LOCUS       CP094910                9294 bp    DNA     circular BCT 11-APR-2022
DEFINITION  Klebsiella pneumoniae strain 2017HL-00032 plasmid Kpn-WC17-010-04,
            complete sequence.
ACCESSION   CP094910
VERSION     CP094910.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN16823210
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Prussing,C., Southwick,K., Snavely,E., Kidney,A., Randall,L.,
            Sossei,A., Dentinger,L., Shushe,O., Fernandez,R., Haas,W.,
            Lapierre,P., Singh,N., Nazarian,E.J., Musser,K.A. and Mitchell,K.
  TITLE     Comparative analysis of multiplexed PCR and short- and long-read
            whole genome sequencing to investigate a large Klebsiella pneumoniae
            outbreak in New York State
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2020) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 9294)
  AUTHORS   Prussing,C., Southwick,K., Snavely,E., Kidney,A., Randall,L.,
            Sossei,A., Dentinger,L., Shushe,O., Fernandez,R., Haas,W.,
            Lapierre,P., Singh,N., Nazarian,E.J., Musser,K.A. and Mitchell,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2022) Division of Infectious Diseases, Wadsworth
            Center - NYS DOH, 120 New Scotland Ave, Albany, NY 12208, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-NOV-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology             :: ONT MinION and Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/01/2022 13:24:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,634
            CDSs (total)                      :: 5,510
            Genes (coding)                    :: 5,395
            CDSs (with protein)               :: 5,395
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 49 of 115
            Pseudo Genes (incomplete)         :: 71 of 115
            Pseudo Genes (internal stop)      :: 13 of 115
            Pseudo Genes (multiple problems)  :: 17 of 115
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2017HL-00032"
                     /isolation_source="rectum isolate"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="Kpn-WC17-010-04"
                     /geo_loc_name="USA"
                     /collection_date="2017"
     gene            join(9166..9294,1..1557)
                     /locus_tag="MBS62_28090"
     CDS             join(9166..9294,1..1557)
                     /locus_tag="MBS62_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="UOQ12340.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            1567..1824
                     /locus_tag="MBS62_28095"
     CDS             1567..1824
                     /locus_tag="MBS62_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="UOQ12341.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            1909..2058
                     /locus_tag="MBS62_28100"
     CDS             1909..2058
                     /locus_tag="MBS62_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="UOQ12342.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3539..5503
                     /locus_tag="MBS62_28105"
     CDS             3539..5503
                     /locus_tag="MBS62_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="UOQ12343.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            5503..6234
                     /locus_tag="MBS62_28110"
     CDS             5503..6234
                     /locus_tag="MBS62_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="UOQ12333.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            6241..6771
                     /locus_tag="MBS62_28115"
     CDS             6241..6771
                     /locus_tag="MBS62_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOQ12334.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6799..6978)
                     /locus_tag="MBS62_28120"
     CDS             complement(6799..6978)
                     /locus_tag="MBS62_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UOQ12335.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7047..7424
                     /locus_tag="MBS62_28125"
     CDS             7047..7424
                     /locus_tag="MBS62_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="UOQ12336.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(7486..7785)
                     /locus_tag="MBS62_28130"
     CDS             complement(7486..7785)
                     /locus_tag="MBS62_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="UOQ12337.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7775..8038)
                     /locus_tag="MBS62_28135"
     CDS             complement(7775..8038)
                     /locus_tag="MBS62_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="UOQ12338.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(8344..8763)
                     /locus_tag="MBS62_28140"
     CDS             complement(8344..8763)
                     /locus_tag="MBS62_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UOQ12339.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     BGC             1..6557
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2162..2740)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5504..6231
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 agcgggccgg gctggggtac gacgcataca cctaacgggg atattcataa ctataacccg
       61 ggggagtttg gtaacggcgg gagtaaaccc ggtggtaatg gcggtaacag cggcaatcat
      121 agcgggagct ctggtggcgg acagtcttcg gccaccgcga tggccttcgg tctgcctgcc
      181 ttggccactc cgggggctga aggactggct ttatccgttt ccggcgatgc gttgtcggcc
      241 gctgttgctg atgtgctggc tgccctgaaa ggtccgttta agtttggtct gtgggggatt
      301 gcgatttacg gagtgctgcc ttctgagata gcaaaagatg atccgaaaat gatgtcaaaa
      361 atcgtgacgt cattgccggc agacacggta acggagacgc cggtcagctc cctgccgctg
      421 gaccaggcga cggtcagcgt cactaaacgt gtggcggata ttgtgaagga cgagcggcag
      481 catattgcgg ttgtcaccgg ccggccaatg agtgttcctg tggtggatgc gaaaccgaca
      541 aaacgtccgg gggtattcag tgtgtcgatt ccgggtctcc cgtctctgca ggtgagcgta
      601 cctaaaggtg ttccggccgc gaaagccccg ccaaaaggca ttattgctga aaaaggtgat
      661 tcacgtccgg ctggttttac ggccggtggt aactcccgtg aagccgttat tcgtttcccg
      721 aaagagagcg gacagaaacc ggtctatgtg tcggtgacgg atgttcttac tccggcacag
      781 gtaaaacagc gtctggagga agaaaagcgt cgccagcagg catgggacgc tgctcacccg
      841 gaagaggggc tgaaaagaga gtatgataaa gcgaaagctg agctggatgc tgaagataaa
      901 aatattgcga ccttaaacag tcgcattgca tcgacagaga aggcgatccc cggtgcaagg
      961 gctgctgttc aggaagccga taaaaaggta aaagaggcag aggcaaataa agatgatttt
     1021 gtgacttata acccgcctca tgaatatggc tccgggtggc aggatcaggt tcgctatctt
     1081 gataaggata ttcagaatca gaatgagaaa ttaaaagctg cccagacatc tttaaacgaa
     1141 atgaatgaat ccttatccag ggataaggct gcgctttccg gggcgatgga gagccggaaa
     1201 caaaaggaga aaaaagcgaa ggatgcagaa aataaattaa atgaggaaaa gaaaaaacct
     1261 cgtaagggaa ctaaagatta cggccatgat tattttcctg atcccaagac tgaagatatt
     1321 aaggggttgg gagagttgaa agagggtaaa cctaaaaccc ctaaacaagg tggtggtggt
     1381 aagcgggccc gatggtatgg tgataaaaag cgtaaaattt atgaatggga ttcccagcac
     1441 ggtgagcttg aagggtaccg cgccagtgat ggcgaacacc tcggggcatt cgatccaaaa
     1501 acgggtaagc aggttaaagg gccggatcca aaacgaaaca ttaaaaaata tctttaagag
     1561 gtaaatatgg ggcttaaatt acatattcat tggtttgata agaaaaccga agagtttaaa
     1621 ggtggtgaat actcaaaaga cttcggtgat gatggttctg tcattgaaag tctggggatg
     1681 cctttaaagg ataatattaa taatggttgg tttgatgttg aaaaatcatg ggtttcgata
     1741 ttacagccac actttaaaaa tgtaatcgat attagtaaat ttgattactt tgtatccttt
     1801 gtttaccggg atggtaactg gtaagcaaaa gttatcaagg atgatttgaa atacagactt
     1861 ccggtgttca gggatgaata ccggggtttt taaatacagg gaaggtatat gaaaaaggta
     1921 aaggttgttt ttctttttat attgattttt tcaggctttc ttctggtcgc atgtcaggca
     1981 aactatatcc gggatgttca gggtggaacg gtggcaccat cgtcctcctc tgaactgacg
     2041 gggatcgcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2101 taatcttttg ccctgtaaac gaaaaaacca cctggggagg tggtttgatc gaaggttaag
     2161 tcagttgggg aactgcttaa ccgtggtaac tggctttcgc agagcacagc aaccaaatct
     2221 gtccttccag tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt gtttagctaa
     2281 acaaatcctc tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt ttgcgtgctt
     2341 ttccgggttg gactcaagtg aacagttacc ggataaggcg cagcagtcgg gctgaacggg
     2401 gggttcttgc ttacagccca gcttggagcg aacgacctac accgagccga gataccagag
     2461 tgtgagctat gagaaagcgc cacacttccc gcaagggaga aaggcggaac aggtatccgg
     2521 gaaacggcag ggttggaaca ggagagcgca agagggagcg attcatcgga aacggtgatg
     2581 atctttaagt cctgtcgggt ttcgccactc ctgactgatt catggttgag ccaccggctc
     2641 ccacagatgc accgaaaaag cgtctgttta tgtgaactca gtcaggaggg cggagcctat
     2701 ggaaaaacgc cacctgcgtg gccttgccgt tttgcctcac atgttagtcc cctgcttatt
     2761 cacggaatct gtgggtaact ttgcatgtgt ccgcagcgcc cgccgcagtc tcacggccgg
     2821 agcgtagcga ccgagtgagc gaggaggcgc aatttccccg gcacctacaa gacggacacc
     2881 ggacgcgctg atgtggcata ttgtcccctg attaagtctt tatattccct gataataaat
     2941 taccaggata ggtgaatcat atcggttttt cgctgaacat tttacgttaa cctatctctg
     3001 ttgctttttt ctccttacgg agaaatacag gtcatccccg cgagggcgtg gctgacctca
     3061 gggggatatc ccctgcttac gctggctgga cgccaccgac gcacggtctc tccccctctt
     3121 tctggctccg tccggctgga cgccggccac gccagaaatt cacccggccc ccgtcaggct
     3181 cacgcctgac cacccacaaa aatccacggt gctggacgca cctggcgaag cagcaaagct
     3241 gctcctgatt ttgcgggtgg tcagccgctc gctatggcga actctgacgg gggccggaac
     3301 accggaaaag accgcactgg ggtgccgcct tttcctctca cacgctgggg cgtgctcgtt
     3361 gttcctccgg ggctgcgcct tttccgcatg gggcggaaag aggctggcct ccgtggtgaa
     3421 agctgaacgc aatcaccacg gacgtgaccg ccgccgccat gccttggggc atgtccgcgg
     3481 cttgtgcagc gggggctgca catcacgccg gcaaaccatc gtctgaagga gagagaacat
     3541 gtttaatacg gattcgcttg cctggaagtg gcacattccg gagctggccc ggcagattta
     3601 tccgtgggtc agctggctgt accaggagta ttacggtctg gccggggctt tcctagttat
     3661 cggcctgttt ttcggtctgg ctgaactttg ggccggacgg atgcgttttg tggggctgag
     3721 tcataaggag cgacgggagt atgccgatcg ctataagcgc gataataaac ggccagcgcc
     3781 attttcggtg ctggcctcgc tggattcgcc cgggcggatg gtcagggcac gtcgacatgt
     3841 ctggaactac gcgtactggc tgaccggcgc ggccggtctg gcccttctgg gggggctggt
     3901 tgtcgccgtg tttatcgaga gctggggaga aaccgtcctg cgtccggacc tctacgcaaa
     3961 cgcagattat tacctgctgc agccggctaa cctgttcggg attgcctggt gcgcgggcgg
     4021 gctggctgtc gggattgtgt tggcgcggct gctggcgttc ctgctgcttg acggtcattt
     4081 tgcggctgct gacgaggcgg tgaacgcgaa actgaatcag gagagccggc gcagcagcca
     4141 gcggacagga gagatgaccg acgtgcgcca cctgcatttc ggggagccgg taccggttaa
     4201 tgcgctggcc gatttttcct cggagcaggc ccggcaggag caggcggtgt ttctgggtaa
     4261 ggatgagcag ggcaagccgg tgctggttcc gcgtgatacc tggcgcaaga ccaacattca
     4321 gatcctcggc ctgcccggga gcggcaagag cgtgatggcc accaacgcgc tcattcgctg
     4381 cgtacgcgat ttcggggacg cggtggtgta cttcgacccc aaaggggacg catgggcgcc
     4441 gcacgtcttc cgggcgcact gtccggactt caccctgctg gatctgcggc cggggaaacc
     4501 ggcgcagctg aatctgttcc gcgatctcga ccagtacgcg ctgaaaaacc tgctgattgc
     4561 cgggcttaac ctgggcgaga ccggcgacgt ggcggaccac taccgcatca gcgagcagaa
     4621 agcggcgaag ctgattgcgg agcagtttcc gcagggggca aacattcagc aggtgctggc
     4681 ggcggcgtac gcgctgccgg aggccctgaa aaaggacgtg aaggggttga tcactaggct
     4741 ggaaaacgtc gctgacctga gcgtcctgca gacggacagt ggcatcgatg tggcggggat
     4801 tatcaacggc ggcggctgcc tgtacgttat cggctcgatg gacgacgagg cggtgatccg
     4861 tgtgcaaaaa atgctgtttg cccgctgcgc gcaaatcatc attgcgcggg acgagttccg
     4921 ggagtggcca cacgtcagcg tgatgctcga tgaaattaaa tatctgctgt cgaagtatgt
     4981 actgaacgca ctggggacgc tgcgcagccg ggactgcaat ctgttgctcg cgcaccagtc
     5041 gctgggcgat ttcgggcagt gcgggcagga tctgcccgct gaatttgtga aaaccacggt
     5101 actggataac acgccgatcc gctggtttta ccgggcggcc agcctggagt ccgcgcagtg
     5161 ggcggcgggg cagaccgggg aaatcagggt ggacgtggag cgccgccgtg ccagccggga
     5221 ggcggggaac gtggagcata tcagcgggga cagcttcatc cagaaggagg cgcggccgct
     5281 gtttgacgtg aacaccctgc aacacctgcc ggacgggttc gcggtcatga ccgggctggg
     5341 tgtggcccgg atggcgttca gcagtccgct gcgggttgag cgccgggaaa tcccgttgca
     5401 gcgtttcccg gtgctggcga aaaccgatcc cctggcggag ttccggcccc cggccgacgg
     5461 cggcgggccg aaagcggatg atgattttgc ggggatttac tgatggcgct ggagctgtac
     5521 aacgtcagcc acgcgaaacg acaggcgcgg aacgcggaga aaacccggct gaccctgcgc
     5581 tggttgcggg aagagctgtg ttccacggcg gagctggttg cgcggcggct ggggattgcg
     5641 gcggtgcagc cggtctaccg cttcctcgac agcctggtgg cgaaagggct gctggtccgg
     5701 accaaatatc cggtggacgg ccgtcaggtc agcgtgtggg ggctgacgcc gcatggcgtg
     5761 gcgttctcgt tcgacgagga cgaaccgctg gcggatgtta ttccgtttca gccgtcaagg
     5821 gtgtcggcag cacatctgcc gcaccggctg gccgtgcaat ccctgcgcct tgatatggaa
     5881 gccagagggg ccaccggctg gcgcattctg caccgtctga gtcttaaagg catgaaagtg
     5941 ccggacgccc tggcggagct ggacggtaaa acggtggcgt ttgaggtgga gcgcaccgtc
     6001 aagagccgtc gccggtatca ggaagtggtg tccgggtatc tgttcaaccg caaagctaac
     6061 ggcatcgatg aaatctggta catctgcccg gaccgggcga cgcaggtcag ggtgcagcgg
     6121 gcaatcctgt cggtggatga aatcgtgaac ccacagaccg gcgaggcccg caaaacggca
     6181 gagctggatc gtgaacggtt gtttgcgtgc tttaaattta tgacaacgga gtgagggatt
     6241 atggcgctgt acatggtatt tattgaagac gcggaggacc ggaacggcga gcgctgcatc
     6301 atgccgacca gcgagcatct ggaagatatg gtggtgctcg cctggcagac cggggcgtat
     6361 gttttctgtt ttgtcgaccg ggcccagcat ctggcgatga tgaaatccat cgggatgtat
     6421 gacgagcggc tcaacagcga cgaaaaagcc acgtactttt tcactggtaa aaatccgcaa
     6481 tgggaacgga acaaacccac aggaatgaag ctgtgtgatt acctctatcg caaggtggag
     6541 cggttcgcca tcgagaacgg tgtgcaggtg gttgatgccg ggaagtgctc accggagcag
     6601 attaacgcga tgattgacgt ggtgagtgag cggcacgggg aaagcgcggt gaacgagaag
     6661 ctgaaagcgg agctgatgcg ggaatataac agccgctatt ttaacgtgcc ggtacctgaa
     6721 ctgccggagg acaaacctgc ccgccggtgg tggccgttcg gcaaaaaata aatgccagca
     6781 ccggccatgc cggtgctgtt attcctcccc gagcttatcc cgcatcacct gccagagtgt
     6841 ttccgcatcc tcgtgtagcc gctcgcacat gtcggccggg gtatccatat ccagctcatt
     6901 cagtttatcg agcaatagca gcgactgctc cttaataaat ttagccatat tgagggctgt
     6961 tttttcctgt tttgtcatga ttctttcctt ccgggcatgg tgtgcgcctg tcaggtatac
     7021 tgtctcaaaa atagcggggt gagacaatgg cgtgggtgac ggtgagacag gcgacggaac
     7081 tgacgggtaa agcccgaagt tccctgtatc gggatatggc gaaaggccgc gtatcatacc
     7141 gtacggaagc tgatggcggc cgggtggtgg ataccagcga gcttatccgg gtgtacggtg
     7201 agttaagaca ggatgagact cacgagcggg acgagttgag actgtcgggt gagactgaaa
     7261 aaatggcctc cgacgcgttg atcgcggaga taaaagcact cagggaggag gtggccgggc
     7321 tacggcagga aatgcagtcc atgagactgc tggaacacaa accggagaca gcagagaccc
     7381 cggccggaac accgcgccgg tggtggccgt ggggaaaacg ctgacgcgct gtgcttgtcc
     7441 aaccggcgac ggccacgcaa tgaattgcgt ccccgtcacc ggttattaga gaacaatatg
     7501 gggattctgg cgctggccga ccacggccag aacccggact tttctggtct ggtcatcaac
     7561 atcatagagg acatgctgtc ccatcagact gatccggcga acgtcctcgc catagcgtga
     7621 gtccacttca tagagtcgcg ggaagtcggc cagtttgtcc gccgcagcga gaacccggtc
     7681 ggtaacgttc tgcgcatccc acagggatga attttccgcg atatagtcaa acagcgtcgt
     7741 tacatcctcg atggctccct gcttccattc cacgtcatac ggcatcgcgc agttcctgat
     7801 aacggccatg caggcgctgg cggagttgtt ccgtggtcat acccggacgc gtgtcagcac
     7861 gggcggcggt cactttggcg cgaacctgat cttcatactc tgcctgggcc agttcttcgt
     7921 tagccagtcg ctgcaacagc gcagctttgt ccgggccacc ttccggcagt atgcggggca
     7981 ggctttcagc cagttcccga agaaagttgg cgttttcgta gtgtgtagtg ttgctcattg
     8041 cggagcctcc cgggttaatg cggcaatatg aaggaagttt agcagggggc ggtctgaaag
     8101 aatatggtcg tgtcagtcag gctgatgaag cagaaagtcg gtaaaaaagg gcttacggca
     8161 tccattttac gtcaaaacat atgccatgca gttgcgtgca taggcatgca ttaggataaa
     8221 atttatcggg cgcgtttccg gcagttttcc gggggggttg ttgcctgttt tatcccgtag
     8281 ccgccggaaa cgccctgagc ccgtctgagc ggtgcgcgta atgacgcgtt atggtaaatc
     8341 cacttactgt ctgccttcat agccatcgag ataaacagct gaaatggtgt cagccagtag
     8401 ccttgatttt ttcggtgtga ggtctttgct gcggtaatcg gtacccgcaa attgtgttgt
     8461 gccttggagt gagtttaaaa attcctgact gtgaaactgc tgaatgcgtt gctgcacatc
     8521 actccgtgat tttccggtcg ctttatcctt tgcgcccata tcccagaact gccggaaaaa
     8581 aggcaccatc attcgtgttg tgtcagcttt ctgggtggaa aaattgggat caaaaccgtc
     8641 atctgaagca tatacgtaat gccgggtatg ctgttccacg ctgccctttg gagcacaggc
     8701 agtgagagtt gctgtcgctg ttatcatggc gataagacac agtttgctgc cggaacgtct
     8761 catgagcgtc tccttacctt taattctttt ttctcattga gttatatatc cgcaggctat
     8821 cttatcatca gtttctgacc gtgttcagtg accgaacaac ccgaaccaga ccccccgttt
     8881 cttttcctgt cccggagggg gctttctggt atctcatata atgatttcag taaaacatac
     8941 tgaggccgga gaacggcgcc gtttgtgctg gtttttgttg catctggagg ggattgcgca
     9001 tggtgtgagt ggctgttttt ttgatcattt tcacaaaaac gcaaatttac agacttgaca
     9061 tgaaaaacaa ggcagcgtaa tttttatact gtatataaaa ccagtggtta tatgtacagt
     9121 attttatttt agtttattgt tttaaaagtc aaagaggaaa agtatatgag tggcggagac
     9181 ggacgaggtc cgggtaattc aggtctggga cacaatggtg gtcaggccag tgggaatgtg
     9241 aacggtacat caggtaaagg tggcccttca tcaggtggtg gtacggatcc aaac
//
