LOCUS       CP097476                5596 bp    DNA     circular BCT 03-AUG-2023
DEFINITION  Klebsiella pneumoniae strain 16HN-12 plasmid p16HN-12_7, complete
            sequence.
ACCESSION   CP097476
VERSION     CP097476.1
DBLINK      BioProject: PRJNA503173
            BioSample: SAMN10353622
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Yang,X.
  TITLE     A multi-drug resistant plasmid mediates transmission of virulence
            plasmid in Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2018) The Department of Applied Biology and
            Chemical Technology, The Hong Kong Polytechnic University, Hung Hom,
            Kowloon, Hong Kong 999999, Hong Kong
REFERENCE   3  (bases 1 to 5596)
  AUTHORS   Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) Department of Infectious Diseases and Public
            Health, City University of Hong Kong, Kowloon Tong, Hong Kong
            999999, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina NextSeq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/19/2022 10:53:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,972
            CDSs (total)                      :: 5,855
            Genes (coding)                    :: 5,678
            CDSs (with protein)               :: 5,678
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 64 of 177
            Pseudo Genes (incomplete)         :: 122 of 177
            Pseudo Genes (internal stop)      :: 25 of 177
            Pseudo Genes (multiple problems)  :: 32 of 177
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="16HN-12"
                     /isolation_source="pus"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p16HN-12_7"
                     /geo_loc_name="China"
                     /collection_date="2016"
     gene            complement(join(5475..5596,1..424))
                     /locus_tag="ELM62_29185"
     CDS             complement(join(5475..5596,1..424))
                     /locus_tag="ELM62_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKV69921.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(450..776)
                     /locus_tag="ELM62_29190"
     CDS             complement(450..776)
                     /locus_tag="ELM62_29190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKV69922.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1932..2108
                     /locus_tag="ELM62_29195"
     CDS             1932..2108
                     /locus_tag="ELM62_29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKV69923.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2098..2442
                     /locus_tag="ELM62_29200"
     CDS             2098..2442
                     /locus_tag="ELM62_29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WKV69924.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2651..3085
                     /locus_tag="ELM62_29205"
                     /pseudo=""
     CDS             2651..3085
                     /locus_tag="ELM62_29205"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3120..3629
                     /locus_tag="ELM62_29210"
     CDS             3120..3629
                     /locus_tag="ELM62_29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WKV69916.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            3636..3848
                     /locus_tag="ELM62_29215"
     CDS             3636..3848
                     /locus_tag="ELM62_29215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WKV69917.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4515..4739
                     /locus_tag="ELM62_29220"
     CDS             4515..4739
                     /locus_tag="ELM62_29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WKV69918.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4723..4989
                     /locus_tag="ELM62_29225"
     CDS             4723..4989
                     /locus_tag="ELM62_29225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WKV69919.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5059..5409)
                     /locus_tag="ELM62_29230"
     CDS             complement(5059..5409)
                     /locus_tag="ELM62_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKV69920.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     Typing          1767..1846
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(1184..1581)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2433..3964
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgggagctac tctatataac atataacctg agttaagggt ttcttttata ctttcttcaa
       61 tgctcattat aaatattagt ggattatcat ttttcttccc ccatgggttt ctaattaatc
      121 cttcgatgta ctcaggtcct ttgcgtccta ttgcctgcgc aatatttttt atttcatctg
      181 cgtgagggct ttgttgtaca gctttgttta tgcctgcttg agtgattttc agagactcta
      241 caccgtgtat gttgtatgag ataaaatgta ataaattaag atgcaatgca attaatccat
      301 ttttgtttac atctgagtat ttggataaaa aacgtaatga agtgttaact ctcccttgaa
      361 gcctaacaat tgggagtgct gccttgtata tttccttctc cgttaaatac cgtttaaaaa
      421 acataattct ccgcctccgt atagcgtgcc tattttattt cgtcgtaaaa cttagctctg
      481 aaatattcct ttagaaccat catgtcatta ataattgaat ctatctcatc cattttttct
      541 tggtattctt ttaatggtga agggagcggt aactcaatat ttgaaaagtg attggcgaga
      601 tagggatcaa ggtttctcat ccattcaggc ttttcgccaa tgcgttcttc aactgaaagt
      661 gaacctgata atctctggtt aataagatca cttagactca ttttggtttt attcgaaagc
      721 cgttgtaagg attgctttaa tcctatatct agcatgatat ttgttctttc tctcattacc
      781 cccccttgct attgcgtttt ttgttgcgca caaagctacc attgcgcatt aagaaacgca
      841 ataaaaaagg agggagggag tggtttgtac atgatgtgca gtgcgctgag ggcatcagtg
      901 gcgggtgttt ttccgtggga tggatggagc tccggggata acagaactga cgatcattta
      961 aaggatcttc ttgagatcct ttttttctgc gcgtaatctc ttgcactgta aacgaaaaaa
     1021 ccgcctgggg aggcggtttg atcgaaggtt aagtcagttg gggaactgct taaccgggta
     1081 actgggtata cgagaccgca gataccaaaa ctgttctttc agtgtagccg cagttaggca
     1141 cccacttcaa gacctctcaa tatctctctc gtatatatcc agttaccaat ggctgctgcc
     1201 agtggcgttt ttgcgtgtcc gtccgggttg gactcaagat gatagttacc ggaaagggcg
     1261 cagcagtcgg gctgaacggg gggttcgtgc atacagccca gcttggagcg aacgacctaa
     1321 accgagccga gataccagca gcgtgagcta tgagaaagcg ccacgcttcc cgaaagggaa
     1381 aaaggcggaa caggtatccg gtaaacggca gggtcggaac aggagagcgc acgagggagc
     1441 cgccggagag aaatcaccgg catctttaag tcctgtcggg tttcgtcctc ctctgacttg
     1501 agcgtccatt tctgtgatgc tcgtcagggg aggcggagcc tatggaaaaa cggcaacgcc
     1561 gcatcctttt ctccggccct ttctgatttg agcgcctttg ctttatcccg ttatccgtga
     1621 gtcggtcacg cccgccgcag ccgaacggca ggagcgcagc taccgagtga gcgaggaggc
     1681 gtcatttcat ctgtccgtgc atggtattgc ggacgctcct gagccacata tcacacctgc
     1741 cacatgaggc actttcatga atcgtgctct catgccaaca tagtaagcca gtatacacag
     1801 cgtaaacgct gtgactggtt cagggctgcg ccccgaaacc cgctaacacc actgacgcgc
     1861 tggcgcttgt ccaacgccgg agcgacccgg aaaacttttc cgtgccgtcc ggcgtttgcg
     1921 aaggagggat aatgcagaag ccgaagaaga agccgctcgt cgtaaaaaaa agatcctggc
     1981 atccggcagt gatggcaaat ctgatgcgtc aggcacagga ggaagattac cgccgggagc
     2041 agatggcaga gcttgagcgt tcagcccggt cacattttgc ggatatcgga ggagaggatg
     2101 gcggttaagc gtacccgttt tttaggtatc cgggtgaccg atggggagta ccagcagctg
     2161 ctggagcgct gcaatggaag gcaactggcg gtgtggatgc gggaaacctg tctcgatacg
     2221 cgcccggcgc ggtcgttgcg gctgccgtcc atcgatccgg ttttgctgcg ccagcttgcc
     2281 gggatgggga acaacctcaa ccagatcgcc cggaaaatta acggcggcca gtggtcaggc
     2341 gctgaccggg ttcaggtggt ggccgcgctg atggccattg atgccgaact cgagcggctg
     2401 cggcatgccg tgctggaaaa gggggcagac gatgatcgtt aagtttcatc cgcgagggcg
     2461 aggcggcggt gccgggccgg tggattatct gctggggaaa gaccgccagc gcgacggggc
     2521 catcgtcctg cagggaaagc cggaggaggt ccgggagctt atcgacgcct cgccctacgc
     2581 caaaaagtac acctccggcg tcctgtcttt tgctgaacag gatttaccgc ccggccagcg
     2641 tgaaaagctg atggcgagct tcgagcgggt tctgatgccc ggactcgata aagaccagta
     2701 cagcgtgctg tgggttgaac accgggacaa ggggcggctg gaactgaatt ttctggtacc
     2761 gaacacggaa ctgctgaccg gcaggcgtct ccagccgtac tacgaccggg cagaccgtcc
     2821 gcgcatcgat gcgtggcaga ccgtggtgaa cgggcgactg gggctgcacg acccgaacgc
     2881 accggagaac cggcgcgcgc tggtgacgcc atcttcgtta cccaaaacga agcaggaagc
     2941 cgcagaggct attacgcggg gcttactggc cctcgcctcg tcaggggagc ttaaaacgcg
     3001 tcaggacgtc actgaggcgc tggaaagcgc aggttttgag gtcgtgcgca ccacaaagag
     3061 cagcatcagc attgccgacc cggacggagg gcgaaacatc cgacttaagg gagccatcta
     3121 tgaacagtct tttaacgctg gcgaaggact tagagcagaa atcgaaagcg cagcagcaga
     3181 gtaccggcga aatgctgaaa gccgcattca gcgagcacga gaagtctgtc agagcggaac
     3241 tgagcgaaag cgggaagaga atcagcgccg ccatccgcgc ccacgagcag ggtatgagcg
     3301 cggccatgca gtcgaaccgc ctgagcgtga tgcgtatggt cgggcggaca tggctgacca
     3361 tcacgatggt gtcaggactg ctgttcgcca gcctgagcgg ggtgttgtgg tatcagggga
     3421 gcttgatagc gtcaaatctg gccgaaatcg accggcagaa cgcagcgctg tcgaagctga
     3481 acgcgaagac ctggggcgtg acgtacctgg aagacagcaa cggacgtttt ctggtgctgc
     3541 cgaaggggac ggccgtagac aggacgcaga gctggacggt cgggaacggg cggtcgaagc
     3601 agaacgcgct gagactggtg aaggagtaag tgccgatgac cgagctggaa aaacagttgc
     3661 tgagcgcatt agagcagcta cagcaggact actcgaaaag gctggacgag tgggagagcg
     3721 ccttcgggga atggcggaaa atgtcagggc ttatgcaacg ggagaacgcg gcgctgagcg
     3781 agcgcgtgac gcgcttgagt cagcaggtgg aacgcttgag cgggcaactg cagcgtttga
     3841 gccggtagtg cagcgtcatg agatggccgt ggccgcagaa cgggcgcacg agcaacggca
     3901 gcacgaaaag gaactgacgg aagcgcgttc ccggcagcga agctatgacg gtccgtcgct
     3961 gggctgaaat gcaggaaaac agggttctca tcgggcattt acgctgaaac cggatgtcat
     4021 tcacctggct gcatggctat gcagccaggt aaaatttatc tgtcgcgaac ggggcggttt
     4081 atcgggtggt ttgttgccgg ttttacctga ctgccacccc gttcgcggcg aacccgtccg
     4141 gggcggtgcg ggcaacgggt gttatgttta atcagcgtga acggcagata caaaaaagcc
     4201 ccggagggct tttaactact gacttatttt tttgaagatt tgctgggttt caatttgtca
     4261 gtatttcccg gcgttttttt attgtcacgc tggcgtttat caggttcccc tgttgaatcg
     4321 gcaattcgtt tcggacccgg cttaagaccc ataatcgtta tcctttcaat agttaagagg
     4381 aaagccctca agctagatat attgaccgcg ttacggatta tcaagagggg ctaacaagat
     4441 tattttttgt tcttttaggt tgattgtggt actgttgata tacatgtata gcaaatggtg
     4501 ttcgggaggt ttatatgctt gctatccggt tatctgatga gattgagtcc cgtctggact
     4561 cgctggcgaa gcaaaccggc agaacaaaga cgttttatgc gcgggaagca atactggcgc
     4621 atctggaaga cctggaggat tattatcttt cagcagaaac tgctgcacgc gttcgccgtg
     4681 gtgatgaagc agtgcattcg tctgaagacg tgaggaagtc acttggtctg gacgattaac
     4741 tattccgatc gggcgctcaa atcgttacgc aagatggaca aacagaacgc acgacggatt
     4801 gtggatttta tgagtttacg cattgcagtt gctgccgatc ctcgccagtc agggaagccg
     4861 ctcaaaggtg agctgggcga gttctggcgc tatcgggtgg gagattatcg cgttctgtgt
     4921 gagatccgag atgacgagct tgttatcctt gccgccacga ttggacatcg ccgcgaagtt
     4981 tacgactgaa gcccgctatg cgggcttttt tgatgccgac aacaggtatt atggtaaata
     5041 ctcgactaac tacgtgttct actgtttgtc ggttttagtt aaccagcagg ttgtcttaac
     5101 gataatgtag ccgttatttt taactaataa ggtcatattg ttaacgtcag gcataataaa
     5161 agacattatg gcctcagagt tatcatcaga agcagctctc tgcattacta cttcgattcc
     5221 cttttctttg aatctataag tatccagttg cctattaaac ttagaaagac ctacaaccat
     5281 acttagtttg ttatctttaa cagtaagaga tgcttcctga gtaccattac atcggtagta
     5341 agcggtgtaa ccgtcagcaa aagatcgacc ggggaatata aatgctagga acgcaattaa
     5401 ccaatacata gttgagaatc ttggtattac acatttcact atcctctctt tcatttttcg
     5461 ttcaccatac tttgttatct gatttttaat ttttctattt tataaaccaa gtctctacca
     5521 aaaaggcgaa aattatcctc aaataataat aattgtttaa gaacatccat gaaagcagct
     5581 gcttcaccct cagtta
//
