LOCUS       CP097475               11970 bp    DNA     circular BCT 03-AUG-2023
DEFINITION  Klebsiella pneumoniae strain 16HN-12 plasmid p16HN-12_6, complete
            sequence.
ACCESSION   CP097475
VERSION     CP097475.1
DBLINK      BioProject: PRJNA503173
            BioSample: SAMN10353622
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Yang,X.
  TITLE     A multi-drug resistant plasmid mediates transmission of virulence
            plasmid in Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2018) The Department of Applied Biology and
            Chemical Technology, The Hong Kong Polytechnic University, Hung Hom,
            Kowloon, Hong Kong 999999, Hong Kong
REFERENCE   3  (bases 1 to 11970)
  AUTHORS   Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) Department of Infectious Diseases and Public
            Health, City University of Hong Kong, Kowloon Tong, Hong Kong
            999999, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina NextSeq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/19/2022 10:53:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,972
            CDSs (total)                      :: 5,855
            Genes (coding)                    :: 5,678
            CDSs (with protein)               :: 5,678
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 64 of 177
            Pseudo Genes (incomplete)         :: 122 of 177
            Pseudo Genes (internal stop)      :: 25 of 177
            Pseudo Genes (multiple problems)  :: 32 of 177
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="16HN-12"
                     /isolation_source="pus"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p16HN-12_6"
                     /geo_loc_name="China"
                     /collection_date="2016"
     gene            103..1788
                     /locus_tag="ELM62_29115"
     CDS             103..1788
                     /locus_tag="ELM62_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003723 - RNA binding [Evidence IEA]; GO_function:
                     GO:0016788 - hydrolase activity, acting on ester bonds
                     [Evidence IEA]; GO_function: GO:0043022 - ribosome binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="WKV69911.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            1798..2055
                     /locus_tag="ELM62_29120"
     CDS             1798..2055
                     /locus_tag="ELM62_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WKV69912.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="cloacin immunity family protein"
     gene            2140..2289
                     /locus_tag="ELM62_29125"
     CDS             2140..2289
                     /locus_tag="ELM62_29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WKV69913.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3773..5737
                     /locus_tag="ELM62_29130"
     CDS             3773..5737
                     /locus_tag="ELM62_29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WKV69914.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5737..6468
                     /locus_tag="ELM62_29135"
     CDS             5737..6468
                     /locus_tag="ELM62_29135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WKV69915.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            6475..7005
                     /locus_tag="ELM62_29140"
     CDS             6475..7005
                     /locus_tag="ELM62_29140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKV69902.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7032..7211)
                     /locus_tag="ELM62_29145"
     CDS             complement(7032..7211)
                     /locus_tag="ELM62_29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WKV69903.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7237..7665)
                     /locus_tag="ELM62_29150"
     CDS             complement(7237..7665)
                     /locus_tag="ELM62_29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKV69904.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7829..8143)
                     /locus_tag="ELM62_29155"
     CDS             complement(7829..8143)
                     /locus_tag="ELM62_29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WKV69905.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8131..8409)
                     /locus_tag="ELM62_29160"
     CDS             complement(8131..8409)
                     /locus_tag="ELM62_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WKV69906.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(8584..8949)
                     /locus_tag="ELM62_29165"
     CDS             complement(8584..8949)
                     /locus_tag="ELM62_29165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015291 - secondary active transmembrane transporter
                     activity [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WKV69907.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8946..9317)
                     /gene="tolA"
                     /locus_tag="ELM62_29170"
     CDS             complement(8946..9317)
                     /gene="tolA"
                     /locus_tag="ELM62_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0031237 - intrinsic component of periplasmic side of
                     plasma membrane [Evidence IEA]; GO_function: GO:0005215 -
                     transporter activity [Evidence IEA]; GO_process: GO:0006810
                     - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WKV69908.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(9591..9836)
                     /locus_tag="ELM62_29175"
     CDS             complement(9591..9836)
                     /locus_tag="ELM62_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKV69909.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            10481..11968
                     /gene="ltrA"
                     /locus_tag="ELM62_29180"
     CDS             10481..11968
                     /gene="ltrA"
                     /locus_tag="ELM62_29180"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003964 - RNA-directed DNA polymerase activity [Evidence
                     IEA]; GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WKV69910.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             1..6788
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2393..2971)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5738..6465
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 cgcgcacgcc gggatctgtg cggggggtat ccggtaacgg gtatccctac cgcgacatgt
       61 tggctttaat ttattgtttt aaatgtcaaa gaggaaaacg gtatgagtgg tggagacggt
      121 cgaggtccgg gtaattcagg tctgggacat aatggtggtc aggccagtgg gaatgtgaac
      181 ggtacgtctg gtaaaggtgg cccttcatca ggtggtggta cggatccaaa cagcgggccg
      241 ggctggggta cgacgcatac gccgaatgga gatattcata actataatcc gggggagttt
      301 ggtaacggcg ggagtaaacc cggtggtaat ggtggtaaca gcggcaatca tagcggtagc
      361 tctggtggcg gacagtcttc ggccactgcg atggccttcg gtctgcctgc cctggctact
      421 ccgggggctg aaggactggc tttatcagtt tccggcgatg cgttgtcggc cgccgttgct
      481 gatgtgctgg ctgccctgaa ggggccgttt aagtttggtc tgtgggggat tgcgatctac
      541 ggtgtgctgc cttctgagat tgcaaaagat gatccgaaaa tgatgtcaaa aattatgacg
      601 tcattaccgg ccgatgcggt gacggagact ccggcaagta ctttaccact ggaccaggcg
      661 acggttcgtg tcagacaacg ggttgtggat gtggtgaagg atgagcggca gcatattgcg
      721 gttgtcgcag gtcggccaat gagtgtccct gtggtggatg cgaaaccgac aaaacgtccg
      781 ggggtattca gtgtgtcgat tccgggtctc ccgtctctgc aggtgagcgt accgaaaggt
      841 gttccgacag cgaaagcccc gccaaaaggc attgttgctg aaaaaggtga ttcacgtccg
      901 gctggtttta cagccggtgg taactcccgt gaggccgtta ttcgtttccc gaaagagacc
      961 ggacagaagc cggtttatgt gtcggtgaca gatgttctta ccccggcaca ggtaaaacag
     1021 cgtcaggagg aagaaaagcg tcgccagcag gcatgggacg ccgctcatcc ggaagagggg
     1081 ctgaaaagag actatgataa agcgaaagct gagctggatg ccgaagataa aaatattgcg
     1141 accttaaaca gccgcattgc atcgacagag aaggcgctcc ccggtgcaag ggctgctgtt
     1201 caggaagccg ataaaaaggt aaaagaggca gaggcaaata aagatgattt tgtgacttat
     1261 aacccgcctc atgaatatgg ctccgggtgg caggatcagg ttcgctatct tgataaggat
     1321 attcagaatc agaatgagaa attaaaagcg gctcaggcat ctttaaacgc aatgaatgaa
     1381 tccttatcca gagataaggc tgcacttacc ggggcgatgg agagccggaa acaaaaggag
     1441 aaaaaagcga aggatgcaga aaataagtta aatgaggaaa agaacaagcc tcgcaagggg
     1501 actaaagact acggccatga ttatcatcca gtacctaaaa cagaagacat aaagggactt
     1561 ggagagttaa agcgaggtga tcctaaaaca cctaaacaag gtggtggtgg taagcgggcc
     1621 cgatggtatg gcgataaaaa acgtaaaatt tatgaatggg attcccagca cggtgagctt
     1681 gaagggtatc gtgccagtga tggcgaacac ctaggcgcat ttgatcccaa aacgggtaag
     1741 caggttaaag ggccagatcc aaaacggaac attaaaaaat atctttgaga ggtaaatatg
     1801 gggcttaaat tacgtttgac atggtttgat aagaaaactg aagagtttaa aggggaagag
     1861 tattctaaag actttggtga cgatggttct gtcattgaaa gcttgggaat gcctttaaag
     1921 gataatatta ataatggtga gtttgatttg aagaaagaat gggttccttt actacagcca
     1981 ttttttaaaa atcaaataaa acctgatgtg gataattgtt atatagcttt tgattatcgg
     2041 gacggtaact ggtaacaaaa aattatcaag gatgatttga aatactgact tccggtgttc
     2101 agggatgaat gccggagtct ttaaatacag ggaaagtata tgaaaaaggt aaagactatt
     2161 tttcttttta tattgattgt tgctggcttt cttctggccg catgtcaggc aaattatatc
     2221 cgggatgttc agggcggaac ggtggcacca tcgtcctcct ctgaactgac ggggatcgcg
     2281 gttcagtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctttt
     2341 gccctgtaaa cgaaaaaacc acctggggag gtggtttgat cgaaggttaa gtcagttggg
     2401 gaactgctta accgtggtaa ctggctttcg cagagcacag caaccaaatc tgtccttcca
     2461 gtgtagccgg actttggcgc acacttcaag agcaaccgcg tgtttagcta aacaaatcct
     2521 ctgcgaactc ccagttacca atggctgctg ccagtggcgt tttgcgtgct ttcccgggtt
     2581 ggactcaagt gaacagttac cggataaggc gcagcagtcg ggctgaacgg ggagttcttg
     2641 cttacagccc agcttggagc gaacgaccta caccgagccg agataccagt gtgtgagcta
     2701 tgagaaagcg ccacacttcc cgcaagggag aacggcggac aggtatccgg taaacggcag
     2761 ggtcggaaca ggagagcgca agagggagcg acctaccgga aacggtgggg atctttaagt
     2821 cctgtcgggt ttcgcccgta ctgtcagatt catggttgag cctcacggct cccacagatg
     2881 caccgaaaaa gcgtctgttt atgtgaactc tggcaggagg gcggagccta tggaaaaacg
     2941 ccaccggcgc ggccctgccg ttttgcctca catgttagtc ccctgcttat ccacggaatc
     3001 tgtgggtaac tttgtatgtg tccgcagcgc ccgccgcagt ctcacgcccg gagcgtagcg
     3061 accgagtgag cgaggaggcg caatttcccc ggcacctgca agacggtcac cggacgcgct
     3121 gatgtgggat attgtcccct gattaagtct ttctattccc tgacgatcaa ttatcaggat
     3181 aggtgaacca tgtcggtttt tcgctgaaca ttttacgtta acctatctct gttgcttttt
     3241 tctccttgcg gagaaatgca agtcatcccc gctggggcgt ggctgacttc aggggaaaag
     3301 accttgctta cgctggctgg acgccaccga cgcacggtct tttcccctct ttctggctct
     3361 gtccggctgg acgccggcca cgccagaaat tcacccggcc cccgtcaggc tcacgcctga
     3421 ccacccacaa aaatccacgg tgctggacgc acctgacgaa gcagcaaagc tgctcctgat
     3481 tttgcgggtg gtcagccgct cgctatggcg aactctgacg ggggccggaa caccggaaaa
     3541 gaccgcactg gggtgccgcc ttttcctctc acacgctggg gcgtgctcgt tgttcctccg
     3601 gggctgcgcc ttttccgcat ggggcggaaa gaggctggcc tccgcggtga aagctgaacg
     3661 caatcaccac ggacgtgacc gccgccgcca tgccttgggg catgtccgcg gcttgtgcag
     3721 cgggggctgc gcatcacgcc ggtaaacccg tcgtctgaag gagagagatt gcatgtttaa
     3781 tacggattcg cttgcctggc agtggcatat cccggagctg gcccggcaga tttacccgtg
     3841 ggtcagctgg ctgtaccggg agtattacag tctggccggt ttttttctgg ttatcggcct
     3901 gtttttcggt ctggctgaac tctgggccgg ccgaatgcgt tttgtggggc tgagccataa
     3961 ggcgcggcgg gagtatgccg atcgctataa gcgcgataat aaccggactg tgccattttc
     4021 ggtgctggcc tcgctggatt cacccgggcg gatggtcagg gcacggcgac atgtctggaa
     4081 ctacgcgtac tggctgaccg gcgctgccgg tctggccctt ctgggggggc tggttgtcgc
     4141 cgtgtttatc gagagctggg gagaaaccgt cctgcgcccg gacctctatg cgaacgcaga
     4201 ttattacctg ctgcagccgg ctaacctgtt cgggatagcc tggtgtgccg gagggcttgt
     4261 cgccggcgtc cttctggcgc ggctgctggc gttcctgctg cttgatggtc attttgcggc
     4321 cgctgacgag gcggtgaacg cgaaactgaa tcaggagagc cggcgcagca gccagcggac
     4381 cggagaaatg acagacgtcc gccacctgca tttcggggag ccggtaccgg ttaacgcgct
     4441 ggccgatttt tccacggagc aggcccggaa acagcaggcg gtgtttctgg gtaaggatga
     4501 gcagggccag ccggtgctgg tccctcgtga tacctggcgc aagaccaata ttcagatcct
     4561 cggcctgccc gggagcggca agagcgcgat ggccaccaac gcgctcattc gctgcgtacg
     4621 cgatttcggg gacgctgtgg tgtacttcga ccccaaaggg gacgcgtggg cgccgcacgt
     4681 cttccgggcg cactgcccgg acttcaccct gctggatctg cggccgggga aaccggcgca
     4741 gctgaatctg ttccgcgatc tcgaccagta cgccctgaag aatctgctgg ttgccgggtt
     4801 taacctgagt gagaccggcg acgtggcgga ccactaccgc atcagcgagc agaaagcggc
     4861 gaagctgatt gcggagcagt tcccgcaggg ggcaaacatt cagcaggtgc tggcggcggc
     4921 gtacgcgttg ccggaggccc tgaaaaagga cgtgaagggg ctgatcacca agctggagaa
     4981 cgtggccgac ctgagcgtcc tgcagacgga cagcggtatc gatgtggcgg ggataatcaa
     5041 cggcggcggc tgcctgtacg tcatcggctc gatggacgat gaggcggtga tccgggtgca
     5101 gaagatgctg tttgcccgct gcgcgcaaat catcattgcg cgggatgagt tccggaggtg
     5161 gccgcacgcg agcatcatgc tcgatgagat taaatatctg ctgtcgaagt acgtgctgaa
     5221 cgcgctgggg acgctgcgca gccgggactg caacctgctg ctcgcgcacc agtcgctggg
     5281 cgacttcggt cagtgcgggc aggatctgcc cgctgatttt gtgaaaacca cggtactgga
     5341 taacacgccg atccgctggt tctatcgcgc ggccagccag gagtccgcgc agtgggcggc
     5401 gggccagacc ggggagatcc gggtggacgt ggagcgccgc cgggccagcc gggaggcggg
     5461 gaacgtggag cacatcagcg gcgacagctt catccagaag gaggcgcggc cgctgtttga
     5521 cgtgaacacc ctgcaacacc tgcctgacgg gttcgcggtg atgaccgggc tgggcgtggc
     5581 gcggctggcg ttcagcagcc cgctgcgggt tgagcgccgg gaaatttcgc tgaaaacctt
     5641 cccggtgctg gcgaaaacgg acccgctggc ggagtacctg cctccggccg acggcggcgg
     5701 accaaaagcg gatgatgatt ttgcggggct ttactgatga cactggagca gtacaacgtc
     5761 agccacgcga agcggcaggc gcggaacgcg gagaaaacac ggctgaccct gcgctggctg
     5821 cgggaagagc tgtgttccac ggcggagctg gttgcgcgac ggctggggat tgcggcggta
     5881 cagccggttt accgcttcct ggacagtctg gtggcgaaag ggctgctggt ccgggcgaaa
     5941 tacccggttg acggccgtca ggtcagcgtg tgggggctga cgccgcacgg ggtggcgttc
     6001 tcgttcgacg aggacgagcc gctgacggac gttattccgt ttcagccgtc aagggtgtcg
     6061 gcggcgcagc tgccgcaccg gctggccgtg cagtccctgc gcctctccat ggagggccgg
     6121 ggggccaccg gctggcggta cctgcaccgt atggccctga aagggatgaa agtgccggac
     6181 gcgctggcgg agctggacgg cagaacggtg gcatttgagg tagagcgcac cgtcaagagc
     6241 cgcaggcggt atcaggaagt ggtgtccggg tatctgttca accgccgggc gaacggcatc
     6301 gatgaaatct ggtacatctg ccctgaccgg ccgacgcttg tccgggtaca gcgggcgatt
     6361 ctggcggtgg atgaaatcgt gaatccacag accggcgagg cccgcaaaac ggtggagctg
     6421 gaccgggaga ggctgttcgc gtgctttaaa tttatgacaa cggagtgagg gattatggcg
     6481 ctgtacatgg tatttattga ggacgtggaa gacaaagacg gcgagcgctg cattatgcca
     6541 accagcgagc atctggaaga catggtggtg ctcgcctggc agaccggggc gtatgttttc
     6601 tgttttgtcg accgggccca gcatctggcg atgatgaaat ccatcgggat gtatgacgag
     6661 cggctcaaca gcgacgaaaa agccacgtac tttttcaccg gtgaaaatcc gcaatgggaa
     6721 cgggacaaac ccacaggaat gaagctgtgt gattacctct atcgaaaggt ggagcggttc
     6781 gccatcgaga acggtgtgca ggtggttgat gccgggaagt gctcaccgga gcagattaac
     6841 gcgatgattg aggtggtcag cgagcggcac ggggaaagcg cggtgaacga gaagctgaaa
     6901 gcggagctga tgcgggaata taacagccgc tattttaacg tgccggtgcc tgaactgccg
     6961 gaggacaaac ccgcccgccg gtggtggccg ttcggcaaaa aataacgcca gcaccggcat
     7021 ggccggtgct gttattcttc cccgagctta tcccgcatca cctgccagag tgtttccgca
     7081 tcctcgtgaa gccgttcgca catgtcggcc ggggtatcca tgtccagctc gttcagcttt
     7141 tccagcaaca gcagcgactg atccttaatg aatttagcca tattgagggc agttttttcc
     7201 tgcttcgtca tgttctccat tcccttcggt aagttctcag ctaaaccagc cggaccagaa
     7261 gcgttttttc ttctgtgtca gttctgttgt caatctgtcc acttcggcct ggagtttccg
     7321 gcgctcatca tcgtgctggc ggcgttcttc ttcccgggat gttttatctt ccagcatcag
     7381 ggtgacagcc tgccggagat cggccagttc cttttgcatt gccagaagct gttcctgtgg
     7441 gaaatttttt tgtgtatttt cacggtgtga aatgccggga atttgtttct cagggatatt
     7501 cacatttccg tatacacgga tcagttctga cacatccacg acagggttgt ttttcccgtc
     7561 acgtgacacg gtaacctttc cctgttttac atgattgtat agcgttcttc tggtaatgcc
     7621 cgcggcttgt gctgcctgtg aaaggttgag caatgttttc gccactgtat acccctgatt
     7681 ttatcgggtg tgaaaggtgt gtaatttctc acggtaaatg tatcgctgaa ttttacccgt
     7741 ttttccgttc aggatgtggt tttctgacgc gctgcgcttg tcaaaccggc gacggccacg
     7801 caatgaattg cgcccccgtc atcggttatt agaggctgtc tgcccccgga tacccggatt
     7861 ctttctgatg cctgacggtg acgatcacaa tctggtccat ttcccggtcg tggcggtaga
     7921 gcatcacata gccactgtcg ccaaacccga tcaccagttc cttatattcc agcggcagaa
     7981 acggcaccgg gcggccaatg tctggcaatg ttttcagttg ctggatagcc cggacgatca
     8041 cctcacctcc ttttctggct gccagccgat ttttggtttt gagaaagtcc tgaaggcgtt
     8101 gtaaatcgcg ctgtgccagc gcggaaatcg ttacctgtgg catggcggag catcctgctc
     8161 gttctcagtt ccccaggtac ttatccaggc ttccgcttct tccgcagtaa ggtgcagacc
     8221 cgtttcctga tagtgctgcc atgcggcctg tccgtcccgc aggtactggc tgcgcttctc
     8281 ttcccggtcg atgtattcgg tgattgcctc cagcattagc gcgtgcgctg agcggtgccg
     8341 gtcatcggcc agcgttttca gccggtcctt aagttcttca tcgagcctga tggatgttgc
     8401 tgatgacata aaagcccctt tgtagtcata tgtattacac atgactactt taatccgtgg
     8461 cggcgctatt tgccagaaat acggcttttg ctgcccattt ttcagcttcg cttccactaa
     8521 tcagggctgc tacaggctca ctcatcatcc aggactattg cagcctgcgg gttaaaggac
     8581 ctgttactgg cggcaaccat catcattgag cttttgggtg gtgactggtc gcggatcctg
     8641 taagccgtca tttttcagac tgaattcaaa atttataacc tgtcgcattg ccgggtgccc
     8701 tgcgggatat agccagtgcc tcattgccgt cagcgtttcc cgaaaaaaaa ggtgacagcc
     8761 ggtaagcgac acgttgtcca ctcgtccatc gtccagaata tcaaacgtgg cggtcacctg
     8821 accttcgatg cgttttgctg cggcctgtac cgggtaggtt ggctgcagtg tggttacggg
     8881 ttttagtggt atcgcatgta ccggagtgag gaacatggcc gccagcactg tcagagtaaa
     8941 agctttcatg gtctgaaatc cagaggggcg tttttgaata tttgccaggt attttcatcc
     9001 ggtgccggcg gaattttggc gtgggtaacg gctgatattg ctgctttaca gagtttcgga
     9061 tctcctcctt cagcaatggc gctgttcaga gtgccatcac gttgtatgga tatccgcacg
     9121 gtgcacattt ttcccgcata ggcctctggt tcaaacatct gcatttcaac agcggtcttg
     9181 atggccgcag cgtagctact gattttagat gacttatcca gagtataaat tttcggcggc
     9241 tgggtggtgg aatgcttaca ggccgtcagg agtaaggggc tgcacaatgc cagcaggata
     9301 aagggtatta aacgcatcgg gttgatgacc tgtcagtaaa aaatgttaca tgtatatttg
     9361 tcactcttta cggccacgtt catttacata aacacggctt atcgtatcgt ttttacacca
     9421 aacggcccgc catgcagttg cgtgcatagg catgcagcgg gttacaattt accgggcgcg
     9481 tttccggcgg ttttccgggt ggtttgttgc ctgttttatc ccgtagccgc cggaaacgcc
     9541 ctcagaccgt ctgagcggtg cgggcaatgc gacgttatgg taaatacccc ttaccggcgg
     9601 cgctgtggct gccaggtgaa ggtggtttcc gtagcctcgt atttctcccg acgcatttcc
     9661 tgcgcccgcc tggcttcacg ttcgtcctga atggcacgaa gcaccctttc ccgcacctgt
     9721 tgttttaccg cctttggcag cgttttgttc cctttcaggc ccatggccag tgcctgagcg
     9781 acaaaggcgt ccgccaggcg cttttcttca gcgggagtca gtttcaggtg cgtcatagtg
     9841 gcctccgggc gtctcaggtt tcccgaacag tttaaccgca ggtcctctcc tcatgccagt
     9901 tagctttcag atgtgtctat accatctgaa atgtaagtgt gatgaatgtc ttaaataata
     9961 aaatttgttg ccatgagaaa ttttcgggaa aagacgcgat ctgcttacag gtggctccgg
    10021 gcgctttttt gtgcctgaag atgagaacta atctcatata ttgacacctg aaaacgggag
    10081 gagtaaggta ataattatac tgtgtatatg tacagtgtgc gccgggagac cggttagaga
    10141 tcaaggggtg aaagtccccg accattgaag gaccagcaat ccacaaggtc cccgagtcat
    10201 gcgttgcata ccgcgaggta tggggcgaag cgttgacagg ggtgttgaca ggccagccat
    10261 tgagccacga aatgtatatt aaattaccgg gtgccgacgt tgtactgtta acggaaggca
    10321 acatcatagg gtgcgatact gcgagtgcca catggacccg gcggggtctg agaccctggc
    10381 atgtcaatac gatctctacg cgggaaccgg gagatctccc ctctgaccat ctgccagtgt
    10441 cggagatggc ccgcaccggg aagacgagga gtcatagccg gtgatgtacg gagaggagaa
    10501 gtcggactcg ctcatagtag cggcgaagca ggcgaacaac ccgaaaggag cggagtcagt
    10561 ggagcgaagg agcggggcca aggggaacgc ggaacagcca cacatgcgcc ggacacagag
    10621 ccgggaaagc atgtcacaga ggctgtcacg cgtgcgggaa gctgcgaagc agcggaagaa
    10681 agaacggttt acagcattgt tccacctgct gacagtcgaa gcactggaag ccgcattcct
    10741 ctccctgagc aggaaagcgg ccgccggagt ggatggcatc aggtggatgg actacgccgg
    10801 aaacatgaag aacaacataa cagatctgca ccggaggcta catcagggca gctacagggc
    10861 gcagcccggc aggcgtcact acatcccaaa agcggatgga aaacaacgcc cgctcggcat
    10921 cgcctcgctg gaggacaaga tcgtccagta tgcgctggtg aaaatcctga acgcagtcta
    10981 tgaaaacgac tttatggggt tctcatacgg gttcagaccc gggcgaagcc agcacgatgc
    11041 actggacgca ctggccacag ggctggtacg cactaacgta aactgggtac tggatgccga
    11101 catcagtcag ttcttcgaca gggtgagcca cgaatggctg atcaggttca cagagcatcg
    11161 gatcggcgac cggagggtaa tcaggctcat acgtaagtgg ctcacagccg ggacgtcgga
    11221 ggagggtcaa tggcgagcaa cggaggaagg caccccacag ggtgcggtca tctcaccgct
    11281 gctggcaaac atatacctcc actacgtctt cgatctgtgg gcgcatcagt ggcgacgtcg
    11341 ctatgccaca ggcaatgtgg taatggtcag atacgccgat gacatcgtca tcgggttcga
    11401 caaacgatac gatgcccggc gcttccgtat agccatgcag cgcagactga gggagttcgg
    11461 actcacggtt cacccggaga aaacccgtct gatggagttc ggccgcttcg ctgccgaaaa
    11521 ccgtgccatc aggggaaaag gcaaaccaga aacgttcaac ttcctcgggt tcacgcacat
    11581 cagcgggaaa gatcgcaacg gcaggttcat gctgatacga aagacccgcc gggatcggat
    11641 gacggcaact ctgaaagcca tcaaagacgg tctgcgaagg cgctggcatt actcaatccc
    11701 cgaacaggga aaatggctca ggagagtggt tcagggatac ctgaactatc actcggtacc
    11761 gggcaacttc cccaccatgc agaagttcag gacacacgta acaaacctct ggcgccgggc
    11821 gctcaggcgc aggagccaga aggatgatac gacctggacg aaagcaaaca aactggcagc
    11881 cgcatggcta ccaagggttc gggttcttca tccatggcct gtggagcggt tcaccgccag
    11941 acacccgagg caggagcccg gtgcgtaaat
//
