LOCUS       CP094902                3223 bp    DNA     circular BCT 11-APR-2022
DEFINITION  Klebsiella pneumoniae strain 2017HL-00503 plasmid Kpn-WC17-007-05,
            complete sequence.
ACCESSION   CP094902
VERSION     CP094902.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN16916268
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Prussing,C., Southwick,K., Snavely,E., Kidney,A., Randall,L.,
            Sossei,A., Dentinger,L., Shushe,O., Fernandez,R., Haas,W.,
            Lapierre,P., Singh,N., Nazarian,E.J., Musser,K.A. and Mitchell,K.
  TITLE     Comparative analysis of multiplexed PCR and short- and long-read
            whole genome sequencing to investigate a large Klebsiella pneumoniae
            outbreak in New York State
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2020) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 3223)
  AUTHORS   Prussing,C., Southwick,K., Snavely,E., Kidney,A., Randall,L.,
            Sossei,A., Dentinger,L., Shushe,O., Fernandez,R., Haas,W.,
            Lapierre,P., Singh,N., Nazarian,E.J., Musser,K.A. and Mitchell,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2022) Division of Infectious Diseases, Wadsworth
            Center - NYS DOH, 120 New Scotland Ave, Albany, NY 12208, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-NOV-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology             :: ONT MinION and Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/01/2022 13:33:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,776
            CDSs (total)                      :: 5,651
            Genes (coding)                    :: 5,498
            CDSs (with protein)               :: 5,498
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 64 of 153
            Pseudo Genes (incomplete)         :: 90 of 153
            Pseudo Genes (internal stop)      :: 21 of 153
            Pseudo Genes (multiple problems)  :: 19 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2017HL-00503"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="Kpn-WC17-007-05"
                     /geo_loc_name="USA"
                     /collection_date="2017"
     gene            complement(join(3141..3223,1..133))
                     /locus_tag="MBP03_28855"
     CDS             complement(join(3141..3223,1..133))
                     /locus_tag="MBP03_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UOP97572.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(137..622)
                     /locus_tag="MBP03_28860"
     CDS             complement(137..622)
                     /locus_tag="MBP03_28860"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UOP97573.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(657..1091)
                     /locus_tag="MBP03_28865"
                     /pseudo=""
     CDS             complement(657..1091)
                     /locus_tag="MBP03_28865"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1300..1623)
                     /locus_tag="MBP03_28870"
     CDS             complement(1300..1623)
                     /locus_tag="MBP03_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UOP97574.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2641..2973)
                     /locus_tag="MBP03_28875"
     CDS             complement(2641..2973)
                     /locus_tag="MBP03_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="UOP97571.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(3040..3186)
                     /locus_tag="MBP03_28880"
                     /pseudo=""
     CDS             complement(3040..3186)
                     /locus_tag="MBP03_28880"
                     /inference="COORDINATES: protein motif:HMM:NF016769.2"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     ARG             complement(2642..2973)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(1720..1911)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.821"
                     /coverage="89.0"
                     /evalue="7.28e-73"
     Typing          1891..2374
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(4..1310)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.578"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgttctcccg ttgaataaga ccagacatcg tccgccattc cgcgaaggcg ttctcccact
       61 cgtccagcct tttcgagtag tcctgctgta gctgctctaa tgcgctcagc aactgttttt
      121 ccagctccgt catgtgctat ttccccgcca gtatcatcca gctcgagtcc tctccgaact
      181 gtcccgcttc cgggttcacc ctcacgcagc ggcgtctctg ctcgccgcag gtcgagagct
      241 gtacgccgct gttcctctct gacagcatgg cctgcgtgct cttctgctcc cggatggtcg
      301 tatagttctc gagtatctgc tgcccctgcc accacagaat gcccgcgctc gacgctatca
      361 gcagcgcgga gaccagaacg atggtcagcc acgtctggct gaccatgcgc agcatccctt
      421 tcgtgcgctg gctcatggct gaggacagct tccggtcgtg gtcgaggatg gcggcgctga
      481 ttctcttctc gctttcgctc agttccgctc tgacagactt ctcgtgctcg ctgaatgcgg
      541 ctttcagcat ctcgccggta gtctgctgct gcgctttcga tttctgctct aagtccttcg
      601 ccagcgttaa aagactgttc atagatggct cccttcagtc ggatgtttcg ccccccgtcc
      661 gggtcggcaa tgctgatgct gctttttgtg gtgcgcacca cctcaaaacc tgcgctttcc
      721 agcgcctcag tgacgtcctg acgcgttttc agctccccgg atgaggcaag ggcaagcagg
      781 ccgctcgtaa tcgcctgggc ggcttcctgc ttcgcttccg gcagcgcgga aggcgagacc
      841 agcacccgcc ggttctccgg cgcgttcggg tcatgcagcc ccagcctgcc gttcactatg
      901 gtctgccagg catcgatgcg cggacggtcg gcgcggtcgt aatacggctg tagccgcttg
      961 ccggtcagaa gctccgtgtt cgggatcagg aagttcagct ccagccgccc cttgtcccgg
     1021 tgctcaaccc acagcacgct gtactggtct ttatcgagtc cgggcatcag aacccgctcg
     1081 aaactcgcca tcagcttttc gcgctggccg ggcggtaaat cctgttcggc aaaagacaga
     1141 acgccggaag tgtacttttt ggcgtagggc gaggcgtcga taagctcccg gacttcatcc
     1201 ggcttcccct gcagaacgct ggcgccgtcg cgctggcgat ctttacccag cagataatcg
     1261 accggcccgc cgccgccgcc gcgcccccga ggatgaaact taacgatcat catcagcacc
     1321 cttttccagt acggcatgcc gcaaccgctc gagtccggca tcgatggcca tcagcgcggc
     1381 gacaatctgc acgcggtcgt gtccgcttcc accaccggcg ttaacctggc gggcaatctg
     1441 gttaaggttg ttccccatgc cggcaagctg acgaagcagc gccggcgaga tcgacggaag
     1501 tttgccggcg cgtgcgggct tctcgtccag gcacgtctgc cgcatccagg ccgcgagttg
     1561 cttaccctcg cagcgctcca gcagacgccg gtgctcgtcc tcagtcaccc acatggtgag
     1621 cattttgctg cgtttgtctg ccaatccggc ctcctgataa cgccgggacg gacgggaaat
     1681 cttttcccgg tcggcacggt gttggacaag ccgcagacgc ggcagtggtt ttagcgggtt
     1741 tcggggcgca gccctgaacc agtcacgtag cgctagcgga gtgtatactg gctaaacatg
     1801 ttatgacgga gtggtaattt atgaaagtgc ttcatgtgac aggggaaaaa tgcggctgcc
     1861 gggcgtaagt gcaaaatgca ggtggtgatg aaatgacgcc tcctcgctca ctcggtcgct
     1921 acgctcctgc cgtgagactg cggcgggcgt taccagctca cagattacgg gataagcaaa
     1981 tgccgctcga agcaggaaag gccaagaaaa ggatgcggag ttgccgtttt tccataggct
     2041 ccgcccccct gacgagtatc acgaaaatga cgctcaaatc agcggtggcg aaacccgatg
     2101 cggcttaaag ataccaggcg tttccccctg gtggctccct cgtgctcccc cgcattcctg
     2161 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
     2221 aactcggttc cgggtaggca gttcgctcca ggatggactg tatgcacgaa ccccccgttc
     2281 agtccgacta ccacgcccgt tccggtaact atcaacttga gtccaacccg gaaagacacg
     2341 acaaatcgcc agtggcggta gccattggta actgggttca cggagcagag attgtagaga
     2401 atgcctacag agttcttgaa gtgaaagcct gagtgcggct acactgaaag gacagtttag
     2461 gtatctgcgc tccgtgaaag ccagttacca tcggaacaaa gagttggtag ctctgaattc
     2521 cggcaaacga accaccgttg gtagcggtgg tttttttgtt tacagggaaa aagattacgc
     2581 gcagaaagaa aggatctcaa ggaaatcctt ttgctttggc caatatgctt cggtaaaaag
     2641 ttaatgcgca gaagattttg aaaaaatatt aataaccaaa actccaacgc aaataaggaa
     2701 cattcctgca attgcgggcc aatcaagttt ttgaccgcta accaaccaac cagctaggct
     2761 tattagtaca attcccgcac cagaccaaat tgcgtaagca ataccggtag gtatattttc
     2821 caatgtttgg gaaagcagat aaaaggcagc gcaataactt attgcagttc ctacagacgg
     2881 ccacaggcgt gtaaatcctt ctgaatattt cataaaggtg gtgcctatga cttcagccac
     2941 aatagcccct cccaggaaaa tatatgtatt cataaaaata cctcatgttg aaaaatttaa
     3001 atatctttac ataacaacta attaatagaa cccggcttat catagcgaag gaccatgatg
     3061 ctgccactgc gatcgttcca gctccagcgc tttctggtgc tgcaccgccc gctcctgctg
     3121 ccggtgagtt tctatcgcgt tcagcctttc gacaaccggc gcagctgctc gctcaaactc
     3181 tgcacctgcc tgctcaagac cgtgacgcgc tcgttcagcg ccg
//
