LOCUS       CP098987                3897 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB46-C15 plasmid unnamed3, complete
            sequence.
ACCESSION   CP098987
VERSION     CP098987.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488711
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB46-C15
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 115x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:22:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,876
            CDSs (total)                      :: 4,755
            Genes (coding)                    :: 4,475
            CDSs (with protein)               :: 4,475
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 280
            CDSs (without protein)            :: 280
            Pseudo Genes (ambiguous residues) :: 0 of 280
            Pseudo Genes (frameshifted)       :: 77 of 280
            Pseudo Genes (incomplete)         :: 194 of 280
            Pseudo Genes (internal stop)      :: 58 of 280
            Pseudo Genes (multiple problems)  :: 44 of 280
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB46-C15"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(18..548)
                     /locus_tag="NFL47_24335"
     CDS             complement(18..548)
                     /locus_tag="NFL47_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGB73054.1"
                     /translation="MASTEEGLLTLLLSVEHLKNTAIEQQKAVADTLAVARETLRGYRD
                     ISDSVVKQVSDEVRKGVQKTDIAGLMGERMTQDFQRLEETVAKMRANAEALNKDIATIH
                     HNVVKEYNTLNKYSLKWFIGVTVSFLVMFSLLGWFTKTLIDRNYTATEVVYQKIVDIEK
                     KLDKPATGKSRKN"
                     /name="hypothetical protein"
     gene            complement(560..1513)
                     /locus_tag="NFL47_24340"
     CDS             complement(560..1513)
                     /locus_tag="NFL47_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WGB73055.1"
                     /translation="MATYHLNVKFGGKGQAGNHADYIERKGEYEKRKDLEYSEHGNMPG
                     WARDNPSHFWQAADQFERANGSTYREIEIALPRELTPDQRIELVQDFVRQVAGERHAYS
                     FAIHNPKASIDGGEQPHAHIMMSQRINDGMERSPEQYFRRYNAKNPERGGAKKDSGNLT
                     PTQQKEQLRTLRASWSALQNEHLARHGHRVRVDHRSLEERGIKRTPERHFGFAASSRLD
                     HAQRREILQNRAQPLEHFLEKAHSVRHQFDAQVQQKELVKTLLQDVARERGHALTARIN
                     KTGEVEFERAPTRTRSNTRGHGMGIRDNSRGYDYER"
                     /name="MobA/MobL family protein"
     gene            1693..2088
                     /locus_tag="NFL47_24345"
     CDS             1693..2088
                     /locus_tag="NFL47_24345"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGB73056.1"
                     /translation="MRISNIDWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNELQAQENARKQANQKRMEGKTQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELRYPKDNILQKLKAIRLDLETSPDHH"
                     /name="hypothetical protein"
     gene            complement(2451..3482)
                     /locus_tag="NFL47_24350"
     CDS             complement(2451..3482)
                     /locus_tag="NFL47_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WGB73053.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSKNQADKGKVG
                     GKNSGVVRWTANDDKRRRALDMYILGASTEDIAVAVGVSRWTVRRWMDSSVEWLLSKQI
                     IKI"
                     /name="replication initiation protein"
     Typing          complement(2707..3482)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.683"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(564..1513)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.685"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggacaagcg aagcgcgtca gtttttccgg ctcttccctg ttgctggttt gtccagtttc
       61 ttttcaatat caacaatttt ctgatacacg acctccgtcg cggtgtagtt cctgtcgatc
      121 aacgttttgg taaaccagcc aagcaacgag aacatgacca gaaaactgac cgtaacaccg
      181 ataaaccatt tcagactgta tttattgagc gtgttgtatt ctttaacaac attgtgatga
      241 attgtcgcaa tgtccttgtt aagcgcctcc gcgttggctc tcatctttgc cacagtctct
      301 tcaagacgct gaaaatcctg ggtcatgcgc tcacccatca gccccgcaat gtccgttttc
      361 tgtacccctt ttctgacttc atcgctaacc tgttttacaa cactgtcaga aatatcccga
      421 tacccccgca gtgtttccct tgcgaccgcc agcgtgtctg caaccgcttt ctgttgttct
      481 atcgcagtgt ttttcaaatg ctcaacggac aacaagagcg tgagcaaccc ctcttctgta
      541 ctcgccatac agtccccttt taacgttcgt agtcgtaccc gcgtgagttg tcacgaatgc
      601 ccatgccgtg ccctcgcgtg ttactccgcg tccgcgttgg cgcacgttca aattcaactt
      661 cacctgtttt atttattctt gccgtaagcg cgtgccctcg ctcccgtgcg acgtcctgta
      721 aaagcgtttt aactaactct ttttgctgaa cttgtgcatc aaattgatgg cgaaccgaat
      781 gcgctttctc tagaaaatgt tcgagcggct gcgctcggtt ttgcaggatt tcgcggcgct
      841 gcgcatgatc aagccgtgat gaggcggcaa atccgaaatg ccgttctggc gtgcgtttaa
      901 ttccgcgttc ttccagtgag cgatggtcaa cgcgcaccct gtgaccatga cgcgctaaat
      961 gctcgttctg taacgctgac cagctcgccc gtaacgtccg caattgctct ttttgctgag
     1021 tcggtgtcag attgcctgaa tctttttttg cgcctccccg ttcgggattc ttggcgttat
     1081 aacgccggaa atactgttcc ggcgaacgtt ccataccatc gttaatacgc tggctcatca
     1141 tgatatgcgc atgaggctgt tcccctccgt caatgctcgc ttttggattg tgaatggcaa
     1201 agctataggc gtgccgttct ccagctacct gacgcacaaa atcctgcacc agttcaatgc
     1261 gctgatccgg tgtcagttca cgcggcaagg cgatttcgat ctcccgataa gtcgaaccat
     1321 tcgcacgttc aaactgatca gccgcttgcc agaaatggga gggattatct cgcgcccagc
     1381 ctggcatatt cccgtgctct gagtattcca gatcttttcg tttctcgtac tcgcctttgc
     1441 gttcgatgta atcggcatgg tttccggcct gtcccttgcc gccaaatttt acattgaggt
     1501 gataggttgc cacggtgtta gccctccgca ggagcgcaca tgtttctgag ccaacggcga
     1561 agaaacatat aagtgcgctt cctgctaatt ttacgaatta gcacggaaga tacccccctg
     1621 cacccaccca ccgcaagcga tatactgcac aaccccaaca caccgcaaaa aacaccaggg
     1681 agcatcccaa acatgcgcat cagcaacatc gactggttga agaaacgaat cggctttatc
     1741 aggaagctcg gagaacagac cgcacgacag aggcagatca ttgacctgct cgataacgaa
     1801 gccggactaa cagaacagga gcgcaaacta ctccacgtcc tggcaaccgc agaaaaaaac
     1861 gagctacagg cacaggagaa cgctcgcaaa caggccaacc aaaaacggat ggaggggaaa
     1921 acacaacgac gggaacgcaa tcaccgcctg tttcttgcgg cggggttact gatcgaggcc
     1981 ggactagtgg acacaaaaac aggcgaactg agatacccaa aagacaatat attacagaaa
     2041 cttaaggcaa tcaggctcga tcttgaaaca agcccagatc atcattaatt ccgctgcgtg
     2101 gtggtgcgtt tgcctgccgg tgttcaatgt tttcgatttc gcgacgcgct acgcgcggat
     2161 ctggcatagg tgcgtaagtg ctcaatgtga cttccgatta tcttccgctt cctcgcccac
     2221 tcgcgagcct gacggctcgg tcgctagggc tgcggcgagc ggagccacca ctcgcttggt
     2281 ttgcagggtt tcggtatttg gtttgggggt gtgggagcgg gtcggagacg ccgcgcaaaa
     2341 aaacaccaaa cccccgttca ggcgctgtta tctgatatag gcttgcttcg tgcacccaaa
     2401 attaacaaaa aactaacaaa acaggggggg gtatgtattc tttttgttaa ttaaattttg
     2461 attatttgtt tactcagtag ccattctaca gagctatcca tccagcgtcg aacagtccaa
     2521 cggctaacac caaccgccac tgcaatatct tctgttgacg ctcccaagat gtacatatca
     2581 agcgccctac gacgtttatc atcgttagcc gtccaccgga caacaccgct gtttttcccg
     2641 ccaactttcc ctttgtccgc ctgatttttt gacgaattgg ccttcccgcc cttgcgacct
     2701 cgtgcggcct gaatttctgg cgtgtgcgta tcagccacat actgtgcgaa agtttccggc
     2761 gtgaagttcc tgtgcgtgta tttcgcaatg ctcttgccaa tagcccgaca ttcagccggt
     2821 gaaagcggaa cgggaagcga tgcgttatac atttcgacac gctggatcac ggcatcaagc
     2881 cactgtgaga atgctggcca gccctgacga atcgccctgt aagcccattt gcgcgtcatt
     2941 tcgaacaggt ggcagtttcg ccccatccca taatgtttat cgatgctacg acgcgctgag
     3001 gcgctcaaat cgagataatc agccagttca tcgagggtat aggcttcctc gcgccactcc
     3061 atcaccagcc attccaggtg gaacggattt ttgcaaatca agccgctgta gttcacatcc
     3121 gcacaaagtt tctcacacag cgaacgttca accgctgcgg catatttcag cgccttaacc
     3181 gacgcatcag gcgcggttct cacggcgata ttgagtgcat agagcaggtg agcgtgtccg
     3241 ttcacaggat ttttaacggt gatgttcggc gcgggggcgt tccggtcgct ccagtcaatc
     3301 gctgccccct cgcggtcaac gtcaaaaacc agccagaact gcgcatgtgg ctggttctgc
     3361 tggatgtacc gcgcaagaag cgcacgccct ttgccggaga tgcgaaggcc gaaagcgaga
     3421 tcatccgtgt gatagggacg gtgaggtaaa ttttcttcga agtattgaag cgcggcgctc
     3481 atgtgttagg attcctccgt gtgttacgac ccacacgccc ctgtatcttc gtgacagtct
     3541 tggcggatag acacgaattt caggggcgtt tgtttttcag tcgcttacac tacccccaac
     3601 gacaaaacct gtcgagtctt cccgcaaacc cgatccattt accataactc ctgttgtacg
     3661 taccccttaa aacgccctca atcgctttct ggcgcaaaaa acgcaaaaag acaaggaaac
     3721 accaggcaaa gccagaaaaa cgcgtcagaa tcgttttcat gagcctgcat aactattcac
     3781 gaaagcaatg cgcgatctgt ttcgagcgtt cagaagggcg aacggggcaa aacagcaaac
     3841 tttctcatca gaaaacgcct cacgggacgc ggaatttttt cgcgtcccgt gaggcgt
//
