LOCUS       CP093456                6673 bp    DNA     circular BCT 22-MAY-2023
DEFINITION  Klebsiella pneumoniae strain VA833 plasmid pVA833-7, complete
            sequence.
ACCESSION   CP093456
VERSION     CP093456.1
DBLINK      BioProject: PRJNA815754
            BioSample: SAMN26642723
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Complete genome sequencing and analysis of carbapenem-resistant
            Klebsiella pneumoniae isolated in Chile
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2022) Biology, Grupo de Microbiologia Integrativa,
            Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Nunoa,
            Santiago, RM 780000, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Flye v. 2.9-b1768; Tricycler v. 0.5.0; Raven v.
            1.5.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 220X
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 03/16/2022 16:30:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,990
            CDSs (total)                      :: 5,866
            Genes (coding)                    :: 5,539
            CDSs (with protein)               :: 5,539
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 327
            CDSs (without protein)            :: 327
            Pseudo Genes (ambiguous residues) :: 0 of 327
            Pseudo Genes (frameshifted)       :: 219 of 327
            Pseudo Genes (incomplete)         :: 109 of 327
            Pseudo Genes (internal stop)      :: 20 of 327
            Pseudo Genes (multiple problems)  :: 20 of 327
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="VA833"
                     /isolation_source="Isolated from blood of an 54 years old
                     female"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pVA833-7"
                     /geo_loc_name="Chile: Metropolitan Region"
                     /collection_date="2019-04-23"
                     /collected_by="Instituto de Salud Pblica de Chile"
     gene            complement(join(6669..6673,1..970))
                     /locus_tag="MO408_29370"
     CDS             complement(join(6669..6673,1..970))
                     /locus_tag="MO408_29370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHQ94329.1"
                     /translation="MLKKSSVALALSLALSGYASAAEAPIVDSKTDLGTLRPGNGGYSY
                     ASGVSADGTVVVGESRTDSGDYNAFVWREGDTKMTGLGTLKADNSGYSSASGVSADGTV
                     VVGYSDTDEGDTHATVWKVKYPVPPVEPPVVTIVDATNSSKAMADTGRRGFKVLDLYQS
                     SLNSLSQSRCQLGQSDYCVGVFSQYDTVSSNHRLATGLFGAIRLPAENWTVGGAMNFAN
                     GTQLIDNYDTRGSNQPGIGVFTRYQENRDNTGLSAELSGVVIRCSRENELVRYSSNSCP
                     ECSIRWHVVDAVGQDIRPLEKTFHNACQESYWLKVNRQTHLPV"
                     /name="hypothetical protein"
     gene            complement(1365..1790)
                     /locus_tag="MO408_29375"
     CDS             complement(1365..1790)
                     /locus_tag="MO408_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WHQ94330.1"
                     /translation="MALVSVTEAARLTGKNRTTIHRYIKQGKLSQISDATNTKKVDTSE
                     LIRVFGSLTATHATLSQSDAMQHDATPKTDQSVALLQQKIALLEQLLENKNKELQRQDE
                     HIDSLKQAMLLIESKLPTTPEPVTPVPAKKSWQFWKK"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1798..2754)
                     /locus_tag="MO408_29380"
     CDS             complement(1798..2754)
                     /locus_tag="MO408_29380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WHQ94331.1"
                     /translation="MSELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTIENPTRDE
                     LTVKFSCSEYARTMGLSYENAWGRLNSATSDLFKRSVELIYPTGAVSKRIFNWTEYAEF
                     NREEQTVTLVFSSYIQPLLFHLKKFIKYNLEHVKAFENKYSMRIYEWLLKELSQRKTHR
                     GNIEISIKEFKFMLMLEKNYPLYAELNRWILKPVTNDLNTYSNMKLTIEKRGRPADTLI
                     FQVELDKQIDLVTELEKDPASKKEDKTIRLTPENRLHEGLKTTLHDALTAKIQLTGFEA
                     KFLSDMQSKYDLNGSFSWLTQKQRATLEKILAKYGRI"
                     /name="replication initiation protein"
     gene            complement(3350..3805)
                     /locus_tag="MO408_29385"
     CDS             complement(3350..3805)
                     /locus_tag="MO408_29385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WHQ94326.1"
                     /translation="MKNEKQAKWLTDRVQYIRGLKAPNEQQRLLVILHDKTDKTAQEIK
                     TLSLLIQAERAAEKAQEARTKVMNLIHAEKRAEAKAARKARDHALYQSAGLLIMAGLVD
                     SQTGKPVDDPAKLLGALASLHELSRDNPRWEEWKLKGQVLLNQKKED"
                     /name="conjugal transfer protein TraD"
     gene            3971..5086
                     /gene="mobA"
                     /locus_tag="MO408_29390"
     CDS             3971..5086
                     /gene="mobA"
                     /locus_tag="MO408_29390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WHQ94327.1"
                     /translation="MASYHLSVKTGGKGKAASHADYIAREGKYDREKDSDLEHKESGNM
                     PAWAAHKPAEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFIRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKEPERGGAKKVRFG
                     ETPTERREYLIAQRERWATLQNNHLERYQHPDRVDARSLKAQGIDREPERHLGAGQVQR
                     FDTDQLQAILERRDAERQVQQCCDERDSVIDVTTSLREALIERDTLTPKQAQQPEPEQD
                     AVSGRVFDFEKEPDKLNELVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMVRQQERTA
                     LAEKQRQQEKERQRIAEQKQQKPDKGWSFSR"
                     /name="mobA"
     gene            5099..5683
                     /locus_tag="MO408_29395"
     CDS             5099..5683
                     /locus_tag="MO408_29395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="WHQ94328.1"
                     /translation="MSDEIKQIAALIGHQQAQEKRVAALIDEFQAESAKLSRQTAQLAQ
                     VIHELDSASGKMTDTIRQSVNAALTQVEKELKQTGLAQQKPAVNALEQVVTAANASVSA
                     MRREMSRYTWKSAIYIVLTMLAVMGSCLWGMVCFIDSGYDRIAKMERMEAVWQKKAPLA
                     DISTCDGKPCVKVDTSQSYGDKKDTYLIIKQ"
                     /name="tRNA modification GTPase"
     gene            5773..5922
                     /gene="pspF"
                     /locus_tag="MO408_29400"
                     /pseudo=""
     CDS             5773..5922
                     /gene="pspF"
                     /locus_tag="MO408_29400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /note="transcriptional activator for pspABCE which are
                     induced in response to phage proteins, membrane altering
                     stresses, and impaired proton expor; incomplete; partial on
                     complete genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /name="pspF"
     gene            6016..6660
                     /locus_tag="MO408_29405"
     CDS             6016..6660
                     /locus_tag="MO408_29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WHQ94332.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     ARG             6017..6657
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(1799..2754)
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.97"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3972..5083
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.175"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccgggagatg cgtttgacga tttactttta accagtacga ctcctggcaa gcattgtgaa
       61 acgttttctc cagtggacga atatcctgac ctaccgcatc aacgacatgc catctaatgc
      121 tgcattctgg acaggaattt gatgaatatc taaccaattc attttcgcga gaacaacgaa
      181 taaccactcc cgataactcg gcactcagac ccgtattatc gcggttttcc tgataacggg
      241 tgaatacccc gatgcccggc tgattgctgc cgcgcgtgtc gtagttatca ataagctgag
      301 tgccgttggc gaagttcatc gccccgccca ccgtccagtt ttcggccggt aaacggattg
      361 ccccgaacag accggttgcc agacggtgat tactgctgac ggtatcatac tggctgaaaa
      421 ccccgacgca ataatcactc tggcctaact gacaacggct ctgacttaag ctgtttaagc
      481 tgctctggta caggtctaac actttaaaac cgcgacgacc ggtatccgcc atcgctttgg
      541 atgaattggt tgcatcgact atcgttacta ccggcggctc taccggcggc accgggtatt
      601 tcactttcca cacggtggca tgggtatcac cttcatcagt atctgagtac ccgactacta
      661 cagtcccgtc cgccgagacc ccactggcag aggaataccc gctgttgtcg gctttaaggg
      721 tgccgagacc ggtcattttg gtgtctccct cacgccacac aaaggcatta tagtcgccgc
      781 tgtcagtcct ggactctccc actactactg tgccgtccgc cgagacccca ctggcatagg
      841 aataccctcc attccccggg cgtaaagttc ccaggtcagt tttactgtcc acaatggggg
      901 cttctgccgc ggaggcatac ccacttaatg ccaggcttaa tgccagggct acactgcttt
      961 tttttaacat attgttatcg ctccaaaaag aagtcactta cgtgacaaaa taaaattttt
     1021 tatttgtacc gctgcggtgt gggggcagcg atggtccggg tagaggtcat cctggttggc
     1081 tatagtggcg gggggtttaa aataaaattt taaagtattt tgatataaac aaacaaatat
     1141 ttattttttt tttttttgtt ataaattaaa tgattaccgt tctgctattg ctcagattta
     1201 acataagggt gcttaagcgc acttccgttt aggctttgct cctgaatttt ttctcaaccg
     1261 cgcaaaatgc cctgttttct ccctgtaaaa agcccgattt ttatgttgcc aatttgttat
     1321 caaaatgacg atttcggttt cagggtgaaa atgccgccaa ccggctattt tttccagaat
     1381 tgccacgatt ttttggctgg tactggtgta acgggttcag gtgttgtcgg cagcttggat
     1441 tcgattaaca gcattgcttg tttcaggctg tctatatgct cgtcctgacg ttgtaattct
     1501 ttatttttgt tttcaagcag ttgctctaaa agtgcaattt tttgttgtaa cagtgcaacg
     1561 gattgatctg ttttaggtgt tgcatcgtgc tgcattgcat cgctttgcga cagtgttgca
     1621 tgtgtcgccg ttagtgagcc gaatacacga ataagctctg atgtatctac tttttttgtg
     1681 tttgttgcat cagaaatttg cgacagtttt ccttgcttaa tatagcgatg tattgttgtt
     1741 ctgttttttc cagttagtct ggctgcctct gtaactgata ccagtgccat agatacctca
     1801 gatccgtccg tattttgcca atattttctc tagcgtggct cgttgttttt gagtcagcca
     1861 tgagaacgag ccgtttagat catatttgct ctgcatgtca ctcagaaatt tagcttcaaa
     1921 gccggtaagc tgaatttttg cagttaaagc atcatgcaat gttgttttta gcccctcatg
     1981 aagacgattt tcaggtgtta aacggattgt cttatcttct ttttttgatg ctggatcttt
     2041 ttctagttca gtcacaaggt caatttgctt atccagttca acttgaaaga tcagtgtgtc
     2101 agcaggacga ccgcgttttt caatagtcaa cttcatattg ctgtaagtgt ttaagtcatt
     2161 tgtaactggt tttaggatcc agcgattcaa ttctgcatat aaagggtagt ttttttcaag
     2221 catgagcata aacttaaatt ccttgatact tatctcaatg tttcccctat gcgtttttct
     2281 ttgtgaaagc tcctttagta accattcata tattcgcatt gagtatttat tttcaaaggc
     2341 tttaacatgc tcaaggttgt atttaatgaa tttttttaaa tgaaataaaa gtggttgaat
     2401 atatgagcta aatactaatg ttacagtttg ttcttctcta ttgaactcag cgtattccgt
     2461 ccaattgaaa attcgtttag aaactgctcc tgtaggataa attaattcaa cagagcgttt
     2521 aaataaatca ctagttgcac tgttcaatct tccccaagca ttttcataac ttaagcccat
     2581 agtgcgcgca tattcagaac atgagaattt aactgttaat tcatcccttg ttgggttttc
     2641 aattgtaggg ttcaactttg caacgcaaaa cagaattagc ttggtttcat gttcagttag
     2701 atcataacgg cttactgcta attcatttgc tttaaacaca actaattcag acatgccaat
     2761 caccattatg tagtaattta aatcgccaca taaatcataa taatgtagca attaaaatca
     2821 actacatttt ttatgtttga ttgtgaataa cctaattttt cctacatctt tcccactgct
     2881 agcctaattt ttcctacatc cagcctaatt ttccctacat ttaatgattt tttttttatt
     2941 aaaaaacaat gatttaaaat cactagaata aagaaagaat aataaagata aaaaaagaat
     3001 agatcccagc cctgtggata actcagaaaa ctgccgtcga tttgccgtag ttaaaaggat
     3061 gtcgcaaaca aggttttctc cttttcaaaa caaacctaaa acacctcagt agctccctcg
     3121 ctttgcccgg gcaactcgct gaccctttct tttgtctccg accatccagc cactgcgccg
     3181 ctgttttttc cgtgtcattg agttcgcttc gctcacggct caatcaggaa agtggctgta
     3241 tgcgcttctg tgccacgctt tcagtgttag gcgatgaaat ccattaccga gcctgtaaaa
     3301 cctcgccacg gacttcctgt ggcgttttag agggtattaa caccctgttc taatcttcct
     3361 ttttctgatt gagtaatacc tgccccttca atttccattc ttcccacctg ggattatcac
     3421 gggataattc atgcaagctg gctaatgcgc ccagtagttt ggcggggtca tccaccggtt
     3481 tacccgtctg actgtccact aaccctgcca taatcagtaa tcccgccgat tgataaaggg
     3541 catggtcacg cgctttacgg gcggctttgg cttctgctcg tttctcagcg tgtatcaggt
     3601 tcatcacttt cgtgcgggct tcctgtgctt tttctgctgc acgctctgcc tgaattaaca
     3661 atgagagcgt ctttatttcc tgagcggttt tatccgtttt atcgtgcaat atcaccaata
     3721 aacgttgttg ttcattcggt gcttttaaac cacggatata ctgcactctg tctgtgagcc
     3781 acttagcctg tttttcgttt ttcatcttta ccactcatgc tgtttttcga atggaataat
     3841 tatgctcatt attggcaact caggtcaaat tccactacaa tccctgtcat cctttttatt
     3901 gacagtgcgc acttagacgt ttcttcacta tgttcagaaa cggtgcgcgc ctgcggcggg
     3961 cgaaaacact atggcctctt atcacctcag cgtcaaaacc ggcgggaaag gcaaagccgc
     4021 ttcccatgcc gattatattg cgcgtgaggg aaaatatgac cgtgaaaaag actccgacct
     4081 tgagcataag gagtccggca atatgcccgc atgggcagcg cataaaccgg ctgagttctg
     4141 gaaagccgct gacacgtttg aacgtgccaa cggctgcacc taccgcgaga ttgaaatcgc
     4201 cctgccacga gagctgaaac ctgagcagcg gcttgagctg gttcgcgatt ttatccggca
     4261 ggaaattggc gaccgtcatg cgtatcagtt tgctatccat aacccgaaag cagcgatagc
     4321 cggtggcgaa cagccgcatg cgcatatcat gttctctgaa cggatgaatg acggcattga
     4381 ccgtgatccg cagcagtatt ttaaacgggc gaacagtaaa gagcctgagc gtggcggcgc
     4441 gaaaaaggtg cggttcggcg aaacgccgac cgaacgcagg gaatacctta tcgcgcagcg
     4501 ggaacgctgg gcaacattac aaaataacca tcttgagcgt tatcagcatc ctgaccgtgt
     4561 ggatgcgcgt tccctgaaag cacagggtat cgatcgtgag ccggagcggc atctgggcgc
     4621 gggacaggtt caacgcttcg atactgacca gttacaggcc attcttgaac gcagggatgc
     4681 cgaaagacag gttcagcagt gctgtgatga acgtgacagc gtgattgacg tgaccacttc
     4741 gttacgggaa gcccttattg agcgggacac tctgacgcca aaacaggcgc agcagccgga
     4801 gcctgaacag gacgccgttt ccggcagggt gttcgatttt gaaaaagagc ctgacaagct
     4861 gaatgaactg gtcagcgatg ccatgaagga catacaagaa gaaatcgacc ttcagtcact
     4921 tgttaacgat gcgatggcag agtttcaggg catacatcag gagatggtca ggcagcagga
     4981 acgcaccgcg cttgctgaaa agcaaagaca gcaggagaaa gaacggcagc gtattgccga
     5041 acagaaacaa caaaaaccag acaaaggctg gtcattttca cgatagacag gattttacat
     5101 gagcgacgag attaaacaga ttgctgccct tatcgggcat cagcaggcac aggaaaaacg
     5161 cgtagccgcg ctgattgatg aatttcaggc ggaatcagcc aaattaagcc gccagacagc
     5221 acaattggcg caggtaattc atgaactgga cagtgcctcc ggtaagatga ccgacacgat
     5281 caggcaatcg gtgaatgccg ctttaacgca ggttgaaaaa gaactcaaac aaacgggatt
     5341 ggcgcagcag aaacccgccg tcaatgcgct ggaacaggtg gttacagcgg ctaatgcgtc
     5401 tgtgagtgca atgcgacgtg agatgtcgcg ctatacgtgg aagtctgcaa tttatattgt
     5461 tctgacgatg cttgcggtca tggggagctg tctttggggg atggtctgtt ttatcgacag
     5521 tggctatgac cgaattgcaa aaatggagcg catggaagcg gtctggcaga aaaaagcccc
     5581 gctggctgat atctccacgt gtgatggaaa accttgcgtg aaagtggata ccagtcagtc
     5641 atatggcgat aaaaaagaca cgtatctgat tatcaaacaa taaaaaacgg cgctgaatat
     5701 gcctcaaatg ccaccggcga tttccagccg agtgttgaat gtcttcggcg tggattataa
     5761 aagccattta cgctggatct gcgtgaattc cagcagcagc aagagaaaga ctttctacaa
     5821 accagcttac agcaggctaa atttaaccag aaaaaagccg ctgaattact gggtctgact
     5881 tatcatcagc tacgtgcctt gctgaagaaa caccagatct aatacgcatt tgcaggtgtc
     5941 atactggcgg gtttgacgca taacgtcata aggtttacca tggcgtcatt actgtataaa
     6001 aacacaggca tagatatgac tctggcatta gttggcgaaa aaattgacag aaatcgcttc
     6061 accggtgaga aagttgaaaa tagtacattt tttaactgcg atttttcagg tgccgacctg
     6121 agcggcactg aatttatcgg ctgccagttc tatgatcgcg aaagtcagaa agggtgcaat
     6181 tttagtcgcg caatgctgaa agatgccatt ttcaaaagct gtgatttatc aatggcagat
     6241 ttccgcaacg tcagtgcctt gggcattgaa attcgccact gccgcgcaca aggcgcagat
     6301 ttccgcggtg caagctttat gaatatgatc accacgcgca cctggttttg cagcgcatat
     6361 atcactaata ctaatctaag ctacgccaat ttttcgaaag tcgtgttgga aaagtgtgag
     6421 ctgtgggaaa accgctggat ggggactcag gtactgggtg cgacgttcag tggttcagat
     6481 ctctccggcg gcgagttttc gactttcgac tggcgagcag caaacttcac acattgcgat
     6541 ctgaccaatt cggagttagg tgacttagat attcggggtg ttgatttaca aggcgttaag
     6601 ttagacagct accaggcatc gttgctcatg gagcggcttg gcatcgctgt gattggttag
     6661 ttcagcgttt aca
//
