LOCUS       CP098531                4213 bp    DNA     circular BCT 12-JUL-2023
DEFINITION  Halomonas sp. KG2 plasmid pHaKG2_3, complete sequence.
ACCESSION   CP098531
VERSION     CP098531.1
DBLINK      BioProject: PRJNA846065
            BioSample: SAMN28867506
KEYWORDS    .
SOURCE      Halomonas sp. KG2
  ORGANISM  Halomonas sp. KG2
            Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
            Halomonadaceae; Halomonas.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Schmitz-Esser,S. and Schultz,D.L.
  TITLE     Complete genomes of cheese rind Halomonas and Pseudoalteromonas
            strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Schmitz-Esser,S. and Schultz,D.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2022) Department of Animal Science, Iowa State
            University, 3222 NSRIC, 1029 North University Boulevard, Ames, IA
            50011, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-AUG-2021
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 157x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/07/2022 17:07:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,082
            Genes (coding)                    :: 4,028
            CDSs (with protein)               :: 4,028
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 23 of 54
            Pseudo Genes (incomplete)         :: 34 of 54
            Pseudo Genes (internal stop)      :: 13 of 54
            Pseudo Genes (multiple problems)  :: 12 of 54
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Halomonas sp. KG2"
                     /mol_type="genomic DNA"
                     /strain="KG2"
                     /isolation_source="Cheese rind"
                     /db_xref="taxon:2951138"
                     /plasmid="pHaKG2_3"
                     /geo_loc_name="USA: Vermont"
                     /collection_date="2021-04"
     gene            100..789
                     /locus_tag="NDQ72_20805"
     CDS             100..789
                     /locus_tag="NDQ72_20805"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKD30516.1"
                     /translation="MLRLLFIALLGAAPVAAAIWYSWPQLDELHSIDYPLERVLLVGAA
                     LSWLLVVVLGSMLPLLRWIEHAERERIQRHAQRDMAKELGMAQFRAEAALATADEAERL
                     RNLAEQEARQAEQRAREKALTELEHERTVMQSRQEALNKQIAQIEQQQAQLDAVEAQYR
                     VWVEQQQAAVAQAEHERDQAREMMRNRLCGHERRLRQATGHEHMDFETLKMISPPRPVR
                     VRRHAAS"
                     /name="hypothetical protein"
     gene            complement(1006..1830)
                     /locus_tag="NDQ72_20810"
     CDS             complement(1006..1830)
                     /locus_tag="NDQ72_20810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKD30517.1"
                     /translation="MRGVPPPENATTAVFQLIAGPGANLPLFRLTTGLRPYRPAAFAPT
                     QQLSHTWLGQCQPSIRPGASHVSLLRQNTPPLTPDRRPGLHLTPAVAGFSAARDSKAAR
                     LLAPSFNMMARPARSWLCASYPDATSTAAITGLSYTAISTGCPIEYQRFQVSPPRPDAL
                     RTPSAAHQQGLRSTVSSSPQPGFPPVRNRQGLSCRHEARFRVAQPRPFGRPVDWVEPGD
                     FTPERPTFAVPARLGAHGFGSGSPTVGDCRASPVLAALGFSSRIRSQRNRNT"
                     /name="hypothetical protein"
     gene            complement(1990..2247)
                     /locus_tag="NDQ72_20815"
     CDS             complement(1990..2247)
                     /locus_tag="NDQ72_20815"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKD30518.1"
                     /translation="MIEQWHHDHADKLLTAVGWGSDKSPEWHAGLHNAVAWHLAEQPLG
                     HGRSTCPHALKTAQRDAWMAGWSFGQARCLEEGRDALLRA"
                     /name="hypothetical protein"
     gene            complement(2240..2839)
                     /locus_tag="NDQ72_20820"
     CDS             complement(2240..2839)
                     /locus_tag="NDQ72_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WKD30514.1"
                     /translation="MSKVVRYDKNPFIENMIVPIRGQAVRLSQLGKDDNILVNQATGEV
                     QGTHVTTYRRVDAEQFVKLFTANIALTFDLKAAGIKAFNVLLWCLQNRAVERDLIPLDK
                     IVLDEFLAEHSDRKPPIKLSQPTFWRGLAELEKAQIIAKHLRQGWYYINPNFAFNGNRI
                     AFTTVIERQQPKSKTEGDAHLQQPLPISTNEGESND"
                     /name="replication/maintenance protein RepL"
     gene            complement(3370..4047)
                     /locus_tag="NDQ72_20825"
     CDS             complement(3370..4047)
                     /locus_tag="NDQ72_20825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKD30515.1"
                     /translation="MRLLLRHLSRGYIYWTAGSIEAKKWPALEAKFLDRYLIHATAQQR
                     WRRKAKGQCNSYLVGLIDKTSPGKIWWWLLVTEGEGMAHDLENLQHSGSKRGRLTMPDA
                     DYEALKAPRPGNSPAWTWRITADSYAGWEARLASAVQNAPSDGGDALRQALHSLQRVPG
                     FAQSRKQAYALENSAVKRWKRLNRGEWPHKRLHIGWLGRYTQGETVKAESIAARTKTTK
                     TSV"
                     /name="hypothetical protein"
ORIGIN
        1 gattcctagc gctgcgcgct atgcggcctt gccaggccgc ccaaaattcg cagaattttt
       61 gccgccgata ggcggctggt ttttgcctgg gaggcgccaa tgctgagatt gttgttcatc
      121 gcgctgctgg gcgctgcacc agtcgcggca gcgatctggt acagctggcc gcagctcgat
      181 gaactccaca gcattgacta cccgctggag cgggtgctgc tcgtcggtgc ggccctgagc
      241 tggctgctgg tcgttgtgct gggcagcatg ctgccgctat tgcgctggat tgagcacgct
      301 gagcgtgagc gaattcagcg tcacgctcag cgggatatgg ccaaagagct gggcatggca
      361 cagttccgcg ccgaggcagc gttggccact gcagatgagg ccgagcgcct gcgcaactta
      421 gctgagcaag aggcacggca agctgagcag cgagcgaggg agaaggcact gaccgagtta
      481 gagcatgaac ggacggtcat gcagtcacgg caggaagcgc tcaacaaaca gatcgcccag
      541 attgagcagc agcaggccca gctcgatgcg gttgaggctc aatatcgagt ctgggttgag
      601 cagcagcaag ccgctgtcgc ccaggctgag catgagcgcg atcaggcccg tgagatgatg
      661 cgaaaccgcc tgtgtggcca tgagcgccgc ttgcggcagg cgacaggcca cgagcacatg
      721 gattttgaga cgttgaagat gatcagccct ccgcgccccg tgcgcgtacg gcggcatgca
      781 gcgtcttagc tgagcaagtc ccctgacaca cgggatccat agtgtgtcac cagggccaga
      841 tgcccactgg agccgccgtc cctatcggcc tcgatccagg ttaagcagta cctgagaccc
      901 cgcagaaata ggggggtgac tgtagtacct cgcgaaagcg ctaccgcgca tgaagcgccg
      961 cagggttaag taaagtcgat agacgctctt taaaccgttg atttgctaag tgttccgatt
     1021 ccgctgggat cggatgcgag aagagaaacc tagtgctgcc aacactgggg aggcccgaca
     1081 gtcgccaact gtcgggctgc cactgccgaa gccgtgtgct cctaatcgag ctggcacggc
     1141 aaacgtaggg cgctccgggg tgaaatcccc cggttcgacc caatccactg gacgcccgaa
     1201 tgggcggggt tgggcgacac ggaaacgggc ctcgtggcga cacgataagc cctgtctgtt
     1261 ccgaacaggt ggaaatcctg gctgcgggct gctggagacg gtagaacgca gaccctgctg
     1321 gtgggctgct gagggcgttc gtaaggcgtc agggcgcgga ggggaaacct ggaagcgttg
     1381 gtactcgatg gggcaacctg ttgagatcgc tgtatagctc aagccagtaa ttgcggccgt
     1441 tgaggtcgca tcggggtaag aggcacacag ccatgacctt gcgggtctgg ccatcatgtt
     1501 gaaggacggc gctagtaagc gagctgcttt ggaatctcgg gccgctgaaa accctgctac
     1561 ggcaggtgtg aggtgtagac cagggcgtcg atctggagtg agtggcggtg tgttttgacg
     1621 taacagacta acgtgggaag cacctgggcg gatggacggt tggcactgtc caagccaggt
     1681 gtgggagagc tgttgagtag gcgcgaaagc tgctgggcga tatggtcgaa gcccagtggt
     1741 gaggcggaat aagggcaggt tggcacctgg ccccgctatg agctgaaaga cggcggtggt
     1801 ggcgttctct gggggtggca ccccacgcat agtgctcccc gttgcgatgg tatcgcggcg
     1861 gagcacttgg caaacacggt tttaagggca catgaccctg tgaagctcct gcacccttcg
     1921 gggtgcgggg gctttctcaa gcgcctggtt tcgggtgctt gagaaagcct atcccttccc
     1981 atcaggcctc taagcccgca gaagggcgtc tctgccttcc tccaagcatc tggcttggcc
     2041 aaacgaccaa ccggccatcc aggcgtctcg ctgagccgtt ttgagcgcat gtgggcacgt
     2101 tgatcggcca tggcctagcg gttgctctgc taagtgccac gcaacggcgt tatgcagccc
     2161 cgcgtgccac tctggcgact tgtcgctacc ccagcctaca gctgtcagca gcttgtcagc
     2221 gtggtcatgg tgccattgct caatcattac tttctccttc atttgttgat attggcaaag
     2281 gttgctggag gtgtgcatct ccctctgttt ttgattttgg ttgctgtctc tcaattactg
     2341 tcgtgaatgc aatccgattg ccgttgaacg cgaaattcgg gttgatgtaa taccagcctt
     2401 ggcgtaggtg tttagcgatt atctgagcct tttccagctc ggctagaccc cgccaaaaag
     2461 ttggctgaga tagcttgata ggcggctttc tatcggagtg ttctgctaaa aactcatcaa
     2521 gcacgatctt gtctagcggt attaaatctc gctcaaccgc tcggttctgc aagcaccaga
     2581 gcagcacgtt aaatgccttg ataccggctg ccttgaggtc aaaagtgaga gcaatattgg
     2641 cagtaaatag cttcacaaac tgctcagcat ctacacgtcg ataggtcgtt acatgggtac
     2701 cctgaacctc ccctgtagct tggtttacca ggatgttgtc gtccttccct agctgcgaca
     2761 gtctcacggc ttgccctctg atcggcacta tcatgttttc gataaatggg ttcttgtcgt
     2821 acctgacaac cttgctcata ttccgccctt aatctgatag accagcattc aaaaaattat
     2881 gtcggtgaat caaaaataac cactttattg atagccgtct atcattaatt atcttttttt
     2941 caatagaccg accaactagt tctgatagac cgactaaccc gttttgaaaa aacggctccc
     3001 gccccatgcg ggttcgttgg aaatcccttc ttatatcttg ttaagggggg ataagagcga
     3061 agcgaactac caacctgttt gagagtgcga tatgcactct gatcagtgca gccctgcaca
     3121 ctaacgccct cgttcctcgg ttgttgggca ccgccgttca cgaatcggag attcgtttca
     3181 cgacggatgc cagggagggg aattgttcca attttttaac ccatcaactg caaccccgtt
     3241 tagcaaggaa aggaggggta tccgcgatag cggatgcagc gacgaaggag cgggaacccg
     3301 ctgggcactg cactgagggc ctgcacgtgg gcttgggcgc cgctgggact aaaggcaccc
     3361 ccagtcgtgc taaaccgatg tttttgttgt tttggttctg gcggcgatgc tctcagcttt
     3421 gacggtctcg ccttgggtgt agcgacctag ccagccgatg tggagccgtt tatgtggcca
     3481 ctcgcctcgg ttcaggcgct tccatcgctt aactgcgctg ttctccaggg cgtacgcctg
     3541 tttcctggac tgagcaaatc ctggcacgcg ctgcaggctg tgtagggcct gccggagggc
     3601 atcgccgcca tcactaggcg cgttttgtac tgcgctggct aaacgggcct cccagcctgc
     3661 ataggaatcc gcagtgattc gccaggtcca cgcagggcta ttgcctgggc gcggggcttt
     3721 tagagcctca tagtcagcat ccggcatggt caatcgcccg cgtttgctgc cactatgctg
     3781 taggttttca aggtcgtggg ccatgccctc gccctctgta actaacagcc accaccagat
     3841 tttgccgggg ctggttttgt cgatcaggcc aaccaggtag ctattgcact ggccttttgc
     3901 tttgcgtcgc caccgttgtt gagctgttgc gtgaattaga taacggtcta aaaatttagc
     3961 ctcaagtgca ggccattttt tggcctcgat gctgccggct gtccagtaga tatatcccct
     4021 cgacaggtgc cgaagcaaaa gacgcatcgc gtctgttttg taccgaaaaa tcactggatg
     4081 attcccccca attttctgtt ttgcacgaac acctttatcg ctatatccta ctgagagtat
     4141 gtgagtaata tacctgccaa tgtagattgt gctatataag aatcttgcac aattgtagat
     4201 ttaggcacaa aga
//
