LOCUS       CP098982                2724 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB46-C16 plasmid unnamed3, complete
            sequence.
ACCESSION   CP098982
VERSION     CP098982.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488712
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB46-C16
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 178x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:00:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,570
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 63 of 224
            Pseudo Genes (incomplete)         :: 153 of 224
            Pseudo Genes (internal stop)      :: 47 of 224
            Pseudo Genes (multiple problems)  :: 34 of 224
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB46-C16"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(491..1255)
                     /locus_tag="NFL48_23430"
     CDS             complement(491..1255)
                     /locus_tag="NFL48_23430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGB77407.1"
                     /translation="MLGKIWCSLITVTFTLLIGCVPQPKPVDKFDINKLLNVTDSETTT
                     DLAIPSPAQQDPIWQTSLLTGRQLKEGIDAGKIKRGDVFRAKIDLKYTIVNKSQLPPAL
                     DVFRSVLIGDPSAWNDLIAIDISDSKHTKTGKYVAQNAFGARVKVDEVKSERYLLALDA
                     ANTLSTTAIKMGVEGCAVTEDSLNSFVLEYVAELDESTYQQEKGIIPAEYSSPTDIRIN
                     NYYFKGEVRAARLIDSTTGKPYPCKLSFIIRY"
                     /name="hypothetical protein"
     gene            2047..2241
                     /locus_tag="NFL48_23435"
     CDS             2047..2241
                     /locus_tag="NFL48_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WGB77406.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(1533..1788)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="89.0"
                     /evalue="1.21e-116"
     Typing          complement(1913..2011)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          complement(2319..2502)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.185"
                     /coverage="86.0"
                     /evalue="1.26e-45"
     Typing          2..444
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.087"
                     /coverage="89.0"
                     /evalue="1.41e-108"
     Typing          2590..2724
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.928"
                     /coverage="89.0"
                     /evalue="2.09e-41"
ORIGIN
        1 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
       61 cgcgtttatc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
      121 gactgtatgc acgaaccccc cgttcagtcc gactaccacg ctcttttcgg taactatcgt
      181 cttgagtcca acccgaaaag gcacgacaaa tcgccattgg cggtagccat tggtaactga
      241 tttagaggag gagagtcttg aagttatgcg ccagttaagg ctaaactgaa aggacaagtt
      301 ttggtgactg cgctcctcca agccagttac cgcggttcaa agagttggta gctcagagaa
      361 ccttcgaaaa accgccctgc aaggcggttt tttcgtttac agagcaagag attacgcgta
      421 gataaaaagg atctcaagaa gatcctttta taaaactgag tcaacatatc aatctcggca
      481 tagtgtatct ttaatacctt atgataaaac ttaacttaca tgggtatggt ttcccggttg
      541 tcgagtcaat caatcgagca gctctaactt cccctttgaa atagtaattg ttaatacgaa
      601 tatctgtagg gctactgtat tcagcaggaa taattccttt ctcctgctgg tatgtgcttt
      661 catctaattc agcaacgtac tcgagcacaa aggaattaag agagtcctct gttaccgcac
      721 atccctcaac tcccattttg atcgctgtag tcgatagtgt attcgccgca tcaagcgcca
      781 ataagtaacg ttctgattta acctcatcta ccttaactct tgcgccaaaa gcattctggg
      841 ctacatattt ccccgttttg gtatgcttgg agtcagatat atcaatagca atgagatcat
      901 tccacgctga tgggtcccct attaacacac ttcggaaaac gtcaagtgca ggtggtagtt
      961 gagacttatt gactatcgta tacttcaggt cgatttttgc ccgaaacaca tcaccacgct
     1021 ttatttttcc tgcgtcaatc ccttccttca attgccgccc tgtcaacaag gaagtctgcc
     1081 aaatagggtc ttgttgagcg ggtgatggga tcgcaagatc tgtagttgtt tcactatctg
     1141 tgacgttaag aagtttgttt atatcaaatt tatcaaccgg tttcggttgt ggaacacatc
     1201 caatcaataa tgtgaaagta actgtaatca gtgaacacca tatttttccc aacatgctag
     1261 ctccatagct ccataaaaaa ggaattcaac ataacatccg ttgcgcgcac cgctaaaacg
     1321 cgctgaacgc gattctgccg cgaacaccgc aaaaagagat gttctccccg gggtaaaccg
     1381 gaaaaatgcg tcaggatcgc tttcagctca ctgcatagct atgcatgaaa gtaaatgacg
     1441 atcggtttgg gcgcatacag tgcccatgag acacattttc ggacattttc agttcgtgaa
     1501 tccctgactc attgctacat atctgaacag acacccctag cagcgacccc aggggtgtcc
     1561 tgtaaaaaaa cacaccgcgc cgctagcagc acccctaata taagatgatg ttttttatga
     1621 aaatagtcag taccacccct aaaaagcggt gttggcgcag ctacaacacc gcgccgacac
     1681 cgctttttta aatatcacaa agagagcaag aagaactaat ttgtcataac tctctattta
     1741 taaagaaaaa tcagcaaaaa cttgtttttg cgtggggtgt gggcatttca ggcatgagaa
     1801 cagactcgcg tttttccgga gtatcagcgg aattattgat taagaaacac tcgacgcaga
     1861 gcgaaatttt atgcaacagc ggctgcactg cacagggatt atttatcagt tgatatgctg
     1921 tgccatgata cgtcctaaag aaacaggaca ggatgtgcaa ttgtaatacc gtcgcccgca
     1981 acgtcttaca attaccacct gctcagggtc cccccgacac ccggaactga cggagaacaa
     2041 cggaaggtga acaaacaaca acaaactgcg ctgaatatgg cgcgatttat caaaagccag
     2101 agcctgacac tgcttgaaaa actggatgct ctggatgccg acgaacaggc ggccatgtgc
     2161 gaacgactgc acgaactcgc agaagaactc cagaacagca tccagattcg ctttgaggcc
     2221 gaaagtgaaa caggaacata acgaagctga cggaggcggt cacggctttc ccgtgggcgt
     2281 tttcgtcagc agacaagccc gtcagggcgc gtcagggggt tttagcgggt gtcggggcgc
     2341 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggctaaatat gttacggcgg
     2401 ggtaacactt aataaaagtg cttcatgtga caggggaaaa tgcagctaca gggcgtaagg
     2461 gagaaaatgg gagttacggt gatatgacgc ctcctcgctc actcggtcgc tgccgctcct
     2521 gccgttcggc tgcggcgggc gttacctgca tacggataag agcaaaggca tacggcatga
     2581 caagcagggc aaaaaaagga tacgccgctg ccgtttttcc ataggctccg cccccctgac
     2641 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg acttaaagat
     2701 accaggcgtt tccccctggt ggct
//
