LOCUS       CP099048                5145 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB44-C14 plasmid unnamed2, complete
            sequence.
ACCESSION   CP099048
VERSION     CP099048.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488664
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB44-C14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 137x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:47:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,824
            CDSs (total)                      :: 4,702
            Genes (coding)                    :: 4,565
            CDSs (with protein)               :: 4,565
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 62 of 137
            Pseudo Genes (incomplete)         :: 80 of 137
            Pseudo Genes (internal stop)      :: 34 of 137
            Pseudo Genes (multiple problems)  :: 35 of 137
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB44-C14"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            670..1578
                     /locus_tag="NFL00_24075"
     CDS             670..1578
                     /locus_tag="NFL00_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WGA59331.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPEFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGRSIAKYTHRKFSPEGFSAVQAARGRKGGIAKGEAYEDKRFMALCMLENGYSQ
                     KAIAAMLNVHRNTIRNWAMHK"
                     /name="replication initiation protein"
     gene            complement(1771..2169)
                     /locus_tag="NFL00_24080"
     CDS             complement(1771..2169)
                     /locus_tag="NFL00_24080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGA59332.1"
                     /translation="MRISNIEWLKKRIEFIRKLGKQTARQRQIIDLLDNEDRLTEQERK
                     LLHVLATAEKNDLQAQESERKQAIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELRYKKDMLLQRLKPIKYDLDTCPNPDA"
                     /name="hypothetical protein"
     gene            2346..3272
                     /locus_tag="NFL00_24085"
     CDS             2346..3272
                     /locus_tag="NFL00_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WGA59333.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSGNLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYYRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDIAREHGFD
                     MRDGVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            3275..3808
                     /locus_tag="NFL00_24090"
     CDS             3275..3808
                     /locus_tag="NFL00_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGA59329.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISLDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3874..4083)
                     /locus_tag="NFL00_24095"
     CDS             complement(3874..4083)
                     /locus_tag="NFL00_24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGA59330.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLRKLPPDHTIPPDLTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          671..1398
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.61"
                     /coverage="88.0"
                     /evalue="0.0"
     Typing          2347..3269
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agcgtagcta cttcatcgtt gttcgaacac aaccaacaac ctgtaattgt tgtttctctt
       61 ggttccccac ttttgtgggg ttttttatct cttccgtgca tcgtaaagcg cggactcctt
      121 ctggaaaggt tgttttaggg catcactgag gcattttttg cgtttgtgaa tattgagcat
      181 ccgataacta aaatatttcc catagactct gattcttgcc accgacccca ggtcggtttt
      241 ttttgtcctg gcgagatgtg aagtttggaa acaggaaacc ccgtcagagc gccacgcacc
      301 aggggagaaa acttgtcgtt ttgagccgtt cggtgttcag aacgcacgaa accgatcgca
      361 catcgctttc gtgcatagtt atgcaacgcc ctgaaaacga ttctgacgca tttttctggt
      421 tcagcctggt gtttccttgt ctttttgcgt tttttgcgtc agaaagcgat tgagggcgtt
      481 ttatggggtg cgtacaacgg gagttatggt aaatggatcg gatttgcggg aaggctcgac
      541 aggttttgtc gttgggggta gtgtaagcga ctgaaaaaca aacgcccctg aaattcgtgt
      601 ctatccgcca agactttcac gaaaatacag gggcgatgtc ggtcgataac ctggaaggaa
      661 tcgtaacaca tgagcgccgc gcttcaatac ttcgaagaaa atttacccca ccgtccctat
      721 cacacggatg atctcgcttt cggccttcgt atctccggca aagggcgcgc gcttcttgca
      781 cggtatatcc agcagaacca gcctcatgca cagttctggc tggtttttga cgttgaccgc
      841 gagggggctg cgattgactg gagcgaccgg aacgctcccg cgccgaacat caccgttaaa
      901 aatcctgtga acggacacgc tcacctgctc tacgcactca acatcgccgt gagaaccgcg
      961 cctgatgcct cggttaaggc gctgaaatat gccgcagcgg ttgaacgttc gctgtgtgag
     1021 aaactttgtg cggatgtgaa ctacagcggc ctgatttgca aaaatccgtt ccacctggaa
     1081 tggctggtga tggagtggcg cgaggaagcc tataccctcg atgaactggc tgattatctc
     1141 gatttgagcg cctcagcgcg ccgtagcatc gataaacatt acgggatggg gcgaaactgc
     1201 cacctgttcg aaatgacgcg caaatgggct tacagggcga ttcgtcaggg ctggcctgaa
     1261 ttctcacagt ggcttgatgc cgtgattcag cgtgtcgaaa tgtacaacgc atcgcttcct
     1321 gttccgcttt caccagctga atgccgggct attggcagga gcattgcgaa atacacgcat
     1381 agaaaattct cgccagaagg gttttccgct gtacaggccg cacgcggtcg caaggggggg
     1441 atcgctaaag gcgaagccta cgaggacaag cgtttcatgg cgttatgtat gctggagaat
     1501 ggatattctc agaaagctat tgcggcgatg ctgaatgtac atcgtaatac gatccgtaat
     1561 tgggcaatgc acaaatagcc ttatcagata acagcgcctt tccggcggtt ttttgagcag
     1621 ctattttttg ggtggtttct gcttgtcagc ccagcaatgg tgcggctttt cgcttgattg
     1681 aggtggtagt gctcgccgca gtctcatgac cgagcgtagc gagcgaatga gcgaggaagc
     1741 accagggcac cggaacgtga acgggaacac ttacgcgtct gggtttgggc atgtgtctag
     1801 atcgtactta atgggtttaa gtctttgtaa taacatgtct tttttatagc gtagctcgcc
     1861 tgtttttgtg tccaccagtc ctgcttcgat cagtagtcct gccgcgagaa agagccggtg
     1921 attgcgttcc cgtcgttgtt tcttcccctc gattcgtttc tggatcgcct gttttcgttc
     1981 gctctcctgt gcctgtaagt cgtttttttc ggctgttgct agtacgtgga gaagtttgcg
     2041 ttcctgttct gtgagtctgt cctcgttgtc gagcaggtca atgatctgtc tctgtcgtgc
     2101 ggtctgtttt ccgagcttcc tgatgaactc gatccgtttc ttcagccatt cgatgttgct
     2161 gatgcgcatg ttggtggtgc tccctggtgt ttttttgcgg tgtgttgtgg tcgtgcagta
     2221 tatcgcttgc ggtcggtggg tgcaggggtt accttccgta ctaattcgaa aaattagaag
     2281 gaagcgcact tatatgtttc tctccttcgt tgagaaacag tgcgctcctg cggagggctg
     2341 aaaccgtggc gacctatcac ctcagcgtta aatttggtgg caagggacag gctgccaacc
     2401 atgccgatta catcgagcgc aaagagaaat accgcgaccg tcaagatttg gaatattccg
     2461 cacacggaaa catgccggaa tgggccaggg ataatccttc ccatttctgg caggcggctg
     2521 accagtttga acgtgcgaac ggttcgacct atagggaact tgaaattgca ttaccccgtg
     2581 aactgacacc ggaacagcgc cttgaactgg tgcaggattt tgtacgtcag gaagctggcg
     2641 aacggcacgc ctggagtttt gccattcaca atccgaaagc gagcattgac ggcggcgaac
     2701 agcctcatgc acatatcatg atgagtcagc gtgtgaatga tggcattgaa cgtacaccgg
     2761 aacagtattt ccggcgctat aacgccaggt atcccgaccg gggaggtgcg aaaaaggatt
     2821 ccggaaacct gacccccact cagcaaaaag agcagttgcg ggaattgcga aaacgctggg
     2881 aggtgaagca caacgaacat atgagaaagc atggctttga aaggggcttc attgactgcc
     2941 ggacgctgaa agaacagggc attgaacgac gtcccgaagt gcatctgggt tttgaagctg
     3001 ccggacgact tgatgatgcc cagcgtcatg acatcatgca aaaacggtct gaaccggact
     3061 attaccgcca tcttgaacag gcgcgaatgg tcgggcatga catcagggaa tggctggata
     3121 caccggttaa gcaacatgaa ctggtcagaa ggctgcaaca tgatattgcg cgggaacatg
     3181 gctttgacat gagagacgga gtgagcagac agaaccgaac tgaacctgaa cgggttaaat
     3241 caccgtcacg tggattttct atgggattct gaacatggcc agcacagaag aaaaattact
     3301 cacgctcctg ttatcggtgg agcatctgca acataccgca atggagcaac agaaagctgt
     3361 tgctgattct ctggctgtgg caagggaaac gcttcgcgga tatcgggata tttctgacgg
     3421 tgtggcaaaa caggtcaggg atgaagtcag aaatgaggtg cagaagctgg acgtagcagg
     3481 gctgataggt gagcgcatca gcctggattt tcagcggctg gaagagtcag tcgcaaacat
     3541 gaaggcaaac gcggatgccc tgaacaggga tattgcgaca attcacagca atatcgtgca
     3601 ggaatacaac acccttaaag ggtatagctg gaaatggctg ggtggtgtgt cagtggcttt
     3661 tctggttatg ttctgtgtgc tcggatgggg aatgaaaaca atgctcgaca ggaactacaa
     3721 cgccacagcg gcggtgtatc agaaagttgt gacactggaa gataaactgg cggagaaacc
     3781 tgtcacgggt aagagccgga aaaactgacg cgcttcgctt gtccaacgcc tcacggagcg
     3841 cgaaaggatt tcgcgaaccg ttcaccgttt ttttcaatat gattctgtct gctttggtcg
     3901 ataaagcagt cgatagtatt ccagccttcg tatatataac ggtacgtgct catcagtaag
     3961 atctggcggt attgtgtgat cagggggaag ttttctcaga tactcacgaa atgcctggct
     4021 tgatgccata aaatctacag tagcatcgat aatatgtggg gtgttttcac ctaattttgc
     4081 catgatgata atccttatat tatcacggtt cgtgaatgat agggggtcac tattatttat
     4141 gcgttagtaa accagaaaaa atcccccgtt ggcatagggg gatatgtaag gagaaataac
     4201 ttcgcaggtg ttcttgtttg tataacgtga aaagactgca cgcaacttaa taagtctgcc
     4261 gtacttactt tcagttttca acataaggtt tataacaatg tatggaatgg aagtgagctt
     4321 taaggtttca gctctaaaaa ataaaatatt tattagttgg ttggttgttg ttcttgttct
     4381 gtatgtatat aaacactatc gacatattat gaccgggaac attccttacc ccctctgcgc
     4441 tctcagaggg ttttttttgt cattaattaa taatgaactg aaatgcagtt ccgatacctc
     4501 catttgcaca ttatccctga catatggtac cggaaccgca ttaattttat gttataagag
     4561 tttattttta atgtccagat ggtttaatgg tatttatatg taattagctg tatggatagt
     4621 actgttttta ttgtataact tgttgttttt tattgttttt gtgtggtgtg ctaatcttta
     4681 agttataaaa aaaccccttg gaacacaggg gcaaaagtaa ggaaaacaaa tggatgctca
     4741 gtttgtgtga cactttaaac taatggaact atagagtgga ggttgttaca gtcgctttta
     4801 taaaacagca accagttttt ttcaatagtc tgatgatggt tgtgacagtt ttgtgatatt
     4861 gttcaaaatg tttcttgttg aatgtttgtt ttttggtcta ttggtgtctg gttttatttt
     4921 ttctgctatt acatatcaag aaatgtgatt ggtgtagcaa gagtggaatg cgatgtggat
     4981 aaactaattg gttgtcgaat ctaatttact tagaaaacta cacttgtttt tgtgtatgaa
     5041 tttagtgaat ttgtctgtct atgtgttttc gtgcatttat aaatccttcg tcaggatctt
     5101 aaatttagtg ttgaatatgt agggcctgta cggtaattat tattt
//
