcgg gacgtcgttc aatgccctgt tctttcagcg tccgacagtc
      481 aatgaagccc ctttcaaagc cattttttct catatgttcg ttgtgcttca cctcccagcg
      541 ttttcgcaat tcccgcaact gctctttttg ctgagtggga gtcaggcttc ctgaatcctt
      601 tttcgcacct ccccgctcgg gatacctggc gttatagcgc cggaaatact gttccggtgt
      661 acgttcaatg ccatcattca cacgctgact catcatgata tgcgcatgag gctgttcgcc
      721 tccctcaatg ctcgctttcg gattgtgaat ggcaaaactc catgcatgcc gttcgccagc
      781 ttcctgacgt acaaaatcct gcaccagttc aaggcgctgt tccggtgtca gttcacgggg
      841 cagcgctatc tccagttccc gataagtcga accgtttgca cgttcaaact ggtcagccgc
      901 ctgccagaaa tgtgagggat tatctcgcgc ccattccggc atgtttccgt gtgcggaata
      961 ttccaaatcc tgacggtcgc ggtatttctc tttgcgctcg atgtaatcgg cgtggtttgc
     1021 agcctgcccc ttgccaccaa atttaacgct gaggtgatag gttgccacgg tttcagccct
     1081 ccgcaggagc gcactgtttc tcgacgaagg agagaaacat ataagtgcgc ttccttctaa
     1141 ttttacgaat tagcacggaa gataccccct gcacccgccg accgcaagcg atatactgca
     1201 cgaccacaac acaccgcaaa aaaacaccag ggagcaccac gaacatgcgc atcagcaaca
     1261 tcgaatggct gaagaaacgg atcggattca tcaggaagct cggagaacag accgcacgac
     1321 agagacagat tattgacctg ctcgacaacg aagcaggact cacagaacag gaacgcaaac
     1381 tcctccacgt actggcaaca gcggaaaaaa acgacttaca ggcacaggag aacgaacgaa
     1441 aacaggcggt ccaaaatcga atcgagggaa aaaaacaacg gcgagaacgc aatcaccgac
     1501 tctttctcgc ggctgggcta ctgatcgaag caggactggt agacacaaaa acaggcgagc
     1561 tatgctataa aaaagacagg atattacagg cacttaagga aattaagtac gatctggaga
     1621 catccccaaa cccagacgcg taagtgcctc catccgtgtt ccggttccct ggtgcttcct
     1681 cgctcattcg ctcgctacgc tcggtcatga gactgcggcg agcacgacca cctcaatcat
     1741 gcggtaagcc gcaccattgc tgggctgaga ggagaaaacc gccggaaagc ccgctgctca
     1801 aaaagacgcc gaaaggcgct gttatctgat ataggcttgt tcatgtccgc gaatttaaca
     1861 gtccattaac aaaaaacggg gggtagtgtt gcttctttgt taactaacat ttaattattt
     1921 gtttcttggt tagccattct ccagaattat ccatccagcg tcgaatagtc cggctgctaa
     1981 caccaaccgc cactgcaata tcttccgttg acgctcccaa gatatacata tcaagcgccc
     2041 tacgacgttt gtcgtcgtta gcagtccacc gaacagcggc gctttttttc cctttgtccg
     2101 actgattttc tgacgaattg gccttcccgc ccttgcgacc gcgtgctgcc tgaatttctg
     2161 gcgtgtgcgt atcagccaca tactgtgcga aagtttccgg cgtgaagttc ctgtgcgtgt
     2221 atttcgcaat gctcttgcca atagcccgac attcagccag tgaaagcgga acgggaagcg
     2281 atgcattgta catttcgaca cgctggatca ccgcttcaag ccactgtgag aacgctggcc
     2341 agccctgacg aatcgccctg taagcccatt tgcgcgtcat ttcgaacagg tggcagtttc
     2401 gccccatccc gtaatgttta tcgatgctac gacgctctga ggcgctcaaa tcgagataat
     2461 ctgccagttc atcgagggta taggcgtcct cgcgccactc catcacctgc cattccaggt
     2521 ggaacggatt tttgcaaatc aggccgctgt agttcacatc agcacaaagt ttctcacaca
     2581 gcgaacgttc aaccgctgcg gcatatttca gcgccttaac cgaggcatca ggcgcggttc
     2641 tcacggcgat gttgagtgcg tagagcaggt gagcgtgtcc gttcacagga tttttaacgg
     2701 tgatgttcgg cgcgggggcg ttccggtcgc tccagtcaat cgcagccccc tcgcggtcaa
     2761 cgtcaaaaac cagccagaac tgcgcatgag gctggttctg ctggatgtac cgcgcaagaa
     2821 gcgcacgccc tttgccggag atgcgaaggc cgaaagcgag atcatccgtg tgatagggac
     2881 gatggggtaa attttcgtcg aagtattgaa gcgcggcgct catgtgttac gattctctcc
     2941 gggttatgcg acctacacgc cctctgtatt tcatgttgaa tcttgccgga tctggacatg
     3001 atttcagggg cgtttgtttt tcagccgctt acactacacc caacggcaaa tcctgtcgat
     3061 ccttcccgaa aaaccgatcc atttaccata actcccgttg tacgcacccc ttaaaacgcc
     3121 ctcagtcgct ttctgacgca aaaaacgcaa aaagacaaga aaacaccagg ccaaaccaga
     3181 aaaacgcgcc agaatcgttt ttagagggct gcataactat gcacgaaagc gatgcgcgat
     3241 cgatttcgac tgttccgaag ggcgaacagg tcaaaatgac aagttttctc gccctgggtg
     3301 gaaagttctg cacggggatt ttagctctca tctcccgcat ggaatttttg tgacatttca
     3361 aaaaaaagcg catcagaatc agcctgacac gctttgtttt tgtcaaagag gatttcgggt
     3421 tactgcgttg ctaatccggt cacttcagct gttgatgacg gggatacagt cccgccctga
     3481 acatcccgga tatagtttgc ctgacatgca gccagaataa tgactgcaag aagcaataaa
     3541 ataatccctg ttattttttt cataacgact ccttgttgtg aaagaatgcc agccattcat
     3601 ccataaacaa ccggcattca tattttagcc ctgtttaaat cctggcttac cgttagcagc
     3661 tcgccattct ttaatttctt taacaatccc ttcaggacta tcttccctgt catcgcgagg
     3721 ataataaatc agatctgaac catctgggtg ctcagttaat cgctcaaact ctctcactaa
     3781 tttattgtca tcctcttcag tagcaccttc agctctacat atttttttta caaactccag
     3841 aaattcagcc tcggtataat cactaatact atgtttcagt tccattttta cttacctcta
     3901 tgaatatcaa tatgtcgctt aggtgtggtc actctgatat tatccatatc atagacacca
     3961 ccatcctgac tgattggttt atcatgatgt aattcaaagc gttccctacc acctacttgg
     4021 tctttcttcc ttgcaaaagg tgcttttccc ttttgaatgt tcgtcttatt actgtcttta
     4081 aattgcttac taagctcggg atcttttgac acttcttccc agaatttctt ccggaaatcg
     4141 tcaaaacttt taaattcttt atcacgcaac ttatcagcaa tgcgatcagg aattggcgct
     4201 cctgaatctt tacccgcatc atcaagccat ttatcaccaa ctggttttcc tttacccgtc
     4261 gccttccctg gcttattccg tttactctcc ttatctaatt tatccttaga gtccttttct
     4321 ttattttcct tctgcttacg gcgttccagc gcagcaccta acgcagcatc agcatcagac
     4381 ttatcttttg cagcagcatc aaatgcagcc ttcttattat ttacatccgt ctgtgcccgc
     4441 tgggccttaa gccctgccat ttgccacatt cggtgaccag cagccattgg ttctcgggca
     4501 aatcgctcga attgttttat ttcagccttt gcatcagcaa gagttttatt cgcagcatca
     4561 agttcacttt tacgagaatt ataaacctga acagctttag cctgtcgttc ctggtttctg
     4621 gcaacatctt tattagcctg atccagttct gcgcgggcct gttcataatt gcgttcagcc
     4681 acttctacag gatgtgtgtc attccattcc tgctggaggc gattttcctc atcctgacgt
     4741 tgttttacct gtgcaggggt aagaacatct gtcactgata cataaatcgg cttctgccca
     4801 ctttctttcg gaaaacggat caccgcttca tgtgaaccac caccaaaggt aaatcctgca
     4861 ggaacggcag tccggccttt atcctccgta ataccacggg gaagggttgt tgacaccgga
     4921 aggcctttga cagtgctgac tgtcagagaa gggacaccag gaaatgatgc gtgaaataca
     4981 cctggagtac gtgttggttt ggcattcaca acaggcacac tcataggcac acctgcaact
     5041 acggcaatat gctgtcgtgt gtccttcaca acatctgtca cacgtttcgt cacgctgaca
     5101 gttgcctggt ccagcggaag tgtactgacc tgaacattcg tcactgtttc tgccggtaat
     5161 gacgtcacaa tctttgacat catattcggg tcatcttttg ctatttcaga tggcagaatg
     5221 ccgtaaagcg caatacccca tgcgctgaat ttaaacggac ctttcagggc tgcaaagata
     5281 tcagctattg ccgcagataa agcctcacca gagacggcga tacccagcgt tccggcacca
     5341 ggagttgcca aagcaggaaa accaaatgcc actgctgcta cggatgaatt gctgccacca
     5401 ccggaattac cattcccccc gccattacca ttgccggagc cacctcccca gtgaacacca
     5461 ctaccggaac cgccacccca tggattattt tccgaactcc atcctgaacc gtcagaagcc
     5521 ccaccatttc caccaagccc cgtagggccg ccattaatgt taccacctgt gttatgtgcg
     5581 ccactgttgt ggtcgcgtcc atctccaccg ctcataaaaa ttccctcttt taagcgttaa
     5641 aacaattgat tacatagtta atactgtaca taaaaccact ggttttatgt acagatcaaa
     5701 aagtactcgc cattattttt tctgtcaagt cagggaggga tattttttgg cagttcggag
     5761 gaatcactca tgaaaacggt gaacggaacg cgaaatcctt tcgctgtccg tgaggcgttg
     5821 gacaagcgaa gcgcgtcagt ttttccggtt cttacccgtg acaggttttt ccgccagttt
     5881 atcttccagt atcacaactt tctgatacac cgccgctgtg gcgttgtagt tcctgtcgag
     5941 cattgttttc attccccatc cgagcacaca gaacataact agaaaagcca ctgacacccc
     6001 acccagccat ttccagctat accctttaag ggtgttgtat tcctgcacga tattgctgtg
     6061 aattgtcgca atatccctgt tcagggcatc cgcgtttgcc ttcatgtttg cgactgactc
     6121 ttccagccgc tgaaaatcct ggctgatgcg ctcacctatc agccctgcta cgtccagatt
     6181 ctgcacctca tttctgactt catccctgac ctgttttgcc acaccgtcag a
//
