LOCUS       CP098950                4593 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB47-C06 plasmid unnamed3, complete
            sequence.
ACCESSION   CP098950
VERSION     CP098950.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488720
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB47-C06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:11:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,839
            CDSs (total)                      :: 4,721
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 57 of 142
            Pseudo Genes (incomplete)         :: 82 of 142
            Pseudo Genes (internal stop)      :: 38 of 142
            Pseudo Genes (multiple problems)  :: 31 of 142
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB47-C06"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4281..4593,1..206))
                     /locus_tag="NFL56_24120"
     CDS             complement(join(4281..4593,1..206))
                     /locus_tag="NFL56_24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WGC08949.1"
                     /translation="MKERAASTENMLNSEFRKLEESVDKALSLNRQKIRDAISEHTTSV
                     KQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTALSVTGGIFLGCWGL
                     IQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVLPDGVKGENTWTVGD
                     KNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(94..936)
                     /locus_tag="NFL56_24125"
                     /pseudo=""
     CDS             complement(94..936)
                     /locus_tag="NFL56_24125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(926..1249)
                     /locus_tag="NFL56_24130"
     CDS             complement(926..1249)
                     /locus_tag="NFL56_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WGC08951.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1312..1503
                     /locus_tag="NFL56_24135"
     CDS             1312..1503
                     /locus_tag="NFL56_24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WGC08950.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2514..3353
                     /locus_tag="NFL56_24140"
     CDS             2514..3353
                     /locus_tag="NFL56_24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGC08947.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(4041..4274)
                     /locus_tag="NFL56_24145"
     CDS             complement(4041..4274)
                     /locus_tag="NFL56_24145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WGC08948.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1592..1676)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          1687..2242
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.907"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2..936)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.423"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3944..4593)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.29e-125"
ORIGIN
        1 cgggacagtt cggcctctgt ggtgctgagc tgcgtgctca cggtggtgct cagcgtatcc
       61 agttgctgtt tcacggatgt tgtatgttcg ctgatagcgt ctctgatttt ctgcctgttt
      121 aagctcagtg ccttgtctac agactcttct agcttcctga actcgctgtt cagcatgttc
      181 tcggtagaag cggctcgctc tttcagtttc ttctcgaagt ccgctcccat ttgtaaaaga
      241 ttgctcatac agcgccccct ttagcctgag attccgcccc ccctccgggt cggcaatact
      301 gatactgctt ctggttgtcc tcaccacctc aaaacctgcc gctgtgagcg cctcagtgac
      361 atcctgacgc gtttttattg ctccggcacg gtaaagcgcc tcaagacccc gtgtaatcgc
      421 ttctgcggcc tcctgtttcg cttttggcag attattcggt gtgacaagcg tccgtctgtt
      481 ctccggtgcg ttcgggtcac gcagcccgta atgatgatta accagcgtct gccaggcatt
      541 tatcctcggc ctgtccgcac ggtcgtagta cggctggagg cgcttaccgc tctgtagttc
      601 catgttcgga atgacaaaat tcagttcgag ccgcccctta tcctggtgtt ccacccagag
      661 aatgctgtac tggtcttttt cgagaccggg cataagaaca cgctcaaagc tcgtcatcac
      721 tctttcgcgt tcacccggcg gtaactcctt ctccgcgaac gacagaacgc ctgacgtgta
      781 tttcttcgaa aatggcgtgg catcaatgag ttcccggatt tcttccgggt taccccggag
      841 caccgttgcg ccttcgcggt tacgctcccg ccccagcagg taatcgaccg ggccacttcc
      901 accgcctttc cccctggcat gaaatttaac tatcatcccg tcccccctgt tccctgacag
      961 cctgccgcaa ctggcgcaat tcaccctcga tggccatcag agcggccacc acatgaaccc
     1021 ggtcaatcga agaccagtgg ccgctgttca ccttccgggc tgtctggttc agattgttcc
     1081 cgatggcagc cagatgccgc agcaatggcg gcgatagtgt cggcagtttt cctgtgcgcg
     1141 caaccggctc ccccagacag acccgccgca tccattctgc cagtcgttta ccctcgcagc
     1201 gttccagtaa ccgggcgtgc tcctcatcgc taacccgtat cgtgagcatc ttctcgcgtt
     1261 tcatcggtat cattccccct aaaaaatccc ctattaagga gccttaagac catgaccaaa
     1321 caggaaaaaa cagcccttaa catggcccgc tttatcagaa gccagacgct gaccctgctg
     1381 gaaaaactca acgagctgga cgcggacgaa caggcagaca tctgcgaatc acttcacgac
     1441 cacgccgacg aactctaccg tagttgcctc gcacgcttcg gcgatgacgg tgaaagcaac
     1501 tgaaacatgc agctcccgga gacgttcaca gcttgcctgt gagcggatgc cgggagcaga
     1561 caagcccgtc agggcgcgtc agcgggtttt agcgggtgtc ggggcgcagc cctgacccag
     1621 tcacgtagcg atagcggagt gtatactggc ttaactatgt ggcatcagtg cggattataa
     1681 gaaaagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaatgccgca
     1741 tcaggcgctc ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cggctgcggc
     1801 gagcggtatc agctcactca aagacggtga tgcggttatc cacagaatca ggggataacg
     1861 ccaaaagaaa catgtgagca aaaggccagg aacaggaaga aggccgcgcc gctggcgttt
     1921 ttccataggc tccgcccccc tgacgagcat cacaaaaaca gatgctcaag tcagaggtgg
     1981 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2041 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2101 gtggcgcttt ctcatagctc acgctgttgg tatctcggcc cggtgtaggt cgttcgctcc
     2161 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2221 tatcgtcttg agtccaaccc ggaaagacac gtaaaaacgc cactggcagc agccattggt
     2281 aaccgtttag aggagttagt cttgaagtta tgcgccggtt aaggctaaac tgaaaggaca
     2341 gatttggtgg ctgtgctcct ccaagccagt taccttggtt caaagagttg ctgctctgag
     2401 aaccttcgaa aagccgcctt gctgggcggt tttttcgttt acggacaaga ctaccttcag
     2461 gatcgttaga tctcaagaca atcatgcata gtgagcatac acaggagccg aacttgctta
     2521 cactgttcat cttttttgtt ttattaatag ctgcatgttt cttttgtttt gctccaccac
     2581 gaagaggata tgacagaaat gagatcattc catacaaaat aaaactatca ataaataaat
     2641 atcgccttta catatactca tcgggaaagg taagacaata ccttttattt ttagttattt
     2701 tatcactgta ctactccata gcggaaccat ttaagtctga gttaataaaa aacatatcat
     2761 attcactgat ggctgcattt atttttgata cagggcttaa ttttagcaag gagaatataa
     2821 ctaaaggagt gatttcaaca aggtggcata atgacctata ttcatcattt gagagaatga
     2881 aagcaattaa taaaatttac tacccatcca acaaagagat aaacacagaa ggtctatcca
     2941 aagccatcac atcatctctt ttcaatgatg acgcgaactc gttcgcaaag agagattttc
     3001 gcttgatgtg ggacttatct tctgaaaaat atctttccta taaagaaata ataataagaa
     3061 aaggcgataa gcttgacgct gtatgcttga gatttataaa tgatgactac aagtttttag
     3121 ttaatttcaa cagagatgaa gaggttttca aatactttcc atctataatg caaccttcac
     3181 tcaaaacata tagagcgtta tcaagactag taaactcaat caaagatcct tctagattta
     3241 agttcacgac agagtcatta gaaatggagt tactagaata cttagagtta agaaatgaat
     3301 tattcaatga catagaagaa gtaatgggta gctatgctca gagggcacca tagttcatga
     3361 tagccctcga tagtacttac ttatattttt agagaggttt aagtgtaagt agagggagta
     3421 acacctggtt tttcagcatc cactccaatg aatgctgaca ccgatacgcg ataattctct
     3481 ttatcgtagc aaaatcatcc tccagattgc ggttgagaat aggttcatgg tgcgcaatac
     3541 gattgcgcag ttttctcacg ggacttgtta gcagtaatgc ttccccccgg ggacaaacga
     3601 cagagatata caacaaaggc ccgcaattaa aatactccgt atttaccata acgtcgcatt
     3661 gcgcgtaccg cttaaacgcg ttcagcgcga tcacggcagc agacaggtaa aaacagcaac
     3721 aaaccacccg aaaaactgcc gcgatcgcgc ctggtaaatt ttaacctcat gcatagttat
     3781 gcgcgaaact gaatgacgat cagtttcgca cgttccagcc gtcgaacggg gcaaaatggg
     3841 gaggtttgga tattgttaat gctaatgtac gtacattaac attggcgcca tccgggtagg
     3901 gcgtgccaca aactggctcg atttcagggg gtaccccggt cacatcccca ttgatgggcc
     3961 acgccagatc cgcgcttcgg gttcctgctc ttcatgccag cgccgggcaa gctcctgcgc
     4021 ctgacgctcc tgttcatccc ttatctgttg ttcgtgataa ataaccgact caagcggtcg
     4081 acttgtccgg ctaatagctg cacctgctga ctcaagttca cgacacgctc gttcagtatc
     4141 gcgttgttct gttgcgtaac cgcgtgcatc ttctgcaatt ctgcgaaggc gctctcccac
     4201 tcgttcagcc gccgcatata gtcctgttgc aaatgctcta aggcgttcag caaatgccct
     4261 tccagttccg tcatactcgt ttactccctg accagcctca cggcgttctt gtcgcccacc
     4321 gtccaggtgt tttccccttt caccccgtcc ggcagcacca gaaatttccc gttctcgcca
     4381 ttgacgaacg tcacccccca ggtttttgcc tcgagtttcg ccagcgtgtt ttcctgctct
     4441 ctgatggtca agatattttt cgcaatcctg ctttcctgcc actgaatcag cccccagcac
     4501 cccaggaaaa tgccgcccgt gacgctgagc gcggtcagcg ccggaaacag tacccgccct
     4561 ttgatcacct gccagagcag gtttttctgc tgc
//
