LOCUS       CP093133               10384 bp    DNA     circular BCT 07-APR-2022
DEFINITION  Salmonella enterica subsp. enterica serovar Reading strain
            PNUSAS010176 plasmid pPNUSAS014950_1, complete sequence.
ACCESSION   CP093133
VERSION     CP093133.1
DBLINK      BioProject: PRJNA230403
            BioSample: SAMN07193435
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Reading
  ORGANISM  Salmonella enterica subsp. enterica serovar Reading
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Webb,H.E., Kim,J.Y., Tagg,K.A., Miller,E.A., Johnson,T.J., de la
            Cruz,F., Penil-Celis,A. and Folster,J.P.
  TITLE     Five complete Salmonella enterica serotype Reading genomes recovered
            from patients in the United States
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   Tarr,C.L., Trees,E., Katz,L.S., Carleton-Romer,H.A., Stroika,S.,
            Kucerova,Z., Roache,K.F., Sabol,A.L., Besser,J. and Gerner-Smidt,P.
  CONSRTM   PulseNet: The National Subtyping Network for Foodborne Disease
            Surveillance
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2018) Enteric Diseases Laboratory Branch, Centers
            for Disease Control and Prevention, 1600 Clifton Rd., MS C03,
            Atlanta, GA 30333, USA
REFERENCE   3  (bases 1 to 10384)
  AUTHORS   Webb,H.E., Kim,J.Y., Tagg,K.A., Miller,E.A., Johnson,T.J., de la
            Cruz,F., Penil-Celis,A. and Folster,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2022) EDLB, Centers for Disease Control and
            Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 44x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/06/2022 15:22:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,538
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,282
            CDSs (with protein)               :: 4,282
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 96 of 136
            Pseudo Genes (internal stop)      :: 31 of 136
            Pseudo Genes (multiple problems)  :: 46 of 136
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Salmonella enterica subsp. enterica serovar
                     Reading"
                     /mol_type="genomic DNA"
                     /strain="PNUSAS010176"
                     /serovar="Reading"
                     /isolation_source="stool"
                     /sub_species="enterica"
                     /db_xref="taxon:165302"
                     /plasmid="pPNUSAS014950_1"
                     /geo_loc_name="USA"
                     /collection_date="2017-05"
                     /collected_by="CDC"
     gene            join(10206..10384,1..385)
                     /locus_tag="CDI94_22645"
     CDS             join(10206..10384,1..385)
                     /locus_tag="CDI94_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOI52591.1"
                     /translation="MHALSILPSVARANLATKFSSHLKVIELFNTMQDSQVVVLSSLIE
                     GHHLTSSGNSVKADFEVTRLPAIIEMLEKKYFFPIRHLNVSVKSVTTGRMTGQTVYFIE
                     PEHIEQLLADPELVFANQERSLFFRSLEKEGKNLGKLIEKKGSVSQAVLSLLHHAYKDK
                     PLSDEMWKEIEDKFTHMLDELSAA"
                     /name="hypothetical protein"
     gene            complement(414..1040)
                     /locus_tag="CDI94_22650"
     CDS             complement(414..1040)
                     /locus_tag="CDI94_22650"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOI52592.1"
                     /translation="MSPTLNKQASKERCAVEFATRKKSKTQRKKSKALSYRGLCFFCTL
                     VTGKCQWTSKMSKQDYIQLRTLTDQFYADNKGLQEALDGSNDGKVRGYGIVVIDLNGLV
                     FGIPLRSHLNHKFGFVSERSEGVKKGLDYTKALLIKKEEYVSRAYKIPTPEFTHINDNK
                     EKIQEDFNKFVNRYIEANVKKDENILRNYRYSTLKNYHKELGLED"
                     /name="hypothetical protein"
     gene            2063..2383
                     /locus_tag="CDI94_22655"
     CDS             2063..2383
                     /locus_tag="CDI94_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UOI52593.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNTGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGND
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2592..3026
                     /locus_tag="CDI94_22660"
                     /pseudo=""
     CDS             2592..3026
                     /locus_tag="CDI94_22660"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3061..3546
                     /locus_tag="CDI94_22665"
     CDS             3061..3546
                     /locus_tag="CDI94_22665"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UOI52594.1"
                     /translation="MSSLLALAKDLEKQSKAQQQNTCEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEQKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            3550..3762
                     /locus_tag="CDI94_22670"
     CDS             3550..3762
                     /locus_tag="CDI94_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UOI52595.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4433..5293)
                     /locus_tag="CDI94_22675"
     CDS             complement(4433..5293)
                     /locus_tag="CDI94_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="UOI52596.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="class A broad-spectrum beta-lactamase TEM-1"
     gene            complement(5476..6033)
                     /locus_tag="CDI94_22680"
     CDS             complement(5476..6033)
                     /locus_tag="CDI94_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UOI52589.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            6197..9202
                     /locus_tag="CDI94_22685"
     CDS             6197..9202
                     /locus_tag="CDI94_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="UOI52597.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            9538..9948
                     /locus_tag="CDI94_22690"
     CDS             9538..9948
                     /locus_tag="CDI94_22690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOI52590.1"
                     /translation="MAFISLSNLAAKTGRIIQSQCVTATQTKKSTGAVELGFRLSKSAM
                     DETGITIGDRVDILYDKEENQWMIKKISEGGFGVSGQKNKHGGYSSCAVRLTLRPGFPR
                     LSEDEEDNKKLLSDDSFTQLSEGVIVFRLDDE"
                     /name="hypothetical protein"
     ARG             complement(4437..5293)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1776..1955
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.339"
                     /coverage="83.0"
                     /evalue="7.539999999999999e-48"
     Typing          complement(1317..1830)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.217"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2374..3878
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.426"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgaggtcact cgcttaccgg cgattatcga gatgttagag aaaaaatatt ttttccctat
       61 tcgtcatcta aatgtatctg ttaaatctgt aactacagga aggatgactg gacaaactgt
      121 ctatttcata gagcctgaac acatcgagca acttctcgct gatcctgagc ttgtgtttgc
      181 taatcaggaa aggagcctct tctttcgttc tctcgaaaaa gaagggaaaa atctcggcaa
      241 gcttatcgag aaaaaaggta gtgtttctca agccgtctta agccttctac atcacgccta
      301 caaagataaa cctctttctg acgagatgtg gaaggagata gaagataagt tcacccatat
      361 gctggatgaa ttgagcgcgg cgtaaaggca agggcggtcg ttagaccgcc tttttagtct
      421 tctaaaccta attctttgtg atagtttttc agcgtagagt aacgatagtt tctgagtatg
      481 ttttcatctt tcttgacgtt cgcttctatg tatctgttta cgaatttgtt gaaatcttct
      541 tgtatttttt ctttgttatc attgatatgg gtaaattcag gagttggtat cttataggct
      601 cgcgatacat actcttcttt cttaattagc aacgctttgg tgtaatccag tcctttctta
      661 acaccttctg atcgctccga aacgaatcca aatttatggt ttaaatggct tcgtaagggt
      721 atcccgaaca ccaatccgtt gagatcaata actacgattc cgtagcctct tacttttcca
      781 tcattactgc cgtcgagagc ctcttgtagg cctttgttat cagcataaaa ctggtctgta
      841 agggttctta actgtatgta atcctgtttg ctcatttttg atgtccattg gcattttcct
      901 gtaaccaatg tacaaaaaaa gcaaagccct ctgtaagaga gggctttgct tttctttcgt
      961 tgagtcttgg attttttacg tgtggcgaac tctaccgcac atctttcttt ggaagcttgt
     1021 ttgtttaacg tggggctcat tcctaccgcc acttctagat ttacttatat caaaaattat
     1081 atgtgaagtc aatttcgtag ctgttcttga ctggacttac tttgccagcg taatcttctc
     1141 ctgaaacgaa aaaaccaccc tggcaggtgg tttttcgaag gttatttaat cctggcagat
     1201 tatctaaccg tggtaacagt cttgtgcgag acgtgtcacc aaatccgtcc ttccagtgta
     1261 gcctcagtaa ggccaccact tcaagaactc tcaatacatc tctcgcacat catgtttacc
     1321 agtggccgtt gccaatggcg attagtcgtg tctttccggg ttggactcaa gtcgatagtt
     1381 accggaaaag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga
     1441 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agataaacgc ggccataaca
     1501 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag
     1561 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
     1621 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcagc gccgcatcct
     1681 tttcttgccc tgtttgtcat gccgtatgcc tttgctctta tccgtgtgca ggtaacgccc
     1741 gccgcagccg aacggcagga gcggaagcga ccgagtgagc gaggaggcgt aatatcaccg
     1801 caacctccat gttctcccgt acgccctgca gctgcatttt cccctgccac atgaagcact
     1861 tttataaagt gttactccgc cgtaacatat taagccagta tacactccgc tagcgctacg
     1921 tgactggttc agggctgcgc cccgaaaccc gctaaaagcc actgacgcgc ctgcggcttg
     1981 tccaacaccg tgccgaccgg gaaacatttc ccgcccgtcc cggtgttatc aggaggctac
     2041 tctggcagac aaacgcaaca agatgctcac gatgtgggtg acggaagacg aacaccggcg
     2101 gctgctcgag cggtgcgacg gcaagcagct ggcggcgtgg atgcgtcaga cctgcctcga
     2161 tgagaagccg gcgcgtgccg gcagactgcc gtcgatatcg ccggcgctgc tgcgccagct
     2221 tgccggtatg ggtaataacc tgaaccagat cgcccggaaa gtgaataccg gtggtgccgg
     2281 tcatgaccgg gtgcagatcg tggccgccct gatggcaatc gacgcgggac tggagcggct
     2341 gcggcatgcg gtgctggaaa aaggggggaa tgatgatcgt taaattccat ccccgggggc
     2401 gcggtggtgg tgccggtccg gtggattacc tgctgggaaa agaccggcag cgcgaggggg
     2461 cgaccgtgct gcagggaaag ccggaggaag tccgggagct tatcgatgcc tcgccgtatg
     2521 cgaaaaagta cacctccggc gtactgtcgt tcgcggaggc tgagctgccg cccgggcagc
     2581 gggagcagat catggccagc tttgagcggg ttctgatgcc cgggctcgat aaagaccagt
     2641 acagcattct gtgggtggag cacaccgaca aggggcggct ggagctgaat ttcctgatcc
     2701 ccaacacaga gctgctgacc gggaaacgcc tgcagccgta ttacgatcgc gccgaccggc
     2761 cgcgcatcga tgcctggcag accgtggtga acggcagact ggggctgcat gatccaaacg
     2821 ccccgaagaa ccggcggtta ctggtcacgc cgtccgcact gccggaaacg aagctggaag
     2881 ccgctcaggc gattacacgg ggtttgctgg ccttagcctc atccggggcg ctcaaaacgc
     2941 gcgaggacgt cacagggtcg ctcactgcgg caggttttga ggtggtccgc accacaaaga
     3001 acagtatcag cattgccgac ccggacgggg ggcgaaacat ccgactgaag ggagccattt
     3061 atgagcagtc ttttagcgct ggcgaaggac ttggaaaagc aatcgaaagc gcagcagcag
     3121 aatacctgcg agatgctgaa agccgcattc agcgagcacg agaagtctgt aaaagcggaa
     3181 ctgagcgcaa gcgcgaagag aatcagcgac gccatcagcg cccacgagca gggtatgacc
     3241 gcggcgatgc agtcgaaccg gctgagcgtg ctgcgtatgg ttggccggac gtggctgacc
     3301 atcaccctgg tctcagtact gctgatcgcc acgagcggga gcattctgtg gtggcagggg
     3361 cagcagatca ccgggaatta ccagactatc cgggcgcagg agcggacgca ggccatgctg
     3421 tcggagaaga accacggcgt gcagctgtcg ctctgtgggg aacagaaact gtcctgcgtc
     3481 aaagtgaacc cgaaagcggg agcttacggg gaggaaggga actggatggt actggagagg
     3541 aaatagccca tgacggaact ggaaaaacag ttgctgaccg cattagagca gctacatcag
     3601 gactactcgc aaaggctgga cgagtgggag agcgccttcg cggaatggca gaagatgtct
     3661 ggtcttatgc aacgggagaa cgcggcgctg aacgagcgcg tcacgcgctt gagtcagcag
     3721 gtggcgaact tgagcaggca gttacagcgt ttgagccagt agtgcagcgg catgaaatgg
     3781 cggtggccgc cgaacgggca tacgagcagc gacagcatga aaaagagctg gcagaacccc
     3841 gtttccgtca gcggagctat aacggtccga cgctgggctg actgcgcgca tccagtgaca
     3901 aaccccaaga agaagggctc ataaatttcc cgctaaatac ggtcgtgatg aaaagatcac
     3961 ggctcaagag agggggattt atgaagatac taaaagcgct cagtctgata ctgcaatgcg
     4021 tcaaactggc aatggatctg tgggagcgat tcgggggaaa gtggcctttt tagtgcttta
     4081 caccgtattt atacgcgaaa actgctgtca ttcatctggc tgcataacca tgcagccaag
     4141 tgaaatttat ctggcgtaaa cggggcggtt taccgggtgc tttgctaggg gtttcacccg
     4201 acaggcaccg tatttgcgcc gaaccagccc gaagcggtgc gggcaagaac tcttatgtta
     4261 aattcatctc atcggcaacc tatacggggt ctgacgctca gtggaacgaa aactcacgtt
     4321 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4381 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4441 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4501 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4561 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4621 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4681 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4741 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4801 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4861 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4921 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4981 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5041 cggcgtcaac acgggataat accgcaccac atagcagaac tttaaaagtg ctcatcattg
     5101 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5161 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5221 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5281 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5341 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5401 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5461 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attttataaa ccgtggagcg
     5521 ggcaatactg agctgatgag caatttccgt tgcaccagtg cccttctgat gaagcgtcag
     5581 cacgacgttc ctgtccacgg tacgcctgcg gccaaatttg attcctttca gctttgcttc
     5641 ctgtcggccc tcattcgtgc gttctaggat cctccggcgt tcagcctgtg ccacagccga
     5701 caggatggtg accaccattt gccccatatc accgtcggta ctgatcccgt catcaatgaa
     5761 ccggactgcc acgccctgag cgtcaaattc ctttatcagt tggatcatat cggcagtgtc
     5821 gcggccaaga cggtcgagct tcttaaccag aatgacatca ccttcctcca ccttcatcct
     5881 cagcaaatcc agcccttccc ggtctgttga actgccggat gccttatcgg taaatatacg
     5941 gtttgctttc acacctgcgt ctttgagtgc tctgacctga agatcaagag actgctgact
     6001 ggttgagacc cgagcgtaac caaaaagtcg cataaaaatg taccttaaat cgaatatcgg
     6061 acaactcatg tctattatta caaatttacg atttaataga catattaatg taacagtttt
     6121 acgatgtccg ataatttata acatttcgta cggttgcaaa aatgttacta aatgcccgtc
     6181 aggcagggag gccgatatgc ccgttgactt tctgaccact gagcagactg aaagctatgg
     6241 cagattcacc ggtgaaccgg atgagcttca gctggcacga tattttcacc ttgatgaagc
     6301 agacaaggaa tttatcggaa aaagcagagg tgatcacaac cgtctgggca ttgccctgca
     6361 aattggatgt gtccgttttc tgggcacctt cctcaccgat atgaatcata ttccttccgg
     6421 cgtccggcat tttaccgcca gacagctcgg gattcgtgat atcaccgttc ttgcagaata
     6481 cggtcagagg gaaaataccc gccgtgagca tgcagcgctg atacgtcagc actatcagta
     6541 tcgtgaattt gcctggccct ggacatttcg ccttacccgt cttttatata cccggagctg
     6601 gataagcaac gaacgtcctg gcctgctttt cgatctggcg acagggtggc ttatgcaaca
     6661 tcgtattatt ctccccggag ccactacgct gacccggttg atttcagagg taagggaaaa
     6721 ggcgacgttg cgcctgtgga acaaactggc actgataccg tcagccgaac agcgttcaca
     6781 gctggagatg ctgctggggc caactgattg cagccgcctg tctttactgg aatcactgaa
     6841 aaagggccct gtgaccatca gtggtccggc gtttaatgaa gcaattgaac gctggaaaac
     6901 tctgaacgat tttggcctgc atgctgaaaa cctgagtaca ctcccggctg tgcgcctgaa
     6961 aaatctcgca cgttatgctg gtatgacttc ggtgttcaat attgccagga tgtcaccgca
     7021 gaaaaggatg gcggttctgg ttgcctttgt ccttgcatgg gaaacgctgg cgctggatga
     7081 tgcattggac gttctggacg ccatgctggc cgttatcatc cgtgacgcca gaaagattgg
     7141 gcagaaaaaa cggctccgct cgctgaagga tctggataaa tctgcattgg cgctcgccag
     7201 cgcatgttcg tacctgctga aagaagaaac accggacgaa tcgattcgtg ctgaggtgtt
     7261 cagctacatc ccaaggcaaa agctggctga aatcatcacg cttgtccgtg aaattgcccg
     7321 gccctcagac gataattttc atgaagaaat ggtggagcag tacgggcgcg ttcgtcgttt
     7381 cctgccccat ctgctgaata ccgttaaatt ttcatccgca cctgccgggg ttaccactct
     7441 gaatgcctgt gactacctca gccgggagtt cagctcacgg cggcagtttt ttgacgacgc
     7501 accaacggaa attatcagtc ggtcatggaa acggctggtg attaacaagg aaaaacatat
     7561 cacccgcagg ggatacacgc tctgctttct cagtaaactg caggatagtc tgaggcggag
     7621 ggatgtctac gttaccggca gtaaccggtg gggagatcct cgtgcaagat tactacaggg
     7681 tgctgactgg caggcaaacc ggattaaggt ttatcgttct ttggggcacc cgacagaccc
     7741 gcaggaagca ataaaatctc tgggtcatca gcttgatagt cgttacagac aggttgctgc
     7801 acgtctttgc gaaaatgagg ctgtcgaact cgatgtttct ggcccgaagc cccggttgac
     7861 aatttctccc ctcgccagtc ttgatgagcc ggacagtctg aaacgactga gcaaaatgat
     7921 cagtgatcta ctccctccgg tggatttaac ggagttgctg ctcgaaatta acgcccatac
     7981 cggatttgct gatgagtttt tccatgctag tgaagccagt gccagagttg atgatctgcc
     8041 cgtcagcatc agcgccgtgc tgatggctga agcctgcaat atcggtctgg aaccactgat
     8101 cagatcaaat gttcctgcac tgacccgaca ccggctgaac tggacaaaag cgaactatct
     8161 gcgggctgaa actatcacca gcgctaatgc cagactggtt gattttcagg caacgctgcc
     8221 actggcacag atatggggtg gaggagaagt ggcatctgca gatggaatgc gctttgttac
     8281 gccagtcaga acaatcaatg ccggaccgaa ccgcaaatac tttggtaata acagagggat
     8341 cacctggtac aactttgtgt ccgatcagta ttccggcttt catggcatcg ttataccggg
     8401 gacgctgagg gactctatct ttgtgctgga aggtcttctg gaacaggaga ccgggctgaa
     8461 tccaaccgaa attatgaccg atacagcagg tgccagcgaa cttgtctttg gccttttctg
     8521 gctgctggga taccagtttt ctccacgcct ggctgatgcc ggtgcttcgg ttttctggcg
     8581 aatggaccat gatgccgact atggcgtgct gaatgatatt gccagagggc aatcagatcc
     8641 ccgaaaaata gtccttcagt gggacgaaat gatccggacc gctggctccc tgaagctggg
     8701 caaagtacag gcttcagtgc tggtccgttc attgctgaaa agtgaacgtc cttccggact
     8761 gactcaggca atcattgaag tggggcgcat caacaaaacg ctgtatctgc ttaattatat
     8821 tgatgatgaa gattaccgcc ggcgcattct gacccagctt aatcggggag aaagtcgcca
     8881 tgccgttgcc agagccatct gtcacggtca aaaaggtgag ataagaaaac gatataccga
     8941 cggtcaggaa gatcaactgg gcgcactggg gctggtcact aacgccgtcg tgttatggaa
     9001 cactatttat atgcaggcag ccctggatca tctccgggcg cagggtgaaa cactgaatga
     9061 tgaagatatc gcacgcctct ccccgctttg ccacggacat atcaatatgc tcggccatta
     9121 ttccttcacg ctggcagaac tggtgaccaa aggacatctg agaccattaa aagaggcgtc
     9181 agaggcagaa aacgttgctt aacgtgagtt ttcgttccac tgagcgtcag acccctatac
     9241 aggcataaaa atcagtttct ataagcggct ttcttgcacc tacttggtga ttttttggat
     9301 gcgctgacgt tgctcgactt taaatcacca tagaaaaata ccctttactt acataaagat
     9361 cgatctggat gtgtatcttg acgaggttca atacgagcaa ttgtttttat atttaaggtt
     9421 tcttatcgcc gttttttgcg gctctagaaa aatcaataca aaagatcttg aaagccacct
     9481 cgctccctgg gtacacttcc gtataagaac tgatcgatgt ctaaaacgga ttgtattatg
     9541 gcttttatct ctctatcgaa cttggcagcg aagactggaa ggataatcca gtcgcagtgt
     9601 gttacggcca cgcaaacgaa aaaaagcacg ggcgctgtcg agctgggatt ccgcttgagc
     9661 aagagcgcta tggacgagac tggtattacc attggggatc gtgtcgacat tctttacgac
     9721 aaagaagaaa accagtggat gattaagaaa attagtgaag gtggctttgg tgtgtctggg
     9781 caaaaaaaca agcacggcgg ttactcatcg tgtgctgtta gattgacctt gcgaccaggt
     9841 tttcctcgcc tttctgagga tgaagaggac aacaagaagc tactttccga tgattcattc
     9901 actcagctaa gtgaaggtgt aatcgtcttc aggctggatg atgaatagga aggaccaaaa
     9961 aggaaaggca gagttagtga gcgtcgccaa acaatcgact aactccgcct tcttgtcaac
    10021 acatcactac ctttggaagg gtggagtgtt atgggcggca ttatgttcat aaacttgcat
    10081 tctaataaag aaaaaatcgt ttcttttgag ggatgtgaac acgacatttt ctattctgat
    10141 aggtgcgatg ctattggctt tggtctctta agtttatgtt tttaaaaaca tatgaggcta
    10201 tacatatgca cgctttatca atactacctt cagttgcccg cgctaacctg gcaactaaat
    10261 tctcgtctca tttgaaggtg atcgagctct tcaacacgat gcaggattcc caagttgttg
    10321 tcttgtcatc actgatcgaa ggccatcacc tcaccagttc tggtaacagt gtcaaagccg
    10381 actt
//
