LOCUS       CP098990                1619 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB46-C15 plasmid unnamed6, complete
            sequence.
ACCESSION   CP098990
VERSION     CP098990.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488711
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB46-C15
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 115x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:22:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,876
            CDSs (total)                      :: 4,755
            Genes (coding)                    :: 4,475
            CDSs (with protein)               :: 4,475
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 280
            CDSs (without protein)            :: 280
            Pseudo Genes (ambiguous residues) :: 0 of 280
            Pseudo Genes (frameshifted)       :: 77 of 280
            Pseudo Genes (incomplete)         :: 194 of 280
            Pseudo Genes (internal stop)      :: 58 of 280
            Pseudo Genes (multiple problems)  :: 44 of 280
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB46-C15"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(1384..1619,1..298))
                     /locus_tag="NFL47_24375"
     CDS             complement(join(1384..1619,1..298))
                     /locus_tag="NFL47_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rep protein"
                     /protein_id="WGB73061.1"
                     /translation="MCKCNTVTRDDIPFAIWSGLHPDTPHQCVRAQCGFRFSSASSPTR
                     SLTFGRWGCGERCHLSHVIGDSAVVLSGMSRQIALQTLSEALRRTKRVLNARQGMEGAK
                     NAYRGFQEVRRADTPNQGLSGRQEPRQPPAKPICTALAAIQLVGGRRPSVLQRWGSGVL
                     PRRFPVALKALPEP"
                     /name="rep protein"
     gene            725..1162
                     /locus_tag="NFL47_24380"
     CDS             725..1162
                     /locus_tag="NFL47_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /protein_id="WGB73062.1"
                     /translation="MNTQNLIADHREKHDRENLGFTQVYPLGWKRLMALAKGNQGAFGL
                     YTFLAQHIDGACGAVVADQAFLAAQFDVTTRTVRNWISYLEEQKAIVKIPVAGRVCAYA
                     LNPHEVWKGYDNNKPTAAFLTKTLVSKDGEIKRRIMAMFSN"
                     /name="repA"
ORIGIN
        1 tgccctgacg ggcatttaaa acccgtttcg tgcgcctgag agcttctgag agcgtttgaa
       61 gagctatctg gcgactcatc cccgaaagaa caacagcgga atcaccgata acatgcgaga
      121 ggtggcaccg ctcgccgcag ccccagcgac cgaacgttag tgagcgagtg ggcgaggaag
      181 cggaagagaa ccggaaacca cattgagcac gtacgcactg atgcggggtg tcggggtgaa
      241 gccctgacca gatggcaaac ggaatatcgt cgcgggtgac ggtattacat ttgcacatcc
      301 tgtcccgatt tctgcggagt ttaacggtga acggacggca aaaaacgtgc aggagctgtt
      361 gaacgtgaaa acctcgaaat ctggcaaaaa tcacggctga cgaggcttgc agaggattaa
      421 gccagtatac actccgctat cgctacgtga ctggttcagg gctgcgcccc gaaaccccaa
      481 aaaaacaaaa accaagcgat cgtgcattag gaaacatcgt gctcttaaga taacaattag
      541 cggatgatgt aactcattgt ttttatttgt ggaaatatta gctaatagga aacacatgta
      601 tttccgcatg ataggaaata catgtgtttc ctattacttc tgaaagtagg aacgaaataa
      661 gcattgatat ggaaaataaa tttcctatta taggaacaac tgtttccggt tgataggaat
      721 cgctatgaat acccagaatc taatcgcaga tcatcgagaa aaacatgaca gagagaacct
      781 cggattcact caagtgtatc cactaggctg gaagcgactg atggctcttg ccaaaggaaa
      841 tcagggcgca tttgggttgt atactttttt ggcacaacat atcgatggtg cttgtggtgc
      901 ggttgtagcg gaccaagctt ttctggctgc tcagtttgat gtgactactc gaactgtgcg
      961 taattggatc tcatacctag aagagcaaaa agccatagtc aaaattcctg ttgcgggaag
     1021 agtttgcgcc tatgcactta acccacatga ggtctggaaa ggctatgaca acaacaagcc
     1081 tacagcagca ttcctgacaa agaccttagt cagcaaagac ggggaaatta agcgccgaat
     1141 catggccatg ttttcaaatt gagccagccg caggcagaca atcagggcaa cgtgttaacg
     1201 ttctgaccat gattgtctat cctgcattgc tccattgcca accataaagt cgctgtcgat
     1261 ccttgcaaat tcaacacgac caaatgcata ttcatcctga gttcaaaact caaacgtaag
     1321 tgataggtag ttattttcac cctccctgct caatttccgc agggagaatt tttatccttg
     1381 ctgtcacggt tccggcagcg ccttgagcgc aaccggaaac cgtctgggga gaaccccaga
     1441 cccccatcgc tgcaacaccg acggtctacg accaccaacc agctgaatag ctgccagtgc
     1501 cgtgcaaatg ggctttgcag ggggctgacg cggttcctgg cgacctgaaa gcccctggtt
     1561 aggggtatcg gcgcgcctga cctcctgaaa ccctctgtaa gcgtttttcg cgccttcca
//
