LOCUS       CP094916                9294 bp    DNA     circular BCT 11-APR-2022
DEFINITION  Klebsiella pneumoniae strain 2017HL-01179 plasmid Kpn-WC17-012-04,
            complete sequence.
ACCESSION   CP094916
VERSION     CP094916.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN26849761
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Prussing,C., Southwick,K., Snavely,E., Kidney,A., Randall,L.,
            Sossei,A., Dentinger,L., Shushe,O., Fernandez,R., Haas,W.,
            Lapierre,P., Singh,N., Nazarian,E.J., Musser,K.A. and Mitchell,K.
  TITLE     Comparative analysis of multiplexed PCR and short- and long-read
            whole genome sequencing to investigate a large Klebsiella pneumoniae
            outbreak in New York State
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2020) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 9294)
  AUTHORS   Prussing,C., Southwick,K., Snavely,E., Kidney,A., Randall,L.,
            Sossei,A., Dentinger,L., Shushe,O., Fernandez,R., Haas,W.,
            Lapierre,P., Singh,N., Nazarian,E.J., Musser,K.A. and Mitchell,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2022) Division of Infectious Diseases, Wadsworth
            Center - NYS DOH, 120 New Scotland Ave, Albany, NY 12208, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-NOV-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology             :: ONT MinION and Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/01/2022 13:20:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,953
            CDSs (total)                      :: 5,830
            Genes (coding)                    :: 5,666
            CDSs (with protein)               :: 5,666
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 65 of 164
            Pseudo Genes (incomplete)         :: 109 of 164
            Pseudo Genes (internal stop)      :: 20 of 164
            Pseudo Genes (multiple problems)  :: 26 of 164
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2017HL-01179"
                     /isolation_source="rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="Kpn-WC17-012-04"
                     /geo_loc_name="USA"
                     /collection_date="2017"
     gene            271..690
                     /locus_tag="MQ338_29690"
     CDS             271..690
                     /locus_tag="MQ338_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UOQ18003.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            996..1259
                     /locus_tag="MQ338_29695"
     CDS             996..1259
                     /locus_tag="MQ338_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="UOQ18004.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            1249..1548
                     /locus_tag="MQ338_29700"
     CDS             1249..1548
                     /locus_tag="MQ338_29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="UOQ18005.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1610..1987)
                     /locus_tag="MQ338_29705"
     CDS             complement(1610..1987)
                     /locus_tag="MQ338_29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="UOQ18006.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            2056..2235
                     /locus_tag="MQ338_29710"
     CDS             2056..2235
                     /locus_tag="MQ338_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UOQ18007.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2263..2793)
                     /locus_tag="MQ338_29715"
     CDS             complement(2263..2793)
                     /locus_tag="MQ338_29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOQ18008.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2800..3531)
                     /locus_tag="MQ338_29720"
     CDS             complement(2800..3531)
                     /locus_tag="MQ338_29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="UOQ18009.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(3531..5495)
                     /locus_tag="MQ338_29725"
     CDS             complement(3531..5495)
                     /locus_tag="MQ338_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="UOQ18010.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6976..7125)
                     /locus_tag="MQ338_29730"
     CDS             complement(6976..7125)
                     /locus_tag="MQ338_29730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="UOQ18000.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7210..7467)
                     /locus_tag="MQ338_29735"
     CDS             complement(7210..7467)
                     /locus_tag="MQ338_29735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="UOQ18001.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(7477..9162)
                     /locus_tag="MQ338_29740"
     CDS             complement(7477..9162)
                     /locus_tag="MQ338_29740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="UOQ18002.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     BGC             2477..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6295..6873
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2804..3531)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 tgaaaatgat caaaaaaaca gccactcaca ccatgcgcaa tcccctccag atgcaacaaa
       61 aaccagcaca aacggcgccg ttctccggcc tcagtatgtt ttactgaaat cattatatga
      121 gataccagaa agccccctcc gggacaggaa aagaaacggg gggtctggtt cgggttgttc
      181 ggtcactgaa cacggtcaga aactgatgat aagatagcct gcggatatat aactcaatga
      241 gaaaaaagaa ttaaaggtaa ggagacgctc atgagacgtt ccggcagcaa actgtgtctt
      301 atcgccatga taacagcgac agcaactctc actgcctgtg ctccaaaggg cagcgtggaa
      361 cagcataccc ggcattacgt atatgcttca gatgacggtt ttgatcccaa tttttccacc
      421 cagaaagctg acacaacacg aatgatggtg ccttttttcc ggcagttctg ggatatgggc
      481 gcaaaggata aagcgaccgg aaaatcacgg agtgatgtgc agcaacgcat tcagcagttt
      541 cacagtcagg aatttttaaa ctcactccaa ggcacaacac aatttgcggg taccgattac
      601 cgcagcaaag acctcacacc gaaaaaatca aggctactgg ctgacaccat ttcagctgtt
      661 tatctcgatg gctatgaagg cagacagtaa gtggatttac cataacgcgt cattacgcgc
      721 accgctcaga cgggctcagg gcgtttccgg cggctacggg ataaaacagg caacaacccc
      781 cccggaaaac tgccggaaac gcgcccgata aattttatcc taatgcatgc ctatgcacgc
      841 aactgcatgg catatgtttt gacgtaaaat ggatgccgta agcccttttt taccgacttt
      901 ctgcttcatc agcctgactg acacgaccat attctttcag accgccccct gctaaacttc
      961 cttcatattg ccgcattaac ccgggaggct ccgcaatgag caacactaca cactacgaaa
     1021 acgccaactt tcttcgggaa ctggctgaaa gcctgccccg catactgccg gaaggtggcc
     1081 cggacaaagc tgcgctgttg cagcgactgg ctaacgaaga actggcccag gcagagtatg
     1141 aagatcaggt tcgcgccaaa gtgaccgccg cccgtgctga cacgcgtccg ggtatgacca
     1201 cggaacaact ccgccagcgc ctgcatggcc gttatcagga actgcgcgat gccgtatgac
     1261 gtggaatgga agcagggagc catcgaggat gtaacgacgc tgtttgacta tatcgcggaa
     1321 aattcatccc tgtgggatgc gcagaacgtt accgaccggg ttctcgctgc ggcggacaaa
     1381 ctggccgact tcccgcgact ctatgaagtg gactcacgct atggcgagga cgttcgccgg
     1441 atcagtctga tgggacagca tgtcctctat gatgttgatg accagaccag aaaagtccgg
     1501 gttctggccg tggtcggcca gcgccagaat ccccatattg ttctctaata accggtgacg
     1561 gggacgcaat tcattgcgtg gccgtcgccg gttggacaag cacagcgcgt cagcgttttc
     1621 cccacggcca ccaccggcgc ggtgttccgg ccggggtctc tgctgtctcc ggtttgtgtt
     1681 ccagcagtct catggactgc atttcctgcc gtagcccggc cacctcctcc ctgagtgctt
     1741 ttatctccgc gatcaacgcg tcggaggcca ttttttcagt ctcacccgac agtctcaact
     1801 cgtcccgctc gtgagtctca tcctgtctta actcaccgta cacccggata agctcgctgg
     1861 tatccaccac ccggccgcca tcagcttccg tacggtatga tacgcggcct ttcgccatat
     1921 cccgatacag ggaacttcgg gctttacccg tcagttccgt cgcctgtctc accgtcaccc
     1981 acgccattgt ctcaccccgc tatttttgag acagtatacc tgacaggcgc acaccatgcc
     2041 cggaaggaaa gaatcatgac aaaacaggaa aaaacagccc tcaatatggc taaatttatt
     2101 aaggagcagt cgctgctatt gctcgataaa ctgaatgagc tggatatgga taccccggcc
     2161 gacatgtgcg agcggctaca cgaggatgcg gaaacactct ggcaggtgat gcgggataag
     2221 ctcggggagg aataacagca ccggcatggc cggtgctggc atttattttt tgccgaacgg
     2281 ccaccaccgg cgggcaggtt tgtcctccgg cagttcaggt accggcacgt taaaatagcg
     2341 gctgttatat tcccgcatca gctccgcttt cagcttctcg ttcaccgcgc tttccccgtg
     2401 ccgctcactc accacgtcaa tcatcgcgtt aatctgctcc ggtgagcact tcccggcatc
     2461 aaccacctgc acaccgttct cgatggcgaa ccgctccacc ttgcgataga ggtaatcaca
     2521 cagcttcatt cctgtgggtt tgttccgttc ccattgcgga tttttaccag tgaaaaagta
     2581 cgtggctttt tcgtcgctgt tgagccgctc gtcatacatc ccgatggatt tcatcatcgc
     2641 cagatgctgg gcccggtcga caaaacagaa aacatacgcc ccggtctgcc aggcgagcac
     2701 caccatatct tccagatgct cgctggtcgg catgatgcag cgctcgccgt tccggtcctc
     2761 cgcgtcttca ataaatacca tgtacagcgc cataatccct cactccgttg tcataaattt
     2821 aaagcacgca aacaaccgtt cacgatccag ctctgccgtt ttgcgggcct cgccggtctg
     2881 tgggttcacg atttcatcca ccgacaggat tgcccgctgc accctgacct gcgtcgcccg
     2941 gtccgggcag atgtaccaga tttcatcgat gccgttagct ttgcggttga acagataccc
     3001 ggacaccact tcctgatacc ggcgacggct cttgacggtg cgctccacct caaacgccac
     3061 cgttttaccg tccagctccg ccagggcgtc cggcactttc atgcctttaa gactcagacg
     3121 gtgcagaatg cgccagccgg tggcccctct ggcttccata tcaaggcgca gggattgcac
     3181 ggccagccgg tgcggcagat gtgctgccga cacccttgac ggctgaaacg gaataacatc
     3241 cgccagcggt tcgtcctcgt cgaacgagaa cgccacgcca tgcggcgtca gcccccacac
     3301 gctgacctga cggccgtcca ccggatattt ggtccggacc agcagccctt tcgccaccag
     3361 gctgtcgagg aagcggtaga ccggctgcac cgccgcaatc cccagccgcc gcgcaaccag
     3421 ctccgccgtg gaacacagct cttcccgcaa ccagcgcagg gtcagccggg ttttctccgc
     3481 gttccgcgcc tgtcgtttcg cgtggctgac gttgtacagc tccagcgcca tcagtaaatc
     3541 cccgcaaaat catcatccgc tttcggcccg ccgccgtcgg ccgggggccg gaactccgcc
     3601 aggggatcgg ttttcgccag caccgggaaa cgctgcaacg ggatttcccg gcgctcaacc
     3661 cgcagcggac tgctgaacgc catccgggcc acacccagcc cggtcatgac cgcgaacccg
     3721 tccggcaggt gttgcagggt gttcacgtca aacagcggcc gcgcctcctt ctggatgaag
     3781 ctgtccccgc tgatatgctc cacgttcccc gcctcccggc tggcacggcg gcgctccacg
     3841 tccaccctga tttccccggt ctgccccgcc gcccactgcg cggactccag gctggccgcc
     3901 cggtaaaacc agcggatcgg cgtgttatcc agtaccgtgg ttttcacaaa ttcagcgggc
     3961 agatcctgcc cgcactgccc gaaatcgccc agcgactggt gcgcgagcaa cagattgcag
     4021 tcccggctgc gcagcgtccc cagtgcgttc agtacatact tcgacagcag atatttaatt
     4081 tcatcgagca tcacgctgac gtgtggccac tcccggaact cgtcccgcgc aatgatgatt
     4141 tgcgcgcagc gggcaaacag cattttttgc acacggatca ccgcctcgtc gtccatcgag
     4201 ccgataacgt acaggcagcc gccgccgttg ataatccccg ccacatcgat gccactgtcc
     4261 gtctgcagga cgctcaggtc agcgacgttt tccagcctag tgatcaaccc cttcacgtcc
     4321 tttttcaggg cctccggcag cgcgtacgcc gccgccagca cctgctgaat gtttgccccc
     4381 tgcggaaact gctccgcaat cagcttcgcc gctttctgct cgctgatgcg gtagtggtcc
     4441 gccacgtcgc cggtctcgcc caggttaagc ccggcaatca gcaggttttt cagcgcgtac
     4501 tggtcgagat cgcggaacag attcagctgc gccggtttcc ccggccgcag atccagcagg
     4561 gtgaagtccg gacagtgcgc ccggaagacg tgcggcgccc atgcgtcccc tttggggtcg
     4621 aagtacacca ccgcgtcccc gaaatcgcgt acgcagcgaa tgagcgcgtt ggtggccatc
     4681 acgctcttgc cgctcccggg caggccgagg atctgaatgt tggtcttgcg ccaggtatca
     4741 cgcggaacca gcaccggctt gccctgctca tccttaccca gaaacaccgc ctgctcctgc
     4801 cgggcctgct ccgaggaaaa atcggccagc gcattaaccg gtaccggctc cccgaaatgc
     4861 aggtggcgca cgtcggtcat ctctcctgtc cgctggctgc tgcgccggct ctcctgattc
     4921 agtttcgcgt tcaccgcctc gtcagcagcc gcaaaatgac cgtcaagcag caggaacgcc
     4981 agcagccgcg ccaacacaat cccgacagcc agcccgcccg cgcaccaggc aatcccgaac
     5041 aggttagccg gctgcagcag gtaataatct gcgtttgcgt agaggtccgg acgcaggacg
     5101 gtttctcccc agctctcgat aaacacggcg acaaccagcc cccccagaag ggccagaccg
     5161 gccgcgccgg tcagccagta cgcgtagttc cagacatgtc gacgtgccct gaccatccgc
     5221 ccgggcgaat ccagcgaggc cagcaccgaa aatggcgctg gccgtttatt atcgcgctta
     5281 tagcgatcgg catactcccg tcgctcctta tgactcagcc ccacaaaacg catccgtccg
     5341 gcccaaagtt cagccagacc gaaaaacagg ccgataacta ggaaagcccc ggccagaccg
     5401 taatactcct ggtacagcca gctgacccac ggataaatct gccgggccag ctccggaatg
     5461 tgccacttcc aggcaagcga atccgtatta aacatgttct ctctccttca gacgatggtt
     5521 tgccggcgtg atgtgcagcc cccgctgcac aagccgcgga catgccccaa ggcatggcgg
     5581 cggcggtcac gtccgtggtg attgcgttca gctttcacca cggaggccag cctctttccg
     5641 ccccatgcgg aaaaggcgca gccccggagg aacaacgagc acgccccagc gtgtgagagg
     5701 aaaaggcggc accccagtgc ggtcttttcc ggtgttccgg cccccgtcag agttcgccat
     5761 agcgagcggc tgaccacccg caaaatcagg agcagctttg ctgcttcgcc aggtgcgtcc
     5821 agcaccgtgg atttttgtgg gtggtcaggc gtgagcctga cgggggccgg gtgaatttct
     5881 ggcgtggccg gcgtccagcc ggacggagcc agaaagaggg ggagagaccg tgcgtcggtg
     5941 gcgtccagcc agcgtaagca ggggatatcc ccctgaggtc agccacgccc tcgcggggat
     6001 gacctgtatt tctccgtaag gagaaaaaag caacagagat aggttaacgt aaaatgttca
     6061 gcgaaaaacc gatatgattc acctatcctg gtaatttatt atcagggaat ataaagactt
     6121 aatcagggga caatatgcca catcagcgcg tccggtgtcc gtcttgtagg tgccggggaa
     6181 attgcgcctc ctcgctcact cggtcgctac gctccggccg tgagactgcg gcgggcgctg
     6241 cggacacatg caaagttacc cacagattcc gtgaataagc aggggactaa catgtgaggc
     6301 aaaacggcaa ggccacgcag gtggcgtttt tccataggct ccgccctcct gactgagttc
     6361 acataaacag acgctttttc ggtgcatctg tgggagccgg tggctcaacc atgaatcagt
     6421 caggagtggc gaaacccgac aggacttaaa gatcatcacc gtttccgatg aatcgctccc
     6481 tcttgcgctc tcctgttcca accctgccgt ttcccggata cctgttccgc ctttctccct
     6541 tgcgggaagt gtggcgcttt ctcatagctc acactctggt atctcggctc ggtgtaggtc
     6601 gttcgctcca agctgggctg taagcaagaa ccccccgttc agcccgactg ctgcgcctta
     6661 tccggtaact gttcacttga gtccaacccg gaaaagcacg caaaacgcca ctggcagcag
     6721 ccattggtaa ctgggagttc gcagaggatt tgtttagcta aacacgcggt tgctcttgaa
     6781 gtgtgcgcca aagtccggct acactggaag gacagatttg gttgctgtgc tctgcgaaag
     6841 ccagttacca cggttaagca gttccccaac tgacttaacc ttcgatcaaa ccacctcccc
     6901 aggtggtttt ttcgtttaca gggcaaaaga ttacgcgcag aaaaaaagga tctcaagaag
     6961 atcctttgat cttttctact gaaccgcgat ccccgtcagt tcagaggagg acgatggtgc
     7021 caccgttcca ccctgaacat cccggatata gtttgcctga catgcgacca gaagaaagcc
     7081 tgaaaaaatc aatataaaaa gaaaaacaac ctttaccttt ttcatatacc ttccctgtat
     7141 ttaaaaaccc cggtattcat ccctgaacac cggaagtctg tatttcaaat catccttgat
     7201 aacttttgct taccagttac catcccggta aacaaaggat acaaagtaat caaatttact
     7261 aatatcgatt acatttttaa agtgtggctg taatatcgaa acccatgatt tttcaacatc
     7321 aaaccaacca ttattaatat tatcctttaa aggcatcccc agactttcaa tgacagaacc
     7381 atcatcaccg aagtcttttg agtattcacc acctttaaac tcttcggttt tcttatcaaa
     7441 ccaatgaata tgtaatttaa gccccatatt tacctcttaa agatattttt taatgtttcg
     7501 ttttggatcc ggccctttaa cctgcttacc cgtttttgga tcgaatgccc cgaggtgttc
     7561 gccatcactg gcgcggtacc cttcaagctc accgtgctgg gaatcccatt cataaatttt
     7621 acgcttttta tcaccatacc atcgggcccg cttaccacca ccaccttgtt taggggtttt
     7681 aggtttaccc tctttcaact ctcccaaccc cttaatatct tcagtcttgg gatcaggaaa
     7741 ataatcatgg ccgtaatctt tagttccctt acgaggtttt ttcttttcct catttaattt
     7801 attttctgca tccttcgctt ttttctcctt ttgtttccgg ctctccatcg ccccggaaag
     7861 cgcagcctta tccctggata aggattcatt catttcgttt aaagatgtct gggcagcttt
     7921 taatttctca ttctgattct gaatatcctt atcaagatag cgaacctgat cctgccaccc
     7981 ggagccatat tcatgaggcg ggttataagt cacaaaatca tctttatttg cctctgcctc
     8041 ttttaccttt ttatcggctt cctgaacagc agcccttgca ccggggatcg ccttctctgt
     8101 cgatgcaatg cgactgttta aggtcgcaat atttttatct tcagcatcca gctcagcttt
     8161 cgctttatca tactctcttt tcagcccctc ttccgggtga gcagcgtccc atgcctgctg
     8221 gcgacgcttt tcttcctcca gacgctgttt tacctgtgcc ggagtaagaa catccgtcac
     8281 cgacacatag accggtttct gtccgctctc tttcgggaaa cgaataacgg cttcacggga
     8341 gttaccaccg gccgtaaaac cagccggacg tgaatcacct ttttcagcaa taatgccttt
     8401 tggcggggct ttcgcggccg gaacaccttt aggtacgctc acctgcagag acgggagacc
     8461 cggaatcgac acactgaata cccccggacg ttttgtcggt ttcgcatcca ccacaggaac
     8521 actcattggc cggccggtga caaccgcaat atgctgccgc tcgtccttca caatatccgc
     8581 cacacgttta gtgacgctga ccgtcgcctg gtccagcggc agggagctga ccggcgtctc
     8641 cgttaccgtg tctgccggca atgacgtcac gatttttgac atcattttcg gatcatcttt
     8701 tgctatctca gaaggcagca ctccgtaaat cgcaatcccc cacagaccaa acttaaacgg
     8761 acctttcagg gcagccagca catcagcaac agcggccgac aacgcatcgc cggaaacgga
     8821 taaagccagt ccttcagccc ccggagtggc caaggcaggc agaccgaagg ccatcgcggt
     8881 ggccgaagac tgtccgccac cagagctccc gctatgattg ccgctgttac cgccattacc
     8941 accgggttta ctcccgccgt taccaaactc ccccgggtta tagttatgaa tatccccgtt
     9001 aggtgtatgc gtcgtacccc agcccggccc gctgtttgga tccgtaccac cacctgatga
     9061 agggccacct ttacctgatg taccgttcac attcccactg gcctgaccac cattgtgtcc
     9121 cagacctgaa ttacccggac ctcgtccgtc tccgccactc atatactttt cctctttgac
     9181 ttttaaaaca ataaactaaa ataaaatact gtacatataa ccactggttt tatatacagt
     9241 ataaaaatta cgctgccttg tttttcatgt caagtctgta aatttgcgtt tttg
//
