LOCUS       CP098974                3861 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB46-C19 plasmid unnamed3, complete
            sequence.
ACCESSION   CP098974
VERSION     CP098974.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488714
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB46-C19
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 172x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:17:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,747
            CDSs (total)                      :: 4,635
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 249
            CDSs (without protein)            :: 249
            Pseudo Genes (ambiguous residues) :: 0 of 249
            Pseudo Genes (frameshifted)       :: 51 of 249
            Pseudo Genes (incomplete)         :: 187 of 249
            Pseudo Genes (internal stop)      :: 43 of 249
            Pseudo Genes (multiple problems)  :: 29 of 249
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB46-C19"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3365..3861,1..430)
                     /locus_tag="NFL50_23715"
     CDS             join(3365..3861,1..430)
                     /locus_tag="NFL50_23715"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HindIII family type II restriction endonuclease"
                     /protein_id="WGB86594.1"
                     /translation="MELGDFKFINKDAVIRRRYWIDEIVKLSGHFVNDSSRVENEIIDE
                     VKKSGNQALLDHLRLCTAIPESYGHDSSEEKLYSKYTDALISECFKYLGLNSIVLTERA
                     DAADVEVVCDNYSFVADAKVFRLSRTAKNQKDFKVQAMDGWRNTKDFAMVVCPIYQLPT
                     KSSQIYQQAILRNVCIFTYTHLAVLIRYSSIATTEDSKKILGDIFKSVSLLNPSKDSVQ
                     YWVNINRTMLNYDDRIAALWADEKLATTEGIAVSKKMAIEFLSSERTRMLMMTKDEAVN
                     ALIKMHKIDSRIDQINKVTDNNILSLK"
                     /name="HindIII family type II restriction endonuclease"
     gene            complement(431..1348)
                     /locus_tag="NFL50_23720"
     CDS             complement(431..1348)
                     /locus_tag="NFL50_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WGB86595.1"
                     /translation="MLKEIPLLDEIEHNSITNGDSIELIKKVQTQSAHLILSDIPYGIG
                     ADDWDVLHKNSNNAYLGSSPAQKSAGAIFKKRGKPINGWSDADRKIPLEYQQWCEEWAS
                     EWYRTLKPGASAIIFAGRRFSHRCICAMENAGFNLRDIIAWMRTKAPHRAQRLSCVYER
                     RGDKYNTEKWEGWRVGNLQPTFEPILWFSKPYKIGGTIADNVLLHGVGAYNQDAFVARN
                     GKPENVIHAGFASKESGLHPTQKPISLMKTLIELTTQEGQLVIDPFSGSGSTLVAAKEL
                     GRSYIGFELNPAYVEISKKRLKDK"
                     /name="site-specific DNA-methyltransferase"
     gene            complement(1570..1728)
                     /locus_tag="NFL50_23725"
     CDS             complement(1570..1728)
                     /locus_tag="NFL50_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WGB86596.1"
                     /translation="MHEPPVQPDCCACSGNYRLESNPERHDKTPLAAAIGIWFTEQRGA
                     YKHTQSS"
                     /name="replication initiation protein"
     gene            2299..2448
                     /locus_tag="NFL50_23730"
     CDS             2299..2448
                     /locus_tag="NFL50_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGB86592.1"
                     /translation="MPSEYDEQRGSTDTQLPGEYDLFDLMAKVTEEILHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            2688..3212
                     /locus_tag="NFL50_23735"
     CDS             2688..3212
                     /locus_tag="NFL50_23735"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGB86593.1"
                     /translation="MTKKSVPHCIVHACMNGLFFLTTELHRSFSSYITFTTLLQHSFGL
                     YITFTTLFHHFYIIYTSLLHHSFSSYVTFTSVPSSVPFVHPPPFLNVLKTVFPAVPATP
                     HGHAINCVPVHRFRVRRTSETGLTPAEWREFPHYALARILMHAVKTEPARFWRVFGWFV
                     ACFTGFPSETP"
                     /name="hypothetical protein"
     Typing          2091..2264
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.286"
                     /coverage="100.0"
                     /evalue="2.58e-81"
     Typing          complement(1621..2124)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.248"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 tcaaatatat cagcaagcca tattgcgcaa tgtttgtatt tttacatata cacatctggc
       61 tgtactgata agatattcaa gtattgcaac aactgaagat agcaaaaaaa ttcttggtga
      121 tatatttaaa tctgtatctt tacttaaccc cagcaaagat agtgttcaat attgggttaa
      181 tataaataga acaatgctaa actatgatga caggatagct gccctttggg ctgatgaaaa
      241 gctagccaca actgagggca ttgccgtttc aaaaaaaatg gctatcgaat tcttatcttc
      301 tgaacgtaca agaatgttaa tgatgaccaa agatgaggct gtcaacgcgc tgataaaaat
      361 gcataaaata gatagcagga tagaccaaat aaataaagtg acagataata acatactgtc
      421 acttaaataa ttatttgtct tttaatcttt ttttgcttat ttctacatat gctgggttaa
      481 gttcaaagcc tatatagctt ctacccagct cttttgcggc aacaagagtt gaaccgctac
      541 cactaaatgg atctattact agctgacctt cttgagtggt tagttctatt agggttttca
      601 tcagagaaat aggtttctgc gttgggtgta atccgctttc cttgctcgca aaacctgcat
      661 gtattacatt ttccggtttt ccatttctcg ctacgaacgc gtcttggtta tatgcaccca
      721 cgccatgcaa tagaacgtta tcagcaatgg tacctcctat tttataaggt tttgaaaacc
      781 atagtatagg ctcaaatgtt ggttgtaagt tacccactct ccagccttcc catttttctg
      841 tgttatattt gtcgccacgc ctttcatata cgcagcttag cctttgtgcg cgatgaggtg
      901 cttttgttct catccaggcg attatatctc tgaggttaaa tccagcattt tccattgcac
      961 aaatacatct atgtgaaaat cttctaccag caaaaataat ggcactagct cctggtttta
     1021 aagttcgata ccactcactc gcccactctt cacaccattg ctgatactct aaaggtattt
     1081 ttctatctgc atctgaccag ccatttatag gctttcctct ttttttgaaa atggctccag
     1141 ccgatttttg tgcagggctt gaacctaggt aggcattgtt tgagttttta tgtagtacat
     1201 cccaatcatc agcacctatt ccgtaaggga tgtcactcag tatcaagtgt gcggattgag
     1261 tttgtacttt ttttattagc tcgattgagt caccatttgt tatggagttg tgttctatct
     1321 catcaagcaa tggaatttct tttaacattc aacaccttac ccgcgattat ttcacttcat
     1381 tataactgtt aaagtatctt gacgcaggat aacctttcgt cgtagccttt tgaaaagaaa
     1441 aaaccgcctg gggaggcggt tttttcaggg ttcagttagt tggggaactt ctgaaccgtg
     1501 gtaactggct taaacggagc gcagtgacca aatctgtcct tccagtgtag ccgtagtcag
     1561 gtcaacactt caagaactct gtgtatgctt gtacgcgcct ctctgctccg taaaccagat
     1621 accaatggct gctgccagtg gcgttttgtc gtgtctttcc gggttggact caagtcgata
     1681 gttaccggaa caggcgcagc agtcgggctg aacggggggt tcgtgcatac agtccagctt
     1741 ggagcgaact gccttcccgg aatcgagtgt caggcgtgga atgagataaa cgcggccata
     1801 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccgc
     1861 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
     1921 gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg
     1981 ccctgtcgct tccctgttaa gtctcttcct ggcatcttcc aggaaatctc cgccccgttc
     2041 gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
     2101 agcggaatat atcctgtatc acatattctg ctgacgcacc ggtgcagcct tttttctcct
     2161 gccacatgaa gcacttcact gacatcctca tcagtgccaa catagtaagc cagtatacac
     2221 tccgctagcg ctacgtgact ggttcagggc tgcgccccga aacccgctaa caacctctct
     2281 gctactggat atcaccccat gccatctgaa tatgatgaac aacggggttc gactgatacg
     2341 cagcttcccg gcgaatatga cctttttgat ttaatggcga aggttactga agagatcctg
     2401 catgcccctg tggattctgg gccgcctgtc ggccgggaag cgttatagct ttttcgcttg
     2461 cccgcgtacg tggtaatacg ccggtattac cttttatttc tttattttca tgtaattaca
     2521 gtgggttttt aatacctggg tattactcgt gaaaacgaaa cattcctttt ttttctttta
     2581 attcatgcag ttgcgatgaa aatcgcggct gcgttaggtg tatgaccatt ttgaggttga
     2641 aaaataatca accatggagg gtgatttacg caggttaagg ggagatcgtg acaaaaaaaa
     2701 gcgttcctca ctgtatcgtt catgcctgca tgaacgggtt attcttcctg actacagaat
     2761 tacatcgctc cttcagttca tacatcactt ttacaacact tttacaacac tccttcggtc
     2821 tgtacatcac ttttacaaca ctttttcatc acttttacat catttataca tcacttttac
     2881 atcattcctt cagctcatac gtcactttta cgtcagttcc atcctctgtg ccatttgtac
     2941 atccgccccc cttcctgaac gtgctgaaaa cggtttttcc ggctgtgccg gcaacgcctc
     3001 acggccacgc aataaattgc gtccccgttc accgttttcg ggtaagaaga acatcggaaa
     3061 caggacttac cccggctgaa tggcgtgaat ttccgcacta tgcacttgcg cgcatactca
     3121 tgcatgccgt aaaaacagag cctgcgcgtt tctggcgggt tttcgggtgg tttgttgcct
     3181 gttttaccgg tttcccgtca gaaacgccct gagggcctct caggcggtgc acgcaacgga
     3241 tgttatggta aatcaagtga atgtctagcc acataactga tggaggtatt attatccagg
     3301 taaaaaagtt gttatgatcg agaaattata ttgagttatt gaacaataat attgggaatt
     3361 aaaaatggaa ttaggtgatt tcaaattcat taataaagat gcagtaataa gaaggcgcta
     3421 ctggattgat gaaatagtaa aactaagcgg tcattttgtt aacgattcaa gtagggttga
     3481 aaatgaaata atcgatgagg ttaagaagtc cggaaatcaa gcgttgctag atcacctgag
     3541 attatgcaca gcaatacctg agtcatatgg tcatgattca agtgaagaga agctgtactc
     3601 aaagtataca gatgcactaa tatcagagtg ctttaaatac ttagggttaa atagcattgt
     3661 tttaaccgaa agggcagatg cagctgatgt tgaggttgtt tgcgataact attcatttgt
     3721 agccgatgca aaggtatttc gtttaagtag aacagccaaa aatcaaaagg attttaaagt
     3781 tcaagctatg gatggttgga gaaataccaa agactttgca atggtggtct gcccaatata
     3841 tcagctacca actaaatcaa g
//
