LOCUS       CP098938                3758 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB47-C15 plasmid unnamed5, complete
            sequence.
ACCESSION   CP098938
VERSION     CP098938.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488724
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB47-C15
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:29:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,735
            Genes (coding)                    :: 4,492
            CDSs (with protein)               :: 4,492
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 70 of 243
            Pseudo Genes (incomplete)         :: 166 of 243
            Pseudo Genes (internal stop)      :: 56 of 243
            Pseudo Genes (multiple problems)  :: 45 of 243
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB47-C15"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3731..3758,1..218)
                     /locus_tag="NFL60_24210"
     CDS             join(3731..3758,1..218)
                     /locus_tag="NFL60_24210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGC17964.1"
                     /translation="MSADKPNKDSQDYIDSFDAMGRELPPAFPLSIRFGVVEDICIIDF
                     IDLPSKNTKKVFYSVAITKDHAKKMMSALKKFVDSE"
                     /name="hypothetical protein"
     gene            223..918
                     /locus_tag="NFL60_24215"
     CDS             223..918
                     /locus_tag="NFL60_24215"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGC17965.1"
                     /translation="MSICYNIEDVKIIDDDLEYSEPLFCSSLGIEGESACVEAYVELGK
                     GIDYSCNSYNNDVNVENYTILLYSKTESRLDLWNDILREINRKTSLRDVDYLYEILSVV
                     DKKGLAEYKSIISKICKVIDEYKGHLNDGVDLSSGAIFQLVRYIPFFFCKKNIDFYLEK
                     DTGFIGVIIPMRKNKKGTLNLTIMNTAEIYYSYVRKKTGLVKFSGKGFLGDDLKNSDAI
                     MSILRMSDW"
                     /name="hypothetical protein"
     gene            927..1391
                     /locus_tag="NFL60_24220"
     CDS             927..1391
                     /locus_tag="NFL60_24220"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGC17966.1"
                     /translation="MSSALRIIPDQVDCVDVELKTYLVWRYVKEDHRENDNAVSAVCFC
                     LTERDGGHVSFSHSNKDNVNEMVAEIHRNIHVAKKTKPALYLSIDIKEAYEEINNPDEL
                     ITFKGCGYPHIGMYYHDYSDVAKRNLIIASLVELSDLYFRAEGGGVSKVE"
                     /name="hypothetical protein"
     gene            complement(2304..2507)
                     /locus_tag="NFL60_24225"
     CDS             complement(2304..2507)
                     /locus_tag="NFL60_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WGC17963.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQATMCERLHELA
                     EELQNSIQTHFEAQYGIGVEQP"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2630..2708
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="83.0"
                     /evalue="3.6300000000000003e-22"
     Typing          complement(1682..2172)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.129"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 gtcaggatta tattgattct tttgatgcta tgggacggga attgccgcca gcatttcctt
       61 tatcaataag gtttggggtt gttgaagata tatgcattat agattttata gatttaccat
      121 ctaaaaatac gaaaaaagtt ttctactcgg tggctataac gaaagaccat gcaaagaaaa
      181 tgatgtctgc acttaaaaag tttgtggatt cggaatagta aaatgtctat atgctataac
      241 attgaggatg taaaaatcat cgatgatgat ttagaatatt ccgagccttt gttctgtagt
      301 tctcttggta ttgagggcga atctgcttgt gttgaagctt atgtggaact tggcaagggt
      361 attgattatt cctgtaattc atataacaat gatgtaaatg tagaaaatta tacaatactt
      421 ctttactcta aaacagaaag tcgccttgat ctttggaatg atatattaag ggagataaat
      481 agaaaaacat ctttaagaga tgttgattat ctttatgaaa tacttagtgt ggtagataag
      541 aaaggacttg ctgaatataa atcaataata tctaaaatat gcaaggtaat agacgaatat
      601 aaaggtcatc ttaatgatgg tgttgatttg tctagtggtg cgatttttca attggttaga
      661 tatattccat tcttcttttg taagaagaat atagattttt atttggagaa agacaccggt
      721 tttattgggg tgataatacc aatgagaaaa aataaaaagg gaactttgaa tttgacaata
      781 atgaatactg cagagattta ttactcctat gttagaaaaa aaacaggtct tgttaaattc
      841 tctggaaaag gttttttggg ggatgactta aaaaattcag atgcaattat gtcaatattg
      901 agaatgtctg attggtaggg atagggatga gttctgcttt aagaataata ccagatcaag
      961 ttgattgcgt agatgttgaa ttaaaaacat acttggtatg gaggtatgtt aaggaagatc
     1021 atagagagaa tgataatgca gtttctgctg tttgcttctg cttaacagag agagacggtg
     1081 ggcatgtgtc attttctcat tcaaacaagg ataatgttaa tgaaatggta gcggagatcc
     1141 atcgtaatat tcatgtggca aaaaaaacta agcctgcttt atatttatca attgatatta
     1201 aagaagcgta tgaggaaata aataacccag atgaactaat tacatttaag gggtgtggtt
     1261 atcctcatat agggatgtat tatcatgatt attctgatgt ggctaagcgc aacttgataa
     1321 tagcctctct tgttgagtta tcagaccttt actttcgagc tgaggggggt ggggttagta
     1381 aggttgagtg agataaaatt aacatttgcg gcactctttg aacaggatct tcttgagatc
     1441 cttttttggt gagcgtaatc tcttgctctg aaaacgaaaa aaccacccgg caaggtggtt
     1501 tttcgaaggt tagttaatcc tggcagattt tctaaccgtg gtaacagtct tgtgcgagac
     1561 atgccaccaa atctgtcctt tcagtgtagc cggagtaagg ccatcacttc aagaactctc
     1621 gatgcatctc tcgcacattc tgtttaccaa tggctgctgc cagtggcgtt aagtcgtgtc
     1681 tttccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1741 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgggccg agatacctac
     1801 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1861 taaacggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1921 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1981 cgtcaggggg gcggagccta tggaaaaacg gcatcgtcgc agccttttac ggtcgtgctc
     2041 ttttgcccac atgttctgtc ctggttatcc cccgattctg tggataaact gcctgccgtt
     2101 attagagagc cgccaccgct cgccgcagcc ccagcgaccg aacgtagtga gcgagtgggc
     2161 gaggaagcgg aatttctccg cccgtttgct ttgagcactt acgcgccagt gtctttctca
     2221 ggtcactgtc ctgacgcgcc ctgcgggctt gtctgctggc gaaaacgctc acggacaagc
     2281 cgcgaccgct ttcgccagcg tctttatggt tgttccactc caatgccgta ctgcgcttca
     2341 aaatgcgtct gtatgctgtt ctgcagttct tccgccagtt cgtgcagccg ttcacacatg
     2401 gttgcctgtt cgtcggcatc gagggcatcc agcttttcaa gcagtgtcag actctggctt
     2461 ttgatgaatc gcgccatgtt aagtgcggtt tgctgctgtt tgttcacctt ctgtcgttct
     2521 ccgttctgtc tctgtattca gcatcatgtg attatgccgt gttccgttct ttgcctggac
     2581 agtgagcgtt tttccggttg tttgatgttc atccgttcat cacatcgtgg gtgtcggggt
     2641 aaagctctga ccaggttgta atcggatgaa cgtgcgtgcg cggttatacg attacacatc
     2701 ctgtcccgtt catggggtcg tgttaccggc agttgttgcg tcgctcctgt ttcgtgccgt
     2761 aaatcttttg cggatgtgac ggggtacagt tgtaccccct gccggactgt tgccaccagg
     2821 gcgaccgtat ccggctgcct gacacaggac aggatgtgta attgtaatac cgtcacccgt
     2881 gacgctatta caattacctc ctgctcaggg tttcaccccg ataccccggc gtaatgctct
     2941 gacggccagc gcaaatttat tcgctttttc tgagagtcct gtagcccgaa ccaggaccgg
     3001 ctccgcgcct gaaattcccc tctcctctga cctgcattta ccacacctgt cgcaaaactg
     3061 cgttttgcgt gattttactt taaattcagt gcgttatagt ggcgtttatc tggcaaaaaa
     3121 ttctattttg tcggatgtgg tgggctgatg atgggcttct acgcccacag ttagcccatc
     3181 atcagcccac ttttgtgggt tttatattga taatcatagt gatagcaaaa gtgtgggcgt
     3241 tttgtgggct aatagagtgg gctgatggtg gcctcttatt tgtatgaaat ataacggata
     3301 aatttttatg atacatcggt gtgcccatcg gtattttgtg tgtgggctaa tggtgggctg
     3361 ttgaatgtcg ttgctgacgg atgtgttctg gcatggaatg tgacgcagac agaaagaaaa
     3421 ctgccaaaaa taagcctcag ggacaacgga cgcgcccaga ccgattgcca ttcagtttca
     3481 tgcatagtca tgcaacgcgc tgaaaacaat cctgacgcat ttttccggtt taccccgggg
     3541 aaagcatccc tttttacggt ttttgcgcca gaatcgcgct gagtgcgttt tggcggtgcg
     3601 cgtaatgcga cgttatggta aatggtatga tgttgcatgt tacattatgg atgttcgtgg
     3661 tgtttgagta gtagtttttt aggttgaatg ttaaacttta aatgtgtcta ttttgttatg
     3721 gacggttgat atgtcggcag ataagccaaa taaagata
//
