LOCUS       CP091130                3223 bp    DNA     circular BCT 02-MAR-2022
DEFINITION  Serratia marcescens strain CM2015_854 plasmid unnamed5, complete
            sequence.
ACCESSION   CP091130
VERSION     CP091130.1
DBLINK      BioProject: PRJNA784350
            BioSample: SAMN23485804
KEYWORDS    .
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Allen,J.L., Doidge,N.P., Bushell,R., Browning,G.F. and Marenda,M.S.
  TITLE     Nosocomial infections caused by chlorhexidine-tolerant Serratia
            marcescens carrying a promiscuous multi-drug resistance IncHI2
            plasmid in a veterinary hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Allen,J.L., Doidge,N.P., Bushell,R., Browning,G.F. and Marenda,M.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2022) Faculty of Veterinary and Agricultural
            Sciences, The University of Melbourne, Cnr Flemington Rd & Park
            Drive, Parkville, Victoria 3010, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 12-MAY-2021
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 6x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/19/2022 10:45:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,875
            CDSs (total)                      :: 5,744
            Genes (coding)                    :: 5,623
            CDSs (with protein)               :: 5,623
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 18
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 47 of 122
            Pseudo Genes (incomplete)         :: 78 of 122
            Pseudo Genes (internal stop)      :: 24 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="CM2015_854"
                     /isolation_source="Wound fluid"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:615"
                     /plasmid="unnamed5"
                     /geo_loc_name="Australia: U-Vet Werribee Animal Hospital"
                     /collection_date="2015-09-28"
                     /note="unpigmented"
     gene            complement(1006..1338)
                     /locus_tag="L2D47_29355"
     CDS             complement(1006..1338)
                     /locus_tag="L2D47_29355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="UMK65538.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(1506..1721)
                     /locus_tag="L2D47_29360"
     CDS             complement(1506..1721)
                     /locus_tag="L2D47_29360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UMK65539.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1725..2210)
                     /locus_tag="L2D47_29365"
     CDS             complement(1725..2210)
                     /locus_tag="L2D47_29365"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UMK65537.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(2245..2679)
                     /locus_tag="L2D47_29370"
                     /pseudo=""
     CDS             complement(2245..2679)
                     /locus_tag="L2D47_29370"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2888..3211)
                     /locus_tag="L2D47_29375"
     CDS             complement(2888..3211)
                     /locus_tag="L2D47_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UMK65540.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             complement(1007..1338)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(85..276)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.821"
                     /coverage="89.0"
                     /evalue="7.28e-73"
     Typing          256..739
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(1409..2898)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtctgccaat ccggcctcct gataacgccg ggacggacgg gaaatctttt cccggtcggc
       61 acggtgttgg acaagccgca gacgcggcag tggttttagc gggtttcggg gcgcagccct
      121 gaaccagtca cgtagcgcta gcggagtgta tactggctaa acatgttatg acggagtggt
      181 aatttatgaa agtgcttcat gtgacagggg aaaaatgcgg ctgccgggcg taagtgcaaa
      241 atgcaggtgg tgatgaaatg acgcctcctc gctcactcgg tcgctacgct cctgccgtga
      301 gactgcggcg ggcgttacca gctcacagat tacgggataa gcaaatgccg ctcgaagcag
      361 gaaaggccaa gaaaaggatg cggagttgcc gtttttccat aggctccgcc cccctgacga
      421 gtatcacgaa aatgacgctc aaatcagcgg tggcgaaacc cgatgcggct taaagatacc
      481 aggcgtttcc ccctggtggc tccctcgtgc tcccccgcat tcctgccttt cggtttaccg
      541 gtgtcattcc gctgttatgg ccgcggtttc tcattccacg cctgaaactc ggttccgggt
      601 aggcagttcg ctccaggatg gactgtatgc acgaaccccc cgttcagtcc gactaccacg
      661 cccgttccgg taactatcaa cttgagtcca acccggaaag acacgacaaa tcgccagtgg
      721 cggtagccat tggtaactgg gttcacggag cagagattgt agagaatgcc tacagagttc
      781 ttgaagtgaa agcctgagtg cggctacact gaaaggacag tttaggtatc tgcgctccgt
      841 gaaagccagt taccatcgga acaaagagtt ggtagctctg aattccggca aacgaaccac
      901 cgttggtagc ggtggttttt ttgtttacag ggaaaaagat tacgcgcaga aagaaaggat
      961 ctcaaggaaa tccttttgct ttggccaata tgcttcggta aaaagttaat gcgcagaaga
     1021 ttttgaaaaa atattaataa ccaaaactcc aacgcaaata aggaacattc ctgcaattgc
     1081 gggccaatca agtttttgac cgctaaccaa ccaaccagct aggcttatta gtacaattcc
     1141 cgcaccagac caaattgcgt aagcaatacc ggtaggtata ttttccaatg tttgggaaag
     1201 cagataaaag gcagcgcaat aacttattgc agttcctaca gacggccaca ggcgtgtaaa
     1261 tccttctgaa tatttcataa aggtggtgcc tatgacttca gccacaatag cccctcccag
     1321 gaaaatatat gtattcataa aaatacctca tgttgaaaaa tttaaatatc tttacataac
     1381 aactaattaa tagaacccgg cttatcatag cgaaggacca tgatgctgcc actgcgatcg
     1441 ttccagctcc agcgctttct ggtgctgcac cgcccgctcc tgctgccggt gagtttctat
     1501 cgcgttcagc ctttcgacaa ccggcgcagc tgctcgctca aactctgcac ctgcctgctc
     1561 aagaccgtga cgcgctcgtt cagcgccgcg ttctcccgtt gaataagacc agacatcgtc
     1621 cgccattccg cgaaggcgtt ctcccactcg tccagccttt tcgagtagtc ctgctgtagc
     1681 tgctctaatg cgctcagcaa ctgtttttcc agctccgtca tgtgctattt ccccgccagt
     1741 atcatccagc tcgagtcctc tccgaactgt cccgcttccg ggttcaccct cacgcagcgg
     1801 cgtctctgct cgccgcaggt cgagagctgt acgccgctgt tcctctctga cagcatggcc
     1861 tgcgtgctct tctgctcccg gatggtcgta tagttctcga gtatctgctg cccctgccac
     1921 cacagaatgc ccgcgctcga cgctatcagc agcgcggaga ccagaacgat ggtcagccac
     1981 gtctggctga ccatgcgcag catccctttc gtgcgctggc tcatggctga ggacagcttc
     2041 cggtcgtggt cgaggatggc ggcgctgatt ctcttctcgc tttcgctcag ttccgctctg
     2101 acagacttct cgtgctcgct gaatgcggct ttcagcatct cgccggtagt ctgctgctgc
     2161 gctttcgatt tctgctctaa gtccttcgcc agcgttaaaa gactgttcat agatggctcc
     2221 cttcagtcgg atgtttcgcc ccccgtccgg gtcggcaatg ctgatgctgc tttttgtggt
     2281 gcgcaccacc tcaaaacctg cgctttccag cgcctcagtg acgtcctgac gcgttttcag
     2341 ctccccggat gaggcaaggg caagcaggcc gctcgtaatc gcctgggcgg cttcctgctt
     2401 cgcttccggc agcgcggaag gcgagaccag cacccgccgg ttctccggcg cgttcgggtc
     2461 atgcagcccc agcctgccgt tcactatggt ctgccaggca tcgatgcgcg gacggtcggc
     2521 gcggtcgtaa tacggctgta gccgcttgcc ggtcagaagc tccgtgttcg ggatcaggaa
     2581 gttcagctcc agccgcccct tgtcccggtg ctcaacccac agcacgctgt actggtcttt
     2641 atcgagtccg ggcatcagaa cccgctcgaa actcgccatc agcttttcgc gctggccggg
     2701 cggtaaatcc tgttcggcaa aagacagaac gccggaagtg tactttttgg cgtagggcga
     2761 ggcgtcgata agctcccgga cttcatccgg cttcccctgc agaacgctgg cgccgtcgcg
     2821 ctggcgatct ttacccagca gataatcgac cggcccgccg ccgccgccgc gcccccgagg
     2881 atgaaactta acgatcatca tcagcaccct tttccagtac ggcatgccgc aaccgctcga
     2941 gtccggcatc gatggccatc agcgcggcga caatctgcac gcggtcgtgt ccgcttccac
     3001 caccggcgtt aacctggcgg gcaatctggt taaggttgtt ccccatgccg gcaagctgac
     3061 gaagcagcgc cggcgagatc gacggaagtt tgccggcgcg tgcgggcttc tcgtccaggc
     3121 acgtctgccg catccaggcc gcgagttgct taccctcgca gcgctccagc agacgccggt
     3181 gctcgtcctc agtcacccac atggtgagca ttttgctgcg ttt
//
