LOCUS       CP099002                1618 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB46-C05 plasmid unnamed6, complete
            sequence.
ACCESSION   CP099002
VERSION     CP099002.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488707
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB46-C05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:28:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,823
            CDSs (total)                      :: 4,706
            Genes (coding)                    :: 4,516
            CDSs (with protein)               :: 4,516
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 59 of 190
            Pseudo Genes (incomplete)         :: 126 of 190
            Pseudo Genes (internal stop)      :: 39 of 190
            Pseudo Genes (multiple problems)  :: 31 of 190
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB46-C05"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(92..529)
                     /locus_tag="NFL43_24110"
     CDS             complement(92..529)
                     /locus_tag="NFL43_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /protein_id="WGB63825.1"
                     /translation="MNTQNLIADHREKHDRENLGFTQVYPLGWKRLMALAKGNQGAFGL
                     YTFLAQHIDGACGAVVADQAFLAAQFDVTTRTVRNWISYLEEQKAIVKIPVAGRVCAYA
                     LNPHEVWKGYDNNKPTAAFLTKTLVSKDGEIKRRIMAMFSN"
                     /name="repA"
     gene            1036..1488
                     /locus_tag="NFL43_24115"
     CDS             1036..1488
                     /locus_tag="NFL43_24115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rep protein"
                     /protein_id="WGB63824.1"
                     /translation="MRKCSMWLPVLFRFLAHSLTTFGRWGCGERNHLPHVVGDSVVVLS
                     GMSRQIAPQTRSEALRRTKRVLNARQGMEGAKNAYRGFQEVRRADTPNQGLSGRQEPRQ
                     PPAKPICTEQAATRLVGGRRPSVLQRWGSGVLPRRFPVALKALPEP"
                     /name="rep protein"
     Typing          complement(950..1028)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.75"
                     /coverage="87.0"
                     /evalue="1.58e-25"
     Typing          1131..1382
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.466"
                     /coverage="97.0"
                     /evalue="3.95e-106"
ORIGIN
        1 gcgactttat ggttggcaat ggagcaatgc aggatagaca atcatggtca gaacgttaac
       61 acgttgccct gattgtctgc ctgcggctgg ctcaatttga aaacatggcc atgattcggc
      121 gcttaatttc cccgtctttg ctgactaagg tctttgtcag gaatgctgct gtaggcttgt
      181 tgttgtcata gcctttccag acctcatgtg ggttaagtgc ataggcgcaa actcttcccg
      241 caacaggaat tttgactatg gctttttgct cttctaggta tgagatccaa ttacgcacag
      301 ttcgagtagt cacatcaaac tgagcagcca gaaaagcttg gtccgctaca accgcaccac
      361 aagcaccatc gatatgttgt gccaaaaaag tatacaaccc aaatgcgccc tgatttcctt
      421 tggcaagagc catcagtcgc ttccagccta gtggatacac ttgagtgaat ccgaggttct
      481 ctctgtcatg tttttctcga tgatctgcga ttagattctg ggtattcata gcgattccta
      541 tcaaccggaa acagttgttc ctataatagg aaatttattt tccatatcaa tgcttatttc
      601 gttcctactt tcagaagtaa taggaaacac atgtatttcc tatcatgcgg aaatacatgt
      661 gtttcctatt agctaatatt tccacaaata aaaacaatga gttacatcat ccgctaattg
      721 ttatcttaag agcacgatgt ttcctaatgc acgatcgctt ggtttttgtt tttttggggt
      781 ttcggggcgc agccctgaac cagtcacgta gcgatagcgg agtgtatact ggcttactcc
      841 tctgcaagcc tcgacagcag tgatttttgc cagatctcgc gctcttcacg ttcaaccgcg
      901 ccatcatggt tttgccgtcc gttcaccgct aaaacgcctc aggaatcagg acaggatgtg
      961 caattgtaat accgtcacac gcgacgctat cgcaattgcc acttggtcag ggcttcaccc
     1021 cgacacccag catctgtgcg taagtgctca atgtggcttc cggttctctt ccgcttcctc
     1081 gcccactcgc tcactacgtt cggtcgctgg ggctgcggcg agcggaacca cctcccgcat
     1141 gttgttggtg attccgttgt tgttctttcg gggatgagtc gccagatagc ccctcaaacg
     1201 cgctcagaag ctctcagacg cacgaaacgg gttttaaatg cccgtcaggg catggaaggc
     1261 gcgaaaaacg cttacagagg gtttcaggaa gtcaggcgcg ccgatacccc taaccagggg
     1321 ctttcaggtc gccaggaacc gcgtcagccc cctgcaaagc ccatttgcac ggaacaggca
     1381 gccacgcggt tggtaggtgg tcgtagaccg tcggtgttgc agcgatgggg gtctggggtt
     1441 ctccccagac ggtttccggt tgcgctcaag gcgctgccgg aaccgtgaca gcaaggataa
     1501 aaattctccc tgcggaaatt gagcagggag ggtgaaaata actacctatc acttacgttt
     1561 gagttttgaa ctcaggatga atatgcattt ggtcgtgttg aatttgcaag gatcgaca
//
