LOCUS       CP098989                1748 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB46-C15 plasmid unnamed5, complete
            sequence.
ACCESSION   CP098989
VERSION     CP098989.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488711
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB46-C15
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 115x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:22:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,876
            CDSs (total)                      :: 4,755
            Genes (coding)                    :: 4,475
            CDSs (with protein)               :: 4,475
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 280
            CDSs (without protein)            :: 280
            Pseudo Genes (ambiguous residues) :: 0 of 280
            Pseudo Genes (frameshifted)       :: 77 of 280
            Pseudo Genes (incomplete)         :: 194 of 280
            Pseudo Genes (internal stop)      :: 58 of 280
            Pseudo Genes (multiple problems)  :: 44 of 280
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB46-C15"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            473..1462
                     /locus_tag="NFL47_24370"
     CDS             473..1462
                     /locus_tag="NFL47_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WGB73060.1"
                     /translation="MKNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDSRWMFLTLTVRNCAIGDLGDTLSRMNTAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVKHARWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKIEVKKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          complement(1191..1380)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.859"
                     /coverage="99.0"
                     /evalue="3.7200000000000003e-85"
ORIGIN
        1 ttcgccgttc ctgaaatcgt caaacgctaa atcgtggggt cccgctggct tgccagtggg
       61 ttccgattta gtgattgacg agcgcacccg taaaacactg ccgacaggtg gttgtgatca
      121 cgcagtgagc gcaaccacct gataagcgtt tttggggttt gtggggtccg atttaacgtg
      181 gattttgggg gcaaaaaatc gcgcgcgttc cttgtattta tacttaaggg ataactctgg
      241 cgttaaaaat aagcctcagc ccttgtgccg caagggtttc agcgtgattt gcctgaaaat
      301 gactttgaag ttattctgtc tggagtggtc gcgttaaaag cgacccccgc aacgacggcc
      361 atcgttcggg ggcgtgacca cctgtaggaa ttctattttg cgacagacaa cacaggtgat
      421 acggcaatga taacacgtca agtgagccat cgcccagtat ggggcggtgc gcatgaaaaa
      481 tcctgaactc cagaacctca cggactattc cccctcagat gcgccctggg acgctcacag
      541 aagcgtttct gacgacgtgg gagggattta cctgcttgcg gccgaatatg agcgctacgg
      601 ggcgcgtatg gcctcctgcg gcggtttact gcgtttcggg tggtctaccc tcaaagaaac
      661 cggagaaacg cgcctgaggc tgcgtgaggc gcatttctgc cgtgtccggc actgtcctgt
      721 ctgccagtgg aggcgctccc tgatgtggca agcccgtttc taccagtccc tgccgcgtat
      781 cgtggcggat taccccgatt cccgctggat gttcctgacc ctgaccgtgc gcaactgtgc
      841 gatcggggat ctgggcgata cgctgagccg gatgaacacg gctttccagc ggctgaagga
      901 tcgcaaagag ttcagaccag ttcagggatg gatccgcacc acggaagtca cccgcggcag
      961 tgacggcagc gcacacccgc acttccacac actgatgatg gttccgcctg gcatgcttaa
     1021 cgggaagagt tacgtcaaac atgcgcgctg ggttgagctg tggcgggagt gccttcgggt
     1081 cagctacgat ccgaatgtcg atgtccgggc agtgaaaccc cggaagccga aggacggcga
     1141 atcgctggcc tgcgcgaccg ccgaactggt gcgcggcgcg gtggccgaga ccctgaaata
     1201 ttccacgaag ccggccgaca tggtcgccga tcctgaatgg ttcctcgaac tgacccgcca
     1261 gacgcacaag cgccgctttg tggcgaccgg cggcgcactc aaagacgtat tgaaactcga
     1321 tcaggaaacc gatgccgaca tggtgatcgg ggatgatatt tccgagggcg atgatgacgg
     1381 ttcccgcatc gcgttcgagt ggaaaatcga ggtcaaaaaa taccggcgtt cgccgacgaa
     1441 agataaaacc gaatcagagt aaaaacactg cgatgcagga tagggcaaac gccgcaaaat
     1501 gacgtcatcc gacatcattc cgccgcgttc gcccagtgac ctgcagtcgg ctctggggtg
     1561 tcggggctgc gccctgacct ggggttttgg attgcggcca tcgtgatggc ggtaatacaa
     1621 aacgcacggg acaagatgtg tttttggagt accgccgaca cgcgacggac agtccaaaat
     1681 atcttggtca gggccgcgcc ccgacaccct gccaacacct cacgccgcca gattaaaatc
     1741 tgccagcg
//
