LOCUS       CP098951                4091 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB47-C06 plasmid unnamed4, complete
            sequence.
ACCESSION   CP098951
VERSION     CP098951.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488720
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB47-C06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:11:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,839
            CDSs (total)                      :: 4,721
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 57 of 142
            Pseudo Genes (incomplete)         :: 82 of 142
            Pseudo Genes (internal stop)      :: 38 of 142
            Pseudo Genes (multiple problems)  :: 31 of 142
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB47-C06"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(3689..4091,1..299))
                     /locus_tag="NFL56_24150"
     CDS             complement(join(3689..4091,1..299))
                     /locus_tag="NFL56_24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WGC08952.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(832..1122)
                     /locus_tag="NFL56_24155"
     CDS             complement(832..1122)
                     /locus_tag="NFL56_24155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WGC08953.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVREP
                     GETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="mobilization protein"
     gene            1293..3440
                     /locus_tag="NFL56_24160"
     CDS             1293..3440
                     /locus_tag="NFL56_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WGC08954.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDSN
                     AAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQL
                     RKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRSRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDKGLRFA
                     GYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEW
                     QRDEYARTHATERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     Typing          complement(1127..1277)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.81"
                     /coverage="85.0"
                     /evalue="3.53e-55"
     Typing          complement(1294..1648)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..299)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.629"
                     /coverage="99.0"
                     /evalue="6.93e-98"
     Typing          complement(3691..4033)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.471"
                     /coverage="99.0"
                     /evalue="3.35e-78"
     Typing          1252..3437
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.177"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tactggatcc agcggaactc ctcgcgcttc tcatcgtcct taaggatgat cgacctgtcc
       61 cacaaacgat caattgcttc ctgtagctga acctcggcat ttttccgccc catatcaggg
      121 aatgagtcga aatactcttc ggcggtaaca gtcatggtct tttgcaattc agtggattct
      181 gcatcagaac ccgacttcaa tcgaccaatg caaagtaaca agaatcgctg ttcctgaaga
      241 gtcaatttat agctggcctc aaccagtgca ttggatttgt aggcaatcag atccgacatt
      301 gtaaatccct caacctaagg taatgagtaa aatataaaca ttcctttagg taaaggcaat
      361 atgatttaat tttaatatcc cttttcctgt gaatattacc ttaaatcata ttgcctttac
      421 cttaaatcat attgcctatc caccttaaat catattgcct ttaccttaaa ttatattacc
      481 tatagtcttc taaactcttg aatttcataa gaaaattcga ccctaaaaca agtattaaaa
      541 acaagatctt aaaaacaaga tctaaaaact taacggcgag cctgtggaaa acttgggggg
      601 ttcaaaacaa aaaatcatgg aaaacaacag gttacaaaaa agcaggttcg caagctttag
      661 caaaaccccg ctcaaccctg tggaaatcca gtattcatgc gggttcagcg catatcacct
      721 gtcgaaaaac cgtgcgtatg tgctcaatct ggcgacggat tcacttccgc ttcctcgccc
      781 actcgctcgc taacgctcgg tcgctggggc tgcggcgagc ggacggtgca ttcacgcctg
      841 ttggtcgttt tctgacactt ctttggggac tggttttggt aacgcgttca gttcgtcaat
      901 caacggctgt atcctgctca catcagcctg ccgtgttacc tctgtcttca ggagatgaag
      961 caaccaggct cgtagttgtg tttccccagg ctccctgacc tttgccagca atgccgctcc
     1021 cacaatgacc ttctgcgctg tgtccaggct cctgctcttc gccttcagac gctgcaatct
     1081 ggcctcagca tcggcaatct tctgttcgag tgttctactc attccgtaac tccgtgcgcg
     1141 gtgaaaaatc gcattttagc gcgtcactgg tagtttaaaa actgaactgg cataatgcac
     1201 agcaaatcac gaagtgcgca cttatacaaa ctctccttcg tcgagtttgt gtgcgccctg
     1261 cggggctaaa agaaaacggc aacaacgaca cgatggcaat ttatcatctc agcatgaaga
     1321 ttctttccag aagcaagggc tacagcgccg ttgcttctgc tgcctatcgt gccggagaaa
     1381 aaatactgga tgagcgcacc ggagtgattc acgactacac ccgcaaaaac ggtgtagcca
     1441 gcgcagtgat tctgacgcca gcaaacgccc cagcatggtg tgcgaaccgc gccgaactct
     1501 ggaatgctgt tgaaaaagcc gagcggcgga aaaactcaca acttgcgcgg gagttcgaac
     1561 tcgccatccc ccgtgaactg gcgcaggatg ccgcacggga aacagttctc aacttcgtaa
     1621 gggaaaactt tgtcagtcgc ggcatgatcg ccgacgtggc ctttcacaat ctgggcgggt
     1681 caaacccaca cgcgcacatc atgctatcca tgagggcaat cacgcctgac ggcttcgggg
     1741 agaaagtccg tgagtggaat gactggactc acgccgaaac atggcgggga tcatgggcag
     1801 accatgcgaa cagggcgctg gcgaacgccg gttatcagga agagatagac catcgttcat
     1861 acgagcgaca gggggttgag aaaacgcctg gcattcacct cggaaagtcg gcctgtgcga
     1921 tggaaaagtg gggcatcgaa acagaacgcg gcgaacagaa tcgcctgatt aacaggctta
     1981 acctggaaat acaggtttcc cgcgacgaac tcaggaacag tgagctgaca gagcccgcac
     2041 gccgtatggc tgaccttctg aatattgagc ttcctgacaa cgcaacttcg tacatactgc
     2101 atgacatcat cgaagcgttg ccgacggaca gtaacgcagc atggaaactc accagtaaag
     2161 ccatgtcgat gatggcagat atgcaggcga cgcgcagacg ctgggaagat ctgaacaccg
     2221 aacgaaagga agcgatgagc catgctgcca gcctgaaaaa atcatcgccg ttttcgtccg
     2281 gtttttcccg tattcccctg atgcaatggg ttgcaccgga atacggccgc gaacaggaaa
     2341 aaatccagtc gttgagccag cgcatggaaa aattgcggca gcatcactgg aaggttgaga
     2401 aacaggacat tcccgcgagt caaaaagcat ttgaaacaca gtggcagcag tggggtgcat
     2461 ctggccttgc agatctccga gaacagttgc gtaaacgtga agaggaattg cgccggaagg
     2521 agcaggagga aacagaacgt cgtcgggcgg agcagctcag gagaaatgac aatgccgtta
     2581 tggattcagg ggtactgaaa gcagtcgtta cggggtatgg cgaggccccc atgccaggta
     2641 acggggaaat gacctgttac cttctgcttc acaaccggaa cggagaatat acgctctggg
     2701 ggaacgagct ggagaaatac cgctccagaa tttttgagag tgttgattta atgcgtgaca
     2761 ggagtggtta tatctgtgac cgaagcgaaa tcggtcaacg tccaccactg caacgtgttt
     2821 attcctctgc gacctttgag caactgctga cgcaggtttg ccagacatgg ccgcaacata
     2881 cccgtaatct ccgccagccg aaaacatggc cggagtcttt ctgtcttgga gaagaccgcc
     2941 agccagccat gccatcactg gcggcaagga aggtggattt tacgcaggga cgtctgccac
     3001 cgactctgat gccggtaatg agttctgtcg accgtgaaac ccgccagctt cagcttttgc
     3061 tggtcatggg tgtggatgac tctatgggcg gtgttgtccg gcttaatggt acgctgtatc
     3121 ccgcgtttgc cgtccccaca gccgacaaca gccagctggt aatcaatgca ctgaccgaca
     3181 aaggtttacg ctttgcaggc tatggtgaag cggtaaacca cgatgctgac agtccggcac
     3241 gtcctgcgcc ggaacttatg cagtttcatc tgaaaacctg gagcgaaccg ctttttgcta
     3301 tcgtcaacac gccagaaaaa cagccggatc atctgttccg ctcgctgggc tttgagcgca
     3361 cctgggatga atggcaacgt gatgaatatg cccgaacaca cgccaccgag aggagacatg
     3421 atcgcggttg gtcacagtga atgtgaccaa tattttgttt tgtttaacat cgtgttatgt
     3481 tcgtcgatgt tcatcgacag ggatgaatgc aagaagcaca cacataacac aaatcaactt
     3541 tatagttttc ccgcccccga aaaattgggg gttctttttt cactgtgcca gagcgtttat
     3601 gacgcaattt tttacttcac cagtaccttc gcatgccaaa cacaaaaaaa acgcgtcaga
     3661 gagcgtttta gaggccttaa aatcgattct agatcgtcat cttgatctgt ttgtcttcct
     3721 tgaagcgaaa atgcagggct acaaccgtgc gccccttctt gatcgtctca agcgttacag
     3781 ccaagtcgct tttttgattc agttcgtcaa cgcaaggtct taataattgc tggttcagag
     3841 acttatatgt ttgatattta tgctctatat caagcattga gcgaaaatca tcgagggcga
     3901 taatacgttc ccctgtgctg cggaattgct ggagaagttc gtatattctt atgctgtaac
     3961 ttcttgataa attggagatg ttcttaatta caactctagt aaattgcccc ttcaactgtg
     4021 tcagataagg catgaccgca tctgaaaaag tgatttgggc tttgccctca cctttagcgt
     4081 actgtgcccg a
//
