LOCUS       CP099030                4820 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB45-C13 plasmid unnamed3, complete
            sequence.
ACCESSION   CP099030
VERSION     CP099030.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488687
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB45-C13
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 145x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:11:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,706
            CDSs (total)                      :: 4,584
            Genes (coding)                    :: 4,356
            CDSs (with protein)               :: 4,356
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 63 of 228
            Pseudo Genes (incomplete)         :: 160 of 228
            Pseudo Genes (internal stop)      :: 46 of 228
            Pseudo Genes (multiple problems)  :: 37 of 228
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB45-C13"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            121..1056
                     /locus_tag="NFL23_23485"
     CDS             121..1056
                     /locus_tag="NFL23_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WGB09628.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(1250..1648)
                     /locus_tag="NFL23_23490"
     CDS             complement(1250..1648)
                     /locus_tag="NFL23_23490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGB09629.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1824..2750
                     /locus_tag="NFL23_23495"
     CDS             1824..2750
                     /locus_tag="NFL23_23495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WGB09630.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKGEYAKRKDLEYTEHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVLDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGNLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERHPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2753..3286
                     /locus_tag="NFL23_23500"
                     /pseudo=""
     CDS             2753..3286
                     /locus_tag="NFL23_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(3402..4283)
                     /locus_tag="NFL23_23505"
     CDS             complement(3402..4283)
                     /locus_tag="NFL23_23505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="WGB09627.1"
                     /translation="MEYIDKSTLNSKEKIRQAIEKKIREIKGYTPRVGIFGSTGVGKSS
                     LCNALFGRDVAPVSNVAACTREIQEIHIKPEDGNEGGLTIIDFPGVGETLERDKEYYNL
                     YKENVAKLDLVVWVIKSDERAYAISQKVYNDILLPNIKKCPVVFFINQIDKIEPTEDDE
                     GELLWDKKKNEPTKEQLASVNEKILEVSTAFSVPASYIIPTSVKRKFNISKAMETIIDI
                     LPKEKKYALYREAGEEIKTEKMAESAEKGVWDTIKEWTGEAWDNVKDVVVEVVAATVVA
                     AGKKILSKLKFW"
                     /name="50S ribosome-binding GTPase"
     Typing          122..1056
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.158"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1825..2747
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.455"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgttgggggt agtgtaagcg actgaaaaac aaacgcccct gaaattcgtg tctatccgcc
       61 aagactttca cgaaaataca ggggcgatgt cggtcgataa cctggaagga atcgtaacac
      121 atgagcgccg cgcttcaata cttcgaagaa aatttacccc accgtcccta tcacacggat
      181 gatctcgctt ttggtcttcg catctccggc aaagggcgtg cgcttcttgc gcggtacatc
      241 cagcagaacc agcctcatgc gcagttctgg ctggtttttg acgttgaccg tgtaggagct
      301 gcgattgact ggagcgaccg gaacgctccc gcgccgaaca tcaccgtaaa aaatcctgtg
      361 aacggtcatg ctcacctgct ctacgcactc aatatcgccg tgagaaccgc gccggacagc
      421 tcggttaagg cgctgaaata cgccgctgcg attgaacgtt cgctgtgtga gaaactttgt
      481 gcagatgtga actacagcgg actgatttgc aaaaatccgt tccacctgga atggcaggtg
      541 atggagtggc gcgaggaggc ctataccctc gatgaactgg ctgattatct cgatttgagc
      601 gcctcagcgc gccgtagcat cgataaacat tacgggatgg ggcgaaactg ccatctgttc
      661 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gttggccagc attctcacag
      721 tggcttgatg ccgtgattca gcgtgtcgaa atgtacaacg catcgcttcc cgttccgctt
      781 tctccgcctg aatgtcgggc tattggcaag agcattgcga aatacacgca caggaacttc
      841 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca caccagaaat acaggccaag
      901 agaggccgga aaggtggtat cgctaaaggc gaagcctacg atgacaagcg tttcatggcg
      961 ctatgtatgc tggagaatgg atattctcag aaagctattg cggcgatgtt ggaggtttct
     1021 actcgaacca ttcgaaactg gaaaagcgga aaatagccta tatcagataa cagcgtcttt
     1081 ttggcgtgtt tttgagcagc aggttttttg tcgtttttca ccgttcagcc ttgcaatggt
     1141 gctgttttcc gcatgattgt gctggtagtg ctcgccgcag tctcatgacc gagcgtagcg
     1201 agcgaatgag cgaggaagca ccagggaatc ggaacacgga tggaggcact tacgcgtctg
     1261 ggtttgggga tgtctccaga tcgtacttaa gttccttaag tgcctgtaat atcctgtctt
     1321 ttttatagca tagctcgcct gtttttgtgt ctaccagtcc tgcttcgatc agtagcccag
     1381 ccgcgagaaa gagccggtga ttgcgttctc gtcgttgttt ttttccctcg attcgttttt
     1441 ggaccgcctg ttttcgttcg ctctcctgtg cctgtaagtc gtttttttcc gctgttgcca
     1501 gtacgtggag gagtttgcgt tcctgttctg tgagtcctgc ttcgttgtcg agcaggtcaa
     1561 taatctgtct ctgtcgtgcg gtctgttctc cgagcttcct gatgaatccg atccgtttct
     1621 tcagccattc gatgttgctg atgcgcatgt tcgtggtgct ccttggtgtt ttttgcggtg
     1681 tgttgtggtc gtgcagtata tcgcttgcgg tcggtgggtg caggggggta tcttccttct
     1741 aattcgtaaa attagaagga agcgcactta tatgtttctc tccttcgtcg agaaacagtg
     1801 cgctcctgcg gagggctgaa accgtggcaa cctatcacct cagcgttaaa tttggaggca
     1861 agggacaggc tgcaaaccac gccgattaca tcgagcgcaa aggggagtat gcgaaacgga
     1921 aagatctgga atacacggag cacggaaaca tgccggaatg ggccagggat aatccttccc
     1981 atttctggca ggcggctgac cagtttgaac gagcgaacgg ttcgacttat cgggaactgg
     2041 aaatcgcgct gccccgtgaa ctgacaccgg aacagcgcct tgagctggtg ctggattttg
     2101 tacgtcagga agccggcgaa cggcacgcct ggagttttgc cattcacaat ccgaaagcga
     2161 gcattgacgg aggcgaacag cctcatgcgc atatcatgat gagccagcgt gtgaatgatg
     2221 gcattgaccg tacaccggaa cagtatttcc ggcgctataa cgctaggtat cccgagcggg
     2281 gaggtgcgaa aaaggattcc ggaaacctga ctctcactca gcaaaaagag cagttgaggg
     2341 aattgcgaaa acgctgggag gtgaagcaca acgaacatat gagaaagcat ggctttgaaa
     2401 ggggcttcat tgactgccgg acgctgaaag aacagggcat tgaacggcat cccgaagtgc
     2461 atctgggttt tgaagctgcc ggacggcttg atgatgccca gcgtcatgac atcatgcaaa
     2521 aacggtctga accggactat tcccgccatc ttgaacaggc gcgaacggtc gggcatgaca
     2581 tcagggaatg gctggacact ccggttaagc aacatgaact ggtcagaagg ctacaacatg
     2641 atgttgcgcg ggagcatggc tttgacatga gagacagagt gagcagacag aaccgaactg
     2701 aacctgaacg ggttaaatca ccgtcacgtg gattttctat gggattctga acatggccag
     2761 cacagaagaa aaattactca cgctcctgtt atcggtggag catctgcaac ataccgcaat
     2821 ggagcaacag aaagctgttg ctgattctct ggctgtggca agggaaacac ttcgcggata
     2881 tcgggatatt tctgacggtg tggcaaaaca ggtcagggat gaagtcagaa acgaagtgca
     2941 gaggctggat attgcagggc tgataggtga gcgcatcagt caggattttc agcggctgga
     3001 agagtcagtc gcaaacatga aggcaaacgc ggatgccctg aacagggata ttgcgacaat
     3061 tcacagcaat atcgtgcagg aatacaacac ccttaaaggg tatagctgga aatggctggg
     3121 tggggtgtca gtggcctttc tggttatgtt cagtgtgctc ggatagggga tgaaaacaat
     3181 tctcgacagg aactacaacg ccacagcggc ggtgtatcag aaagttgtga cactggaaga
     3241 taaactggcg gagaaaactg tcacgggtaa gagccggaaa aactgacgcg ctctgtgagg
     3301 tgtttgaatt gttcgctgtt cacatcggat aaaacagatc ttggaaaaag aaacgttgat
     3361 aactaagatt caggatagta atcatcctga atcttaatga attaccagaa tttaagtttt
     3421 gatagtattt tcttacctgc agcaacaaca gttgcagcta ctacttcaac aactacatcc
     3481 tttacgttat cccaggcttc acctgtccat tctttaatgg tgtcccatac gcccttttct
     3541 gcgctttctg ccattttttc tgtttttatt tcttctcctg cttcgcgata gagagcatat
     3601 tttttctcct ttgggaggat gtctattatt gtctccattg ctttagaaat attaaacttt
     3661 cttttgactg atgtaggtat aatgtatgaa gcaggaacag aaaaggcagt acttacttct
     3721 agtattttct catttacact agctaactgc tcttttgtcg gctcgttttt ctttttatcc
     3781 caaagaagtt caccttcatc gtcttctgtt ggttctattt tatctatttg gtttatgaaa
     3841 aatacaacag gacatttttt tatattaggt agcaatatat cgttatatac cttttgagaa
     3901 attgcgtaag ccctttcatc agactttatt acccatacaa ctaggtctaa ctttgcaaca
     3961 ttctctttgt atagattata atattctttg tctctttcca aggtttcacc aacaccagga
     4021 aagtctatta tcgtaagtcc tccctcattt ccatcttctg gcttgatatg aatttcttga
     4081 atctctcttg tgcaagcagc gacgttactg actggggcta catcacgacc aaataatgca
     4141 ttgcataatg atgatttacc tacgccagtt gatccaaaaa taccgactcg tggcgtgtaa
     4201 ccttttattt cccttatttt cttttcgatt gcttgtctaa ttttttcttt gctgtttagt
     4261 gtcgatttat caatatattc cattttaaga acctatattg tcaagaaata ttacttataa
     4321 ataaaccacc tattttataa gtggtatttt attgtattcg ataaatgtta tctagagctg
     4381 tgttcaggtt gttgtgagac taaacctttt aagcgatttc ctaaaaaggt ggggtacatg
     4441 gagctcagaa aacaaactat cccaagactg ttttttagtc atgttgcttt ggagagaggg
     4501 cgggatgaac tatggcaatt attgtgtctg atgctgatat cgaactgttt tatccatcta
     4561 tgcaaggata tggatgagaa aatttgctgt tttgacccat tcgcccttct gaacggttga
     4621 aactgttcgc gaatcacttt tgtgcatagt tatgcagccc cataaaaata attctgacgc
     4681 atttttcggg gttgccctgg tgtttccttg tatttttgcg ttttttgcgt cagaaagcga
     4741 ttgagagcgt tttaaggggt tcgtacaaca ggagttatgg taaatggatc gtttttgcgg
     4801 gaaggctcga caggttttgt
//
