LOCUS       CP093136                5466 bp    DNA     circular BCT 05-MAY-2022
DEFINITION  Salmonella enterica strain PNUSAS127695 plasmid pPNUSAS127695_1,
            complete sequence.
ACCESSION   CP093136
VERSION     CP093136.1
DBLINK      BioProject: PRJNA230403
            BioSample: SAMN13795856
KEYWORDS    .
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Webb,H.E., Kim,J.Y., Tagg,K.A., de la Cruz,F., Penil,A., Tolar,B.,
            Ellison,Z., Schwensohn,C., Brandenburg,J., Nichols,M. and
            Folster,J.P.
  TITLE     Twenty-one complete Salmonella enterica serotype Hadar genomes
            collected from patients in the United States
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Tarr,C.L., Trees,E., Katz,L.S., Carleton-Romer,H.A., Stroika,S.,
            Kucerova,Z., Roache,K.F., Sabol,A.L., Besser,J. and Gerner-Smidt,P.
  CONSRTM   PulseNet: The National Subtyping Network for Foodborne Disease
            Surveillance
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2020) Enteric Diseases Laboratory Branch, Centers
            for Disease Control and Prevention, 1600 Clifton Rd., MS C03,
            Atlanta, GA 30333, USA
REFERENCE   3  (bases 1 to 5466)
  AUTHORS   Webb,H.E., Kim,J.Y., Tagg,K.A., de la Cruz,F., Penil,A., Tolar,B.,
            Ellison,Z., Schwensohn,C., Brandenburg,J., Nichols,M. and
            Folster,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2022) EDLB, Centers for Disease Control and
            Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: PNUSAS127695_consensus
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2022 09:14:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,698
            CDSs (total)                      :: 4,570
            Genes (coding)                    :: 4,449
            CDSs (with protein)               :: 4,449
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 19
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 48 of 121
            Pseudo Genes (incomplete)         :: 87 of 121
            Pseudo Genes (internal stop)      :: 24 of 121
            Pseudo Genes (multiple problems)  :: 34 of 121
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="PNUSAS127695"
                     /db_xref="taxon:28901"
                     /plasmid="pPNUSAS127695_1"
                     /geo_loc_name="USA"
                     /collected_by="CDC"
     gene            join(4896..5466,1..239)
                     /locus_tag="GUD44_23390"
     CDS             join(4896..5466,1..239)
                     /locus_tag="GUD44_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UPX62694.1"
                     /translation="MSKDKKGRSKNRYHQLVEKIFFDKYVDGDLEVLFERLEINSAAKD
                     LGIDLPSNVGDVIYSIRFRTPMPESILLTQPEGKEWIIELAGRSKYRFRLVTISRILPN
                     PNMVAIKIPDNTPEIITGYSLDDEQALLAKVRYNRLIDIFLGITTYSLQNHLRTTVKGI
                     GQIEIDELYVGIDKYGCHYIIPVQAKGGTDQISMVQTSQDTAWCEQKYPNVRCRAISAQ
                     FVSSDLIALFELKIDEGQVKLIEERHYKLVPAKELDSSDISNYRMSV"
                     /name="hypothetical protein"
     gene            complement(1247..1438)
                     /locus_tag="GUD44_23395"
     CDS             complement(1247..1438)
                     /locus_tag="GUD44_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UPX62695.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1503..1826
                     /locus_tag="GUD44_23400"
     CDS             1503..1826
                     /locus_tag="GUD44_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UPX62697.1"
                     /translation="MITIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQMAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQGVR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2035..2466
                     /locus_tag="GUD44_23405"
                     /pseudo=""
     CDS             2035..2466
                     /locus_tag="GUD44_23405"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2504..3022
                     /locus_tag="GUD44_23410"
     CDS             2504..3022
                     /locus_tag="GUD44_23410"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UPX62696.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQ
                     GKEWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            3029..3262
                     /locus_tag="GUD44_23415"
     CDS             3029..3262
                     /locus_tag="GUD44_23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UPX62692.1"
                     /translation="MTELETHLLSALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3653..4903
                     /locus_tag="GUD44_23420"
     CDS             3653..4903
                     /locus_tag="GUD44_23420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="UPX62693.1"
                     /translation="MTRPIAVDLFAGAGGLSLGFEQAGFDIMASVEIDPIHCSAHKYNF
                     PMCKTICRSVVDVTGEYVREVSGIGEKEIDVVIGGAPCQGFSLIGKRALDDDRNKLVYH
                     YVRLVLELNPKYFIFENVKGLTVGKHKQFLVEIMDAFKNGGYDVVSDYKVLNAADYGVP
                     QDRRRLFLMGGRKGITLPSYPEPLSIRTTVGDAIGDIPDAEKYPELWERDWVDAKFGEQ
                     SLYSSYLHGVRKDPSDFSYPRIFNKDKLTCSLLTDHSDISRGRFVETLPDTVEPISRFK
                     KLSMNGLCNTLRAGTASDRGAFTSPRPIHPIYPRVITVREAARLHSYPDWFRFHVTKWH
                     GFRQVGNSVPPMLARSVGLQIMKVLGVTPKKPTKKIKLGDESLVAISMSDAASMFGVSS
                     NVIAKRIKNSNKEVVCE"
                     /name="DNA cytosine methyltransferase"
     Typing          1074..1159
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="100.0"
                     /evalue="3.0800000000000004e-27"
     Typing          complement(523..1063)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.522"
                     /coverage="100.0"
                     /evalue="1.75e-177"
     Typing          1471..1820
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.453"
                     /coverage="99.0"
                     /evalue="7.13e-64"
     Typing          1817..3366
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.466"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaaccgatca aatatcaatg gtgcaaacat cgcaagatac agcgtggtgt gaacaaaaat
       61 acccaaatgt tagatgtaga gctatttccg cccagtttgt ttcgagcgat ttgattgcac
      121 tgtttgagct gaagatagac gaaggacaag tgaaattgat tgaggaaagg cattacaaac
      181 tggttccggc aaaagagcta gatagctctg acatctcaaa ctaccgcatg tctgtatgat
      241 ttctgagagg atcttcttga gatcctttcg ttctagccgt aatctcttaa gctgtaaaca
      301 aaaaaaccac cgctaccaac ggtggttcgt ttgccggaat ttagagctac caactcttcc
      361 ttccgaaggt aactggcttt cacggagcgc agataccaaa actgtccttt tagtgtagcc
      421 gtagtcaggc caccacttca agaactctgt agataactct actccgctct gctccgtgaa
      481 tccgttacct gtggccgttg ccattggcgc ttttgcatgt ctttccgggt tggactcaag
      541 tcgatagtta ccggaaaagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc
      601 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gaaaaacgcg
      661 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
      721 agccaccagg gggaaacgcc tggtatcttt aagtcctgtc gggtttcgcc acctctgact
      781 tgagcatcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcgg
      841 cgcggccttc ttcctgtccc tggctttttg ctcacatgtt ctttccggtg ttatcccctg
      901 attctgtgga taaccgcatc accgtctttg agtgagctga taccgctcgc cgcagccgaa
      961 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtatgttc
     1021 tccttacgca tctgtgcggc atttcacacc ggacatggtg cacttctcat acaatccgca
     1081 gcgatgcaaa atgagataag ccagtataca ctccgctatc gctacgtgac tgggtcaggg
     1141 ctgcgccccg acacccgcta aaacccgctg acgcgccctg gcgggcttgt ctgctcccgg
     1201 catccgctca cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     1261 cgtcatcccc gaaacgtgcg aggcagctgc ggtaaagctc gtcggcgtgg tcgtgaagtg
     1321 attcacaaat atcggcctgt tcatctgcgt ccagttcgtt gagtttctcc agcagcgtta
     1381 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttagtcactg
     1441 atgcctccgt gtaaggggga tttctgctca tgggggtaat gataccgatg aaacgcgaga
     1501 ggatgatcac gatacgggtt actgatgatg aacatgcccg tttgctggaa cgttgtgagg
     1561 gtaaacaact ggcggtatgg atgcggcggg tctgcctggg tgagccggtt gcccgttccg
     1621 ggaagctgcc gacactggca ccgccgttac tgcgtcagat ggccgccatc gggaataacc
     1681 tgaatcagac agcgaggaag gtgaacagcg ggcagtggtc ttccggtgac cgggttcagg
     1741 tggtggccgc actgatggcc atcgagagag agttgcgcag tttacggcag gtcgtcaggg
     1801 aacagggagt gcgggatgat tgttaaattt catgccaggg gaaaaggcgg tggcagtggt
     1861 ccggttgatt acctgctggg aagggagcga aaccgggaag gcgcaacggt gcttcagggt
     1921 aatccggaag aagtccggga actcatcgat gccacgccat ttgcgaagaa atacacgtcc
     1981 ggtgttctgt cgtttgcgga gaaagaactg ccgcctggag gacgtgaaaa agtgatggcg
     2041 agctttgagc gtgtactgat gcccggtctt gaaaaaaatc agtacagcat tctgtgggtg
     2101 gagcaccagg acaaggggcg gcttgagctg aattttgtca ttccgaacat ggagctggag
     2161 accggaaaac gcctccagcc gtactacgac cgcgcagaca gaccgagaat tgatgcctgg
     2221 cagacgctgg ttaatcacca ttacgggctg catgacccca acgccccgga gaaccgccgg
     2281 atactgactc tccctgataa cctgccggaa aagaaacagg cgcttgctga gggagtcaca
     2341 cgaggtatag acgcacttta ccatgccgga gagataaaag gccgtcagga tgtgattcag
     2401 gcgctcacgg aggcggggct ggaagtggtc agggtgacgc gaagcagtat cagcattgca
     2461 gacccgaacg gcgggaggaa tatcagactg aaaggagcat tttatgagca atcttttaca
     2521 gacgggcgcg gagttcgaga aaaagctgaa agagagagcc gaatctacag agaaaatgct
     2581 gaacaacgag ttcaggaggc tcggcgaatc tgtaagcgag gctgtgacat caaacgagac
     2641 gaaaatcaga gacgctatag ccctgttcac cgcctcgaca gaggaatcgc tggaaaaaca
     2701 ccgggaaggg gtgaaagagg cgatgaggca gcacaggaga gacgtgttaa agctggcagg
     2761 gaatacgggc atgatgttac tggggatagt ctttctcctg tttaccgcga gtggcgggac
     2821 gctctggtat cttggcgggc ggatacaggc gaacctggaa gatatcagga agcaggaaga
     2881 gacattgcag aaactgaacg cgaagacatg gggtgtggag tttgtgcagg acgggaacag
     2941 gaaatttctg gtccttccgt acgggaagtc agcagaggtg atttcctttc aggggaagga
     3001 atgggtacaa ctgacggagt gacacagagt gacagagctg gaaacacatt tgctgagcgc
     3061 cttagagcag ctgcaacagg actatatgca gcggctgagc gaatgggaga gcgccttcgt
     3121 ggaattgcag aagatgtttt cgcttacgca acgggacaac gcgatgctga acgagcgggt
     3181 catgcagttg agtcagcagg tgcagcactt gagcgagcag acagaacgct tgagccagtt
     3241 atacagcgag aactggagat aagagaggaa cggctgatac aggagcgaga acatgcgtta
     3301 tccctggaac gggagcgtca gccggaaata caggaacgca cgctggatgg tccttcgctg
     3361 ggatggtgaa accatgaaaa atggcagctt cagtggatta agaggggtta atgtggcctg
     3421 taccatctgg ttgcataggt attcatgcgg ttaaaattta tcaggcgcga tcgcggcagt
     3481 ttttcgggtg gtttgttgcc atttttacct gtctgctgcc gtgatcgcac cgaacgcgtt
     3541 tctgcggtgc gggcaagaga tgttatgttc aatcggaatc agaaaaacga tcggtaaatc
     3601 gatcgttttt ctgatagtat cacgttgatt ttatataacg tggatgtaaa gaatgactag
     3661 accgattgct gttgatttat ttgctggagc gggtgggctg agtctcggtt tcgagcaagc
     3721 tggctttgat ataatggcat cagttgaaat agatccaata cattgctctg ctcacaaata
     3781 taactttcct atgtgcaaaa cgatatgtag aagtgttgtt gatgttaccg gagaatacgt
     3841 tagggaagtt tcaggtattg gtgagaaaga gattgatgta gtgatcggag gcgctccatg
     3901 ccaaggcttt tcattaatag gaaagcgcgc gcttgatgat gatagaaaca aattggtcta
     3961 tcactatgta cggctggtgc ttgagctaaa ccctaaatac ttcatttttg aaaatgtaaa
     4021 aggattaacg gtaggtaagc ataagcagtt tttagtagag ataatggatg catttaaaaa
     4081 tggcggatac gacgttgtaa gcgattataa agtgctaaat gcagctgatt atggggtccc
     4141 tcaagatcgc cgtcgtcttt tcttaatggg tggaaggaaa ggaattactt tgccatcata
     4201 cccagaacca ttatccatta gaacaacggt tggggacgct attggggata ttcctgacgc
     4261 agaaaaatat cctgagctat gggagaggga ttgggttgat gctaaatttg gtgagcaatc
     4321 attatactca tcctatttgc atggcgtaag aaaagaccca agtgatttta gctacccaag
     4381 aatttttaat aaagataaat taacgtgttc tttactgaca gatcactctg acatttctag
     4441 aggacggttt gttgaaactt tgcctgacac ggtagaacca atcagcaggt tcaaaaagct
     4501 ctcaatgaat gggctatgta atactttaag ggcaggaaca gcaagtgata gaggtgcttt
     4561 tacatctcct cgccctatac atccgatcta tcctagggtc attacggtta gagaggcagc
     4621 aagactgcat tcatatcctg attggttcag atttcatgtg actaagtggc atggatttag
     4681 acaggtcggt aatagtgttc ctcccatgct agctagaagt gttggcttgc agattatgaa
     4741 ggttcttggc gttacgccaa aaaaaccaac gaagaaaatc aaactcggtg atgaaagttt
     4801 ggttgctatc tctatgtccg atgctgcatc tatgtttggt gtgtcatcaa atgtaattgc
     4861 taagagaata aaaaatagta acaaggaggt tgtttgtgag taaggataaa aaaggtaggt
     4921 caaaaaatag ataccatcag ttggtagaaa agatattttt cgataaatat gttgatggtg
     4981 acttagaggt attgtttgaa aggttagaga taaacagcgc tgcaaaggat ttgggtatag
     5041 atctcccttc caatgttggt gatgttatat attcaatcag atttagaaca cctatgccag
     5101 aaagtatttt attgactcaa ccagaaggta aggagtggat tattgagctg gcgggtaggt
     5161 caaagtatcg atttagattg gtaactatta gtaggatttt gccaaatcca aatatggtgg
     5221 caataaaaat cccagataat accccggaga taatcacagg ttattctcta gacgacgaac
     5281 aggcattgct ggcgaaggtg aggtataacc gattaataga tatatttcta ggtataacta
     5341 catattcttt acagaaccat ttaagaacta cggtgaaggg aattggtcaa atagagatcg
     5401 atgaacttta tgtcgggata gataaatacg gttgccatta tataatacca gtccaagcaa
     5461 aaggtg
//
