LOCUS       CP095473                9111 bp    DNA     circular BCT 18-APR-2022
DEFINITION  Klebsiella pneumoniae strain 2017HL-01181 plasmid unnamed, complete
            sequence.
ACCESSION   CP095473
VERSION     CP095473.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN26849763
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Prussing,C., Southwick,K., Snavely,E., Kidney,A., Randall,L.,
            Sossei,A., Dentinger,L., Shushe,O., Fernandez,R., Haas,W.,
            Lapierre,P., Singh,N., Nazarian,E.J., Musser,K.A. and Mitchell,K.
  TITLE     Comparative analysis of multiplexed PCR and short- and long-read
            whole genome sequencing to investigate a large Klebsiella pneumoniae
            outbreak in New York State
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9111)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2022) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 9111)
  AUTHORS   Prussing,C., Southwick,K., Snavely,E., Kidney,A., Randall,L.,
            Sossei,A., Dentinger,L., Shushe,O., Fernandez,R., Haas,W.,
            Lapierre,P., Singh,N., Nazarian,E.J., Musser,K.A. and Mitchell,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2022) Division of Infectious Diseases, Wadsworth
            Center - NYS DOH, 120 New Scotland Ave, Albany, NY 12208, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-NOV-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology             :: ONT MinION and Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/12/2022 11:27:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,785
            CDSs (total)                      :: 5,660
            Genes (coding)                    :: 5,489
            CDSs (with protein)               :: 5,489
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 70 of 171
            Pseudo Genes (incomplete)         :: 111 of 171
            Pseudo Genes (internal stop)      :: 23 of 171
            Pseudo Genes (multiple problems)  :: 28 of 171
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2017HL-01181"
                     /isolation_source="rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed"
                     /geo_loc_name="USA"
                     /collection_date="2017"
     gene            complement(join(7717..9111,1..93))
                     /gene="ltrA"
                     /locus_tag="MQ286_28875"
     CDS             complement(join(7717..9111,1..93))
                     /gene="ltrA"
                     /locus_tag="MQ286_28875"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003964 - RNA-directed DNA polymerase activity [Evidence
                     IEA]; GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="UPA38249.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            complement(569..2983)
                     /locus_tag="MQ286_28880"
     CDS             complement(569..2983)
                     /locus_tag="MQ286_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="UPA38250.1"
                     /translation="MSHDDMSNSSGFNEAAASFSWNGPKKAINPYLDPAEVAPESALSN
                     LITLYAADNEQEQLRREALSEQVWERYFFNESRDPVQREMEQDKLISRVKLAHEQQRFN
                     PDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRDSQL
                     STSFRFMASHEGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDVKPEE
                     IRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWYRKLWK
                     MRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDGDTLDME
                     DVVNASCSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNNGRPNPTW
                     KNATVRQSSDYLVDMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCFMRKKDRRA
                     ITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNIDGRGLAGEIS
                     KETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAARQQGDKKAGVP
                     VLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEPTAYGDHGIRIY
                     GIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCPLAENLNQQGKDK
                     SADGDTRTDITRMDDKELHDYLHSMSKKERRELAARLRLVKPKRRKDYKQRITDHQRQQ
                     LVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDDKWRNLY"
                     /name="replication endonuclease"
     gene            complement(2980..3837)
                     /locus_tag="MQ286_28885"
     CDS             complement(2980..3837)
                     /locus_tag="MQ286_28885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="UPA38251.1"
                     /translation="MSTILKWAGNKTAIMPELKKYLPAGPRLVEPFAGSCAVMIETDYP
                     SYLVADINPDLINLYKKVAADCEAFISRARALFEEANREVAYYNIRQEFNYSTEITDFM
                     KAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRKFAEKAQRATFICASF
                     DETLAMLKAGDVVYCDPPYYGTFSGYHTDGFTKDDQYHLASVLEHRSSEGHPVIVSNSD
                     TSLIRSLYRNFTHHYIKAKRSIGVSAGESKSATEIIAVSGARCWVGFAPSRGVDSSAVY
                     EVRV"
                     /name="DNA adenine methylase"
     gene            complement(3834..4061)
                     /locus_tag="MQ286_28890"
     CDS             complement(3834..4061)
                     /locus_tag="MQ286_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="UPA38252.1"
                     /translation="MADAMDLVQQRVEEERQRHIRTARAKTPGVSRVLCIECEAPIPPA
                     RRRAIPGVQLCITCQEIAELKGKHYNGGAV"
                     /name="TraR/DksA family transcriptional regulator"
     gene            complement(4061..4294)
                     /locus_tag="MQ286_28895"
     CDS             complement(4061..4294)
                     /locus_tag="MQ286_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 domain-containing protein"
                     /protein_id="UPA38253.1"
                     /translation="MRSIETLTTKTGPDDAGLNILLTEARLEDRRARAEAMAARLDSLA
                     CHITSRQLNHVEAAELLRVTAEAIQNEAQEIH"
                     /name="DUF2732 domain-containing protein"
     gene            complement(4362..4703)
                     /locus_tag="MQ286_28900"
     CDS             complement(4362..4703)
                     /locus_tag="MQ286_28900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="UPA38254.1"
                     /translation="MAIEGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFIK
                     DMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVVSL
                     FPRRLTMPN"
                     /name="DUF5347 domain-containing protein"
     gene            complement(4667..4867)
                     /locus_tag="MQ286_28905"
     CDS             complement(4667..4867)
                     /locus_tag="MQ286_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2724 domain-containing protein"
                     /protein_id="UPA38245.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWIMGEDGKRWHPCRSQDELLA
                     ELSTKKQGNKWLLKALRRLFH"
                     /name="DUF2724 domain-containing protein"
     gene            complement(4875..5384)
                     /locus_tag="MQ286_28910"
     CDS             complement(4875..5384)
                     /locus_tag="MQ286_28910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="UPA38246.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLNP
                     AQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAEIG
                     RVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTVTGLG
                     ASFGLL"
                     /name="phage regulatory CII family protein"
     gene            complement(5417..5638)
                     /locus_tag="MQ286_28915"
     CDS             complement(5417..5638)
                     /locus_tag="MQ286_28915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="UPA38247.1"
                     /translation="MTPNISITLNTPHVTIERYSELTGLSIDTINDMLADGRIPRHRLR
                     KDKKREKVMINLAALTVDALTDCNVVFN"
                     /name="regulator"
     gene            5784..6662
                     /locus_tag="MQ286_28920"
     CDS             5784..6662
                     /locus_tag="MQ286_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0045892 - negative regulation of transcription,
                     DNA-templated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UPA38255.1"
                     /translation="MQNRGGQSVIARILKAYGFSSRQAFCNHLGISQSTMANRYARDTF
                     PADWVVICSMETGVPVEWLAFGTDTEKGRITNNAEKSHNNCDSKHQHLNREQDIQNENF
                     FTINQGGKAAIERIVLAYGFKTRQALADHIGVSKSTLANRYMRDTFPADWIIQCSLETG
                     ASLTWLTTGNGAMFEKPRINTITIPYHKIIDGSLAQETVLTFDSKLLEGTFLQPLAVFI
                     DEEIYIVESKFNEVTDGKWLVNIEGKISIKDLTRIPVGMVKVVGTNASFECLLTDIIVL
                     AKCKRVFTKNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            6674..7618
                     /locus_tag="MQ286_28925"
     CDS             6674..7618
                     /locus_tag="MQ286_28925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UPA38248.1"
                     /translation="MTEPTNKDSEIKKHLLDLLNSQSENIAKHFYSHIKNLIEAGEFSE
                     AHNNLALIEKYMTRPQMDEEPNINENETNKRKNVKSLEPNNYVEHIIQLEERNSILTLQ
                     LEHYTQDLNRKNAIIEKNVKQVNSLISENKELRSQVQQQRIDEKIPTYVNDVKSDLASD
                     DKHFILMSIIWSIAGVFFGFLAVISAFFTLYMNLDLKNLTNLQLIYIFTRGLVGIAILS
                     WLSYICLSNSKKYTHESIRRKDRRHALMFGQVFLQIYGSTATKEDAIEVFKDWNISGDS
                     AFSGQTEQPPSFASFLNTIKDKVKVTGSDKETD"
                     /name="hypothetical protein"
ORIGIN
        1 gctccttcgc tccactgact ccgctccttt cgggttgttc gcctgcttcg ccgctactat
       61 gagcgagtcc gacttctcct ctccgtacat caccggctat gactcctcgt cttcccggtg
      121 cgggccatct ccgacactgg cagatggtca gaggggagat ctcccggttc ccgcgtagag
      181 atcgtattga catgccaggg tctcagaccc cgccgggtcc atgtggcact cgcagtatcg
      241 caccctatga tgttgccttc cgttaacagt acaacgtcgg cacccggtaa tttaatatac
      301 atttcgtggc tcaatggctg gcctgtcaac acccctgtca acgcttcgcc ccatacctcg
      361 cggtatgcaa cgcatgactc ggggaccttg tggattgctg gtccttcaat ggtcggggac
      421 tttcacccct tgatctctaa ccggtctccc ggcgcacact gtttatattt acagtatata
      481 ataatattac tgatgtaaaa cgttttttta cgttcatcag cctgatatgc ctggtattat
      541 taagagcacg aattgttaac ccgcgtaatt aatacaggtt tcgccactta tcatcttcct
      601 gcaaacgctg gttccgatag aagatacgca ggcctgctcc tgatggaata ctgccgccgc
      661 gaaggagtaa atcgacctct ttctcgctgc catcaaatcc tctggacttc agttcataga
      721 cgagctgctg tcgctgatgg tctgtaattc gctgtttgta gtctttacgc cgtttcggtt
      781 tcaccaggcg taaccttgca gccagttccc ggcgctcttt tttgctcata ctgtgcaggt
      841 aatcgtgcaa ctccttgtca tccatgcggg taatgtccgt cctggtatcc ccatcagctg
      901 atttgtcttt cccttgttgg ttcaaatttt cagcaagggg acagttattg ccacgagtcc
      961 aaggggcgca agcgccctgg tcggctgccg cctcctgaac gtcaacggcc ttacgaacca
     1021 ttttccactt cactgcatga gtgcagatct tgccctctgc aatgggtgac cagatgccat
     1081 aaatacgaat accgtgatcg ccataggcgg tcggctcttc gttaatttca taagcagttc
     1141 tgatgaggtg atatttacgg ggaaccagta cacctccctg cttcatgata taggtggcaa
     1201 aacaaccagc atcagcagca gccagaatgg catcaaggcg cgggttatcc agtaccggca
     1261 cacctgcttt tttgtcaccc tgctgccttg ccgcctgacc agccagcaat cgcagttcac
     1321 ggtaagcctg acgccccgga ataccaaaga agcggaattg ctgaacacga tgcaaagacg
     1381 cccaggcatt cacgtattca gcgttatcac gaagagattt acccgtttcc ttgctgatct
     1441 cgccagccag accacgcccg tcaatgttct tactgatgta tttcgctatg tagcttgtcg
     1501 gcgttccttt gcgcgggtta atcagctcag acttaaagcg cggccccgtg ttattgccca
     1561 gctcctcgcg gtcttcacgg atagcaaact tacgcaacaa tgcagtaatg gcgcggcggt
     1621 cttttttgcg catgaaacac aacaggtgcc agtgaactgt gccgtcgtga tgcggctcag
     1681 ccacccgcac gccataccag cgcaacccgg ctttgtgcat cgccttacga aatgcagcaa
     1741 acatatcgac cagataatcg ctgctttgtc ttaccgtcgc gtttttccag gtcggattgg
     1801 gtctgccgtt attgagcgtg gaatggaaac gtgacggaca ggtaatggtg tagaaaacgg
     1861 cgcagtcacc gcgcatttcc gcgataagct ccagaccttt aacacaggcc atcatctcat
     1921 tgcggcgatg cgccgggttg ctgcagctgg cgtttaccac atcttccatg tccagcgtgt
     1981 cgccgtcttc gttcaccagt tcatgagaac ggaaaaactc cagcgactta cggcgctgct
     2041 cacgtttatg catcacagct tcatagctga catagggaga tgcttttttg ctgaccaggc
     2101 agacagcacg caactgctct tcccgccatt cacaacgcat cttccacaat ttccgatacc
     2161 accagtcggc gcacagcata cgcgccagcg agcccggaat gagttcatag ggcactggtt
     2221 tacggcggtt tcttttccga cggagttgct caaacgcagg cgggatgaca tccaaacgta
     2281 gggtttccgc tgccaccttt tcccatgtct tgcggatttc ttctggctta acatcatcgg
     2341 tggcgtacaa atcaccacaa gcggcatcaa gacacatgct catatgcgca gcgacaaggg
     2401 tagacaggcg tttcacctga tcctgactca tttcaggcag gatcagcagg ccgtccagcc
     2461 cttcatggct tgccataaag cgaaaagaag tggatagctg actgtcgcgt acatgctcca
     2521 gtcgttccag acatggctta atcgtctcac gcaaatagcg ggaataagcc tttggcctgc
     2581 ccaggctgct gaagtattca atacgttgca tcagcggctt gctgatatgg gaaggctggg
     2641 cgttgacgtc agccagaatg accatgtctg gattaaaacg ctgctgctca tgcgccagct
     2701 ttacccggct aatgagctta tcctgctcca tttcgcgctg gacaggatca cgggattcat
     2761 taaagaaata acgctcccag acctgctcac tcagtgcctc gcggcgcagt tgttcctgct
     2821 cgttatcggc agcatacaga gtgatcaggt ttgaaagtgc agactccggc gcaacttccg
     2881 ccgggtccag ataaggatta atggcctttt tcgggccgtt ccatgaaaat gatgcagcgg
     2941 cctcgttaaa gccgctagag ttgctcatat cgtcatgact catacacgca cctcgtacac
     3001 agcagaacta tctacgccac gcgaaggagc aaatcccacc cagcagcgcg ccccggaaac
     3061 agcaatgatt tctgttgcag atttactctc gccagccgac acgccgatgc tgcgttttgc
     3121 cttgatgtag tggtgagtga aattgcgata cagcgaacga atcagggatg tgtcactgtt
     3181 agaaacaatg accggatgac cttcagatga tcgatgttca agaacggatg ccaggtgata
     3241 ctggtcatcc ttagtgaagc catcagtgtg atagccggaa aacgtaccgt aataaggcgg
     3301 atcgcaatac accacatccc ccgccttcaa catcgccagc gtttcatcaa agctggcgca
     3361 gataaacgtt gctcgctggg ctttttctgc aaatttgcga atttcttttt cagggaaata
     3421 cggattttta taattcccat agggaatgtt gaaatgcccg ctcttgttat agcgacataa
     3481 cccacggtaa ccatgacgat tgagatacag gaaatatacc gctttcatga aatcagtaat
     3541 ttcagtggag taattaaact cctgccttat gttgtaataa gccacctccc tgtttgcttc
     3601 ctcaaataaa gctctggcac gagatataaa cgcctcgcaa tcagcagcaa cctttttata
     3661 gaggttgatt aaatcaggat taatatccgc aacaagatag ctggggtaat ccgtctctat
     3721 catcacagca caagaacccg cgaaaggttc aaccagtcgc gggccagcag gaaggtattt
     3781 tttcagttct ggcataatgg cggttttatt acccgcccat ttcaggatgg tgctcataca
     3841 gcacctccgt tgtaatgttt gcctttcagc tctgcgattt cctgacaggt aatgcaaagc
     3901 tgcacacccg gaatggcgcg acgtcgtgct ggcggaattg gtgcttcaca ctcaatgcaa
     3961 agcacgcggg atacgcccgg cgttttggcg cgggcagtac ggatatgacg ctggcgttct
     4021 tcttcaacgc gctgctgtac gagatccatt gcatcagcca tcagtggatc tcctgcgctt
     4081 cgttctggat tgcttcagca gtcacacgca gcagttctgc cgcttcgacg tggtttagct
     4141 ggcgggatgt gatatgacac gccaggctat caaggcgagc tgccattgct tcagcccttg
     4201 cccggcggtc ttccagacga gcctctgtca gtaaaatatt aagccctgca tcatccggtc
     4261 cggttttagt cgtgagggtt tcaatactac gcataatcaa ttctcctgaa tttagataaa
     4321 gggatgcccg gcgggtttac gccatgaatt tcattagttg gttaattcgg catggttagc
     4381 cgtctgggaa ataagctcac cactgcacga aaatgattca ttgctttaat cagctcccgc
     4441 ttttcgtcag tggtcagctc attaatgctg atgctatgac gttcagctgg gatttttgcc
     4501 ataaagaata tggcagccag tgcccgttta ttttgttcat tattgatatc ccgtggatca
     4561 cgcatatctt taataaaccg ctcaagctct gactcaatat tcaggccaaa tactttcgcc
     4621 cttaattccg caatgtgatt aagtccgttc aggcgttcac cggggcttaa tggaacagtc
     4681 gccgcagcgc cttcaatagc catttgttcc cctgtttttt cgtagatagt tctgccagca
     4741 attcatcttg tgaacggcac ggatgccagc gtttaccatc ctcacccatg atccagccgt
     4801 gcccgtagtg cattgccgga ctttgtttta ccagcagcga tgcaaatgat ggttctttcg
     4861 tcagcataag cacctcacag caaaccgaat gaagcaccga ggccagtcac ggtatcaact
     4921 gcactcgcca tcgcaggatt agcctgtaaa cgggcctgca atgaaacagc ggccagcgcc
     4981 atcagtcgtg ttacagagtt aatgctgctg atagcatcac gacgacctgc actggttttt
     5041 acatcgccag ataccgcacc tgctgcaaca cgcccgatct ctgcggttgc actcatgacg
     5101 taatgtggca gtttctcttt tgccacctca ttaatcggta cacatggcag acaatgaatc
     5161 tgtgccagaa aaccatctac cagcgttgaa tcttcagtca gatcggtaag cagccagatt
     5221 tctggtgcgg ttaataaatg aggttgagct gggttcagct tgttccgcag aatctgcaca
     5281 ttcatgccag cacgttctgc cagttgcacc aggttgtggc gcaatgcgaa tgcacgacag
     5341 gcttcatcaa aatgtggatg tttggaaact tggtaatcaa acatggtcga cacccctgat
     5401 gcatcccaaa atggaactag ttgaatacaa cattgcaatc agtaagtgca tcaacggtaa
     5461 gagcagcaag gttgatcatc accttttctc ttttcttgtc tttccgaagg cgatgccgag
     5521 ggatgcgacc gtcagccagc atatcgttaa ttgtgtcgat tgaaagacca gtaagttcgc
     5581 tataacgctc aattgtgaca tgtggcgtat tcagggttat tgaaatgtta ggggtcatga
     5641 tgcaacatct cctattggct tgtggtgagt cagttttaat cgtggcttca acttcacatt
     5701 tcggagaata ggatcacaaa tcggttatgt caacacacga aatcacattt cgccatgtgg
     5761 acgaaaaaaa gaaatcctta atcatgcaga atcgcggagg gcaatcggtt atagctcgga
     5821 tactgaaagc ctatggtttt tcttcccgac aagcattctg taatcaccta ggtatatcgc
     5881 aaagtacaat ggcgaacagg tatgcccgtg acactttccc tgctgattgg gtcgttatct
     5941 gtagcatgga gactggagtg ccggtcgagt ggttggcatt tggcactgat acagagaagg
     6001 gaagaattac aaataatgca gaaaaaagtc acaacaattg tgacagcaag catcaacatc
     6061 tcaatagaga acaagacatc caaaatgaga acttttttac tattaaccaa ggcggaaaag
     6121 cagcaataga gcgaatcgtt ttggcttatg gatttaagac aagacaagct ttagctgatc
     6181 atattggtgt atcaaaaagt acattagcca atcgttacat gagagatacc tttcctgctg
     6241 actggattat tcaatgctca ctggaaactg gtgcttcatt aacatggcta accactggta
     6301 acggggcaat gtttgaaaag cctcgaatca atactatcac tatcccatat cataaaataa
     6361 ttgatggatc tcttgctcaa gaaaccgttt tgacttttga ctctaagttg ttagaaggaa
     6421 cctttctgca acctttagca gtattcattg atgaggaaat atatattgta gaatcaaaat
     6481 ttaatgaagt tactgatggc aagtggcttg tgaatattga agggaaaata agcatcaaag
     6541 atttgactcg catacccgtt ggtatggtta aagttgtagg cactaacgca agttttgaat
     6601 gcttacttac tgacattatc gttttggcaa aatgtaaaag agtttttact aaaaatgtat
     6661 aaagagaaac atcatgactg aaccaaccaa taaagatagc gaaataaaaa aacacctatt
     6721 agacttgctt aattcacagt ctgaaaatat agcaaaacac ttctactctc atataaaaaa
     6781 cttaatagaa gcaggagagt tttctgaagc tcataataac ctagcactaa ttgaaaaata
     6841 catgactagg ccacagatgg atgaagaacc caatataaat gaaaatgaaa ctaataaaag
     6901 aaaaaatgta aaatcacttg aacctaataa ttatgtagaa catataatac aattagaaga
     6961 acgaaacagc atattaactc tacagttaga gcattatact caggatctta atagaaaaaa
     7021 cgcaataatc gaaaaaaacg taaaacaagt taattctttg attagtgaaa ataaggaact
     7081 ccgtagccaa gtacagcaac aaagaatcga tgaaaaaatc cccacctatg ttaacgatgt
     7141 taaatcagat cttgctagtg atgacaaaca ttttatattg atgtctatta tctggtctat
     7201 tgctggggta ttttttggct tccttgcggt aatatctgct ttttttacat tatacatgaa
     7261 cttagattta aaaaatctca ctaaccttca gttaatatat atcttcacgc gaggattagt
     7321 tggaatcgcc attctttcat ggctatcata tatctgcctt agtaactcaa aaaagtacac
     7381 acatgaatcg atcaggcgaa aagatcgtcg acatgctttg atgtttggtc aagttttttt
     7441 gcagatatac ggttctacag caactaaaga ggatgcaata gaagtcttta aggattggaa
     7501 tatttcaggt gactctgcat tttcaggtca gacagagcaa ccaccgagct ttgcgtcatt
     7561 tttgaataca atcaaagaca aagttaaagt aactggaagt gataaagaaa cagattaatc
     7621 atgaacatgt atgctcctaa gtaaaaaata cattgaatgt cgcggtaggg atacccgtta
     7681 ccggataccc cccgcacaga tcccggcgtg cgcgatttac gcaccgggct cctgcctcgg
     7741 gtgtctggcg gtgaaccgct ccacaggcca tggatgaaga acccgaaccc ttggtagcca
     7801 tgcggctgcc agtttgtttg ctttcgtcca ggtcgtatca tccttctggc tcctgcgcct
     7861 gagcgcccgg cgccagaggt ttgttacgtg tgtcctgaac ttctgcatgg tggggaagtt
     7921 gcccggtacc gagtgatagt tcaggtatcc ctgaaccact ctcctgagcc attttccctg
     7981 ttcggggatt gagtaatgcc agcgccttcg cagaccgtct ttgatggctt tcagagttgc
     8041 cgtcatccga tcccggcggg tctttcgtat cagcatgaac ctgccgttgc gatctttccc
     8101 gctgatgtgc gtgaacccga ggaagttgaa cgtttctggt ttgccttttc ccctgatggc
     8161 acggttttcg gcagcgaagc ggccgaactc catcagacgg gttttctccg ggtgaaccgt
     8221 gagtccgaac tccctcagtc tgcgctgcat ggctatacgg aagcgccggg catcgtatcg
     8281 tttgtcgaac ccgatgacga tgtcatcggc gtatctgacc attaccacat tgcctgtggc
     8341 atagcgacgt cgccactgat gcgcccacag atcgaagacg tagtggaggt atatgtttgc
     8401 cagcagcggt gagatgaccg caccctgtgg ggtgccttcc tccgttgctc gccattgacc
     8461 ctcctccgac gtcccggctg tgagccactt acgtatgagc ctgattaccc tccggtcgcc
     8521 gatccgatgc tctgtgaacc tgatcagcca ttcgtggctc accctgtcga agaactgact
     8581 gatgtcggca tccagtaccc agtttacgtt agtgcgtacc agccctgtgg ccagtgcgtc
     8641 cagtgcatcg tgctggcttc gcccgggtct gaacccgtat gagaacccca taaagtcgtt
     8701 ttcatagact gcgttcagga ttttcaccag cgcatactgg acgatcttgt cctccagcga
     8761 ggcgatgccg agcgggcgtt gttttccatc cgcttttggg atgtagtgac gcctgccggg
     8821 ctgcgccctg tagctgccct gatgtagcct ccggtgcaga tctgttatgt tgttcttcat
     8881 gtttccggcg tagtccatcc acctgatgcc atccactccg gcggccgctt tcctgctcag
     8941 ggagaggaat gcggcttcca gtgcttcgac tgtcagcagg tggaacaatg ctgtaaaccg
     9001 ttctttcttc cgctgcttcg cagcttcccg cacgcgtgac agcctctgtg acatgctttc
     9061 ccggctctgt gtccggcgca tgtgtggctg ttccgcgttc cccttggccc c
//
