LOCUS       CP098930                3175 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB47-SO-C05 plasmid unnamed1, complete
            sequence.
ACCESSION   CP098930
VERSION     CP098930.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488728
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB47-SO-C05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 129x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 17:32:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,733
            CDSs (total)                      :: 4,612
            Genes (coding)                    :: 4,454
            CDSs (with protein)               :: 4,454
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 54 of 158
            Pseudo Genes (incomplete)         :: 100 of 158
            Pseudo Genes (internal stop)      :: 42 of 158
            Pseudo Genes (multiple problems)  :: 31 of 158
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB47-SO-C05"
                     /isolation_source="Pooled soil sample collected from floor
                     of poultry farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed1"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(577..1686)
                     /gene="wzz(fepE)"
                     /locus_tag="NFL64_23660"
     CDS             complement(577..1686)
                     /gene="wzz(fepE)"
                     /locus_tag="NFL64_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0016020 - membrane [Evidence IEA]; GO_process:
                     GO:0009103 - lipopolysaccharide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="WGC36453.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEGIDLFGLISVLWAAKIKIVGAIL
                     FFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDNAFDLF
                     IKKFQSVNSLNEYMRSSPYVMEMIKKNNISALELHRAIVELSKNMKSVDDNTSKKNDKS
                     SLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEILALD
                     EIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIATKLNI
                     KKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPSNAIIVI
                     LSSVIGGMLACGYILLRHAMEARKMSVIE"
                     /name="wzz(fepE)"
     gene            2533..2727
                     /locus_tag="NFL64_23665"
     CDS             2533..2727
                     /locus_tag="NFL64_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WGC36452.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAENETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2019..2267)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.4"
                     /coverage="86.0"
                     /evalue="9.46e-113"
     Typing          complement(2399..2497)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          complement(2808..2894)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.455"
                     /coverage="100.0"
                     /evalue="1.14e-31"
     Typing          20..525
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.333"
                     /coverage="99.0"
                     /evalue="8.02e-146"
     Typing          2915..3175
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.925"
                     /coverage="99.0"
                     /evalue="2.56e-81"
ORIGIN
        1 atcgacgctc aagtcagagg tggcgaaacc cgacaggact taaagatacc aggcgtttcc
       61 ccctggtggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc
      121 gctgttatgg ccgcgttcgt ctcattccac gcctgacact cagttccggg taggcagttc
      181 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      241 gtaactatcg tcttgagtcc aacccggtaa gacacgactt aacgccattg gcagcggcca
      301 ctggtaaaca ggatgtgcga gagatgtatt gagagttctt gaagtggcag ccttactgag
      361 gctacactag aaggacagat ttggtgacat gtctcgcaca agactgttac cacggttcag
      421 aagttcccca acttactgaa ccttcgaaaa accacctccc caggtggttt tttcgttttc
      481 tagagtagaa gactacggct gtaagggaat gatctcaaga agatcctttg aactttttat
      541 aaaagttgct tataaaatga tgcatcagca caacttttac tcaatcacac tcatctttct
      601 ggcctccatg gcatgacgta aaagtatata gccacatgca agcatccctc caatgacaga
      661 agaaagaatt acaattatgg cattacttgg cccatccttt ctgaccggta acgttgggct
      721 caattgatac tgaaacggca taaaatcaac atcccccact ttcgccacaa ccaattgatt
      781 cacaacatat tgacgatttc gcaactcccc actcaattcc gaaacatcct tgattgattt
      841 tttgatgttc aatttagttg ctataccatc agaaccgaga gccacaggaa aatctgggtc
      901 atccttcata atctgaccat tgctgtatac aggttttttt attccagccg cattagcaac
      961 ctccagtgaa taattgaggc gtcgaatatc tgcattcagc tggtttctaa tttttatctc
     1021 atccagtgca agaatttctt tttcaacatt agttttaact tctagtttat ttcttatatc
     1081 ttccatcaaa tcccttacaa caagggaaga aacataatta atatacccag acaacacatc
     1141 gtgagcttct ttacttgttg gagcagtaaa actcagagtc catgatacat acaatgatga
     1201 cttgtcgttt ttcttactgg tattatcatc aaccgacttc atatttttac tcaactcaac
     1261 aattgcacga tgcagttcga gagcgctaat gttattcttt ttaatcatct ccataacata
     1321 aggagatgat ctcatatact cattcaacga attaacggac tgaaatttct taataaacag
     1381 atcaaatgcg ttatcacgat tgatatcaaa atccactccc agaacatgaa gcttagaaag
     1441 ctccttattt aattcactcc attgaacaga ctcagcagga gtaaccacag cagaactagt
     1501 ccatttttgt ggcaaaacaa aagaaacaag aaagccaata caagcaaaaa atagaatcgc
     1561 cccaacaatt tttatttttg ctgcccataa aacgctaatc aagccaaaaa gatcaattcc
     1621 ttcattacca ttcggcaaca tgttgtaatt agaaaaatgt tcgtcagata cctttgagat
     1681 tttcattgaa aaaaaacaca atatattaac atatggtttt tttattctat catatgttag
     1741 gaaaaaacct aaaaaccatt cagcagacat ttaacataac attttattac gcgcaccgct
     1801 aaaacgcgct gaacgcgatt cagccgcaaa caccgcaaaa agatatgttt tccccggggt
     1861 aaaccggaaa aatgcgtcag gatcgctttc agctcactgc atagctatgc atgaaagtga
     1921 atgacgatcg gtttgggcgc atacagtgcc catgagacac attttcggac attttcagtt
     1981 cgtgaatccc tgactcattg ctacatatct gaacagacac ccctagcagc gcccccaggg
     2041 gcgtcctgta aaaaaacaca ccgcgccgct agcagcactc ctaatataag atgatgtttt
     2101 ttatgaaaat agtcagtacc acccctaaaa agcggtgttg gcgcagctac aacaccgcgc
     2161 cgacaccgct tttttaaata tcacaaagag agcaagaaga actaatttgt cataactctc
     2221 tatttataaa gaaaaatcag caaaaaattg tttttgcgtg gggtgtgcgc atttcaggca
     2281 tgagaacaga ctcgcgtttt tccggagtat cagcggaatt attgattaag aaacactcga
     2341 cgcagagcga aattttatgc aacagcggct gcactgcaca gggattattt atcagttgat
     2401 atgctgtgcc atgatacgtc ctaaagaaac aggacaggat gtgcaattgt aataccgtcg
     2461 cccgcaacgt cttacaatta ccacctgctc agggtccccc cgacacccgg aactgacgga
     2521 gaacaacgga aggtgaacaa acaacaacaa actgcgctga atatggcgcg atttatcaaa
     2581 agccagagcc tgacactgct tgaaaaactg gatgctctgg atgccgacga acaggcggcc
     2641 atgtgcgaac gactgcacga actcgcagaa gaactccaga acagcatcca gattcgcttt
     2701 gaggccgaaa atgaaacagg aacataacga agctgacgga ggcggtcacg gctttcccgt
     2761 gggcgttttc gtcagcagac aagcccgtca gggcgcgtca gggggtttta gcgggtgtcg
     2821 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactagct taattatgcg
     2881 gcgccagagc agatggtgag agaacagcga catatgaggt gtgaaatgct gcacagatac
     2941 gtaaggagaa aatgcagctt ccggcgcttt tccgcttcct cgctcactga ctcgctgcgc
     3001 tcggtcgttc ggctgcggcg agcggtgtct gctcacgcaa agacggatat acggttatcc
     3061 acagaatcag gggagaacac cggaaaaaac atgtgagcaa aaaagaggcc ccgaaaaaag
     3121 gccgcgccgg tggcgttttt ccataggctc cgcccccctg acaagcatca caaaa
//
