LOCUS       CP093087                4633 bp    DNA     circular BCT 05-MAY-2022
DEFINITION  Salmonella enterica strain PNUSAS067730 plasmid pPNUSAS067730_1,
            complete sequence.
ACCESSION   CP093087
VERSION     CP093087.1
DBLINK      BioProject: PRJNA230403
            BioSample: SAMN11029788
KEYWORDS    .
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Webb,H.E., Kim,J.Y., Tagg,K.A., de la Cruz,F., Penil,A., Tolar,B.,
            Ellison,Z., Schwensohn,C., Brandenburg,J., Nichols,M. and
            Folster,J.P.
  TITLE     Twenty-one complete Salmonella enterica serotype Hadar genomes
            collected from patients in the United States
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Tarr,C.L., Trees,E., Katz,L.S., Carleton-Romer,H.A., Stroika,S.,
            Kucerova,Z., Roache,K.F., Sabol,A.L., Besser,J. and Gerner-Smidt,P.
  CONSRTM   PulseNet: The National Subtyping Network for Foodborne Disease
            Surveillance
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2019) Enteric Diseases Laboratory Branch, Centers
            for Disease Control and Prevention, 1600 Clifton Rd., MS C03,
            Atlanta, GA 30333, USA
REFERENCE   3  (bases 1 to 4633)
  AUTHORS   Webb,H.E., Kim,J.Y., Tagg,K.A., de la Cruz,F., Penil,A., Tolar,B.,
            Ellison,Z., Schwensohn,C., Brandenburg,J., Nichols,M. and
            Folster,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2022) EDLB, Centers for Disease Control and
            Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Assembly Name                     :: PNUSAS067730_consensus
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2022 09:21:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,638
            CDSs (total)                      :: 4,513
            Genes (coding)                    :: 4,394
            CDSs (with protein)               :: 4,394
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 19
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 46 of 119
            Pseudo Genes (incomplete)         :: 90 of 119
            Pseudo Genes (internal stop)      :: 22 of 119
            Pseudo Genes (multiple problems)  :: 35 of 119
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="PNUSAS067730"
                     /db_xref="taxon:28901"
                     /plasmid="pPNUSAS067730_1"
                     /geo_loc_name="USA"
                     /collected_by="CDC"
     gene            complement(386..577)
                     /locus_tag="EZ266_23165"
     CDS             complement(386..577)
                     /locus_tag="EZ266_23165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UPX67133.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            640..963
                     /locus_tag="EZ266_23170"
     CDS             640..963
                     /locus_tag="EZ266_23170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UPX67136.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1172..1612
                     /locus_tag="EZ266_23175"
                     /pseudo=""
     CDS             1172..1612
                     /locus_tag="EZ266_23175"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1641..2201
                     /locus_tag="EZ266_23180"
     CDS             1641..2201
                     /locus_tag="EZ266_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UPX67134.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTA
                     LSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2208..2441
                     /locus_tag="EZ266_23185"
     CDS             2208..2441
                     /locus_tag="EZ266_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UPX67135.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3169..4008)
                     /locus_tag="EZ266_23190"
     CDS             complement(3169..4008)
                     /locus_tag="EZ266_23190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UPX67132.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     Typing          214..298
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(7..194)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.105"
                     /coverage="95.0"
                     /evalue="1.56e-68"
     Typing          4008..4459
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          954..2539
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.811"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggccttttg ctcacatgtt tcttttggcg ttatcccctg attctgtgga taaccgcatc
       61 accgtctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca
      121 gtgagcgagg aagcggaaga gcgcctgatg cggcattttc tccttacgca tctgtgcggt
      181 atttcacacc gcatatggtg cacttttctt ataatccgca ctgatgccac atagttaagc
      241 cagtatacac tccgctatcg ctacgtgact gggtcagggc tgcgccccga cacccgctaa
      301 aacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgctcac aggcaagctg
      361 tgaacgtctc cgggagctgc atgtttcagt tgctttcacc gtcatcgccg aagcgtgcga
      421 ggcaactacg gtagagttcg tcggcgtggt cgtgaagtga ttcgcagatg tctgcctgtt
      481 cgtccgcgtc cagctcgttg agtttttcca gcagggtcag cgtctggctt ctgataaagc
      541 gggccatgtt aagggctgtt ttttcctgtt tggtcatggt cttaaggctc cttaataggg
      601 gattttttag ggggaatgat accgatgaaa cgcgagaaga tgctcacgat acgggttagc
      661 gatgaggagc acgcccggtt actggaacgc tgcgagggta aacgactggc agaatggatg
      721 cggcgggtct gtctggggga gccggttgcg cgcacaggaa aactgccgac actatcgccg
      781 ccattgctgc ggcatctggc tgccatcggg aacaatctga accagacagc ccggaaggtg
      841 aacagcggcc actggtcttc gattgaccgg gttcatgtgg tggccgcact gatggccatc
      901 gagggtgaat tgcgccagtt gcggcaggct gtcagggaac aggggggacg ggatgatagt
      961 taaatttcat gccaggggga aaggcggtgg aagtggcccg gtcgattacc tgctggggcg
     1021 ggagcgtaac cgcgaaggcg caacggtgct ccggggtaac ccggaagaaa tccgggaact
     1081 cattgatgcc acgccatttt cgaagaaata cacgtcaggc gttctgtcgt tcgcggagaa
     1141 ggagttaccg ccgggtgaac gcgaaagagt gatgacgagc tttgagcgtg ttcttatgcc
     1201 cggtctcgaa aaagaccagt acagcattct ctgggtggaa caccaggata aggggcggct
     1261 cgaactgaat tttgtcattc cgaacatgga actacagagc ggtaagcgcc tccagccgta
     1321 ctacgaccgt gcggacaggc cgaggataaa tgcctggcag acgctggtta atcatcatta
     1381 cgggctgcgt gacccgaacg caccggagaa cagacggacg cttgtcacac cgaataatct
     1441 gccaaaagcg aaacaggagg ccgcagaagc gattacacgg ggtcttgagg cgctttaccg
     1501 tgccggagca ataaaaacgc gtcaggatgt cactgaggcg ctcacagcgg caggttttga
     1561 ggtggtgagg acaaccagaa gcagtatcag tattgccgac ccggaggggg ggcggaatct
     1621 caggctaaag ggggcgctgt atgagcaatc ttttacaaat gggaacagac ttcgagaaga
     1681 aactgaaaga gcgagccgct tctaccgaga acatgctgaa cagcgagttc aggaagctag
     1741 aagagtctgt agacaaggca ctgagcttaa acaggcagaa aatcagagac gctatcagcg
     1801 aacatacaac atccgtgaaa cagcaactgg atacgctgag caccaccgtg agcacgcagc
     1861 tcagcaccac agaggccgaa ctgtcccggc agcagaaaaa cctgctctgg caggtgatca
     1921 aagggcgggt actgtttccg gcgctgaccg cgctcagcgt cacgggcggc attttcctgg
     1981 ggtgctgggg gctgattcag tggcaggaaa gcaggattgc gaaaaatatc ttgaccatca
     2041 gagagcagga aaacacgctg gcgaaactcg aggcaaaaac ctggggggtg acgttcgtca
     2101 atggcgagaa cgggaaattt ctggtgctgc cggacggggt gaaaggggaa aacacctgga
     2161 cggtgggcga caagaacgcc gtgaggctgg tcagggagta aacgagtatg acggaactgg
     2221 aagggcattt gctgaacgcc ttagagcatt tgcaacagga ctatatgcgg cggctgaacg
     2281 agtgggagag cgccttcgca gaattgcaga agatgcacgc ggttacgcaa cagaacaacg
     2341 cgatactgaa cgagcgtgtc gtgaacttga gtcagcaggt gcagctatta gccggacaag
     2401 tcgaccgctt gagtcggtta tttatcacga acaacagata agggatgaac aggagcgtca
     2461 ggcgcaggag cttgcccggc gctggcatga agagcaggaa cccgaagcgc ggatctggcg
     2521 tggcccgtca atggggatgt gaccggggtg ccccctgaaa tcgagccagt ttgtggcacg
     2581 ccctacccgg atggcgccaa tgttaatgta cgtacattag cattaacaat atccaaacct
     2641 ccccattttg ccccgttcga cggctggaac gtgcgaaact gatcgtcatt cagtttcgcg
     2701 cataactatg catgaggtta aaatttacca ggcgcgatcg cggcagtttt tcgggtggtt
     2761 tgttgctgtt tttacctgtc tgctgccgtg atcgcgctga acgcgtttaa gcggtacgcg
     2821 caatgcgacg ttatggtaaa tacggagtat tttaattgcg ggaattaaaa caacaaaggc
     2881 ccgctgtact ctatacagcg ggcctttgtt gtatatctct gtcgtttgtc cccgggggga
     2941 agcattactg ctaacaagtc ccgtgagaaa actgcgcaat cgtattgcgc accatgaacc
     3001 tattctcaac cgcaatctgg aggatgattt tgctacgata aagagaatta tcgcgtatcg
     3061 gtgtcagcat tcattggagt ggatgctgaa aaaccaggtg ttactccctc tacttacact
     3121 taaacctctc taaaaatata agtaagtact atcgagggct atcatgaact atggtgccct
     3181 ctgagcatag ctacccatta cttcttctat gtcattgaat aattcatttc ttaactctaa
     3241 gtattctagt aactccattt ctaatgactc tgtcgtgaac ttaaatctag aaggatcttt
     3301 gattgagttt actagtcttg ataacgctct atatgttttg agtgaaggtt gcattataga
     3361 tggaaagtat ttgaaaacct cttcatctct gttgaaatta actaaaaact tgtagtcatc
     3421 atttataaat ctcaagcata cagcgtcaag cttatcgcct tttcttatta ttatttcttt
     3481 ataggaaaga tatttttcag aagataagtc ccacatcaag cgaaaatctc tctttgcgaa
     3541 cgagttcgcg tcatcattga aaagagatga tgtgatggct ttggatagac cttctgtgtt
     3601 tatctctttg ttggatgggt agtaaatttt attaattgct ttcattctct caaatgatga
     3661 atataggtca ttatgccacc ttgttgaaat caccccttta gttatattct ccttgctaaa
     3721 attaagccct gtatcaaaaa taaatgcagc catcagtgaa tatgatatgt tttttattaa
     3781 ctcagactta aatggttccg ctatggagta gtacagtgat aaaataacta aaaataaaag
     3841 gtattgtctt acctttcccg atgagtatat gtaaaggcga tatttattta ttgatagttt
     3901 tattttgtat ggaatgatct catttctgtc atatcctctt cgtggtggag caaaacaaaa
     3961 gaaacatgca gctattaata aaacaaaaaa gatgaacagt gtaagcaagt tcggctcctg
     4021 tgtatgctca ctatgcatga ttgtcttgag atctaacgat cctgaaggta gtcttgtccg
     4081 taaacgaaaa aaccgcccag caaggcggct tttcgaaggt tctcagagca gcaactcttt
     4141 gaaccaaggt aactggcttg gaggagcaca gccaccaaat ctgtcctttc agtttagcct
     4201 taaccggcgc ataacttcaa gactaactcc tctaaacggt taccaatggc tgctgccagt
     4261 ggcgttttta cgtgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag
     4321 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4381 gggccgagat accaacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4441 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4501 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4561 ctgtttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag cggcgcggcc
     4621 ttcttcctgt tct
//
