LOCUS       CP094901                3549 bp    DNA     circular BCT 11-APR-2022
DEFINITION  Klebsiella pneumoniae strain 2017HL-00503 plasmid Kpn-WC17-007-04,
            complete sequence.
ACCESSION   CP094901
VERSION     CP094901.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN16916268
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Prussing,C., Southwick,K., Snavely,E., Kidney,A., Randall,L.,
            Sossei,A., Dentinger,L., Shushe,O., Fernandez,R., Haas,W.,
            Lapierre,P., Singh,N., Nazarian,E.J., Musser,K.A. and Mitchell,K.
  TITLE     Comparative analysis of multiplexed PCR and short- and long-read
            whole genome sequencing to investigate a large Klebsiella pneumoniae
            outbreak in New York State
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3549)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2020) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 3549)
  AUTHORS   Prussing,C., Southwick,K., Snavely,E., Kidney,A., Randall,L.,
            Sossei,A., Dentinger,L., Shushe,O., Fernandez,R., Haas,W.,
            Lapierre,P., Singh,N., Nazarian,E.J., Musser,K.A. and Mitchell,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2022) Division of Infectious Diseases, Wadsworth
            Center - NYS DOH, 120 New Scotland Ave, Albany, NY 12208, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-NOV-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology             :: ONT MinION and Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/01/2022 13:33:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,776
            CDSs (total)                      :: 5,651
            Genes (coding)                    :: 5,498
            CDSs (with protein)               :: 5,498
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 64 of 153
            Pseudo Genes (incomplete)         :: 90 of 153
            Pseudo Genes (internal stop)      :: 21 of 153
            Pseudo Genes (multiple problems)  :: 19 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2017HL-00503"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="Kpn-WC17-007-04"
                     /geo_loc_name="USA"
                     /collection_date="2017"
     gene            complement(join(3279..3549,1..152))
                     /locus_tag="MBP03_28825"
     CDS             complement(join(3279..3549,1..152))
                     /locus_tag="MBP03_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UOP97567.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(152..343)
                     /locus_tag="MBP03_28830"
     CDS             complement(152..343)
                     /locus_tag="MBP03_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UOP97568.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1067..1222)
                     /locus_tag="MBP03_28835"
     CDS             complement(1067..1222)
                     /locus_tag="MBP03_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="UOP97569.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(1330..1587)
                     /locus_tag="MBP03_28840"
     CDS             complement(1330..1587)
                     /locus_tag="MBP03_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="UOP97570.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            complement(1589..2191)
                     /locus_tag="MBP03_28845"
                     /pseudo=""
     CDS             complement(1589..2191)
                     /locus_tag="MBP03_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="klebicin B-related nuclease bacteriocin"
                     /name="klebicin B-related nuclease bacteriocin"
     gene            2190..2531
                     /locus_tag="MBP03_28850"
                     /pseudo=""
     CDS             2190..2531
                     /locus_tag="MBP03_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          392..858
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.979"
                     /coverage="92.0"
                     /evalue="2.73e-170"
ORIGIN
        1 ctaataagct ctgaagtatc aataacttta atagccttcc cgtgctttac aacagaggtg
       61 taacttaact tcccactgtt aatcatcttg taaattgtgg gacggctaac cttaacaagc
      121 cgtgccgctt cactaataga cactctggcc atcaattgcc atcctgtaat tcatccagtc
      181 tcgaggacag tgtacggaaa agatgctccg catcatcgtg gagtttctca caaaggtcgg
      241 cctcaacatc gagatcaagc tcgttaagct tctccagcag gagcagagac tgattctgga
      301 tgaatttagc catgttcagc gctgttttct gttgtttcgt cataaagggc ctcagagcgt
      361 cagtgctcaa tgtcagacag atctcttttc cgcttcctcg ctcactcagt cgctacgctc
      421 ctgccgttcg gctgcggcga gcggtcgcaa cgcgcacata acgacataga gcagacagct
      481 cggagaggga gaaggcagca cagcggccgt ttttccatag gctccgcccc cctgacaagc
      541 atcacagaat ttgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
      601 aggcgtttcc ccctgatggc tccctcgtgc gctctcctgt tcctgccatt cggtttaccg
      661 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
      721 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgactgctgc
      781 gtcttatccg gtaactatca tcttgagtcc aacccggaaa gacacgacaa agcgccactg
      841 gcagcagcca ctggtaacgg atttagagga gttggtcttg aagtcatgcg cctgttaagg
      901 ctaaactgaa agaacaagtt ttggtgactg cgctcctcta agccagttac cttggttcaa
      961 tcacctgcaa gtgtttgaac cttcggaaaa accgccctgc aaggcggttt tttcgtttac
     1021 agagcaagag attacgacga tccaaatggg atctcaagaa gatcctttac ggattgccgt
     1081 tcgtaccgct atcaagcgtg actccggtca ccgtcgatga aggtgaaaca gccccacctc
     1141 cggtgtctcg aacattattg acctgacatg ctgccagaac cacagaaacg gtaacaaccc
     1201 ctaggaaaag taattttttc atatatcacc ttttattttt attattctag gcaaatggat
     1261 atctagtaat tattcttcaa atccagagtt acaaatgagc gggctcataa cttgaacccg
     1321 caaattcaat cacgcttttt taaaacctgg cttaccgtta gcagctcgcc attcctttac
     1381 ggttttaaca attcctttcg ccgaagaatc ttcgcctacc ttaggcttga agatgaggtt
     1441 ccaaccagca ggatgctctg ttagttgggc gaacgaataa atagcatcat cgttctcttt
     1501 gtcagttgga aaatcagcag cgattatttt actaacaaaa gcagtaaatt cttcttccgt
     1561 aaaatctgaa attttattac cagccatttt attttccttt atgtgtctca atatgatttt
     1621 taggtgttag cgccttaatg ttatcgacat ccataacagc ccctccttga gatatttctt
     1681 tttcgtggtg taattcaatt ttaacacgtt tccctactga ttctaaaaag tccgcagtag
     1741 gtgcccttcc agctttcatc tgattaatat cagcttcact aaattgcttg ctcagtgcag
     1801 aatcgtcagc aaccgctttc cagaacgccc gacggaaaga atcaaaacta ccgaaagtct
     1861 tcccgcgtaa tttatccgca atctgggaag gaacaggagc accatccccg gtactggccc
     1921 cccccatcca gttattgcca acgttctgcc ctttgccgct cactgtaccg ggcatgttac
     1981 gagggctacg gtacatcaca tacagcggtt ttaggccgga ttccggagga aacaccagaa
     2041 taatatcgtc aaaatcgagg tcatccgcta ccggcgtcac ggttttatct gtagcctgtg
     2101 gcgccgaaat ctgatcaccg gtatgcagaa tacgctccgg tagtggtacc ggcccgctta
     2161 gcgtcagtgg tccgtttgca ccaggtgcgt caagtgcact agatttcacc aggatggcgc
     2221 ttaatcagga aaaatacctc gatgacttta catggaaaga actgaacgac gttatcaact
     2281 tcatacgcaa agcagaaggt agtgcatctg accacagcta tgcactccag ttactggaga
     2341 ttaatttcaa ggcaaaccca ctggatctca tctatcaccc tgactgctgg ttcaatgatg
     2401 aagcattgtt tcatgcccgg ctgactaccg aagaaattgg tggctactta atgaaaaaat
     2461 caggtcgatg gctcaacgat gcaccggata tccaactggt gtatgccatc ccccaggatg
     2521 tttatgatta accaagtcac catttaccat aatcccttaa ttgtacgcac cgctaaaacg
     2581 gcctcaggac gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
     2641 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcgcaa gtgcatagcg
     2701 cggaaattca cgccattcag ccagggtaag tcctgtttcc ggcgttcttc tgatccgaaa
     2761 acggtgaacg gggacgcaat ttattgcgtg gccgtgaggc gttgccggcg cagccggaaa
     2821 accggttttc agcacgttca ggaatcgggt tcgtcgacgg ccggcaatca cgttatggca
     2881 agtcgccgga cgactcacca cacgattcac cggcgattcg aatcgtgaat tgaatcaaga
     2941 gtcaccatga acaaaaaacg cccaaattga tgaattgaat caagagtcga ttcatcataa
     3001 caacatgata ataaagcgtg attcacatat gaatcgtggg caattcgtaa acgattcgcc
     3061 atagagtcgt ccacgacttg gatctgcact ttgtaacaca tcactgcaca ttaatttttc
     3121 attaaccaat tgttttaaat aaggaatctg caaaatttta attttgcgta gtgtgtgggt
     3181 atttttagtc gtgaggagcg ttcagagatc tggcgatttt tgggaaggtt caggaactcc
     3241 ctgggatggc cggagaggaa aactcaagca tggcaggatc aggatttttt ccagaatttc
     3301 caccacgggg aatggggtgg tgaagaaggc tcgcgcttgt gctccagaag ctgcatagcc
     3361 tgacgaaggg aatcaatatg ctcatctcga gccttaacag cccctttaag accatcgttt
     3421 tcagcttcaa gtagagcgat tctatgttgt aaatcatgta aaccaacgct gttaatactt
     3481 gtagattctg cgtcactttt tacatcatat tttacagtat cgatgacacc atcaagagaa
     3541 ccaaaaact
//
