LOCUS       CP094917                2927 bp    DNA     circular BCT 11-APR-2022
DEFINITION  Klebsiella pneumoniae strain 2017HL-01179 plasmid Kpn-WC17-012-05,
            complete sequence.
ACCESSION   CP094917
VERSION     CP094917.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN26849761
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Prussing,C., Southwick,K., Snavely,E., Kidney,A., Randall,L.,
            Sossei,A., Dentinger,L., Shushe,O., Fernandez,R., Haas,W.,
            Lapierre,P., Singh,N., Nazarian,E.J., Musser,K.A. and Mitchell,K.
  TITLE     Comparative analysis of multiplexed PCR and short- and long-read
            whole genome sequencing to investigate a large Klebsiella pneumoniae
            outbreak in New York State
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2020) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 2927)
  AUTHORS   Prussing,C., Southwick,K., Snavely,E., Kidney,A., Randall,L.,
            Sossei,A., Dentinger,L., Shushe,O., Fernandez,R., Haas,W.,
            Lapierre,P., Singh,N., Nazarian,E.J., Musser,K.A. and Mitchell,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2022) Division of Infectious Diseases, Wadsworth
            Center - NYS DOH, 120 New Scotland Ave, Albany, NY 12208, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-NOV-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology             :: ONT MinION and Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/01/2022 13:20:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,953
            CDSs (total)                      :: 5,830
            Genes (coding)                    :: 5,666
            CDSs (with protein)               :: 5,666
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 65 of 164
            Pseudo Genes (incomplete)         :: 109 of 164
            Pseudo Genes (internal stop)      :: 20 of 164
            Pseudo Genes (multiple problems)  :: 26 of 164
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2017HL-01179"
                     /isolation_source="rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="Kpn-WC17-012-05"
                     /geo_loc_name="USA"
                     /collection_date="2017"
     gene            682..873
                     /locus_tag="MQ338_29745"
     CDS             682..873
                     /locus_tag="MQ338_29745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UOQ18014.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            873..1295
                     /locus_tag="MQ338_29750"
     CDS             873..1295
                     /locus_tag="MQ338_29750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UOQ18015.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPSHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1641..1883)
                     /locus_tag="MQ338_29755"
     CDS             complement(1641..1883)
                     /locus_tag="MQ338_29755"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOQ18011.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(2021..2368)
                     /locus_tag="MQ338_29760"
     CDS             complement(2021..2368)
                     /locus_tag="MQ338_29760"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOQ18012.1"
                     /translation="MIMILWWISSFITHMMKVGNVREYGPTKSHLLLMVTTLPAGVGVF
                     IYPIVEVIWGDLSLLKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIA
                     GLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            complement(2383..2760)
                     /locus_tag="MQ338_29765"
     CDS             complement(2383..2760)
                     /locus_tag="MQ338_29765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOQ18013.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLRIESK"
                     /name="hypothetical protein"
     Typing          complement(159..524)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
ORIGIN
        1 aaaccgccca gcaaggcggt tttttcgaag gttcttagag cagcaactct ttgaaccgag
       61 gtaactggct ttcgcagagc acagaaacca aattagttat tacagtgtag ctgtacttca
      121 cctcacactt caacacttct ttgttctgcg aatccgttac cagtggctgc tgccagtggc
      181 gataagtcgt gtctttccgg attggactca agacgatagt taccggataa ggcgcagcag
      241 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
      301 ctgagtgtca ggcgtggaat gagataaacg cggccataac agcggagtga caccggtaaa
      361 ccgaaaggca ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct
      421 ttatagtcct gtcgggtttc gccaccactg atttgagcgt ccgtttctgc gatgcttgtc
      481 aggggggcgg agcctatgga aaaacggcaa cgccgtggcc tttccttttt ttcctccttt
      541 gaccgctcgg ttttctcgag cttttctatg catgctcctg ccgctcgccg cagccgaacg
      601 accggagcgt agcgactgag tgagcgagga agcggaaaag agacaggttt tacactgagc
      661 actgacgctc tgaggccctt tatgacgaaa caacagaaaa cggcgctgaa catggctaaa
      721 ttcatccaga atcagtctct gctcctgctg gagaagctta acgagcttga tcttgatgct
      781 gaggccgacc tttgtgagaa actccacgat gatgcagagc atcttttccg tacactctcc
      841 tcgagactgg attcattaca ggatggtaac tgatggccag agtgtctatt agtgaagctg
      901 cacggcttgt taaggttagt cgtcccacta tttacaagat gattaacagc gggaagttaa
      961 gttatacctc tgttgtaaag cacgggaagg atattaaagt tattgatgta tccgagctta
     1021 ttagggtgtt tggttctctt gatggtgtaa ctgatactgt aaaatatgat gtaaaaagtg
     1081 acgcagaatc tacaggtatt aacagcgttg gtttacatga tttacaacat agaatcgctt
     1141 tacttgaagc tgaaaacgat ggtcttaaag gggctgttaa ggctcgagat gagcatattg
     1201 attcccttcg tcaggctatg cagcttctgg agcacaagca cgaaccttct tcaccatccc
     1261 attccccgtg gtggaaattc tggaaaaaat cctgatcctg ccatgcttga gttttcctct
     1321 ccggccatcc cggagagttt ctgaaccttc ccaaaaatcg ccagatctct gaacgctcct
     1381 caccactaaa aatacccaca ccccacgcaa aattaaaatt ttgcagattc attatttaca
     1441 acaatgggtt aatgaaaaat aaatgtgcgg tgatgtgtta caaagtgcag atccaagtcg
     1501 tggacgactc tatggcgaat cgctgacgaa ttgcccacga ttcatatgtg aatcacgctt
     1561 tattatcata ttgttatgat gaatcgactc ttgattcaat tcatcacttt gggtgttttt
     1621 tgctcatggt gactcttgat tcaattcacg attcgaatcg ccggtgaatc gtgtggtgag
     1681 tcgtccggcg acttgccata acgtgattgc cggccgtcga cgaacccgat tcctgaaggt
     1741 gctgaaaacc ggttttccgg ctgcgccggc aacgcttcac ggccacgcaa taaattgcgt
     1801 ccccgttcac cgttttcgga ggagaagaac gccggaaaca ggacttcccc tggctgaatg
     1861 gtgtgaattt ccgcgctatg cacttgcgcg catactcatg catgccgtaa aaacagagcc
     1921 tgcgcgtttc tggcgggttt tcgggtggtt tgttgcctgt tttaccggtt tcccgtcaga
     1981 aacgccctga gggcctctca ggcggtgctg ataacggatg ttatggtaaa tgttggatag
     2041 caaggactat cagtccggca atgctacata tcattgctaa tggatttacg agcaaaatgc
     2101 cgaatgttgt cgcagataat ccgcgaacaa tgatattggc aagaagtccg ccaacaatga
     2161 gggataaaaa ggtataacca gcaactttca gtaacgacag atctccccat atcacctcta
     2221 ctatcggata gataaaaaca ccaacgccag caggaagcgt agtaaccatc aacagtaaat
     2281 ggcttttagt gggaccgtac tctctgacat ttccaacttt catcatgtgt gtgatgaatg
     2341 acgatatcca ccataggatc atgatcataa ggaaattcat aattatttac tctcgatgcg
     2401 tagatcaggt gctttctgta gatttggtcg tacatactcg ctgtctcttt cgtcaagaca
     2461 acggaagtag agtgttactg tggtatctat ccccattctt gtgttagcca cgttttcgtt
     2521 agtgagcagg aattttttac cgaggctatt acatttctca gaacctgttt taaaggcact
     2581 ttgacgtgct gatgccgcat ctctaaaacc atcagcagtt gctgaaaccg tatatgtatc
     2641 cctacctaac tcaagaacgt tgctggtaga aacacaacca actgtaagca gcgatattga
     2701 taaaatacct aacctataaa tcattgattt tattacccaa gttgggtgat tgtggatcat
     2761 gattatatct gtttttttta ttattaaaat caaggggttc aataattttc ttcaaagaac
     2821 tggtgtcgtg gagtggtgct ggtttccctt tgggggggag agggatggaa taggatcctc
     2881 ttgagatcct ttctgttgcg tcgtaatctc ttgccctgga aacgaaa
//
