LOCUS       CP098945                3758 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB47-C09 plasmid unnamed5, complete
            sequence.
ACCESSION   CP098945
VERSION     CP098945.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488721
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB47-C09
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 132x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:23:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,868
            CDSs (total)                      :: 4,753
            Genes (coding)                    :: 4,518
            CDSs (with protein)               :: 4,518
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 69 of 235
            Pseudo Genes (incomplete)         :: 163 of 235
            Pseudo Genes (internal stop)      :: 50 of 235
            Pseudo Genes (multiple problems)  :: 44 of 235
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB47-C09"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3756..3758,1..243)
                     /locus_tag="NFL57_24300"
     CDS             join(3756..3758,1..243)
                     /locus_tag="NFL57_24300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGC13472.1"
                     /translation="MSADKPNKDSQDYIDSFDAMGRELPPAFPLSIRFGVVEDICIIDF
                     IDLPSKNTKKVFYSVAITKDHAKKMMSALKKFVDSE"
                     /name="hypothetical protein"
     gene            248..943
                     /locus_tag="NFL57_24305"
     CDS             248..943
                     /locus_tag="NFL57_24305"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGC13473.1"
                     /translation="MSICYNIEDVKIIDDDLEYSEPLFCSSLGIEGESACVEAYVELGK
                     GIDYSCNSYNNDVNVENYTILLYSKTESRLDLWNDILREINRKTSLRDVDYLYEILSVV
                     DKKGLAEYKSIISKICKVIDEYKGHLNDGVDLSSGAIFQLVRYIPFFFCKKNIDFYLEK
                     DTGFIGVIIPMRKNKKGTLNLTIMNTAEIYYSYVRKKTGLVKFSGKGFLGDDLKNSDAI
                     MSILRMSDW"
                     /name="hypothetical protein"
     gene            952..1416
                     /locus_tag="NFL57_24310"
     CDS             952..1416
                     /locus_tag="NFL57_24310"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGC13474.1"
                     /translation="MSSALRIIPDQVDCVDVELKTYLVWRYVKEDHRENDNAVSAVCFC
                     LTERDGGHVSFSHSNKDNVNEMVAEIHRNIHVAKKTKPALYLSIDIKEAYEEINNPDEL
                     ITFKGCGYPHIGMYYHDYSDVAKRNLIIASLVELSDLYFRAEGGGVSKVE"
                     /name="hypothetical protein"
     gene            complement(2329..2532)
                     /locus_tag="NFL57_24315"
     CDS             complement(2329..2532)
                     /locus_tag="NFL57_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WGC13471.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQATMCERLHELA
                     EELQNSIQTHFEAQYGIGVEQP"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2655..2733
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="83.0"
                     /evalue="3.6300000000000003e-22"
     Typing          complement(1707..2197)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.129"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 tcggcagata agccaaataa agatagtcag gattatattg attcttttga tgctatggga
       61 cgggaattgc cgccagcatt tcctttatca ataaggtttg gggttgttga agatatatgc
      121 attatagatt ttatagattt accatctaaa aatacgaaaa aagttttcta ctcggtggct
      181 ataacgaaag accatgcaaa gaaaatgatg tctgcactta aaaagtttgt ggattcggaa
      241 tagtaaaatg tctatatgct ataacattga ggatgtaaaa atcatcgatg atgatttaga
      301 atattccgag cctttgttct gtagttctct tggtattgag ggcgaatctg cttgtgttga
      361 agcttatgtg gaacttggca agggtattga ttattcctgt aattcatata acaatgatgt
      421 aaatgtagaa aattatacaa tacttcttta ctctaaaaca gaaagtcgcc ttgatctttg
      481 gaatgatata ttaagggaga taaatagaaa aacatcttta agagatgttg attatcttta
      541 tgaaatactt agtgtggtag ataagaaagg acttgctgaa tataaatcaa taatatctaa
      601 aatatgcaag gtaatagacg aatataaagg tcatcttaat gatggtgttg atttgtctag
      661 tggtgcgatt tttcaattgg ttagatatat tccattcttc ttttgtaaga agaatataga
      721 tttttatttg gagaaagaca ccggttttat tggggtgata ataccaatga gaaaaaataa
      781 aaagggaact ttgaatttga caataatgaa tactgcagag atttattact cctatgttag
      841 aaaaaaaaca ggtcttgtta aattctctgg aaaaggtttt ttgggggatg acttaaaaaa
      901 ttcagatgca attatgtcaa tattgagaat gtctgattgg tagggatagg gatgagttct
      961 gctttaagaa taataccaga tcaagttgat tgcgtagatg ttgaattaaa aacatacttg
     1021 gtatggaggt atgttaagga agatcataga gagaatgata atgcagtttc tgctgtttgc
     1081 ttctgcttaa cagagagaga cggtgggcat gtgtcatttt ctcattcaaa caaggataat
     1141 gttaatgaaa tggtagcgga gatccatcgt aatattcatg tggcaaaaaa aactaagcct
     1201 gctttatatt tatcaattga tattaaagaa gcgtatgagg aaataaataa cccagatgaa
     1261 ctaattacat ttaaggggtg tggttatcct catataggga tgtattatca tgattattct
     1321 gatgtggcta agcgcaactt gataatagcc tctcttgttg agttatcaga cctttacttt
     1381 cgagctgagg ggggtggggt tagtaaggtt gagtgagata aaattaacat ttgcggcact
     1441 ctttgaacag gatcttcttg agatcctttt ttggtgagcg taatctcttg ctctgaaaac
     1501 gaaaaaacca cccggcaagg tggtttttcg aaggttagtt aatcctggca gattttctaa
     1561 ccgtggtaac agtcttgtgc gagacatgcc accaaatctg tcctttcagt gtagccggag
     1621 taaggccatc acttcaagaa ctctcgatgc atctctcgca cattctgttt accaatggct
     1681 gctgccagtg gcgttaagtc gtgtctttcc gggttggact caagacgata gttaccggat
     1741 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1801 acctacaccg ggccgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1861 gggagaaagg cggacaggta tccggtaaac ggcagggtcg gaacaggaga gcgcacgagg
     1921 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     1981 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacggcatc
     2041 gtcgcagcct tttacggtcg tgctcttttg cccacatgtt ctgtcctggt tatcccccga
     2101 ttctgtggat aaactgcctg ccgttattag agagccgcca ccgctcgccg cagccccagc
     2161 gaccgaacgt agtgagcgag tgggcgagga agcggaattt ctccgcccgt ttgctttgag
     2221 cacttacgcg ccagtgtctt tctcaggtca ctgtcctgac gcgccctgcg ggcttgtctg
     2281 ctggcgaaaa cgctcacgga caagccgcga ccgctttcgc cagcgtcttt atggttgttc
     2341 cactccaatg ccgtactgcg cttcaaaatg cgtctgtatg ctgttctgca gttcttccgc
     2401 cagttcgtgc agccgttcac acatggttgc ctgttcgtcg gcatcgaggg catccagctt
     2461 ttcaagcagt gtcagactct ggcttttgat gaatcgcgcc atgttaagtg cggtttgctg
     2521 ctgtttgttc accttctgtc gttctccgtt ctgtctctgt attcagcatc atgtgattat
     2581 gccgtgttcc gttctttgcc tggacagtga gcgtttttcc ggttgtttga tgttcatccg
     2641 ttcatcacat cgtgggtgtc ggggtaaagc tctgaccagg ttgtaatcgg atgaacgtgc
     2701 gtgcgcggtt atacgattac acatcctgtc ccgttcatgg ggtcgtgtta ccggcagttg
     2761 ttgcgtcgct cctgtttcgt gccgtaaatc ttttgcggat gtgacggggt acagttgtac
     2821 cccctgccgg actgttgcca ccagggcgac cgtatccggc tgcctgacac aggacaggat
     2881 gtgtaattgt aataccgtca cccgtgacgc tattacaatt acctcctgct cagggtttca
     2941 ccccgatacc ccggcgtaat gctctgacgg ccagcgcaaa tttattcgct ttttctgaga
     3001 gtcctgtagc ccgaaccagg accggctccg cgcctgaaat tcccctctcc tctgacctgc
     3061 atttaccaca cctgtcgcaa aactgcgttt tgcgtgattt tactttaaat tcagtgcgtt
     3121 atagtggcgt ttatctggca aaaaattcta ttttgtcgga tgtggtgggc tgatgatggg
     3181 cttctacgcc cacagttagc ccatcatcag cccacttttg tgggttttat attgataatc
     3241 atagtgatag caaaagtgtg ggcgttttgt gggctaatag agtgggctga tggtggcctc
     3301 ttatttgtat gaaatataac ggataaattt ttatgataca tcggtgtgcc catcggtatt
     3361 ttgtgtgtgg gctaatggtg ggctgttgaa tgtcgttgct gacggatgtg ttctggcatg
     3421 gaatgtgacg cagacagaaa gaaaactgcc aaaaataagc ctcagggaca acggacgcgc
     3481 ccagaccgat tgccattcag tttcatgcat agtcatgcaa cgcgctgaaa acaatcctga
     3541 cgcatttttc cggtttaccc cggggaaagc atcccttttt acggtttttg cgccagaatc
     3601 gcgctgagtg cgttttggcg gtgcgcgtaa tgcgacgtta tggtaaatgg tatgatgttg
     3661 catgttacat tatggatgtt cgtggtgttt gagtagtagt tttttaggtt gaatgttaaa
     3721 ctttaaatgt gtctattttg ttatggacgg ttgatatg
//
