LOCUS       CP098937                4074 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB47-C15 plasmid unnamed4, complete
            sequence.
ACCESSION   CP098937
VERSION     CP098937.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488724
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB47-C15
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:29:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,735
            Genes (coding)                    :: 4,492
            CDSs (with protein)               :: 4,492
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 70 of 243
            Pseudo Genes (incomplete)         :: 166 of 243
            Pseudo Genes (internal stop)      :: 56 of 243
            Pseudo Genes (multiple problems)  :: 45 of 243
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB47-C15"
                     /isolation_source="Pooled faeces sample collected from
                     floor of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(3211..4074,1..1296))
                     /locus_tag="NFL60_24195"
     CDS             complement(join(3211..4074,1..1296))
                     /locus_tag="NFL60_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WGC17961.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            1475..1729
                     /locus_tag="NFL60_24200"
     CDS             1475..1729
                     /locus_tag="NFL60_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WGC17962.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     gene            2261..2953
                     /locus_tag="NFL60_24205"
     CDS             2261..2953
                     /locus_tag="NFL60_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WGC17960.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVEELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(939..1297)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          1298..1474
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          2262..2953
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.671"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..1296)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.769"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(3215..4074)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.652"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggcattgtca tttttcctga gctgttccgc tcgtcgctcc gcgcgactac gttctgtatc
       61 ctcctgctcc ttccggcgca attcctcttc acgtttaagc aactggactc tgagagctgc
      121 aaggccggat gccccccact gctgccactg tttttcaaat gccgtctgac tcgcgggaat
      181 gtcctgtttc tccaccttcc ggtaatgctg tcgcaatttt tccatttgct ggctcagcga
      241 ctggattttt ccctgttcgc tacggtattc cggtgccgcc cattgcatca ggggaatacg
      301 ggaaaaaccg gacgaaaacg gcgatgattt tttcaggctg gcagcatggc tcatcgcttc
      361 ctttcgttcg gtgttcagct cttcccagcg tctgcgcgtc gcctgcatat ctgccatcat
      421 cgacatggca ttactggtga gtttccatgc cgcattactg tccttcggca acgcttcgat
      481 gatgtcacgt agtgtgtacg aagttgcgtt gtcaggaagc acgatattca gaaggtcagc
      541 cacacggcgg gcaggctctg tcagcccgct gttcctgagt tcagtgcggg aaatctgtat
      601 ttccaggtta agcctgttaa tcagacgatt ctgttcgccg cgttctgttt cgataccgcg
      661 tgtttccatc gcacaggcag actttccgag gtgaatacca ggcgttttct caagcccctg
      721 tcgctcgtat gaacgatggt ctatctcttc ctgataaccg gcgttcgcca gtgctctgtt
      781 tgcatggtca gcccatgatg cacgccacgt ctcggcatgt gtccggtcgt tccagtccct
      841 gacctttccg ccgaacccag caggtccgac agccctcgtg gtcagcatga tgtgcgcatg
      901 gggatttgtt ttatccatgt gatggaacgc cacatcagca atcatgccct gactgacaaa
      961 gttttctctg acaaaggcga gaacggtttc ccgtgcggca tcctgcggca gttcacgggg
     1021 gatagcgagt tcaatttccc gcgcaagttg tgagttcttc cggcattcgg ccttctcaac
     1081 ggcattccag agttcggcac ggttcgtaca ccaggcaggg gctgtcgcgg gggtcagaat
     1141 cacagcttca gccacaccgc ttttgcgggt gtagtcgtga gtcagtccgg tacgctcatc
     1201 aagcatcagt gaaccggaac gataggcagc ggaagctacg gcgctgtagc cgctgcttct
     1261 ggagataatt ttcatgctga gatgataaat cgccatttct gccgtaatgc ctttttgtag
     1321 ttttctttca gccccgcagg gcgcacacaa tcgaaacgga gtggagattg tataagtgcg
     1381 cactttgtga tttgtcgtgc attatgccag ctcagttttt aaactaccag tgacgcgcta
     1441 aaatgcgatt tttcatcgcg cacggagtta catcatgagc agaacgctcg aacagaagat
     1501 tgctgatgct gaggcgagat tacagcgcct gaaggcgaag agccggagcc tggacacagc
     1561 gcagaaggtt gttgtgggtg cagcattgct ggcaaaggtc agaaagccgg aggaggtgca
     1621 gttgcgtgcc tggttactcc agttcctgaa ggcagaggtg acacgacagg ctgatgtgac
     1681 gcgcatactg ccgctgatta acgagctgga ggcgctgcca ggacagtgat tgtatcgtcc
     1741 gctcgccgca gccccagcga ccgagcgtta gcgagcgagt gggcgaggaa gcggaagtga
     1801 atccgtcgcc aggttgagca catacgcacg gtttttcggt aggtgatatg cgctgaaccc
     1861 gcatgaatac tggatttcca cagggttgga aggggagtat tcggaacttg caattctgct
     1921 ttcttgcaac acattgtttt tattggtttt ttgttttcag cgccccaagt tatccacagg
     1981 ctcgccgtta agtttttaga tcttgttttt aagatcttgt ttttaatact tgttttaggg
     2041 tcaaaattca ttaataaaat caatatgtta tagcggtata tgtggaggaa tttaaggtaa
     2101 aggtggagga atttaaggtg tttaggtgga ggaatttaag gtaaaggtgg aggaatttaa
     2161 ggtgttattc acagaaatgt gaggatataa aaaaaaatat tccacatttc ttatttgtgg
     2221 ggcacattaa agcatcacca catttgaacg ggatctttgt atgtcggact tgattgccta
     2281 caagtcgaat gctttggttg aggccagcta caagttgacc ctacaggaac aacgttttct
     2341 gttgctgtgt atcagtcgat tgaaatctgg tgctgatgct gaattgcaaa agacaatgac
     2401 cattaccgct gcggaatatt ttgattcatt ccctgacatg gggcggaaaa atgccgaggt
     2461 tcagttacag gaagcgatcg atcgtttatg ggacaggtcg atcattctca agaatgacga
     2521 gaagcgtgag gagttccgct ggatccagta tcgggcacag tacgctaaag gtgaggccaa
     2581 ggctcgaata accttttctg acgcggtaat gccttatctg acgcaactga aggggcaatt
     2641 cacgcgcgtt gttataaaaa acatatcagg tttatcaagt agttacagta taagaatata
     2701 cgagttcctt cagcaattcc gcagtacggg agagagaatt attgctctta atgactttcg
     2761 ttcaatgtta gggattgaaa ataaatacaa acaattcaga gacttaaata aaatactaat
     2821 aaaaccctgt gttgaagaac tgaacaaaaa aagcgacctg gctgtaacgg ttgagacgat
     2881 caaaaagggg cgcacggttg tagccctgca ttttcgcttc aaggaagaca aacagatcaa
     2941 gatgacgatc taaaatcgat tttaaggcac ctaaaacgct ccctgctgcg ttttttttgt
     3001 gttagtagtg cgaaggtact attgaagtga aaaattgcgt caaaagcgct ctaatgcagc
     3061 gaaaaaagaa cccccaattt ttgggggcgg aaaaacaaaa ctatgcttgc attatgtgtg
     3121 tgtgcttctt gcattcatcc ctgtcgatga acatcgaaga acatcgacga acataacacg
     3181 atgttaaaca aaacaaaata ttgttcacat tcactgtgac caaccgcgat catgtctcct
     3241 ctcggtggtg tgtgttcttg catcttcctc gcgccgccat tcatcccagg tacgattgaa
     3301 acccagagag cggaacagat gatccggctg tttttctggt gtgttgacgg cagcaaaaag
     3361 cggtgcctca cgggttttca gatgaaactc cataagttcc ggcgcaggtg atatgtggct
     3421 gtctgcatcg tgatttacgg caacaccata ccctgcatac cgtaacccct tatcggtcag
     3481 tgcactgatg accagctggc tgttatcggc tgagggaacg gcaaacgcgg gatacagcgt
     3541 accattaagc cggacaacac cgcccagaga gtcatccacg cccatgacca gcaaaagctg
     3601 aagctggcgg gtttcccggt cgacagaact cattaccggc atcagcgtcg gtagcagacg
     3661 tccctgcgta aaatccacct tccttgctgc cagtgatggc atggctggct ggcggtcttc
     3721 tccaagacag aaagactccg gccatgtctt cggctggcgg agattgcggg tatattgcgg
     3781 ccatgtctgg caaacctgcg tcagaagttg ctcaaaggtc gcagaggaat aaacacgttg
     3841 cagtggtgga tgttgaccga tttcgcttcg gtcacagata taaccactcc tgtcacgcat
     3901 taaatcaaca cgctcaaaga ttctggtgcg gtatttctcc agctcgttcc cccagagcgt
     3961 gtattctccg ttccggttgt gaagcagaag gtaacaggtc atttccccgt taccaggcat
     4021 gggggcctcg ccataccccg taacgactgc gctcagtacc cctgaatcca taac
//
