LOCUS       CP099049                3433 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB44-C14 plasmid unnamed3, complete
            sequence.
ACCESSION   CP099049
VERSION     CP099049.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488664
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB44-C14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 137x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:47:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,824
            CDSs (total)                      :: 4,702
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 80 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 35 of 137
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB44-C14"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            1..441
                     /locus_tag="NFL00_24100"
     CDS             1..441
                     /locus_tag="NFL00_24100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WGA59336.1"
                     /translation="MSKDTQRITQINDQGEIVGGFVAVIRPKQKSSFQRHFTMNQDALK
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIADALEMQKTHVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="MarR family transcriptional regulator"
     gene            complement(505..1521)
                     /locus_tag="NFL00_24105"
     CDS             complement(505..1521)
                     /locus_tag="NFL00_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WGA59337.1"
                     /translation="MRSPDWLDVRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENHCSVSQSADEAMGKLRELLPEKRRKDAVLCVEYVMTASPEWWDKAT
                     PRQQIEFFARSERWLEEKYGKDRVISSVVHRDEATPHLSAFVVPLTKDGRLSAKEFIGG
                     RAKMRNDQTTYAESVKMLGLERGIEGSRATHQTVQHYYESLNRGVRNQVSISPEALEPR
                     VLRKGIFTKDVEDQAAIASRLSQAVNEGFAGTVAVASQSAQNAKRAKELQKTMEAQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRVEREKQRQMRSRGMGGMER"
                     /name="plasmid recombination protein"
     gene            2165..2647
                     /locus_tag="NFL00_24110"
     CDS             2165..2647
                     /locus_tag="NFL00_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGA59334.1"
                     /translation="MNTKKISKLERHTYMDESGEIKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFLRAGRGELEANPFLFARGDWPSIYERRKKIELTISYDSNGRTVQTTVTDDER"
                     /name="hypothetical protein"
     gene            2634..2864
                     /locus_tag="NFL00_24115"
     CDS             2634..2864
                     /locus_tag="NFL00_24115"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGA59335.1"
                     /translation="MMNAEDKLFKKVDGLMLHDSLKKNEVLTVWERTFLSSAYSIYRRT
                     SGNEFSTKGLSPKQKSTVFSILKKYNYHLMD"
                     /name="hypothetical protein"
     Typing          complement(509..1521)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.941"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3016..3317)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.079"
                     /coverage="100.0"
                     /evalue="2.35e-50"
ORIGIN
        1 atgagcaagg acacccagag aatcacccag attaacgatc aaggtgagat tgttggcggc
       61 ttcgtcgcgg taatcagacc caagcaaaaa tcgtcatttc agaggcattt cactatgaac
      121 caagacgcat taaagatcct cgctaaggag ctaacgggcg agcaatttaa agttctaatg
      181 ctcatgctcg ctgatctgga ttacgagaat ttcatccaaa ttgcacaggc tgatatagcg
      241 gatgcattgg agatgcaaaa gacccacgta agccgcgctg ttcgtgctct tcttgatgta
      301 ggcgtgatct ttgaaggtcc gaaagtaggt agatccaaaa cctatcggtt gaacgagcaa
      361 ttcggctgga aaggaacagt aagcaatcac aaaaaagccc ttaaaaacgg cctcagcgtt
      421 atacagggcg gcagaacctg atctctggaa tgccgcaaat ggcgtcattt tgcggcgtta
      481 gccctatcct gcatcgcatc aaaactaacg ctccataccg cccatgccac gcgatcgcat
      541 ctgtcgctgt ttctcacgtt cgacacgctg ctgtttttcc ttctcctggt tttgctgctt
      601 aaaccgttga gccatcatca ggatttctgt catctgctct ctggaaagcc ccttaaaagg
      661 ctctgtaacg ctctgtaagc gtttttgctg ggcttccata gtcttctgta gctctttcgc
      721 tcttttcgca ttctgggcgc tctgagaggc cacagcgacc gttcctgcaa acccttcgtt
      781 cactgcctgg cttaaacgac tggcgatcgc tgcctgatct tcaacgtctt tggtgaagat
      841 acccttccgc agtacacgcg gctccaatgc ctccggtgag atcgatacct ggttacgcac
      901 accacggttc agtgattcgt aatagtgctg cactgtctgg tgtgtcgccc tgctgccttc
      961 aatgcccctt tccagaccga gcattttcac cgactccgca tatgtcgtct ggtcattccg
     1021 cattttcgcc cgcccgccaa taaactcttt tgcgctcaac cgcccgtctt tcgtcagagg
     1081 aaccacaaat gcagacaggt gcggcgttgc ttcatcccgg tgaacaaccg acgagataac
     1141 cctgtctttg ccgtatttct cttcaagcca gcgctccgag cgggcaaaga attcaatctg
     1201 ctgccgtggt gtagctttgt cccaccactc agggcttgcc gtcatgacgt actcgacgca
     1261 caacaccgca tctttacggc gtttttcagg caacagctcc cgcagctttc ccatcgcctc
     1321 atctgccgac tgagacacag agcagtggtt ttctggtgtc cgttcagcgt cagcattcgg
     1381 cgtctcacgc tccctgtagc agtgctgcaa agcgctggcc acactgccca ttcccgtcag
     1441 ctttttgcag cgcattatcg cgtaattcgc catgcttacc ccctcgttct ggcgtacatc
     1501 cagccagtcg ggggatcgca ttgcgatgcc tccggcgggc cactatcagg atgatagtgt
     1561 actcactaga ctttgctacg caaaatcgtt cgggaagctg cgcttccaaa ggtcaaaatc
     1621 aaaagcgtct gacaggtgga aggcctcact gcgtgaaccc ttccaccctg accagcttgt
     1681 ttttgtccgt gtgtcatcaa gggtgaaatg cactcgctcc gctcgccctt gacgaaatca
     1741 ggatctggat atcgcttttg aaagagcagg aaccgctcgc cgcagatcgg attagggagc
     1801 ggcaacgccg cgagtctgtc cgatcgagga agcggaattt ccactccccg gacatagaaa
     1861 tccgtacgct ccagactcaa aaaagctcgt ttttcgatgt ttaaaaatgg cgtttttcag
     1921 cctttatttt cccccttaat atatagatat agatacggga acccgcgcac accaagggct
     1981 agagagaaga atcgtactca cctcaagtac taatcgtact cacctcaagt actaatcgta
     2041 ctcacctcaa gtactaatcg tactcacctc aagtactaat cgtactttag gtaaggtact
     2101 tgactacctg catctttagt acttaaatct gagtactaaa atcatgctga tacatggggc
     2161 tagagtgaat acaaaaaaga tttctaagct tgaacggcat acatacatgg acgagagcgg
     2221 agagattaaa caagactcta agatcgagac tttttctatc ccggctgaac cgccctacgt
     2281 caagctttac atcgcagaca ttgcgatgat tcatggtttg tcaaaatctc aaaatgatgt
     2341 gcttttgcag ttggcaatgc tgattggatg ggatggcttt gtatctgtat caaaaactag
     2401 atttgagaag accatacaac ctagggttca tattgaatat cagacgttta aaaacataat
     2461 cgctgccctt gttgataaac aaatctttct ccgggcaggg cggggagagc ttgaagccaa
     2521 tccgtttctt tttgctcggg gagactggcc ttcaatttac gaaagaagga aaaaaataga
     2581 gttaacaatc agttatgatt ccaatggaag gacagtacaa acaacggtaa cggatgatga
     2641 acgctgaaga taagctattc aaaaaagtag atggattaat gctgcatgac tctctaaaaa
     2701 agaatgaggt tttgactgtt tgggagcgta cttttttaag tagtgcatac agcatatatc
     2761 gcagaacaag cggtaatgaa ttttcaacta agggattgtc accaaaacaa aaaagcaccg
     2821 ttttttctat tttgaaaaaa tacaattacc atctgatgga ttagctggac tcagcgttat
     2881 tgaggcggga gggtttaacg cccccgatcc ctgcgtgtgt gctggcggtc tttctccgcc
     2941 ttatccctga acttctccag aatctggtca cggtttctca gttgcttttc ccggttctgc
     3001 tcacggaacc gggaagccat tcccagtatt tcgtccatct gctcgcgtga aagcccctta
     3061 aaaggctctg taacgctctg taggcgtttc tgctgcgaat ccatagtctt ctgtagctcg
     3121 cgcgctcttt ttgcattctg ggcgctctaa gaggccatag cgatggttcc tgaaaagcca
     3181 tcgttcactg cgtggcttag acgtttggct atggctgcct gatcttccac gtctttggtg
     3241 aaaatgccct tccgcaatac acgcggctcc agcgcctccg gcgagataga tacctggcta
     3301 cgggtgccac gcgttataag atacagaagg agcgataaaa aaacgtatgt atggcgaggg
     3361 gttacatttg acatagttac caataatggt aacactgtta ccaataatgg taactcaaga
     3421 taggttattt tcg
//
