LOCUS       CP098530                4719 bp    DNA     circular BCT 12-JUL-2023
DEFINITION  Halomonas sp. KG2 plasmid pHaKG2_2, complete sequence.
ACCESSION   CP098530
VERSION     CP098530.1
DBLINK      BioProject: PRJNA846065
            BioSample: SAMN28867506
KEYWORDS    .
SOURCE      Halomonas sp. KG2
  ORGANISM  Halomonas sp. KG2
            Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
            Halomonadaceae; Halomonas.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Schmitz-Esser,S. and Schultz,D.L.
  TITLE     Complete genomes of cheese rind Halomonas and Pseudoalteromonas
            strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Schmitz-Esser,S. and Schultz,D.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2022) Department of Animal Science, Iowa State
            University, 3222 NSRIC, 1029 North University Boulevard, Ames, IA
            50011, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-AUG-2021
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 157x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/07/2022 17:07:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,082
            Genes (coding)                    :: 4,028
            CDSs (with protein)               :: 4,028
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 23 of 54
            Pseudo Genes (incomplete)         :: 34 of 54
            Pseudo Genes (internal stop)      :: 13 of 54
            Pseudo Genes (multiple problems)  :: 12 of 54
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Halomonas sp. KG2"
                     /mol_type="genomic DNA"
                     /strain="KG2"
                     /isolation_source="Cheese rind"
                     /db_xref="taxon:2951138"
                     /plasmid="pHaKG2_2"
                     /geo_loc_name="USA: Vermont"
                     /collection_date="2021-04"
     gene            complement(join(4055..4719,1..271))
                     /locus_tag="NDQ72_20765"
     CDS             complement(join(4055..4719,1..271))
                     /locus_tag="NDQ72_20765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002030349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WKD30510.1"
                     /translation="MTQPALACQGPSPTNLVLFRQRLPRKPYHTDNPRHGLLIRDVQRA
                     LSARYIQPNQPHRRYWLVYDVDRELAALDWNDLGAPPPTIVAQNPQNGHAHLIYGLDVP
                     VRTAPDAKSAPLRYAAAVDCALRAVLGADEDYAGLICKNPLHPHWRVTEWEPRLYELGD
                     LDSWLDLSPYADRRKRLPAYGLGRNCNLFEYLRTWAYRAIRQGWPEYGRWLEACLSRAE
                     GYNISEFSGTKAGKLPASEVKATAKSVARYTHKHFSQEGFSQWQARNGRKGGIASGKAR
                     RQGSEAESQPWAVLGVSRRTYYNRKRLGKL"
                     /name="replication initiation protein"
     gene            complement(405..671)
                     /locus_tag="NDQ72_20770"
     CDS             complement(405..671)
                     /locus_tag="NDQ72_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WKD30511.1"
                     /translation="MVWQIELTDTAAKQLKKIGHTEAKRIRNYLRERVQPLEDPRQLGK
                     PLQGNMGELWRYRVGDYRLVAQIENDRVCVLVVRIGHRKEVYR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(655..879)
                     /locus_tag="NDQ72_20775"
     CDS             complement(655..879)
                     /locus_tag="NDQ72_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WKD30512.1"
                     /translation="MLAIRLPSEIEMRLEALAKATGRTKTYYAREAILEHLEDLEDIYL
                     AEQTLERLRSGDEVTHSLDDVERELGLAN"
                     /name="DUF6290 family protein"
     gene            complement(945..1214)
                     /locus_tag="NDQ72_20780"
     CDS             complement(945..1214)
                     /locus_tag="NDQ72_20780"
                     /inference="COORDINATES: protein motif:HMM:NF016769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKD30513.1"
                     /translation="MGERLRELANHVRGNQPGQPGPERAGEALKRAGGGLERSERQQDR
                     TIERAGVALGSAGRGVEREREALDARGRESERVKSRQRGHGFGI"
                     /name="hypothetical protein"
     gene            complement(1157..1288)
                     /locus_tag="NDQ72_20785"
                     /pseudo=""
     CDS             complement(1157..1288)
                     /locus_tag="NDQ72_20785"
                     /inference="COORDINATES: protein motif:HMM:NF016769.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1794..2225)
                     /locus_tag="NDQ72_20790"
                     /pseudo=""
     CDS             complement(1794..2225)
                     /locus_tag="NDQ72_20790"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2441..2779)
                     /locus_tag="NDQ72_20795"
     CDS             complement(2441..2779)
                     /locus_tag="NDQ72_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011178376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WKD30508.1"
                     /translation="MADAKPKREKIIKVRVSAEEHETLKWRCPKAQLAEWMREQCLNPD
                     GDWVRQSKGPDPVDPALLRQLAGIGNNINQIARRVNSGEWGAVDRVAVIAALSAVEREL
                     SELRGQYR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3138..3338
                     /locus_tag="NDQ72_20800"
     CDS             3138..3338
                     /locus_tag="NDQ72_20800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKD30509.1"
                     /translation="MSNAGDQLRLAIQESQGKAGKGDKRVPVEVPPDIHAALKSLAARE
                     GTTMRALALEGLAHIFDKYDR"
                     /name="hypothetical protein"
ORIGIN
        1 gtggattttg ggccacgatc gttggcggcg gggcaccaag atcattccaa tcaagcgccg
       61 ctagctcacg gtctacgtca tagaccagcc agtaacggcg gtgcggttga tttggctgta
      121 tgtaccgcgc agagagggcc cgctgcacat cacgaatgag caacccatgg cgaggattgt
      181 cggtatggta tggcttccga gggagccgct ggcgaaaaag caccagattt gttgggcttg
      241 ggccttggca ggccagtgct ggttgggtca caatatcctc acttactgca ccctcgttcc
      301 cgtcatgccg ccaagctaat ccgggttcgg gggtgtttcc ttttgggcac tctgctgagt
      361 gctattcctt tgtttttact cttaagaacc gccgccgtcc agcctcagcg gtagacttcc
      421 ttccgatgcc cgatgcgaac aacgagcacg cacacacgat cattttcgat ctgtgcaacc
      481 aaccgataat caccgacgcg gtaccgccac agttcgccca tattgccttg cagaggcttc
      541 ccaagctgcc tggggtcttc cagcggctgt acgcgctctc tcaggtaatt acggatgcgt
      601 ttagcctctg tgtggcctat ttttttcagt tgcttagccg ccgtatccgt cagctcaatt
      661 tgccagacca agctcacgct ccacatcgtc cagtgagtga gtaacctcat ctccgctgcg
      721 cagtcgttcc agggtctgtt cagccagata aatgtcctca aggtcttcca gatgttcgag
      781 gatcgcttct cgtgcgtaat aggtctttgt gcgtccagta gccttggcaa gggcttctag
      841 gcgcatctca atttcgctgg gcagtcgaat ggccaacatg gcaggtctct ccaaacattg
      901 atatacacgt atagcatatg cctattttag atctatcact agcgtcatat gccaaaacca
      961 tggccccgct gccgggattt caccctctcc gattcccgtc ctctggcgtc caacgcttca
     1021 cgctcgcgct caacccctcg acctgctgag ccaagcgcaa cacctgcccg ctcaattgtt
     1081 ctgtcctgct gtctctcgct tcgctcaagg ccgccacctg cccgcttaag cgcttcacct
     1141 gctcgctcag ggcccggttg tcccggctgg ttgcctcgaa catggtttgc aagctctcgt
     1201 aagcgctctc ccacgccttt tgctgccttt cgtagtcgct ctgtagttgc tcgaaggcgc
     1261 gcaatagctc gcgttccaag ttggtcatct agtacccctt caggattcga tattggccat
     1321 tctcgccgaa cgcgtccggc ttctccccga gactgtccgg gtggatctcg gcgcacagcc
     1381 tcctgctgtc cccgcatgtc gtcaggttga tatcccctcc ctgttcgctc aaacggctta
     1441 tcgtggcgtt ctgctggctg atcgtgtcga cctggttctt gatgtgccag cccaacgccc
     1501 agatggcccc cgcgctcagt agcaacgtca cgatcacggg aatggctacc gccatccagc
     1561 tcctgagcgc gatccgctct aaattctgcg actgggcgcg gatggcgttt ttggttctct
     1621 gctcgctttc gctcaacgcc gacagtaagg cgctctcgtg cttctcgaag gcgctcacta
     1681 cggccttctc ggtactcgcg gcccgctctt tcgatctctc ctcgaaggct gtcgccaact
     1741 tcgaaatctc gctcatacag ctttcctcgc agtcgcatag gcttgccgcc ttccgggtcg
     1801 gcaatgctga tgctggtctt ggtttggcgc gtcacttcaa aacctgcccc ctcaagggcc
     1861 gccaccactt cggcgcggtt tgtcacctcg cctttttcag ccaacgccaa aagcccatca
     1921 gtaacctggc gttgtgcttt ttccttgtct cgcggcaggt tagcggggta cgtaagggcc
     1981 ctctggttgg cagggtcgct agggtcatgc aggttgtatt tggcgttcgt tagggtcttc
     2041 caagcatcaa ctcggggacg gtcggcgcgg tcgtagtagg gctgtaatcg cctcccagta
     2101 gtcagctcgg tattcgggat gacgaaattc agctccaagc ggtctttatc ccggtgctca
     2161 acccacaggc agtcgtattg gtcagcatcg aggccgggca tcagggccct ctcaaaggtg
     2221 tccatgattt gacgcttatg ggcatcggga agatcagcct ccgcaaacga cagaacgccg
     2281 ctggtgtagg tcttggcata gctgctggcg tcgatcagct cccttgtttg ctcggggtcg
     2341 cctcgcagcg tttcggcacc ttcccggtcg cggtccttgc ctagcaggta atcgaccggc
     2401 ccagagccac taccagcgcc tctggcgtga aatttcacta tcaccggtac tgacccctta
     2461 gttcggacag ttcgcgctct acggcgctca gagcggcgat aacggccact ctgtccactg
     2521 ctccccattc gccgctgttc actcggcggg caatctggtt gatgttgttc ccgattcccg
     2581 ccagttggcg caatagcgct gggtctacgg ggtcagggcc ctttgactgc ctcacccagt
     2641 caccatcagg gtttagacac tgctcgcgca tccactcggc gagctgggcc ttggggcaac
     2701 gccatttcaa cgtctcatgc tcctcggcag agacccgaac cttgatgatc ttctcgcgct
     2761 tcggtttggc gtcagccata gccgcctcct tggtttttca catggccacc gcgcagagcg
     2821 tgcccgcttc cagcgggtgc gatatgggcg gtggctacgg ggggtgttgg ggggcggtag
     2881 ccccctgacc cagctcacag aggcccagac gagcgaagcg agtcaaaggg ccctctgtgt
     2941 atgctggctt atgtctgtgg aatcgtcaac aggtgggggt tagtttcagg atcagtggca
     3001 ctacgctgac gcctcgctct gctcgttgtc ggacaccgca tctcacggat caaagatccg
     3061 tttcgctacg gatgtcagga gtgataatgt gctaatcttt catttgtact taaacacaaa
     3121 cacacatagg taactgcatg agcaacgccg gtgaccagct tagattggcg atacaggaat
     3181 cacaggggaa ggcaggcaaa ggggataagc gtgtgccagt ggaagtgcca ccagatattc
     3241 atgcagcgtt gaaatcacta gctgcgcggg aaggcaccac catgagggct ctcgcactgg
     3301 aggggctagc tcacatcttt gataaatacg accgctaaac cccgccctga cgggctgtta
     3361 tctgatatgg cttcgagtgc aacttaagaa cgattctcac agcccatatg cgctacgctg
     3421 tgtgctgttt gtcatgatta aggcctgtct atgtacctca aatatgttct ttcctgccta
     3481 gcgctaggca gcattgctgc caatatcagc gcttgtgcca gtacaggcgt cgactcagag
     3541 ccacgcctgt atttcaaatc ccatgctaat gagtttgaaa taaccccaaa ttgtttacgc
     3601 agcgtttcaa tgagcgaaga tggagatggg cgagacttca tcactgtgga ctttattcga
     3661 tcagaaggtt gctatggcaa gctcgatacc tgggtgcgag ggaacattgg ggaggatgtg
     3721 agattgatct tcaatgggga aactgtatct gggccgagtc tagttgcagg gcccttagcg
     3781 gctgacaacg tttggatagg gtctgaaaat agcgatcttt tatcagatgt ttacaagcat
     3841 ctgtccctat ctagatagct taggccagac ttcaaccgta catgctcaac acgctccgcg
     3901 acttttgcag ccatttgttc tgctgtagcc ctggggagca tgatccagtc ccgggctaac
     3961 agccaatcta acgctgcctg aaaagggtct ttatctgctt gtgcaaaggc atattccttc
     4021 gcacccatgt aaagaatttc ctgctcgtaa gggttcacag ctttcctagc ctcttccggt
     4081 tgtagtaagt gcggcgggaa acacctaaaa cggcccatgg ttggctttca gcttctgacc
     4141 cttgccgacg ggccttgcca gacgcaatgc ctcccttgcg tccattccga gcttgccact
     4201 ggctaaatcc ttcctggctg aaatgcttgt gggtgtaccg ggcaacgctc ttggccgtcg
     4261 ctttcacttc gctagccggc aattttccag ccttggtgcc cgaaaactcg gatatgttat
     4321 agccctcagc acgacttagg catgcttcaa gccatcgtcc gtactccggc cacccctgcc
     4381 gaatggctcg ataagcccac gtccggaggt attcaaacaa gttgcagttc cggcctagcc
     4441 cgtaggccgg aagccgctta cggcggtctg cgtaggggga gagatcaagc cagctatcaa
     4501 ggtcgcccaa ctcgtacaac cgcggctccc actcagtaac acgccaatgg gggtgtaaag
     4561 ggtttttgca gatcaggccc gcatagtcct catcggcccc caacacagct cgtagcgcgc
     4621 aatctacggc ggcggcgtac cgcaacggcg cagatttcgc gtctggtgcc gttctaacag
     4681 gcacatcgag gccatagatc aggtgagcat gcccatttt
//
