LOCUS       CP099045                4074 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB44-C17 plasmid unnamed4, complete
            sequence.
ACCESSION   CP099045
VERSION     CP099045.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488665
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB44-C17
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:25:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,905
            CDSs (total)                      :: 4,782
            Genes (coding)                    :: 4,609
            CDSs (with protein)               :: 4,609
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 73 of 173
            Pseudo Genes (incomplete)         :: 111 of 173
            Pseudo Genes (internal stop)      :: 38 of 173
            Pseudo Genes (multiple problems)  :: 41 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB44-C17"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            75..329
                     /locus_tag="NFL01_24515"
     CDS             75..329
                     /locus_tag="NFL01_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WGA64143.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     gene            861..1553
                     /locus_tag="NFL01_24520"
     CDS             861..1553
                     /locus_tag="NFL01_24520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WGA64144.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1811..3970)
                     /locus_tag="NFL01_24525"
     CDS             complement(1811..3970)
                     /locus_tag="NFL01_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WGA64145.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTELARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     Typing          complement(3613..3971)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          3972..4074
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.038"
                     /coverage="100.0"
                     /evalue="3.58e-45"
     Typing          862..1553
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1815..4057)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.064"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attatgccag ctcagttttt aaactaccag tgacgcgcta aaatgcgatt tttcatcgcg
       61 cacggagtta catcatgagc agaacgctcg aacagaagat tgctgatgct gaggcgagat
      121 tacagcgcct gaaggcgaag agccggagcc tggacacagc gcagaaggtt gttgtgggtg
      181 cagcattgct ggcaaaggtc agaaagccgg aggaggtgca gttgcgtgcc tggttactcc
      241 agttcctgaa ggcagaggtg acacgacagg ctgatgtgac gcgcatactg ccgctgatta
      301 acgagctgga ggcgctgcca ggacagtgat tgtatcgtcc gctcgccgca gccccagcga
      361 ccgagcgtta gcgagcgagt gggcgaggaa gcggaagtga atccgtcgcc aggttgaaca
      421 catacgcacg gtttttcggc aggtgatatg cgctgaaccc gcatgaatac tggatttcca
      481 cagggttgga aggggagtat tcggaatttg caattctgct ttcttgcaac acattgtttt
      541 tattggtttt ttgttttcag caccccaagt tatccacagg ctcgccgtta agtttttaga
      601 tcttgttttt aagatcttgt ttttaatact tgttttaggg gcgaaattca ttaataaaat
      661 caatatgtta tagtggtata tgtggaggaa tttaaggtaa aggtggagga atttaaggtg
      721 tttaggtgga ggaatttaag gtaaaggtgg aggaatttaa ggtgttattc acagaaatgt
      781 gaggatataa aaaaaaatat tccacatttc ttatttgtgg tgcacattaa agcatcacca
      841 catttgaacg ggatctttgt atgtcggact tgattgccta caagtcgaat gctttggttg
      901 aggccagcta caagttgacc ctacaggaac aacgttttct gttgctgtgt atcagtcgat
      961 tgaaatctgg tgctgatgct gaattgcaaa agacaatgac cattaccgct gcggaatatt
     1021 ttgattcatt ccctgacatg gggcggaaaa atgccgaggt tcagttacag gaagcgatcg
     1081 atcgtttatg ggacaggtcg atcattctca agaatgacga gaagcgtgag gagttccgct
     1141 ggatccagta tcgggcacag tacgctaaag gtgaggccaa ggctcgaata accttttctg
     1201 acgcggtaat gccttatctg acgcaactga aggggcaatt cacgcgcgtt gttataaaaa
     1261 acatatcagg tttatcaagt agttacagta taagaatata cgagttcctc cagcaattcc
     1321 gcagtacggg agagagaatt attgctctta atgactttcg ttcaatgtta gggattgaaa
     1381 ataaatacaa acaattcaga gacttaaata aaatactaat aaaaccctgt gttgacgaac
     1441 tgaacaaaaa aagcgacctg gctgtaacgg ttgagacgat caaaaagggg cgcacggttg
     1501 tagccctgca ttttcgcttc aaggaagaca aacagatcaa gatgacgatc taaaatcgat
     1561 tttaaggcac ctaaaacgct ccctgctgcg ttttttttgt gttagtagtg cgaaggtact
     1621 attgaagtga aaaattgcgt caaaaacgct ctgatgcagc gaaaaaagaa cccccaattt
     1681 ttgggggcgg aaaaacaaaa ctatgcttgc gttatgtgag tgtgcttctt gcattcatcc
     1741 ctgtcgatga acatcgaaga acatcgacga acataacacg atgttaaaca aaacaaaata
     1801 ttgttcacat tcactgtgac caaccgcgat cgtgtctcct ctcggtggtg tgtgttcttg
     1861 catcttcctc gcgccgccat tcgtcccagg tacgattgaa acccagcgag cggaacagat
     1921 gatccggctg tttttctggt gtgttgacgg cagcaaaaag cggtgcctca cgggttttca
     1981 gatgaaactc cataagttcc ggcgcaggtg atatgtggct gtctgcatcg tgatttacgg
     2041 caacaccata ccctgcatac cgtaacccct tatcggtcag tgcactgatg accagctggc
     2101 tgttatcggc tgagggaacg gcaaacgcgg gatacagcgt accattaagc cggacaacac
     2161 cgcccagaga gtcatccacg cccatgacca gcaaaagctg aagctggcgg gtttcccggt
     2221 cgacagaact cattaccggc atcagcgtcg gtggcagacg tccctgcgta aaatccacct
     2281 tccttgctgc cagtgatggc atggctggct ggcggtcttc tccaagacag aaagactccg
     2341 gccatgtctt cggctggcgg agattgcggg tatattgcgg ccatgtctgg caaacctgcg
     2401 tcagaagttg ctcaaaggtc gcagaggaat aaacacgttg cagtggtgga tgttgaccga
     2461 tttcgcttcg gtcacagata taaccactcc tgtcacgcat taaatcaaca cgctcaaaga
     2521 ttctggtgcg gtatttctcc agctcgttcc cccagagcgt gtattctccg ttccggttgt
     2581 gaagcagaag gtaacaggtc atttccccgt taccaggcat gggggcctcg ccataccccg
     2641 taacgactgc gctcagtacc cctgaatcca taacggcatt gtcatttttc ctgagctgtt
     2701 ccgctcgtcg ctccgcgcga ctacgttctg tatcctcctg ctccttccgg cgcaattcct
     2761 cttcacgttt aagcaactgg actctgagag ctgcaaggcc ggatgccccc cactgctgcc
     2821 actgtttttc aaatgccgtc tgactcgcgg gaatgtcctg tttctccacc ttccggtaat
     2881 gctgtcgcaa tttttccatt tgctggctca gcgactggat ttttccctgt tcgctacggt
     2941 attccggtgc cgcccattgc atcaggggaa tacgggaaaa accggacgaa aacggcgatg
     3001 attttttcag gctggcagca tggctcatcg cttcctttcg ttcggtgttc agctcttccc
     3061 agcgtctgcg cgtcgcctgc atatctgcca tcatcgacat ggcattactg gtgagtttcc
     3121 atgccgcatt actgtccttc ggcaacgctt cgatgatgtc acgtagtgtg tacgaagttg
     3181 cgttgtcagg aagcacgata ttcagaaggt cagccacacg gcgggcaagc tctgtcagcc
     3241 cgctgttcct gagttcagtg cgggaaatct gtatttccag gttaagcctg ttaatcagac
     3301 gattctgttc gccgcgttct gtttcgatac cgcgtgtttc catcgcacag gcagactttc
     3361 cgaggtgaat accaggcgtt ttctcaagcc cctgtcgctc gtatgaacga tggtctatct
     3421 cttcctgata accggcgttc gccagtgctc tgtttgcatg gtcagcccat gatgcacgcc
     3481 acgtctcggc atgtgtccgg tcgttccagt ccctgacctt tccgccgaac ccagcaggtc
     3541 cgacagccct cgtggtcagc atgatgtgcg catggggatt tgttttatcc atgtgatgga
     3601 acgccacatc agcaatcatg ccctgactga caaagttttc tctgacaaag gcgagaacgg
     3661 tttcccgtgc ggcatcctgc ggcagttcac gggggatagc gagttcaatt tcccgcgcaa
     3721 gttgtgagtt cttccggcgt tcggccttct caacggcatt ccagagttcg gcacggttcg
     3781 tacaccaagc aggggctgtc gcgggggtca gaatcacagc ttcagccaca ccgcttttgc
     3841 gggtgtagtc gtgagtcagt ccggtacgct catcaagcat cagtgaaccg gaacgatagg
     3901 cagcggaagc tacggcgctg tagccgctgc ttctggagat aattttcatg ctgagatgat
     3961 aaatcgccat ttctgccgta atgccttttt gtagttttct ttcagccccg cagggcgcac
     4021 acaatcgaaa cagagtggag attgtataag tgcgcacttc gtgatttgcc gtgc
//
