LOCUS       CP098944                4074 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB47-C09 plasmid unnamed4, complete
            sequence.
ACCESSION   CP098944
VERSION     CP098944.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488721
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB47-C09
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 132x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:23:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,868
            CDSs (total)                      :: 4,753
            Genes (coding)                    :: 4,518
            CDSs (with protein)               :: 4,518
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 69 of 235
            Pseudo Genes (incomplete)         :: 163 of 235
            Pseudo Genes (internal stop)      :: 50 of 235
            Pseudo Genes (multiple problems)  :: 44 of 235
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB47-C09"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2614..4074,1..699))
                     /locus_tag="NFL57_24285"
     CDS             complement(join(2614..4074,1..699))
                     /locus_tag="NFL57_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WGC13468.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            878..1132
                     /locus_tag="NFL57_24290"
     CDS             878..1132
                     /locus_tag="NFL57_24290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WGC13469.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     gene            1664..2356
                     /locus_tag="NFL57_24295"
     CDS             1664..2356
                     /locus_tag="NFL57_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WGC13470.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVEELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(342..700)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          701..877
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          1665..2356
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.671"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..786)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="1.39e-172"
ORIGIN
        1 tatttccagg ttaagcctgt taatcagacg attctgttcg ccgcgttctg tttcgatacc
       61 gcgtgtttcc atcgcacagg cagactttcc gaggtgaata ccaggcgttt tctcaagccc
      121 ctgtcgctcg tatgaacgat ggtctatctc ttcctgataa ccggcgttcg ccagtgctct
      181 gtttgcatgg tcagcccatg atgcacgcca cgtctcggca tgtgtccggt cgttccagtc
      241 cctgaccttt ccgccgaacc cagcaggtcc gacagccctc gtggtcagca tgatgtgcgc
      301 atggggattt gttttatcca tgtgatggaa cgccacatca gcaatcatgc cctgactgac
      361 aaagttttct ctgacaaagg cgagaacggt ttcccgtgcg gcatcctgcg gcagttcacg
      421 ggggatagcg agttcaattt cccgcgcaag ttgtgagttc ttccggcatt cggccttctc
      481 aacggcattc cagagttcgg cacggttcgt acaccaggca ggggctgtcg cgggggtcag
      541 aatcacagct tcagccacac cgcttttgcg ggtgtagtcg tgagtcagtc cggtacgctc
      601 atcaagcatc agtgaaccgg aacgataggc agcggaagct acggcgctgt agccgctgct
      661 tctggagata attttcatgc tgagatgata aatcgccatt tctgccgtaa tgcctttttg
      721 tagttttctt tcagccccgc agggcgcaca caatcgaaac ggagtggaga ttgtataagt
      781 gcgcactttg tgatttgtcg tgcattatgc cagctcagtt tttaaactac cagtgacgcg
      841 ctaaaatgcg atttttcatc gcgcacggag ttacatcatg agcagaacgc tcgaacagaa
      901 gattgctgat gctgaggcga gattacagcg cctgaaggcg aagagccgga gcctggacac
      961 agcgcagaag gttgttgtgg gtgcagcatt gctggcaaag gtcagaaagc cggaggaggt
     1021 gcagttgcgt gcctggttac tccagttcct gaaggcagag gtgacacgac aggctgatgt
     1081 gacgcgcata ctgccgctga ttaacgagct ggaggcgctg ccaggacagt gattgtatcg
     1141 tccgctcgcc gcagccccag cgaccgagcg ttagcgagcg agtgggcgag gaagcggaag
     1201 tgaatccgtc gccaggttga gcacatacgc acggtttttc ggtaggtgat atgcgctgaa
     1261 cccgcatgaa tactggattt ccacagggtt ggaaggggag tattcggaac ttgcaattct
     1321 gctttcttgc aacacattgt ttttattggt tttttgtttt cagcgcccca agttatccac
     1381 aggctcgccg ttaagttttt agatcttgtt tttaagatct tgtttttaat acttgtttta
     1441 gggtcaaaat tcattaataa aatcaatatg ttatagcggt atatgtggag gaatttaagg
     1501 taaaggtgga ggaatttaag gtgtttaggt ggaggaattt aaggtaaagg tggaggaatt
     1561 taaggtgtta ttcacagaaa tgtgaggata taaaaaaaaa tattccacat ttcttatttg
     1621 tggggcacat taaagcatca ccacatttga acgggatctt tgtatgtcgg acttgattgc
     1681 ctacaagtcg aatgctttgg ttgaggccag ctacaagttg accctacagg aacaacgttt
     1741 tctgttgctg tgtatcagtc gattgaaatc tggtgctgat gctgaattgc aaaagacaat
     1801 gaccattacc gctgcggaat attttgattc attccctgac atggggcgga aaaatgccga
     1861 ggttcagtta caggaagcga tcgatcgttt atgggacagg tcgatcattc tcaagaatga
     1921 cgagaagcgt gaggagttcc gctggatcca gtatcgggca cagtacgcta aaggtgaggc
     1981 caaggctcga ataacctttt ctgacgcggt aatgccttat ctgacgcaac tgaaggggca
     2041 attcacgcgc gttgttataa aaaacatatc aggtttatca agtagttaca gtataagaat
     2101 atacgagttc cttcagcaat tccgcagtac gggagagaga attattgctc ttaatgactt
     2161 tcgttcaatg ttagggattg aaaataaata caaacaattc agagacttaa ataaaatact
     2221 aataaaaccc tgtgttgaag aactgaacaa aaaaagcgac ctggctgtaa cggttgagac
     2281 gatcaaaaag gggcgcacgg ttgtagccct gcattttcgc ttcaaggaag acaaacagat
     2341 caagatgacg atctaaaatc gattttaagg cacctaaaac gctccctgct gcgttttttt
     2401 tgtgttagta gtgcgaaggt actattgaag tgaaaaattg cgtcaaaagc gctctaatgc
     2461 agcgaaaaaa gaacccccaa tttttggggg cggaaaaaca aaactatgct tgcattatgt
     2521 gtgtgtgctt cttgcattca tccctgtcga tgaacatcga agaacatcga cgaacataac
     2581 acgatgttaa acaaaacaaa atattgttca cattcactgt gaccaaccgc gatcatgtct
     2641 cctctcggtg gtgtgtgttc ttgcatcttc ctcgcgccgc cattcatccc aggtacgatt
     2701 gaaacccaga gagcggaaca gatgatccgg ctgtttttct ggtgtgttga cggcagcaaa
     2761 aagcggtgcc tcacgggttt tcagatgaaa ctccataagt tccggcgcag gtgatatgtg
     2821 gctgtctgca tcgtgattta cggcaacacc ataccctgca taccgtaacc ccttatcggt
     2881 cagtgcactg atgaccagct ggctgttatc ggctgaggga acggcaaacg cgggatacag
     2941 cgtaccatta agccggacaa caccgcccag agagtcatcc acgcccatga ccagcaaaag
     3001 ctgaagctgg cgggtttccc ggtcgacaga actcattacc ggcatcagcg tcggtagcag
     3061 acgtccctgc gtaaaatcca ccttccttgc tgccagtgat ggcatggctg gctggcggtc
     3121 ttctccaaga cagaaagact ccggccatgt cttcggctgg cggagattgc gggtatattg
     3181 cggccatgtc tggcaaacct gcgtcagaag ttgctcaaag gtcgcagagg aataaacacg
     3241 ttgcagtggt ggatgttgac cgatttcgct tcggtcacag atataaccac tcctgtcacg
     3301 cattaaatca acacgctcaa agattctggt gcggtatttc tccagctcgt tcccccagag
     3361 cgtgtattct ccgttccggt tgtgaagcag aaggtaacag gtcatttccc cgttaccagg
     3421 catgggggcc tcgccatacc ccgtaacgac tgcgctcagt acccctgaat ccataacggc
     3481 attgtcattt ttcctgagct gttccgctcg tcgctccgcg cgactacgtt ctgtatcctc
     3541 ctgctccttc cggcgcaatt cctcttcacg tttaagcaac tggactctga gagctgcaag
     3601 gccggatgcc ccccactgct gccactgttt ttcaaatgcc gtctgactcg cgggaatgtc
     3661 ctgtttctcc accttccggt aatgctgtcg caatttttcc atttgctggc tcagcgactg
     3721 gatttttccc tgttcgctac ggtattccgg tgccgcccat tgcatcaggg gaatacggga
     3781 aaaaccggac gaaaacggcg atgatttttt caggctggca gcatggctca tcgcttcctt
     3841 tcgttcggtg ttcagctctt cccagcgtct gcgcgtcgcc tgcatatctg ccatcatcga
     3901 catggcatta ctggtgagtt tccatgccgc attactgtcc ttcggcaacg cttcgatgat
     3961 gtcacgtagt gtgtacgaag ttgcgttgtc aggaagcacg atattcagaa ggtcagccac
     4021 acggcgggca ggctctgtca gcccgctgtt cctgagttca gtgcgggaaa tctg
//
