LOCUS       CP099001                2257 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB46-C05 plasmid unnamed5, complete
            sequence.
ACCESSION   CP099001
VERSION     CP099001.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488707
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB46-C05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:28:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,823
            CDSs (total)                      :: 4,706
            Genes (coding)                    :: 4,516
            CDSs (with protein)               :: 4,516
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 59 of 190
            Pseudo Genes (incomplete)         :: 126 of 190
            Pseudo Genes (internal stop)      :: 39 of 190
            Pseudo Genes (multiple problems)  :: 31 of 190
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB46-C05"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(1818..2257,1..550))
                     /locus_tag="NFL43_24105"
     CDS             complement(join(1818..2257,1..550))
                     /locus_tag="NFL43_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WGB63823.1"
                     /translation="MKNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MALCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRLKDRKEFRPVQGWIRTTEVTRSS
                     DGSAHPHFHTLMMVPPSMLSGDGYVKHARWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLAGATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESD"
                     /name="protein rep"
     Typing          complement(1735..1815)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.341"
                     /coverage="89.0"
                     /evalue="5.64e-30"
     Typing          1901..2092
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.891"
                     /coverage="100.0"
                     /evalue="2.88e-86"
ORIGIN
        1 cagatagcat tgagggcggc accatcatca gggtgtggaa gtggggatgt gcgctgccgt
       61 cactgctgcg ggtgacttcc gtggtgcgta tccagccctg aacaggccgg aactccttgc
      121 gatccttcag tcgctggaaa gctgcattca tccggttcag catctcgccc agctccccga
      181 tcgcacagtt gcgcacggtc agggtcagga acatccagcg ggcatcaggg taatccgcca
      241 cgatacgcgg cagggactgg tagaaacggg cttgccacat cagggagcgc ctccactggc
      301 agacaggaca gtgccggaca cggcagaaat gcgcctcacg cagcctcaga cgcgtttctc
      361 cggtttcctt gagggtagac cacccgaaac gcaataaacc gccacagagg gccatacgcg
      421 ccccgtagcg ctcatattcg gccgcaagca ggtaaatccc tcccacgtcg tcagaaacgc
      481 ttctgtgagc gtcccagggc gcatctgagg gggaatagtc cgtgaggttc tggagttcag
      541 gatttttcat gcacaccacc cgattgtggg cgatggctca tctgacgtgt tatcattgcc
      601 atatcacctg tgttagccgt atcaccaatt cgaatctcta caggtggtca cgcccccgaa
      661 cgatgcccgt cgttgcgggg gtcgctttta acgcgaccac ttccgacaga atacttccaa
      721 acccatttcg gagcaaatgt gccctcaagc cttgcgggac aagggctgga ggccaatttg
      781 aatcccagag ttatccctta agtataaata caaggaacgc gcgcgagctt tgagcaaaaa
      841 ataaacgcaa aatcagcgct ttttcacaac caaaaacctc aacaacacca gaagcgggat
      901 cgcaatgcta agcgccgcat caatgcgatc aaaagcctca aaaaacatct tcacctccca
      961 caaagtttta taaatctatc tggtttgtga ttttcgcatc cgtgttttcg ttttgcagta
     1021 ctgccaattt aatttcatac gttaaaacca tatccagcag cagatctttc aaaccgcctc
     1081 gcaggtggtt ggtctcactg cgtgacacca accaccatcc ccagtgtttt ccccgtgacg
     1141 tcgtcaatca caaaatcgga acccacgcca aaggcgcggg accccgcgat tttctgtttg
     1201 acgagttcgg gaacggtgag cggtacgcgg aattttttcg cgtgccgtga ggcgttggcg
     1261 gggtgtcggg gcgcagccct gaccaggatg tttggagtac cgaccgtgtg gcgggaatac
     1321 aaacatgtat cttgtcccga ctccgcagcg gtttttttgt ttgttgaacc gactgtaggt
     1381 caccgggcaa acgttgcgaa atggcgtcag agacgtcact ttgcggcgtt tgccctatcc
     1441 tgcatcggag taaaacacgg ggatttcttg ttccgatttt tttgccggaa cgctggcaat
     1501 tttctgccgt tctgcgctcc tgcagctacc acggccgtgc aaaattctgg cgaacggaca
     1561 gcgaattatt ttgccatttt tgtcgcgcag ctccgccacg cgttcgacaa gctggcggca
     1621 cagaacaccc acgaagtgcg cacttataca aacttcactt cgtttcgttt gtcgtgcgat
     1681 ctgcgatgct caaaaaaccg agaaacggcg ggaattttcg ggacgggaat tttcgggaca
     1741 ggatgtgcaa ttgtaatacc gtcacacgcg acgctatcac aattgccatc tggtcagggc
     1801 tgcaccccga caccctgcta gtctgattca gttttatctt tcgtcggtga gcgacggtac
     1861 tttctgacct cggttttcca ctcgaacgcg atgcgtgaac cgtcatcgtc accctcggaa
     1921 atatcatccc caatcaccat gtcggcatcg gtttcctgat cgagcttcag tacgtctttg
     1981 agtgcaccgc cggtcgccac aaagcggcgc ttgtgcgtct ggcgggtcag ttcgaggaac
     2041 cattcaggat cggccaccat gtcagccggc ttcgtggaat atttcagggt ttcggccacc
     2101 gcaccgcgca ccagttcggc agtggcaccg gccagcgatt cgccatcctt tggcttacgg
     2161 ggttttactg cccggacatc aacattcggg tcgtaactga cccgcaggca ctcccgccag
     2221 agttcaaccc agcgagcatg ttttacgtaa ccgtcac
//
