LOCUS       CP098988                2524 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB46-C15 plasmid unnamed4, complete
            sequence.
ACCESSION   CP098988
VERSION     CP098988.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488711
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB46-C15
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 115x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:22:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,876
            CDSs (total)                      :: 4,755
            Genes (coding)                    :: 4,475
            CDSs (with protein)               :: 4,475
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 280
            CDSs (without protein)            :: 280
            Pseudo Genes (ambiguous residues) :: 0 of 280
            Pseudo Genes (frameshifted)       :: 77 of 280
            Pseudo Genes (incomplete)         :: 194 of 280
            Pseudo Genes (internal stop)      :: 58 of 280
            Pseudo Genes (multiple problems)  :: 44 of 280
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB46-C15"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2477..2524,1..66))
                     /locus_tag="NFL47_24355"
     CDS             complement(join(2477..2524,1..66))
                     /locus_tag="NFL47_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WGB73058.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAAIGN"
                     /name="replication initiation protein"
     gene            complement(714..908)
                     /locus_tag="NFL47_24360"
     CDS             complement(714..908)
                     /locus_tag="NFL47_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WGB73059.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1682..2161)
                     /locus_tag="NFL47_24365"
     CDS             complement(1682..2161)
                     /locus_tag="NFL47_24365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGB73057.1"
                     /translation="MRKTLLLCIPFFLLAGCAQTKPQMPDANYEKFAKVEVASDACLNS
                     NFITAQEAGQAHSNISLFLSSWTYDYVRYSNLLAQGHQDVKKIKITQEGCNLLRAKIYQ
                     DTIEAQRYEKQMEIAVQQKAIADQQALQSIQNMQSTSPKTTYCNQIGTQTICNSY"
                     /name="hypothetical protein"
     Typing          547..634
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          945..1043
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          1168..1423
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="89.0"
                     /evalue="1.21e-116"
     Typing          complement(2..531)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.727"
                     /coverage="98.0"
                     /evalue="8.250000000000003e-166"
ORIGIN
        1 gttggactca agacgatagt taccggacaa ggcgcagcag tcggactgaa cggggggttc
       61 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat
      121 gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag
      181 agcgcacgag ggagccacca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      241 gccacctctg acttgagcgt cgatttctgt gatgctcgtc aggggggcgg agcctatgga
      301 aaaacgccag cggcgtggcc ttttccaggt tctcgttttt ttgctcacat gtttcttttg
      361 gcgttatccc ctgattctgt ggataaccgt ataactgtct ttgagcgagc agataccgct
      421 cgccacagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aaagcgcctg
      481 tacgtgcatt ttctccttac gcatctgtgc ggcatttcac acccggcatg gtgcgctttt
      541 catacaatcc gcacagatgc cgcatgatta agccagtata cactccgcta tcgctacgtg
      601 actgggtcat ggctgcgccc cgacacccgc taaaacctcc tgacgcgccc tgacgggctt
      661 gtctgctgac gaaaacgctc gcggacaagc cgtgagcgcc tccgtcagct tcgttatgtt
      721 cctgtttcac tttcggtctc aaagcgaacc tggatgctgt tctggagttc ttccgcgagt
      781 tcgtgcagtc gttcgcacat ggccgcctgt tcgtcggcat ccagagcatc cagtttttca
      841 agcagtgtca ggctctggct tttgataaat cgcgccatat tcagcgcagt ttgttgctgt
      901 ttgttcacct tccgttgttc tccgtcagta ccgggtgtcg ggtggaccct gaccaggtgg
      961 taattgtaag acgttgcggg cgacggtatt acaattgcac atcctgtcct gtttttttag
     1021 gacgtatcat ggcacagcat atcaactgat aaataatccc tgtgcagtgc agccgctgtt
     1081 gcataaaatt tcgctctgcg tcgagtgttt cttaatcaat aattccgctg atactccgga
     1141 aaaacgcgag tctgttctca ttcctgaaat gcccacaccc cacgcaaaaa caattttttg
     1201 ctgatttttc tttataaata gagagttatg acaaattagt tcttcttgct ctctttgtga
     1261 tatttaaaaa agcggtgtcg gcgcggtgtt gtagctgcgc caacaccgct ttttaggggt
     1321 ggtactgact attttcataa aaaacatcat tttatattag gggtgctgct agcggcgcgg
     1381 tgtgtttttt tacaggacgc ccctgggggc gctgctaggg gtgtctgttc agatatgtag
     1441 caatgagtca gggattcacg aactgaaaat gtccgaaaat gtgtctcatg ggcaccgtat
     1501 gcgcccaaac cgatcgccat tcactttcat gcatagctat gcagtgagct gaaagcgatc
     1561 ctgacgcatt tttccggttt tcgcttggta aaacatctct ttttgcggtg tttgcggcag
     1621 aatcgtgttc agcgcgtttt agcggtgcgg gcaagggatg ttatggtaaa tcagtttttg
     1681 atcagtagct gttgcagatt gtttgagtgc caatttggtt acagtaagtt gttttaggtg
     1741 atgtgctttg catattttga atcgactgca acgcctgctg atctgctatg gctttttgtt
     1801 gtactgctat ctccatctgt ttttcatatc tctgtgcctc gatggtgtct tgatagattt
     1861 ttgcgcgcaa aaggttgcag ccttcctgtg ttatcttgat tttttttaca tcttgatgtc
     1921 cttgcgccaa gaggtttgag taacgaacgt agtcatatgt ccatgaactt aagaacaagg
     1981 atatgttgga atgtgcctga ccggcttcct gtgctgtaat gaagttggag tttaagcagg
     2041 cgtcagaggc aacctcaact tttgcgaatt tttcgtaatt agcatctggc atttgcggtt
     2101 ttgtttgagc gcaaccagca agtaaaaaaa acggaatgca aagtaataat gtttttctca
     2161 ttgagttttt tatccctttt tgacaagaaa aaactaatgc cccgtggttg gtatataaaa
     2221 aatcacgggg agcatagcga tcttcgtcct acttaataac ctaaatcttc ttgagatcat
     2281 ttatggcaga gcgtaatctc ttgctctgaa aacgaaaaaa ccacctgggg aggtggtttg
     2341 atcgaaggtt aggtcagttg gggaactgct taaccaggta actgtcttgt gcgagacgag
     2401 tcaccaaaac tgtccttcta gtgtagccgt tctcaggctc ccacttcaag aactccagat
     2461 aaatctctcg cacatctcag ttaccaatgg ctgctgccag tggcgctttt gcatgtcttt
     2521 ccgg
//
