LOCUS       CP094911                2933 bp    DNA     circular BCT 11-APR-2022
DEFINITION  Klebsiella pneumoniae strain 2017HL-00032 plasmid Kpn-WC17-010-05,
            complete sequence.
ACCESSION   CP094911
VERSION     CP094911.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN16823210
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Prussing,C., Southwick,K., Snavely,E., Kidney,A., Randall,L.,
            Sossei,A., Dentinger,L., Shushe,O., Fernandez,R., Haas,W.,
            Lapierre,P., Singh,N., Nazarian,E.J., Musser,K.A. and Mitchell,K.
  TITLE     Comparative analysis of multiplexed PCR and short- and long-read
            whole genome sequencing to investigate a large Klebsiella pneumoniae
            outbreak in New York State
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2020) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 2933)
  AUTHORS   Prussing,C., Southwick,K., Snavely,E., Kidney,A., Randall,L.,
            Sossei,A., Dentinger,L., Shushe,O., Fernandez,R., Haas,W.,
            Lapierre,P., Singh,N., Nazarian,E.J., Musser,K.A. and Mitchell,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2022) Division of Infectious Diseases, Wadsworth
            Center - NYS DOH, 120 New Scotland Ave, Albany, NY 12208, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-NOV-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology             :: ONT MinION and Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/01/2022 13:24:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,634
            CDSs (total)                      :: 5,510
            Genes (coding)                    :: 5,395
            CDSs (with protein)               :: 5,395
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 49 of 115
            Pseudo Genes (incomplete)         :: 71 of 115
            Pseudo Genes (internal stop)      :: 13 of 115
            Pseudo Genes (multiple problems)  :: 17 of 115
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2017HL-00032"
                     /isolation_source="rectum isolate"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="Kpn-WC17-010-05"
                     /geo_loc_name="USA"
                     /collection_date="2017"
     gene            join(2927..2933,1..137)
                     /locus_tag="MBS62_28145"
     CDS             join(2927..2933,1..137)
                     /locus_tag="MBS62_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UOQ12346.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDLSPLAAATGNGFAEQRSV
                     EV"
                     /name="replication initiation protein"
     gene            427..804
                     /locus_tag="MBS62_28150"
     CDS             427..804
                     /locus_tag="MBS62_28150"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOQ12347.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLRIESK"
                     /name="hypothetical protein"
     gene            819..1166
                     /locus_tag="MBS62_28155"
     CDS             819..1166
                     /locus_tag="MBS62_28155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOQ12348.1"
                     /translation="MIMILWWISSFITHMMKVGNVREYGPTKSHLLLMVTTLPAGVGVF
                     IYPIVEVIWGDLSLLKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIA
                     GLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            1304..1546
                     /locus_tag="MBS62_28160"
     CDS             1304..1546
                     /locus_tag="MBS62_28160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOQ12349.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(1898..2320)
                     /locus_tag="MBS62_28165"
     CDS             complement(1898..2320)
                     /locus_tag="MBS62_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UOQ12344.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPSHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2320..2511)
                     /locus_tag="MBS62_28170"
     CDS             complement(2320..2511)
                     /locus_tag="MBS62_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UOQ12345.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          5..284
                     /name="rep_cluster_2374"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.618"
                     /coverage="98.0"
                     /evalue="5.5199999999999996e-67"
     Typing          2670..2933
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.226"
                     /coverage="85.0"
                     /evalue="3.12e-119"
ORIGIN
        1 aaccccccgt tcagtccgac tgctgcgcct tatccggtaa ctatcgtctt gagtccaatc
       61 cggaaagaca cgacttatcg ccactggcag cagccactgg taacggattc gcagaacaaa
      121 gaagtgttga agtgtgaggt gaagtacagc tacactgtaa taactaattt ggtttctgtg
      181 ctctgcgaaa gccagttacc tcggttcaaa gagttgctgc tctaagaacc ttcgaaaaaa
      241 ccgccttgct gggcggtttt ttcgtttcca gggcaagaga ttacgacgca acagaaagga
      301 tctcaagagg atcctattcc atccctctcc cccccaaagg gaaaccagca ccactccacg
      361 acaccagttc tttgaagaaa attattgaac cccttgattt taataataaa aaaaacagat
      421 ataatcatga tccacaatca cccaacttgg gtaataaaat caatgattta taggttaggt
      481 attttatcaa tatcgctgct tacagttggt tgtgtttcta ccagcaacgt tcttgagtta
      541 ggtagggata catatacggt ttcagcaact gctgatggtt ttagagatgc ggcatcagca
      601 cgtcaaagtg cctttaaaac aggttctgag aaatgtaata gcctcggtaa aaaattcctg
      661 ctcactaacg aaaacgtggc taacacaaga atggggatag ataccacagt aacactctac
      721 ttccgttgtc ttgacgaaag agacagcgag tatgtacgac caaatctaca gaaagcacct
      781 gatctacgca tcgagagtaa ataattatga atttccttat gatcatgatc ctatggtgga
      841 tatcgtcatt catcacacac atgatgaaag ttggaaatgt cagagagtac ggtcccacta
      901 aaagccattt actgttgatg gttactacgc ttcctgctgg cgttggtgtt tttatctatc
      961 cgatagtaga ggtgatatgg ggagatctgt cgttactgaa agttgctggt tatacctttt
     1021 tatccctcat tgttggcgga cttcttgcca atatcattgt tcgcggatta tctgcgacaa
     1081 cattcggcat tttgctcgta aatccattag caatgatatg tagcattgcc ggactgatag
     1141 tccttgctat ccaacattta ccataacatc cgttatcagc accgcctgag aggccctcag
     1201 ggcgtttctg acgggaaacc ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa
     1261 cgcgcaggct ctgtttttac ggcatgcatg agtatgcgcg caagtgcata gcgcggaaat
     1321 tcacaccatt cagccagggg aagtcctgtt tccggcgttc ttctcctccg aaaacggtga
     1381 acggggacgc aatttattgc gtggccgtga agcgttgccg gcgcagccgg aaaaccggtt
     1441 ttcagcacct tcaggaatcg ggttcgtcga cggccggcaa tcacgttatg gcaagtcgcc
     1501 ggacgactca ccacacgatt caccggcgat tcgaatcgtg aattgaatca agagtcacca
     1561 tgagcaaaaa acacccaaag tgatgaattg aatcaagagt cgattcatca taacaatatg
     1621 ataataaagc gtgattcaca tatgaatcgt gggcaattcg tcagcgattc gccatagagt
     1681 cgtccacgac ttggatctgc actttgtaac acatcaccgc acatttattt ttcattaacc
     1741 cattgttgta aataatgaat ctgcaaaatt ttaattttgc gtggggtgtg ggtatgggta
     1801 tttttagtgg tgaggagcgt tcagagatct ggcgattttt gggaaggttc agaaactctc
     1861 cgggatggcc ggagaggaaa actcaagcat ggcaggatca ggattttttc cagaatttcc
     1921 accacgggga atgggatggt gaagaaggtt cgtgcttgtg ctccagaagc tgcatagcct
     1981 gacgaaggga atcaatatgc tcatctcgag ccttaacagc ccctttaaga ccatcgtttt
     2041 cagcttcaag taaagcgatt ctatgttgta aatcatgtaa accaacgctg ttaatacctg
     2101 tagattctgc gtcacttttt acatcatatt ttacagtatc agttacacca tcaagagaac
     2161 caaacaccct aataagctcg gatacatcaa taactttaat atccttcccg tgctttacaa
     2221 cagaggtata acttaacttc ccgctgttaa tcatcttgta aatagtggga cgactaacct
     2281 taacaagccg tgcagcttca ctaatagaca ctctggccat cagttaccat cctgtaatga
     2341 atccagtctc gaggagagtg tacggaaaag atgctctgca tcatcgtgga gtttctcaca
     2401 aaggtcggcc tcagcatcaa gatcaagctc gttaagcttc tccagcagga gcagagactg
     2461 attctggatg aatttagcca tgttcagcgc cgttttctgt tgtttcgtca taaagggcct
     2521 cagagcgtca gtgctcagtg taaaacctgt ctcttttccg cttcctcgct cactcagtcg
     2581 ctacgctccg gtcgttcggc tgcggcgagc ggcaggagca tgcatagaaa agctcgagaa
     2641 aaccgagcgg tcaaaggagg aaaaaaagga aaggccacgg cgttgccgtt tttccatagg
     2701 ctccgccccc ctgacaagca tcgcagaaac ggacgctcaa atcagtggtg gcgaaacccg
     2761 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
     2821 cctgcctttc ggtttaccgg tgtcactccg ctgttatggc cgcgtttatc tcattccacg
     2881 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acg
//
