LOCUS       CP097474               21755 bp    DNA     circular BCT 03-AUG-2023
DEFINITION  Klebsiella pneumoniae strain 16HN-12 plasmid p16HN-12_5, complete
            sequence.
ACCESSION   CP097474
VERSION     CP097474.1
DBLINK      BioProject: PRJNA503173
            BioSample: SAMN10353622
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 21755)
  AUTHORS   Yang,X.
  TITLE     A multi-drug resistant plasmid mediates transmission of virulence
            plasmid in Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21755)
  AUTHORS   Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2018) The Department of Applied Biology and
            Chemical Technology, The Hong Kong Polytechnic University, Hung Hom,
            Kowloon, Hong Kong 999999, Hong Kong
REFERENCE   3  (bases 1 to 21755)
  AUTHORS   Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) Department of Infectious Diseases and Public
            Health, City University of Hong Kong, Kowloon Tong, Hong Kong
            999999, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina NextSeq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/19/2022 10:53:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,972
            CDSs (total)                      :: 5,855
            Genes (coding)                    :: 5,678
            CDSs (with protein)               :: 5,678
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 64 of 177
            Pseudo Genes (incomplete)         :: 122 of 177
            Pseudo Genes (internal stop)      :: 25 of 177
            Pseudo Genes (multiple problems)  :: 32 of 177
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..21755
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="16HN-12"
                     /isolation_source="pus"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p16HN-12_5"
                     /geo_loc_name="China"
                     /collection_date="2016"
     gene            1..978
                     /locus_tag="ELM62_29010"
     CDS             1..978
                     /locus_tag="ELM62_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003887 - DNA-directed DNA polymerase activity [Evidence
                     IEA]; GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WKV69891.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNTL
                     SRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVELP
                     FVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSAYYD
                     TERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPAPISL
                     ARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPRLKAPN
                     DESKENQLPPSPAEKISPEMAEKLALLEKLGITLDDLEKLFKSR"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(1221..1961)
                     /locus_tag="ELM62_29015"
                     /pseudo=""
     CDS             complement(1221..1961)
                     /locus_tag="ELM62_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /name="site-specific integrase"
     gene            2309..2582
                     /locus_tag="ELM62_29020"
                     /pseudo=""
     CDS             2309..2582
                     /locus_tag="ELM62_29020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611340.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_process: GO:0015074 - DNA integration
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(2719..3255)
                     /locus_tag="ELM62_29025"
     CDS             complement(2719..3255)
                     /locus_tag="ELM62_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004117808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer protein"
                     /protein_id="WKV69892.1"
                     /translation="MENINNDVPQHQPYRNEKVFNSGKTALEFNFSETNGSVNLILAGP
                     LVSKPGSFDWTGQKAFSTKLSDDEVITLCMAFLRLTHEAVLKDKKTKHHNKQVYKNVKV
                     TFDGKSTAMMEGGVVAINKDERDINFIHKISIDPAACLRLGLFLLSVILARNPGVPSDA
                     VLTCMRLNANAQLQK"
                     /name="plasmid transfer protein"
     gene            complement(3466..4534)
                     /locus_tag="ELM62_29030"
     CDS             complement(join(3466..4312,4311..4534))
                     /locus_tag="ELM62_29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611505.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_function: GO:0004803 - transposase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WKV69899.1"
                     /translation="MAFALKQAETGTRVEEVCRKMGISEATFYNWKKKFGGMGVTELRR
                     LRQLEEENQCLKRLVADLSLDKEMLQEVIKKKVLRPAQKRQAVHFLREAYRISVRRGCG
                     LLMQSRTVYHWQSRRDDRAITLRIREIAEPRIRYGCPRIHIQLRREGWPVNHKKTHRIY
                     CLEGLNLRKKRPRRHVSAAHRQQRPVLTGVDQCWSMDFVSDNLFNGRRFRALTVVDNFS
                     RECVAIHAGKSLKGEDVVGVMERLRVPGKRLPVRIQTDNGSEFISKSLDKWAYEHGVTM
                     DFSRPGKPTDNPFIESFNGSLRDECLNIYWFLSLEDAQDKLDNWRREYNHERTHSSLND
                     MTPAELIRSLRKDEAL"
                     /name="IS3 family transposase"
     gene            4987..6009
                     /gene="istA"
                     /locus_tag="ELM62_29035"
                     /pseudo=""
     CDS             4987..6009
                     /gene="istA"
                     /locus_tag="ELM62_29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /name="istA"
     gene            6006..6788
                     /gene="istB"
                     /locus_tag="ELM62_29040"
     CDS             6006..6788
                     /gene="istB"
                     /locus_tag="ELM62_29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSen3 family helper ATPase
                     IstB"
                     /protein_id="WKV69893.1"
                     /translation="MMVELQHQRLMVLAEQLQLDSLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGVPQKQIQSLRSLSFIERNE
                     NIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTVQRQGRYKTTLHRGV
                     MAPKLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDTALT
                     SAMLDRILHHSHVVQIKGESYRLKQKRKAGVIAEANPE"
                     /name="istB"
     gene            complement(6854..7503)
                     /locus_tag="ELM62_29045"
                     /pseudo=""
     CDS             complement(6854..7503)
                     /locus_tag="ELM62_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_function: GO:0003677 - DNA binding
                     [Evidence IEA]; GO_function: GO:0004803 - transposase
                     activity [Evidence IEA]; GO_process: GO:0006313 -
                     transposition, DNA-mediated [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(7572..8123)
                     /locus_tag="ELM62_29050"
     CDS             complement(7572..8123)
                     /locus_tag="ELM62_29050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="WKV69894.1"
                     /translation="MLEVSLSSMALRPEKMRPPVGSYVAVAEFTLLRTVRLLDLSSLKG
                     LAVNGFSRFDPEYLLRYERSSFIQNLSRKLVMPVVPELEETNYLLTQAIADYLSTFEHC
                     ELDGILFASAQKPKESTDPSVMNIILFHKSSGVLNADRRFREAEVNMYQHDEDISYFDP
                     EIRTTPDDFRREFKVLSRKK"
                     /name="RES domain-containing protein"
     gene            complement(8142..8642)
                     /locus_tag="ELM62_29055"
     CDS             complement(8142..8642)
                     /locus_tag="ELM62_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKV69895.1"
                     /translation="MSMETLAEKVDWLIENYYRGGEYNHYLEEYEGEPLFSVLEQEVTA
                     NDNIIDDLSELLEELWFDWSSHDRKYGDEPHFVEAVTISGNLSRKWNSMEGNLERVIDS
                     LIWTRWQLSKRFLLTFTKIIPQHSSILSLTNQFSVVVFFSQRMHLKSHYVCPSRVSDLL
                     LLS"
                     /name="hypothetical protein"
     gene            complement(8815..9138)
                     /locus_tag="ELM62_29060"
     CDS             complement(8815..9138)
                     /locus_tag="ELM62_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKV69896.1"
                     /translation="MTFQELDACIAVSGRRSIASALIAFILDALDDGQDGVDLDIFQSH
                     TRFVRNNVTTVASYLQLHGIIHILYYRDGAAERQYESVNNYGRWAKQHYRPSEALIQLH
                     RRD"
                     /name="hypothetical protein"
     gene            complement(9135..9572)
                     /locus_tag="ELM62_29065"
     CDS             complement(9135..9572)
                     /locus_tag="ELM62_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKV69900.1"
                     /translation="MKSTPFTEMATAFRGQIVRLWALRYPGTQSEAAAALTEAAINLGY
                     VTRNRPIPGAALLSWASNPTETPLWAAQTALTLMLSIGWKPESNQDWCGMSALIFRANR
                     TLPLEQLVASLPDSIDRQTATGWFVAAIEEDSSYRYNRKSR"
                     /name="hypothetical protein"
     gene            complement(9572..10603)
                     /locus_tag="ELM62_29070"
     CDS             complement(9572..10603)
                     /locus_tag="ELM62_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0000910 - cytokinesis [Evidence IEA]; GO_process:
                     GO:0007059 - chromosome segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="WKV69897.1"
                     /translation="MAKKPFMKRLQAQQVMKKNAAVVSDIEQVIANKQPDEESHDIAAH
                     KIMADIARPDIEPDGQIIRVDINLVYAEEQVRPEEDFEEDIIDGMSDTFETIGMLTPPR
                     CYPRDRRGYQIWLGETRVRTARKRGDTHIDIYVGKPPRERKERIIGQLIENLQQSGLKP
                     LATAKSFYELKHEFNMTGEQIAKTMGKPTSFVSKHLRLLDAPENVTALLRDKVTSDIDL
                     AYTLIQVNEKSPEEAEKLVAAARESGISRSQVKAVLDAVKNKSKGEISHAKSKAQNKKP
                     TKPLDSNSDVKTWQVVVEIDGQQGVIMTDRAPDEAGYVWVKLEIGEISVEASELRVKGM
                     RSL"
                     /name="ParB/RepB/Spo0J family partition protein"
     gene            complement(10603..11469)
                     /locus_tag="ELM62_29075"
     CDS             complement(10603..11469)
                     /locus_tag="ELM62_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WKV69898.1"
                     /translation="MTLKIGPIGKLIVVCSQKGGIVKSQSSSEIAHDLRKAGFRVLEID
                     MDWTTGLTERAFPDDLPYEIEREPLSNSFTPGEANTYQLFFPETEVRPIELPGGRFFIG
                     ATSELNEINYRNSDCMFDFRDRIEELKKQFDFIIADSAPSYSNVMIAAHMSADYLLIPT
                     LLEKQARNAVGKQLTYMQKIKRNYNPSLQFMGTYVSQTQVKNYKKPLKEGYLGPIDTLN
                     LNLLIEILQGKGYSEEYILAYISFVPAAAKEAIELGLSFDEYDPKCLPAIQYKELTQKI
                     IMLAGGK"
                     /name="AAA family ATPase"
     gene            complement(11993..12232)
                     /locus_tag="ELM62_29080"
     CDS             complement(11993..12232)
                     /locus_tag="ELM62_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="WKV69886.1"
                     /translation="MKKVICSLCHGRGGDVIITCSNCNGSGYDPQDDNPFAQCHTCYGE
                     GEENADVCPRCGGDGYYYVDEDEEEDEDEDEEGL"
                     /name="molecular chaperone DnaJ"
     gene            12421..14169
                     /locus_tag="ELM62_29085"
     CDS             12421..14169
                     /locus_tag="ELM62_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014599331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKV69887.1"
                     /translation="MKEIVLKLSEAENVLREWFEAGIAFNLIFGPLHFRKESGLVHLRK
                     CLAKIPLALRPQYYDILEKAFSPRHNILDILFRNNYDYDSLMLRGQLYAYAECLTKNYP
                     KMPLKLLLTAAATPHSVLEPKKIIHAYYKVRTELERNSRQKLNITIVDPTLIALCKLVS
                     ERQLTSNLVDIEYGNPQGKMTPFRIHSFDLFTNKYRRLSNEKFSLDQVHGHFISIAHKL
                     ALGRDPLNEVSHPLLKDKKYTQWAPILHALCRKHENSSQVEYYKKYSKKFPLKYKHEFD
                     SNSINHQIEKLNKRYCSLFRFLKPSPENFSQNQRNALKTTPPEVMQKMIVYHMIMFYFS
                     LIKNAAWYIKVRDFMISLKMSYPQDYASKLFAFSSGDECMDDTLYNSFNEIFSANPVGL
                     FPWMFSGLLPEPMELMTHYFSNKKNKDIEHIDKKNKSFRNIDLAASVLIIPKFLNNLDR
                     AKGINPSIMVKLPSNNSESCIFYTATGIPKEEGLYLAELFSKGLYIQRNIEESLTMELR
                     EIEDLLLGICLLWHESFVGKISLSKFVNILQQNEINDISERTLKARKDKAKYWLMQWPS
                     QLPLIS"
                     /name="hypothetical protein"
     gene            complement(14148..14315)
                     /locus_tag="ELM62_29090"
                     /pseudo=""
     CDS             complement(14148..14315)
                     /locus_tag="ELM62_29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            14619..15548
                     /locus_tag="ELM62_29095"
     CDS             14619..15548
                     /locus_tag="ELM62_29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="WKV69888.1"
                     /translation="MKKKTTTPTPHDATFRQFLTQPEIARDFMELHLPAELRAICDLTT
                     LKLESGSFVEDDLRQYFSDVLYSLKTAGGDGYVHVLIEHQSSPDKHMAFRLIRYAVAAM
                     QRHLEAGHKKLPLVIPVLFYTGKRSPYPYSTRWLDEFDDPTLAGSLYSNAFPLVDVTVI
                     PDDEIADHRSMAALTLLQKHIHQRDLAELVDRLAPILLAGYLSSSQVISLVHYIVQAGE
                     TADAEAFVRELALRVPQHGDALMTIAQQLEQKGIEKGIQLGEQRGIEKGRNEGKLEVAR
                     TMLSSGIDRSTVMKMTGLTEEDLAQIRH"
                     /name="Rpn family recombination-promoting nuclease/putative
                     transposase"
     gene            complement(15693..16472)
                     /locus_tag="ELM62_29100"
     CDS             complement(15693..16472)
                     /locus_tag="ELM62_29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WKV69889.1"
                     /translation="MTGLVTLGPQPGTAAQLPVAIDYPAALALRQMALVQDELPKYLLA
                     PEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQRAE
                     KAARTAGRAPAGNQVHRLVPLSDHQYVSQLQMMVATLKIPLERRNKRTGRTEKARIWQI
                     TDRTVRTWFAEAVEAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMGHKSI
                     SSTEVYTKVFALDVAARHRVQFQMPGTEAVAMLKERI"
                     /name="site-specific integrase"
     gene            complement(16469..17290)
                     /locus_tag="ELM62_29105"
     CDS             complement(16469..17290)
                     /locus_tag="ELM62_29105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKV69901.1"
                     /translation="MEIMKDMMIDIEAERFNEWLEENYPDVVPESEEWEQAANLYYWEQ
                     EALADQAQWDHEHGLFVVSLNDVHQRYRHARQELKKLYALLDRELPEIVYRMSFVHAVT
                     VMEAYLMYCAKALLEHDWPLERYFEAYYLPFARASKKEKLATSKMPLSRFRPVARNVVA
                     RMTFHNVETIERYFSAVLHIPPIWPVRPLGIIADWRNDLVHRNGVDEHDVPRVISAQQL
                     QNALQRVSDLIEAAHQSLSQEVDYFGNWRSEENREIIASALNISTDSDVSR"
                     /name="hypothetical protein"
     gene            19940..20581
                     /locus_tag="ELM62_29110"
     CDS             19940..20581
                     /locus_tag="ELM62_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WKV69890.1"
                     /translation="MNADTWTRLQAFRHALELTSCEASLTAGYDHLKDFPAGCSELASQ
                     TLTDYLTEDGSNLYSCIVGMQWDNGPGRYGHVIADPARDYIDLTLDQFPGYHNRIVAEP
                     VESGGQLAADLNREPAISTADGIVASPSDKVNYITEQKNRQPMVIITECYKLIRMTARF
                     IPMCQSSQLFQHQVFPRLFIISDVGSCSLFRNTLDRLATDWKVTLSILSL"
                     /name="hypothetical protein"
     Typing          2..978
                     /name="IncFIB"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.751"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtggataagt cgtccggtga gctggtgaca ctgacaccaa acaataacaa caccgtacaa
       61 cctgtggcgc tgatgcgtct gggcgtcttt gtacctaccc ttaagtcact gaagaacagt
      121 aaaaaaaata cactgtcacg caccgatgcc acggaagaac tgacgcgtct ttctctggcc
      181 cgtgcagaag gattcgataa ggttgagatc accggccccc ggctggatat ggataacgat
      241 ttcaagacct gggtggggat cattcattcc tttgcccgcc ataacgtgat tggtgacaaa
      301 gttgaactgc cttttgtcga gtttgcaaaa ctgtgtggta taccctcaag ccagtcatca
      361 cgcaggctgc gtgagcgcat cagcccttcc ctgaaacgca ttgccggtac cgtgatctcc
      421 ttttcccgca ccgatgagaa gcacacccgg gaatacatca cccatctggt acagtcagcc
      481 tactacgata ctgaacggga tattgttcag ttacaggctg atccccgcct ttttgaactg
      541 taccagtttg acagaaaggt ccttcttcag cttaaggcga ttaatgccct gaagcgacgg
      601 gagtccgccc aggcacttta cacctttata gagagcctgc cccgggatcc ggcaccgata
      661 tcgctggcgc ggctgcgtgc acgcctcaat ctgaagtctc ctgtattttc ccagaaccag
      721 acggtcagac gggcaatgga gcagttgcgc gagattggat atcttgatta cacggagatc
      781 cagcgggggc ggacaaaatt cttctgtatt cactaccggc gtccccggtt aaaagcgccg
      841 aatgatgaga gtaaggaaaa tcagttgcca ccttcacctg cggaaaaaat cagtccggag
      901 atggcggaga agcttgccct gcttgaaaaa ctgggcatca cgctggatga cctggaaaaa
      961 ctcttcaaat cccgctgaac ataaactgta gtcagataag agagactctt tactgactac
     1021 agtttatatt aacaggtgca gtgagtgatg tgctcactgt agtttatatt cagtttcctg
     1081 ctgcactgtc tgtggctgag ctgccatctg cctgttcctt acataaaaca cggcttactc
     1141 actacagttt atctacctgc tatagcttat gttatgcctg ttctgcttac ctccgcttat
     1201 ataaggaaga gcgtatgctg tcacttcagg ggaggcagtg tacgcaggat ctctgcagca
     1261 tcgcgcccgt cacctgtgaa aggcactgcc agcgtggcag ccatatccag cgcaaaaact
     1321 ctggtataaa cctccatcga acgtggatcc ctgtgaccag ccagtgcctg gatgactttc
     1381 cggggctggc ggtgatagag catgtgcatg atatagctgt gccggaaggt atgtggtgtg
     1441 accggaatcg aaaaatgcac gccatcagct tcagcccgct tcaccgcctg tttcagccag
     1501 ttgcgcattg tctcatccgt gaccggccat aacggctccc gccggcgggg cgggtggtga
     1561 ccatccagct ttccatctga cgcacaaaac tgccatccgt cagcggcacc aggcgcactt
     1621 catcttttgg cggccgcccg cggcgcgccc gcactttctc cgaaagcacc ctaacaaaag
     1681 ggcgcagacc atccaggtcg aaggattccg gggtgagcgt ccgggcttca ccgatacggc
     1741 tcccggtatt ccacatggtg gcgaacaaca tgtggtgacg ctggtccggc atataccaga
     1801 ggagggcgct cacttccggc gccaggaggt aagccggcgt ggcacctgtt gaggtggcca
     1861 tccgccggag tgccaccgcg gtcgcaaaat caacgcccgg ggcggatcgg cagcagggag
     1921 acgcgatctg gtgaattctg caggggaatg atgctgttca tgagttattt ttcacgtagc
     1981 gcacaacggg tttggataca gctattctgg cctgaagcgg ctgatcctgc catgacaata
     2041 aatcgcagcg cgcggatcct tttgttgtct tatcccacat tattgcgggt aaaaccaaat
     2101 gcgtaaagga tccttttgcg gttaagatct atgcttaagc cagttatgac gcggcctgca
     2161 ggcactttcg tcgtttttgg gagaaatggg gtaatgctag gacaaacagc tatgttgaca
     2221 ctggcagtaa tggagacgag aaacggctac cttccagatg cgatcggcag gaatgatctt
     2281 caacatgttc cgtctgtggt tgtgtaaggg tagtcagtac ggcagtgacg actggcaccg
     2341 cttctgcggg ccaataacct ggcaccgagc ttgagccggc gtggcaactg ccaggataat
     2401 gcggtggccg aattgttctt cagttcactg aaaaaagaac gcattagtaa gagaacatac
     2461 caaaccggga tctggcccgg gcggatgtct tcgattacat tgaagtgttc tacaatcgga
     2521 cccggcgcca cagtcatccc ggcggcgtca gtccggaggc cttcgaacag gcctcgtcgt
     2581 gaggacagaa tttgtctaca ggcatggggt cagtccatag ttacatctaa tgtatcctat
     2641 gaaccgagcg tattttcacc attaagggaa gtatcttttt tgaaattccc gtaaaacggg
     2701 aatgataaga tagaaaagtt atttttgtag ttgagcattt gcattaagac gcatgcacgt
     2761 tagaaccgcg tcagatggga cccctggatt tcgggccagt atgacggaca agaggaaaag
     2821 acctaagcga aggcaagcgg caggatcaat actaatttta tgtataaaat taatgtctct
     2881 ctcgtctttg ttgatagcta caactccccc ctccatcatt gctgtggatt taccatcaaa
     2941 agtaactttt acgtttttgt agacttgctt attatggtgc tttgtttttt tatctttcag
     3001 aacagcctca tgagtaaggc gaagaaacgc catacacaag gtgatcactt catcatcact
     3061 cagctttgtt gaaaaggcct tttgaccagt ccagtcaaaa cttcccggct tcgatactaa
     3121 aggccccgca aggataagat tcacactgcc atttgtctca gaaaaattga actcaagtgc
     3181 tgtcttaccg gaattaaata ccttttcatt tcggtaaggt tgatgctgtg gtacatcgtt
     3241 atttatattt tccaccaaat atgtccttca ccttcgttga ttaaattctt caccagacca
     3301 atgtaggtat accctctagg ttgcttcatc gcaaaaatat aagcactggt ctccagcatt
     3361 agatagtctc ctgagggagc accaggagga acctctgtag agctaaggct tttagtgtgc
     3421 tcctttttaa ctttgaccct ggcccaaatt cagtgcagtg ctaaatcaga gagcttcgtc
     3481 tttccggaga cttcggatta attcagccgg agtcatgtca tttaatgatg aatgcgttct
     3541 ctcatgatta tattccctgc gccagttgtc gagtttgtcc tgcgcatctt ccagtgagag
     3601 aaaccagtaa atgttcaggc attcatcccg cagactgccg ttaaatgatt caataaacgg
     3661 gttatccgtt ggttttccgg ggcgggagaa gtccattgtg acaccgtgct catacgccca
     3721 tttatccaga cttttcgaga taaactcgct gccgttatct gtctgaatac gtaccggcag
     3781 gcgcttgccc ggcacccgca acctttccat cacaccgacc acatcctcac ctttcagcga
     3841 ctttccggca tggatcgcca cacactcccg actaaaatta tccactacag tcagcgcccg
     3901 gaaacgtcgt ccgttaaaca ggttatccga cacgaaatcc atactccagc actgatcgac
     3961 tccggtcagg accggacgtt gctgacgatg tgctgcactg acatgcctgc gggggcgctt
     4021 tttgcgcagg ttgaggcctt ccagacagta aatccggtgt gttttcttgt ggttaacagg
     4081 ccagccttcc cgccgcagct gaatatgaat acgcgggcaa ccgtagcgga tccggggttc
     4141 cgctatttcc cggatacgca gggttatcgc ccgatcatcc cgccggctct gccagtggta
     4201 aacggttctg ctctgcatca gcagtccgca tccccgccgg acactgatac ggtaagcctc
     4261 ccgcagaaaa tgtaccgcct gacgcttctg agccggcctc agaacttttt ttgataactt
     4321 cctgcagcat ctccttgtcc agacttaaat cagccaccag acgtttcaga cactgattct
     4381 cttcctcaag ctggcgcagg cggcgcagct ctgtcacgcc catgccgcca aactttttct
     4441 tccagttgta aaatgtggct tcagaaatgc ccatctttcg gcagacttcc tccacccgag
     4501 tgccggtttc ggcctgtttc agcgcaaatg ctatctgttc ttaggtatag cgggtctttt
     4561 tcatggcgat gtgcctctcc gtgagatgga agaaagaccg gagacttcag tttagcctag
     4621 cactgttttc agggaggaga tcagatcttt ggtaaaggtg gctgttccaa tgttctttac
     4681 cctacctgtg accattaatc ggcaagttct gaagtgatta gtagccctga attcgttgtc
     4741 ctggaaacgc ctgtaaacct cttcaggagt atgtatccga ttaacaacgt tgaccatttc
     4801 cggttctgct ggttttagag gtacttgagt taacatctgg agtttctgtg tcatggttcg
     4861 gaggtgatgt aaggacacca tgaacaacaa gcactgcaaa tgttaccaac ggtgcgaaag
     4921 tgatccatat ccgacgcctg aaattagatc caggggttaa tctgctctcc tgattcggga
     4981 gagcttatgg tcacttttga gacagttatg gaaattaaaa tcctgcacaa gcagggtatg
     5041 agcagtcggg cgatagccag ggaactgggt atctcccgta acaccgtaaa gcgttatctg
     5101 cttgcgcagt ctgaaccgcc aaaatatacg ccgcgttccg ccgtcgcttc actcctggat
     5161 gaacaccggg actatattcg gcaacgcatc gccgatgccc atccttataa aatccctgca
     5221 acggtcatcg cccgcgagat cacggagcag ggttatcgtg gtggcatgac catcctgaga
     5281 gagttcatac gttcactcgc catccctcag gaacaggagc ccgtcgtgcg cttcgaaaca
     5341 gaaccaggac gacagatgca ggttgactga ggcaccatgc gtaatggcaa atcaccactt
     5401 catgtgtttg ttgccgttct ggggttcagc agaatgttgt atatcgagtt cactgacaat
     5461 atgcgctacg acaccctgga agaatgccac cgtaatgcgt tcagcttctt cggtggcgta
     5521 ccgcgtgaag tgctctacga caatatgaaa acggtagtgt cacaacgcga tgcctatcgg
     5581 accgggcaac accggttcaa cccttcactg tggcagttcg gcaaagagat gggcttctct
     5641 ccacgactgt gccgcccctt cagggcacag actaaaggca aggtggagcg gatggtgcag
     5701 tacacccgca acagcttcta catcccgcta atgacccgcc tgcgtccgct ggggatcacc
     5761 gtcgatgttg aaacagccaa ccgctacggc ctgcgctggt tgcacgatgt tgctaaccag
     5821 cgcaggcacg aaacaatcca gacccgtccc tgcgatcgct ggatcgagga acagcagtcc
     5881 atgctggcgc taccgccgga gaaaaaacag tatgacgtgc tggtagacga aagcctggtg
     5941 accttcgaca gacagccact gcatcacccg ctgtccatct atgacacgtt ctgcagaggg
     6001 gccgcatgat ggtggaactg caacatcaac gactgatggt ccttgccgaa cagctgcagc
     6061 tggacagcct tatcagcgcc gcacccgctc tgtcgcaaca ggctgtggat caggaatgga
     6121 gctatatgga cttcctggag catctgcttc acgaagagaa actggcccgc caccaacgta
     6181 aacaggcgat gtatacccgg atggcggcct tcccggcagt gaaaacgttc gaggagtatg
     6241 acttcacctt cgccaccggt gtcccacaga agcaaatcca gtcgctgcgt tcactgagct
     6301 tcatagagcg caacgaaaac atcgtgttgc tggggccatc cggcgtgggg aaaacccatc
     6361 tggctatagc gatgggctac gaagcggtac gggcgggcat caaggttcgc ttcacaacgg
     6421 cggcggatct gttgcttcag ttgtcaacgg tacagcgtca gggccgttat aaaacaacgc
     6481 ttcaccgtgg cgtgatggca ccaaagctgc tcatcattga tgaaataggc tatctgccgt
     6541 tcagtcagga agaggcaaag ctgttcttcc aggtcatcgc caaacgttat gagaagagcg
     6601 cgatgatcct gacctccaac ctgccgttcg ggcagtggga tcagacgttc gccggtgata
     6661 cggcgctgac atccgcgatg ctggaccgga tcttacacca ctcacatgtt gttcaaataa
     6721 aaggagagag ctatcgactg aaacagaaac gaaaggccgg ggttatcgcg gaagctaatc
     6781 ctgagtaaaa cggtggatca atattggacc gtcggtggag atgtaagtgg atcacttttt
     6841 acccgtcgtt gacagccagc gcgtccatga caaaatccat cgaccaggtc aggttgggcg
     6901 cctccgggcg gagcagtggc agacgttctg ttgccagccc tttacgacgt cgtctgcgtt
     6961 ttacgcccag cccgttaagg tggtaaagac ggtacacgcg cttgtgatta acatgaaggc
     7021 cttcacggcg cagtaactgc cagatgcgtc ggtagccaaa acgcctgcgc tccagtgcca
     7081 gctcagtgat gcgccctgat aaatgcgcat cagccgccgg acgctgagcc tcatagcggc
     7141 aggtcgacag ggacaaacct gtaagcctgc aggcacgacg ttgcgacaga ccggtcgcat
     7201 cacacataaa ctcaacggct tcccgcttct ggtctgtcgt cagtactttc gccccagagc
     7261 cacctgaagt gcctccttat ccagcatggc ttcggcaagt agcttcttga gtctggtgtt
     7321 ctcttcctca agcgacttca ggcgcttaac ctcgggcacc tccataccgc catacttctt
     7381 acgcctggtg taaaaggtgg cgtcggaaat ggcgtgctta cggcagagct cacgggcaga
     7441 aaccccggct tcggcctcgt ggagaatact gatgatctgt tcgtcggaaa aacgcttctt
     7501 catggggatg tcctcatgtg gcttatgaag acattactaa catcgcggtg tattaatcaa
     7561 cggggagcag gtcatttctt acgtgatagc accttaaatt cacgccggaa atcatcggga
     7621 gtcgtcctta tctctgggtc aaagtatgaa atatcctcat cgtgctgata catatttact
     7681 tcagcttccc ggaaccttcg gtctgcattc agtaccccag acgatttatg aaacaggata
     7741 atattcatga cagaagggtc tgtgctttcc tttggctttt gcgcggaagc gaacagtatc
     7801 ccatcgagtt cgcaatgctc aaatgttgag agataatcag cgatagcttg cgtcagcagg
     7861 taatttgttt cttcaagctc aggaacaacc ggcataacaa gttttctgga gagattctga
     7921 ataaaactac tgcgttcata tcgaagaaga tactcaggat caaagcggct aaatccgtta
     7981 acagccagcc ctttgagact ggagaggtcg agtaacctga ctgtccgcaa caaagtgaac
     8041 tcagcaacag caacataact accgacaggt ggccgcattt tttctggacg tagcgccata
     8101 gaagacagag atacctctag cattcattct gccggaaggt gtcagctcag gaggaggagg
     8161 tccgaaactc gactcgggca tacgtaatgc gatttcaagt gcatcctctg actgaaaaat
     8221 acgaccacgg aaaattggtt tgttaggctc aaaatcgatg aatgctgagg gatgattttc
     8281 gtgaaggtaa gtaaaaacct cttcgaaagt tgccatcgcg tccatattaa agaatcgatt
     8341 actctctcta agtttccttc cattgaattc cattttctgg acaggtttcc tgagatggtt
     8401 acagcttcaa caaaatgagg ctcatcacca tattttctat catgtgagct ccagtcaaac
     8461 cataactcct cgagcagttc cgataagtca tcgatgatat tatcattggc tgtgacctct
     8521 tgctccagaa cagagaagag aggttcacct tcatactctt ccagataatg gttatattct
     8581 ccgccccgat aataattttc aatcaaccag tcgacctttt cagccagtgt ttccatcgac
     8641 atcgtcatct catcggagtc acaataatcg catgattctg aggcatcggg atcgcttgcg
     8701 atgatgttgt tcagataacg gtcatcgttc agacactgcc tgcaaattct ctttgaaccc
     8761 ggcactctgt ttaatcctta ggttagatag atttcgcatg cgaaatcttt cggtttaatc
     8821 acgtcgatgc agctgtatta gagcctcaga aggacgatag tgctgctttg cccaccgccc
     8881 gtaattatta acggactcat attgacgttc tgcagcacca tcacggtaat acagtatatg
     8941 gataatccca tgtagctgca gataactggc tacagtggtt acattattac gaacgaagcg
     9001 cgtgtggctc tggaagatat caagatcaac tccgtcctgt ccgtcatcaa gcgcatcaag
     9061 aataaaagcg attaatgcag aagcgataga acgtctgcca gagactgcga tacaagcatc
     9121 aagctcttga aaagtcatct ggatttcctg ttgtaacggt agctggaatc ctcttcaatt
     9181 gcagccacga accatccggt agcagtttgc ctgtctatgc tatcaggcaa ggaagcgact
     9241 aactgctcaa gtggaagtgt acgattggct ctgaatatca gcgccgacat accacaccag
     9301 tcctggttac tttctggctt ccagccgatg cttagcatca atgttaacgc cgtctgggct
     9361 gcccacaacg gagtttctgt cgggttagag gcccatgaaa gtaatgccgc gccgggtatg
     9421 ggtctgtttc ttgtaacgta tcctagattg atagccgctt ctgttaatgc ggcggcagct
     9481 tcggattggg ttccgggata tcgaagcgcc cagaggcgga caatttggcc acggaatgca
     9541 gtggccattt cggtgaacgg ggttgatttc atcacagaga gcgcatccct ttgacgcgta
     9601 gttcagaagc ctcgactgaa atctcaccaa tttccagttt cacccacaca taccccgctt
     9661 cgtctggcgc gcggtcagtc attatcaccc cttgctgacc gtctatctcg acaacaacct
     9721 gccaggtttt cacatctgaa ttgctgtcca aaggcttggt tggctttttg ttctgagcct
     9781 tagatttcgc atgcgaaatc tcacctttgc ttttgttctt aactgcatca agaacagctt
     9841 taacctgcga acgtgaaata ccagactctc gcgcagctgc gacaagcttc tcggcctctt
     9901 cgggagactt ttcatttacc tgtatcagtg tgtatgccag atcgatatcg gatgtgactt
     9961 tatcccggag aagggcggta acattttctg gtgcatcaag gagacgaagg tgtttactga
    10021 caaatgaggt aggcttaccc attgtttttg cgatttgctc acccgtcata ttgaactcat
    10081 gcttgagctc atagaacgat tttgcagtcg ctaatggttt caaaccagac tgctgcaggt
    10141 tttcaatgag ctggccgatg atgcgttctt ttctttcccg tggtggttta ccaacataaa
    10201 tatcaatatg ggtatcacca cgtttgcgag cagtccgaac acgcgtttcg cctaaccata
    10261 tctggtaacc acgtctgtca cgaggataac aacgaggcgg ggtaagcatc cctatcgttt
    10321 cgaaggtatc agacatacca tcgatgatat cttcctcgaa gtcttcttca ggtcgcactt
    10381 gttcttcagc ataaaccaga tttatatcta cgcggattat ctgtccatca ggttcgatat
    10441 ccgggcgggc aatatccgcc ataatcttgt gcgcagctat atcatggctc tcctcatctg
    10501 gctgtttatt tgcaatgacc tgttcaatat cagaaacaac agcagcattt tttttcatta
    10561 cctgctgcgc ctggagtctc ttcataaaag gtttcttggc cattacttac ctcctgcgag
    10621 cataataatt ttctgggtaa gttctttata ctgaattgca ggaagacact tagggtcata
    10681 ttcatcaaag gagagaccaa gttcgatcgc ttcttttgca gcagcaggta caaatgatat
    10741 ataggcgaga atgtattctt cggaatagcc ttttccttga agaatctcga tgaggagatt
    10801 tagattcagt gtatcaatag gcccaagata tccttcttta aggggttttt tataattctt
    10861 tacctgcgtc tgagacacat aagtccccat gaattgcagc gaaggattat agttccgctt
    10921 aattttctgc atataagtaa gctgtttacc aactgcatta cgggcctgct tctccagcag
    10981 ggtagggata agaaggtaat cagctgacat atgcgctgcg atcataacat ttgaatatga
    11041 tggtgctgaa tcagcaataa taaaatcaaa ctgtttcttc agttcttcaa tacggtccct
    11101 gaagtcgaac atgcagtcgc tgttgcggta gttaatttca ttaagttcac tggttgcacc
    11161 tataaagaaa cgcccgccag gaagttcaat cggtcgaacc tctgtttccg gaaagaaaag
    11221 ctgatatgta ttcgcctcgc cgggtgtaaa actgttactt aaaggttcgc gttctatctc
    11281 ataaggtaag tcatcgggga aagctctctc cgtcagacca gttgtccagt ccatatcaat
    11341 ttcaagcacg cggaaaccag ctttacgaag atcatgtgca atttcactgg atgactgcga
    11401 tttaacgatt ccgcccttct gactacatac aacgatcagt tttccgatag gtccaatttt
    11461 cagtgtcatg taacagtcct taagatttcg catgcgaaat gtttgagaag gggcggcaca
    11521 gcttgaaaga gtatttcaat aatgaaatga attcattgcg ggatttcata cacactcctg
    11581 tgagtcaggc gtttcgccag gtgcagaagc agctatctgc cactgcgttt gttgtcgtgc
    11641 tttcatgacg acctaaacag tatacagaga aacattaagg tatcaattat gatatgcaat
    11701 agaattcgca tgcgaattct attatgtgag aaaggtgata tggctgacga taaaagctgc
    11761 accaaagaaa tggacgatac cgcagagaga ctggggtatg gcaatgagcc gttttaacat
    11821 cgagttcggt gaccgcctga gcgatcacct ttaacgagtg ggcagttaca cagaattatg
    11881 gacaccctca gattggccaa aaatctcaaa cctactgata atattagcta ttagctcacc
    11941 tatcttgttg tggaggtgag catgcccagt atcagtgcgc tactacaacc cgctatagcc
    12001 cttcttcatc ctcatcctca tcctcttctt catcttcatc tacgtagtaa taaccatcac
    12061 caccacaacg aggacaaacg tcagcgtttt cttcaccttc tccgtaacaa gtatggcact
    12121 gcgcgaacgg attatcatct tgtgggtcat aacctgaacc attgcagtta ctgcacgtaa
    12181 taattacatc accaccacgc ccatggcata atgagcaaat tacttttttc atgatctatc
    12241 tcacttttgt gtatacgaac agtccacagc ccaccaacga accattttat gtacggatga
    12301 tgctgtatga ttgcctcaag agtaaaaact cataagagta ggcatgttaa tcatcagcaa
    12361 tttagttagc cactcgaatt atttttccac ttatgtattg caattctcga ggaaatgtga
    12421 atgaaagaaa ttgttttaaa gttaagtgag gctgaaaatg tgttgagaga atggtttgaa
    12481 gctggaatag ccttcaattt aatttttgga cctttacatt ttagaaaaga atctggtttg
    12541 gttcacttaa gaaaatgtct tgccaaaatc cctttagccc tcaggcctca gtactatgac
    12601 atattagaga aagcatttag cccacgtcat aacatattag atatactttt taggaataat
    12661 tatgattatg attcattgat gttgagaggt cagttgtatg catatgctga atgccttact
    12721 aaaaactacc ccaaaatgcc acttaaatta ttacttactg cagcagcaac tcctcattcc
    12781 gtcttagaac caaaaaaaat tattcatgct tactataaag ttcgtacaga attagaaagg
    12841 aatagtcgcc agaaactaaa cattacaatc gtagatccta ctctaattgc gttatgtaaa
    12901 ttagttagtg aacggcaact tacatcaaac ttggttgaca tcgagtatgg taaccctcaa
    12961 ggaaaaatga ctccatttag gattcactcc ttcgatttat tcactaataa ataccgcagg
    13021 ttaagtaatg aaaaatttag tcttgatcag gttcacgggc actttataag tatagctcac
    13081 aagcttgctt taggtcgaga ccctttaaac gaggtatcgc acccactact aaaagacaaa
    13141 aaatatacgc agtgggcacc aatattacat gcattatgtc gaaagcatga aaatagttca
    13201 caggtcgaat actacaaaaa atatagcaaa aaatttccgt tgaaatacaa acatgagttt
    13261 gacagcaata gtattaatca tcagattgaa aaattaaata agcgatattg ttctctattt
    13321 cgattcctga aacctagccc cgaaaacttc tctcaaaacc aacgaaatgc actaaagacc
    13381 acgccccctg aagtgatgca gaaaatgata gtatatcata tgattatgtt ttatttttca
    13441 cttataaaaa atgctgcttg gtacattaaa gtaagagatt ttatgataag cctaaaaatg
    13501 agctatcctc aagactatgc atcaaaactc ttcgcctttt ctagtggaga tgaatgtatg
    13561 gatgatacat tatacaatag ttttaacgaa atattcagtg ccaatccagt cggccttttt
    13621 ccctggatgt tttcgggtct actaccagaa ccgatggagt taatgacgca ttatttcagc
    13681 aataaaaaaa acaaagacat tgagcatata gataaaaaaa acaagtcctt tagaaatatt
    13741 gatttagcag cttctgtgct aataatacct aaatttctta acaatttaga tcgtgcgaaa
    13801 gggataaatc ctagtattat ggttaaacta ccctcaaaca attcagaaag ttgtattttt
    13861 tacacagcca caggcatacc aaaagaagaa ggattatatc ttgctgagct gtttagtaag
    13921 ggattgtata ttcaaagaaa tatagaagaa agtttaacga tggaactgag agaaattgaa
    13981 gatctactac tcggaatttg tttactttgg catgaaagct ttgtaggtaa aatatcactg
    14041 agcaagttcg ttaatatcct acaacaaaat gaaattaatg atattagtga aagaacttta
    14101 aaagcaagaa aagataaagc caaatattgg cttatgcaat ggcctagtca gcttccctta
    14161 atctcctgag atatagaatt atgtctccaa ttagttgaaa atatactttc gatgaatgag
    14221 tcgatagctt tttcgtggac cttgatggaa cgtgagaagc aaatcgtttt gcgggccaca
    14281 cgcttaatgc gagtatgggg tgtcaagtgg ctccttgatt aaacaatcct agaccgctcg
    14341 ctagcatatc actttagggt atacgtcaac gataccggga gcttcctccg tttccggaat
    14401 gatgtgacca gatatgtgag gccataaacg gcaagaccac ttaaatgtct tcagagacgt
    14461 ctgtaacttt aatgtgaatg gtatgccagg gaagaggata gggggacaga accaccccgg
    14521 cactgccccc ccggaagaaa cagaaagtcc cctccacgcg aatgttagca ttttttgcct
    14581 gtgatattat gacaacgtta ttttcagagc atgggccgat gaaaaagaaa accaccacgc
    14641 ctacacccca cgatgcgaca ttcaggcagt tcctgacgca gccggaaata gcccgggatt
    14701 ttatggagtt gcacctcccg gcagaactgc gtgccatctg tgacctcacg acgctgaagc
    14761 tggaatccgg ctcgtttgtt gaggatgacc tccgccagta tttcagcgac gttctctata
    14821 gcctgaaaac cgccggcggc gacggctacg ttcacgtgct tattgagcac cagtcgtcgc
    14881 ccgacaaaca tatggctttt cgcctgatac gctatgccgt ggccgccatg cagcgccacc
    14941 tggaggcagg gcataaaaaa ctgcccctgg tgataccggt gctgttctat acgggtaagc
    15001 gcagcccgta tccgtactcc acccgctggc tggatgagtt tgacgacccc acgctggcag
    15061 gaagtctcta cagcaatgct ttcccgctgg tcgacgttac ggtcattccg gatgatgaaa
    15121 tcgccgacca ccgcagtatg gccgctctga cattactgca gaaacatatt caccagcgtg
    15181 acctggcaga actggttgac cgcctggcgc ccattttgct ggccggatat ctgtcttcat
    15241 cacaggtcat atcgctggta cactatatcg tgcaggcagg cgaaacagcc gacgccgaag
    15301 cctttgtacg cgaactggca ctgcgtgtgc cgcaacacgg ggacgcactt atgaccatcg
    15361 ctcaacagct tgaacagaag ggcatcgaga aggggattca gctcggtgaa caacgtggta
    15421 tcgaaaaagg ccgcaatgaa ggcaagctcg aagttgcccg taccatgctc agcagcggta
    15481 ttgaccgcag taccgtcatg aaaatgaccg gtctgacgga agaagacctg gcgcagatac
    15541 gccactgatt catccctcct gagaacgcgt ttactgcccg gatgaggcaa agtacctacc
    15601 cggcatttca ttctgaaccc cttgtctgtt cagaactcgc ttctggacag acaaggggtt
    15661 ggacaagcct gctgcgcgtc agcctgtagc cgttaaatcc gttctttcag catcgccacc
    15721 gcctcggtcc ccggcatctg gaactgaaca cggtggcgtg ccgctacatc gagcgcaaac
    15781 actttcgtat agacttccgt cgaacttatc gacttgtgtc ccatgagact ctgcagcacc
    15841 ttcagcggta taccggcata caacatatgc atggcgtaac tgtgcctgaa cgtgtggggt
    15901 gtcaccggta ctgagaatgt cacaccgtcg gcggccgcgg cttcgaccgc ctcagcaaac
    15961 caggtgcgca cggtccgatc ggtgatctgc cagatgcgcg ctttttcggt tctgccggtt
    16021 cgtttgtttc gtctttccaa aggtattttc agcgtggcca ccatcatctg cagctggctg
    16081 acgtactggt gatcggagag cggaaccaga cgatggactt gattgccggc gggcgctctc
    16141 ccggccgttc tcgccgcttt ctccgcacgt tgtttgagcg tggccagttg cacaaacgga
    16201 tacggcggcg ccagcgaaaa atccccccgg gtcagcgcga gcgcttcatt aatgcgcgcg
    16261 ccggtgttcc acagcgttgc cagcagcatc ttgcggtgaa gatccggcac gtaatggagc
    16321 agggcgctga cttccggcgc cagcaggtat ttcgggagtt catcctgcac gagcgccatc
    16381 tggcgcaagg cgagggcggc agggtagtcg atagccaccg gcagctgcgc tgcagttcct
    16441 ggttgcggtc ccaaggtaac gagcccggtc atcgagatac atcgctgtct gttgatatgt
    16501 tcagcgccga agcaataatt tcccggtttt cctcactacg ccagtttccg aaatagtcta
    16561 cttcttggct cagggactgg tgtgccgcct ctataaggtc agacactctc tgcagagcat
    16621 tctgtaactg ctgcgctgat atcactcttg gcacatcatg ctcatccaca ccattacggt
    16681 gcactaggtc gtttctccag tctgcgatga tacccagcgg cctaaccggc cagatcggcg
    16741 ggatgtgcag cacggcgctg aaatagcgct caattgtttc aacgttatgg aaagtcatac
    16801 gagcaacgac attccttgca acagggcgaa acctggaaag cggcatcttg ctggtagcca
    16861 gcttctcttt cttgctggcc ctggcaaaag gtaaataata cgcctcaaaa tatctctcga
    16921 gcggccagtc atgctccagc agggccttgg cgcagtacat caggtacgcc tccatgactg
    16981 ttacggcatg aacgaaagac atcctgtaca caatttcagg cagctctctg tcgagcaggg
    17041 catatagctt cttcagctcc tgtcttgcgt gccggtaacg ctggtgaaca tcattcaggg
    17101 aaaccacgaa gagcccgtgt tcatgatccc actgagcctg atcagcaagt gcctcctgtt
    17161 cccagtaata caggttcgcg gcctgctccc attcttcaga ttcggggaca acatccgggt
    17221 aattttcctc aagccactca ttaaatcgtt cagcttcaat atcaatcatc atgtctttca
    17281 tgatttccat agccccttta ttgttgagaa atcccgccag cctttttaat tcactcgtcc
    17341 atagccaaga atggctggaa ggtttccatt tcattcaaga gctcgacaaa atcctcccgg
    17401 gtcggatctg gtacttcatc cctggcctca ttttttaact tacgcagccg ccagcctttt
    17461 ccctttccat tccagatagc aagaacgtcc ccggcaggat tcaaaaaatt tcgtgagata
    17521 taccctgctg gccatcttcg tgatgaatca cgcagccaca aaactggatg tgttgctacg
    17581 aggtggtcgt atatacagtg aatagaatga aaataattag cctgaaaact acatgaatta
    17641 cacataagca tccctttcaa atagtcactc agagtaatta tgacgggtgg cagatctgtc
    17701 tgcgcacaac cctgtgtctg tcacccgaat gaatttcacc tgctgtcatt attttcaggc
    17761 tgataataag cagcattata accacgctat taaattatcg cattcccgtt tccgtcagat
    17821 ccgactttca ttaatatcac aactttccgg aagtgtacca cgctgagaaa atcatcatat
    17881 cactgttttc cgcccctgcg cggcgcatca caggtcaggg agatggcaca acatatagtg
    17941 tcattaacag ctaaattcag cactacatgt tgtgctgacg ggagatggcg ctgtgcggcg
    18001 ggagaggaag atccgtgact accttttttc ggatcttatt agtgtagaag aacgattgtt
    18061 gcggtgaggg gattattgct taggaagcaa aattcaaaac tgactaagcc agaaatagca
    18121 acaacaagat aaggaaattt tccccctcag cccctgcatg ccgtgagggg gagcaactac
    18181 ccaactacag ttttcatttt atgatacgtc ttcactaggt aatacctgtg aactgacaga
    18241 gtctttacgg agtgtatggc ggacctaaat ttgatccctg aaaactgtca gcgctgcgag
    18301 gacgaagaaa gtattacggc taaagccgta atacctcccc atccagcttc aagctatgta
    18361 gaaacttatg tgggtagagc agtcccgaca gggcagtacc gcattgacag gccgtcaggg
    18421 ccgcttacga gcttgtatga caaggggggc ctagcgaatt attgtacggt aagccttttt
    18481 tgaacagcct tacctattgc tatagagggg aactcctacg ttatacgccg ctttcagggg
    18541 attcgacctt aatgagacaa ctcaaatatg atcgacaact taaacgcgat agttgctgat
    18601 aaaatcaatc cattacgcgt ttcaattgat taaataatgc acttaatgta caaaaattcc
    18661 cgatctcaaa actgaaccct aaagatacca ggcatctccc ccaggaagcg ccctcatgcg
    18721 ttttcctgtt ccgcccctgc cacctacctt ctgttacata cttgttgtat tattgttttt
    18781 gttttcaatc cccaaggaga cgtcgctata tgactgtgcg ggagacagag gaaaacattg
    18841 aacaaatctt ccctgagctt tcgaacttaa ttgatattat taataaatgg ctttcccatg
    18901 gaaacattga tcctgaactg gcacacttca tcatttatct tcttgattac ctaaccacca
    18961 cacacaaaag catataaaaa atttccttgc acatccattt ttttgcgaat attattattc
    19021 ctgctgaata tgattcaata tcgcacgcta ctcatccatc ccaggataat gagttcatag
    19081 gttgaagtct caacaccccc actacggggg tgtttttttt accaataaac acaaatcaca
    19141 ccatcgccat actctgtttg ccagacatcg attggtaaat atgcgtaact cgctataata
    19201 caaaaagaaa aatcagaacc agaaaccagt ttccagataa aaaacaccct cccgaaggag
    19261 gggacagaca cattcatatt tcccaaaaca ggaattttta tttttatgac gcattaatga
    19321 agttacttca tctccagtag attaacttca tttaaagtgg cgatcaatca tatttattac
    19381 gactctaaac tattacaaca accacaataa gatatcacct caaatataaa agataattct
    19441 tacaaaaacg ttccgaaaac aaaaaataca gataaacacc aattaattca gagcaatcta
    19501 ttgcgaagaa tcagagtaac agggccccat gatataatca tggggccctg ttgcttttac
    19561 agatgtaaaa acgaccaatc atttaacatc tgttatcggc accgccccgg acgggttcgg
    19621 cgcaaatatg gtatggtacc agcccggcaa ttcacctgcc gtgcaatctg acgaagcagt
    19681 accggcgtat gcagatatat cgtccagaca tatctgccgc attcaggcgg tgagtaaagc
    19741 cttctctctc aaaagaggag caacagttat ccacagattc tggggataac tgcgaaaaga
    19801 atatgtgcca ggaaagtcgg ctcccgggaa aaagaccgct ggcggcgcac ctgctggata
    19861 cgaaagctga gctgaaagga tcccgggagg acgtccgtaa acttcagtcc ctggcccgtc
    19921 atgacgggaa ggcgaaaaag tgaatgcgga cacctggacc cgcctgcagg ccttccgaca
    19981 tgctctggag ctgacttcct gtgaggcttc tctcaccgcc gggtatgatc acctgaagga
    20041 tttcccggcc ggttgcagcg agctggccag ccagacgctg actgattatc tgacggaaga
    20101 cggcagcaac ctgtactcct gtattgtcgg tatgcagtgg gataatggtc ctggcagata
    20161 cggccacgtc attgccgacc ctgccaggga ctatatcgac ctaacccttg atcagtttcc
    20221 aggctatcat aaccggattg tggcagagcc cgttgagtcc ggcggacagc tcgcggcaga
    20281 cctcaacagg gagcctgcca tcagtacagc tgatggaata gtggccagcc catcagataa
    20341 ggttaattac attactgaac agaaaaatcg ccagccaatg gtgatcatta cagaatgtta
    20401 caagctgatc agaatgacgg caagatttat accaatgtgc cagtcatctc aactcttcca
    20461 gcaccaggta ttccctcgct tatttatcat ttcagacgtt ggctcctgca gtttgttcag
    20521 gaacacctta gatcgtttag ctactgactg gaaggtgaca ctgtccatcc tgtcgctata
    20581 gggcttgata taaacctggc aggcaaggga tctggttaac tcctttcctt caataaagtg
    20641 tttgaagatc cagagatacg tatccacaag ctccagccct gtactctgtg tccccgactg
    20701 gaagacctgc ggttcagctg gtatttccat tacatccatg accggtgtct gcgcagcagt
    20761 gcctgtcatc aggatcctgt cactctttcg accgacagat tatggcatac agttgggctc
    20821 gtaagatcca gagtcaataa ctgacagggt ttattctgtc atcctccggc agcaaaggca
    20881 tatacgatat tcacttattt tgtcggtcca acggcttttt acagtagctc gttttgccca
    20941 actgatgcta cagctctttg gtttgcttca tcacggatat cagctccgac acaggcacag
    21001 ctgactggtt gcaacgtctg cacttctctg gacctgaaac gtcaattatc aggattctga
    21061 cgattagtct taagactgtc ggtaaattaa gtgatgccag ttgtcaggat attcatggag
    21121 ccagtctgca tgcgcctctg gcgttgagac aaacatatga agtgtatttc ggatatgact
    21181 gtacgctcag gaagtacggc agattccgtc aaaaattacc tgtaaagaca gacttcagaa
    21241 aaaacagtgc tcgccgcaga ccggtgaccg ggcgaagccc gcgaaccggc gaggaagcgc
    21301 aatatatctg tagttacagt gagcaggtac gccctgaagc cttcataatc ggtagtgtat
    21361 gttgtttttc cggaatgatt tatccacata tccagaggcc cgatccagta aacagatcca
    21421 tgaatgatca acaaaggatc cattaaagat ccccatatcg ctgcaaacct tgtcactcat
    21481 gggccgggac cacgttcaca taagctgtgg catgttatgg ataaactgta acaggttcat
    21541 gataagctgt attcagtaat gcatatactg aagtaagtta atgacataaa ctatggtcag
    21601 tacgccagac tcagttgtta aatacagact gcaggttttt cttcagtcag ttgacggggt
    21661 tctgacacac gattttctgt ttattctttt actgtccaca ggcaggaggc tttctggaaa
    21721 acgaaaattc agacatcaaa aaactgttcg gcgag
//
