LOCUS       CP098977                5874 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB46-C17 plasmid unnamed2, complete
            sequence.
ACCESSION   CP098977
VERSION     CP098977.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488713
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB46-C17
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 125x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:00:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,185
            CDSs (total)                      :: 5,066
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 264
            CDSs (without protein)            :: 264
            Pseudo Genes (ambiguous residues) :: 0 of 264
            Pseudo Genes (frameshifted)       :: 78 of 264
            Pseudo Genes (incomplete)         :: 199 of 264
            Pseudo Genes (internal stop)      :: 58 of 264
            Pseudo Genes (multiple problems)  :: 58 of 264
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB46-C17"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(5714..5874,1..16))
                     /locus_tag="NFL49_25890"
     CDS             complement(join(5714..5874,1..16))
                     /locus_tag="NFL49_25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WGB82207.1"
                     /translation="MQRLNEWENAFVELQKMFSLTQRDNAMLNGRVMQLSQQVQHLSER
                     TERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(36..83)
                     /locus_tag="NFL49_25895"
                     /pseudo=""
     CDS             complement(36..83)
                     /locus_tag="NFL49_25895"
                     /inference="COORDINATES: protein motif:HMM:NF016769.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(80..610)
                     /locus_tag="NFL49_25900"
     CDS             complement(80..610)
                     /locus_tag="NFL49_25900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WGB82208.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(648..1079)
                     /locus_tag="NFL49_25905"
                     /pseudo=""
     CDS             complement(648..1079)
                     /locus_tag="NFL49_25905"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1288..1611)
                     /locus_tag="NFL49_25910"
     CDS             complement(1288..1611)
                     /locus_tag="NFL49_25910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WGB82210.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAAWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALIAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1677..1868
                     /locus_tag="NFL49_25915"
     CDS             1677..1868
                     /locus_tag="NFL49_25915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WGB82209.1"
                     /translation="MTKQEKTALNMVRFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3074..3940)
                     /locus_tag="NFL49_25920"
     CDS             complement(3074..3940)
                     /locus_tag="NFL49_25920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WGB82205.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(3942..4994)
                     /locus_tag="NFL49_25925"
     CDS             complement(3942..4994)
                     /locus_tag="NFL49_25925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGB82206.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGNGGYSHPEGISTTWHSESKLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIDNSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGVPVDDASMFFNSFQPRKFE
                     NTPLYEKEDD"
                     /name="hypothetical protein"
     Typing          complement(1963..2044)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          complement(2501..2688)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(4..1298)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgttcagccg ctgcatatag tcctgttgca gttgctctaa tgcgttcagc aaatgtcttt
       61 ccagctccgt catgctctgt cactcctgca ccagtctcac tgcgttcagt ttgccgttgt
      121 ccttcgtcca gttcgtttcg gctttcatcc ccttcggcaa caccagaaaa cgtccgttgc
      181 tgtcctcgtg ataacgcacg ccccatgtct tcgcgttcag cttctccaga ctctctttct
      241 gctcgcggag aatattcata ttcgcgttta tcttcatgcc ctggtaccac agcagactcc
      301 cggtcgtggc aaacatcagt actgagacga taatcagcca cagccagcgt ttcacgctca
      361 ttttcagtac ggttttccag tgcgcgtcca tcgtatcgac ggtcgttttc tgatacctct
      421 ccagcagttc tttcagggct gttgtatggt cgttgatagc gttcctgatt aaagattcgt
      481 ttgaggtcag ttccctgttt acagattctt cgagcttcct gaactcgtcg ttcagcattt
      541 tctctgtaga ttcggctctc tctttcagct ttttctcgaa ctccgcgccc gtctgtaaaa
      601 gattgctcat aaaacgctcc tttcagtctg atattcctcc cgccgttcgg gtctgcaatg
      661 ctgatactgc ttcgcgtcac cctgaccact tcaagccccg cctctgtgag cgcctgaatc
      721 acatcctgac ggccttttat ctctccggca tggtaaagcg cgtctatccc gcgtgtgacg
      781 ccctcagcaa gcgcctgttt cgtttccggc aggttatcag gaagggtcag cgtccggcgg
      841 ttctccgggg cgttggggtc atgcagaccg taatggtgat tcaccagcgt ctgccaggca
      901 tcaattctcg gcctgtctgc ccggtcgtag tacggctgga ggcgttttcc ggtctgtagc
      961 tccatgttcg gaatgacaaa attcagctca agccgtccct tgtcctggtg ctccacccac
     1021 aggatgctgt actgattttt ctcgagaccg ggcatcagta cacgctcaaa gctcgccatc
     1081 actttttcac gccctccagg cggcagctcc ttctccgcga acgacagaac acctgacgtg
     1141 tatttcttcg caaatggcgt ggcatcgatg agttcccgga cctcttccgg attcccctga
     1201 agcaccgttg ccccttcccg gttacgctcc cttcccagca ggtaatcaac cggaccactg
     1261 ccaccacctt ttcccctggc atgaaactta actatcatcc cgcgccccct gttccctgac
     1321 cgccagacgc agccggcgca gctcatcccc gatggctatc agtgcggcca ccacctgaac
     1381 ccggtcaccg gaagaccact gcccgctgtt caccttcctc gctgtctggt taaggttatt
     1441 cccgatagcg gccagctgac gcagtaacgg cggtgccagt gtcggcagct tcccggaacg
     1501 ggcaacaggc tcacccaggc agacccgccg catccatgcc gccagttgtt taccctcaca
     1561 gcgttccagt aaacgggcat gctcatcatc agtcacccgt atcgtgagca tcctctcgcg
     1621 tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg catccagtga
     1681 ctaaacagga aaaaaccgcc cttaacatgg tccgctttat cagaagccag acattaacgc
     1741 tgctggagaa gctcaacgaa ctggacgcgg atgaacaggc agacatctgt gaatcgcttc
     1801 acgaccacgc cgatgagctt taccgcagtt gcctcgcacg cttcggggat gacggtgaaa
     1861 accgctgaca catgcagctc ccggagacgg tcacagcttg tctgtgagcg gatgccggga
     1921 gcagacaagc ccgccagggc gcgtcaggcc gttgaccttt ggggtgtcgg ggcgaagccc
     1981 tgacccagtc acgtagcgat agcggagtgt atactggctt aatcattcgg catcagtgcg
     2041 gattgtatga aaagtgcgcc atgtcgggtg tgaaatgccg cacagatgcg taaggagaaa
     2101 atgccagttc aggcgctctt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
     2161 gctgcggcga gcggactctc ctttctcaaa aacgctaacc ctgttatcca cagaacccgg
     2221 ggataaacca ggaaagaaca tgtgcgcaaa aaacgaaacc ccggaaaagg ccgcgccgga
     2281 ggcgcttttt ccataggctc cgcccccctg acggacttca caaaacagac actttttcgg
     2341 tgcatctgtg ggagccttgc ggctcaacca tgaaatccgc cagcggtggc gaaacccgac
     2401 gtggcttaaa gatcatcacc gtttccggtg aatcgctccc tcctgcgccc ccacgttccc
     2461 gccatatcga ttaccggata ccttttccgc ctttctccct tacgggacac gtggcgcttt
     2521 ctcatagctc acgtcttctg gtatctcggg gcgacttagg cagttcgctc cgggctgggc
     2581 tgtatgcagg aaccccccgt tcagcccgac tgctgcgcct tctccggtaa ctgttctctt
     2641 gggtccaacc cggaaaagac accacaaagc gccactggca gcagccattg gtaaaagtca
     2701 tgtgcgagat atttagagag tacttgaagt ggctgccgga gtgcggctac actagaagaa
     2761 caattttggt gactcgtctc gcataagaca gttacctggt taagcagtct gcaagctgac
     2821 ttaaccttcg atcaaaccgc cctgcaaggc ggttttttcg tttacaggca gcagattacg
     2881 cgcagaaaaa aaggatctca agaggatcct ttgatctttt tgttacaaac gtctctattt
     2941 gactcccctg agagcgtttt agcgcgcctc tgccccccct tggttacatt ccgacaccag
     3001 aaacgctccc gttttttacg tgcatttggc acggtatcga tggaggccgg ggggggcaat
     3061 cctgaacaga atgttactcc tctgagtatt ttctgttaag aaaatataca cttagcacag
     3121 ttacaataaa tcctaccccc tgtaagaatg tgaattgttc atccaaaaac aaccatgaca
     3181 aaaataatgc aactagcgga ataaagtaca acattatccc tgccaaatga actgggagcc
     3241 aacgcaagga gttcaagtaa aaacaaaaag ctaaaaaata ttgtaatatt ccagatgaaa
     3301 aaacaataat cacggattcc aacggaagat aaattgagga tttagatata aaaataaaga
     3361 gcgatgacag tataagggag aatgtcaact gtccgctcaa taatgtgata ggctcaaacg
     3421 atttaataag gcgagacgat ataacaacat aaaatgctgc cgataacaca ccggcaacaa
     3481 caagacaata tccaacaata ttattcgatc cattactgat gtctggcaag gctaccaaaa
     3541 tactgccaac aattgcacct ataaggaaca atataaaata ttgcggggaa ttaattttaa
     3601 ctgagcaaat aagaacagac aacaccaata taaacactga ttcaagtgaa aaaataattg
     3661 atgcgcttcc tgctgaaata aaataaagtc cgtagagtgt aaaagtatac gctaagaatg
     3721 gttcaaaaac gcctgtcgca taagcaagta tcgcttttga gtttaatggg agtttttttc
     3781 tttttataaa aagaactatc caagatagta ttactgcact acaaatctgc cagaaaaaca
     3841 cccctccggc atctagttct tcaactcctt ttttgctcat tacgatagct gccccccatg
     3901 agaacgctgc taaaaaagca aaaagcattc ctttaatcat attaatcatc ctctttctca
     3961 taaagggggg tgttttcaaa tttccttggt tgaaaggagt taaaaaacat agatgcatca
     4021 tctacaggaa caccaataaa ctctccataa taaggatcat ctataggaaa ctcttctaat
     4081 gaaacataaa acaatacatt ccagttgaag atcattggat cactattatc tatttcatgg
     4141 agcaatcttt catccttagt taattctcct ggaattaata catcagtatg ataaaaaccg
     4201 ctgtaataag aactattacc ggtgtgcact gggcatccaa aacgatccaa tttagattct
     4261 gaatgccatg ttgtagaaat cccttccgga tgtgaatacc caccgttacc aacagactga
     4321 atagtcatgt tacgaatatc cgctaacgct tgttcttgat ctttatagaa acctttttct
     4381 atcgccagcc tcataaaatg ccccggggca tttacagaaa taagtattcc ccccggtatt
     4441 cttctatcat gatttcttgt tctatgagca gaagagtaaa aaacattaac accagcctta
     4501 tatattttta gcccatcttt atcctgaaat ggtacgcttt cagtataaat aacatcctct
     4561 tttattggat agcactctgg catatgcagc gaacaaatat atttttgtat gtccactaac
     4621 tcttctgatg gacgagcaaa tgcaagttgt ggatgaataa aataaatgag atgcgcactg
     4681 acctctcctc ttcttgcgcg ttctttaaaa cgaactcttt cattacgcaa aaaatcaaac
     4741 aaatgaccat catcattata tagttggggg ctatttacaa tagataaatg gaaatcaagc
     4801 cctttaattt tcttgcttgc caatttacca aatacacagg gctgattgcc tgacacccat
     4861 tttcttagta aatcttcaat tttatcttta gaagtttcgt taaagataaa tgaatgaata
     4921 ttatcaatat cttttccaaa tgaaaaactg gatgataatt gtggtagttt atttatacgt
     4981 tgttgaagat tcatgaaagc tcccacaaga cacctcatat attttttata tactgaaact
     5041 acattatatt tattaccgac gtaaaacgaa ataatttatt atcgttaccg atattttaaa
     5101 aaatcattat ttgcacagtg ttcgagttgt tagtatcact ccttcctgaa aatccgctcc
     5161 atttaccata acgtcgcatt gtacgcaccg ccaaaacgcg ttcggtgcga tcacggcagc
     5221 agacaggtaa aaatggcaac aaaccacccg aaaaactgcc gcgatcgcac ccgataaatt
     5281 ttaaccacat gcatagctat gcagccatgt gaatcacgca ggaattgcca gccagagact
     5341 gctgaaacgg atttttttca tactttcgga aaggcagaag acggaaccgg agcggaaaaa
     5401 cagaaattca tgggaatgag cgcgatctgg caataacggc aaaacacagc aacaaagaca
     5461 caccagaatc gcgcccgcat gcgttttaac gcgttcctgt gttttcagca agctctgact
     5521 gaagcgcgtt cccggtgccc ccgaaccaac cattatccca aaaaatgcac cggaaacgcg
     5581 ctgccaacat tttagcctgt ctctggttac catcccagcg aagggccatc cagtgtccgt
     5641 tcctgaattt ccggctggcg ctcccgctcc agggataacg catattcgcg ctcctgtatc
     5701 agccgctcat cccttatctc cagttctcgc tgtataactg gctcaagcgt tctgtccgct
     5761 cgctcaagtg ctgcacctgc tgactcaact gcatgactcg cccgttcagc atcgcgttgt
     5821 cccgttgcgt aagcgaaaac attttctgca attccacgaa ggcgttctcc catt
//
