LOCUS       CP091129                4357 bp    DNA     circular BCT 02-MAR-2022
DEFINITION  Serratia marcescens strain CM2015_854 plasmid unnamed4, complete
            sequence.
ACCESSION   CP091129
VERSION     CP091129.1
DBLINK      BioProject: PRJNA784350
            BioSample: SAMN23485804
KEYWORDS    .
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Allen,J.L., Doidge,N.P., Bushell,R., Browning,G.F. and Marenda,M.S.
  TITLE     Nosocomial infections caused by chlorhexidine-tolerant Serratia
            marcescens carrying a promiscuous multi-drug resistance IncHI2
            plasmid in a veterinary hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Allen,J.L., Doidge,N.P., Bushell,R., Browning,G.F. and Marenda,M.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2022) Faculty of Veterinary and Agricultural
            Sciences, The University of Melbourne, Cnr Flemington Rd & Park
            Drive, Parkville, Victoria 3010, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 12-MAY-2021
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 6x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/19/2022 10:45:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,875
            CDSs (total)                      :: 5,744
            Genes (coding)                    :: 5,623
            CDSs (with protein)               :: 5,623
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 18
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 47 of 122
            Pseudo Genes (incomplete)         :: 78 of 122
            Pseudo Genes (internal stop)      :: 24 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="CM2015_854"
                     /isolation_source="Wound fluid"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:615"
                     /plasmid="unnamed4"
                     /geo_loc_name="Australia: U-Vet Werribee Animal Hospital"
                     /collection_date="2015-09-28"
                     /note="unpigmented"
     gene            complement(join(3746..4357,1..738))
                     /locus_tag="L2D47_29325"
     CDS             complement(join(3746..4357,1..738))
                     /locus_tag="L2D47_29325"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UMK65533.1"
                     /translation="MAIYSFQCKIVARTKRLNNAVAHAAYINRIDLDNSRDGSKWRYSH
                     SNDDVYKSSVLLPDNASKKFSDPAYLWNEVESKENRSNSRLARSFIIALPNELTPEQNK
                     NLIEDYIKNHMTSKGMIVNYAIHIDDTNNFHAHLMSTTRELDPNGLEFAKKNVIGRTWN
                     DDDFLEGLRKGWADTANTHLARLDHQVRIDHRTLSAQRDEALEQFDMATTAEAKAEALA
                     KAVSLDRPAIGRIWRSKLNTEEGRALREAQQDRRDVQLVVAEETKKSVLSLPQDIVLNN
                     FTPKKIKSKRKTTIEKTRPAPEAVLKTADTGGEIKDLKYSSFSKLMLNIQIMYNEHKKK
                     SIGKKLNKRIKKIEKADKIGGGKGGYFARHLDNDYSVEDNIKSIQEERKEDEYTKLQKF
                     FDDVNKRGIELNNKKEMILKAEQEHKKMLSQQSKYKPKEDNENDRHIKYK"
                     /name="MobA/MobL family protein"
     gene            973..1290
                     /locus_tag="L2D47_29330"
     CDS             973..1290
                     /locus_tag="L2D47_29330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMK65534.1"
                     /translation="MSKAKEKIDNRMAYHLQQLAQLRAKKKEEESKEKVAERKKRASKN
                     ILLGSSICKVLNIDYTQIDEYLPKIIGLLDSKGQHINNPAYLLKGNELLDSWKNDGDRN
                     E"
                     /name="hypothetical protein"
     gene            complement(1442..1738)
                     /locus_tag="L2D47_29335"
     CDS             complement(1442..1738)
                     /locus_tag="L2D47_29335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMK65535.1"
                     /translation="MKDKRKLGFLRGKGSIPDQKQLEEMDQEVSSQFFRDLMESAQEAV
                     EIHQGKRPAARRTLYKVNPVTKSAIERSEAGTDVFTATDASDLFKKLKMDRDN"
                     /name="hypothetical protein"
     gene            2021..2872
                     /locus_tag="L2D47_29340"
     CDS             2021..2872
                     /locus_tag="L2D47_29340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UMK65536.1"
                     /translation="MIIQDNHTIEIFKNQLPVKPYCSNNLENGLSIRNKNKALEMLYLQ
                     ANQPAIQTCLLFDLDEKNAFYKFEDVGLPVPTFITKSPESGRCHYGYMLKAGVCKTQHA
                     RLKPLKYAAAVEAGIARKLASDRGYAGLITKNPLHEHWSPYWSGADLYELDYLADFVEL
                     QTPTKEVKSENYGLGRNVNLFEDLRAYAYKRVLKFKQDLNYEAWERDVLNVAIDLNCRC
                     NALNLLQYNEIKATARSVSRWTWKNFDSATFSKIQSKRAKKPRKTNAKNEMLEILMSKG
                     AL"
                     /name="replication initiation protein"
     gene            2869..3132
                     /locus_tag="L2D47_29345"
     CDS             2869..3132
                     /locus_tag="L2D47_29345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="UMK65531.1"
                     /translation="MIINGKKIKAKEIMEMTGRSERTVRKYFSQSREVYEDEAAERRRK
                     AYELREKGLKWKDIGKELDTSLHGAVALYKRYLKLDLNQQQQ"
                     /name="plasmid replication protein"
     gene            complement(3148..3771)
                     /locus_tag="L2D47_29350"
     CDS             complement(3148..3771)
                     /locus_tag="L2D47_29350"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="UMK65532.1"
                     /translation="MIDTLNINEMAEKYKKILDEGCVILDTETTDLYGEVIELAIINNE
                     GSVLFNHRIKPKEQKITPTAEAIHHISMEMLKDEKELSFYMDELTAIFSKYKNLVIYNA
                     AFDIECLNVSLNANDFQEFKLVFDINIKCAMRDYAYYYNEYNDYFNDYKWQRLETAAKR
                     FDFDFTNITPHEALSDCFMTLHVIESMAKKKTEKVYNLSDFFNR"
                     /name="3'-5' exonuclease"
     Typing          2022..2872
                     /name="rep_cluster_1451"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.988"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3750..4357)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.756"
                     /coverage="100.0"
                     /evalue="1.16e-91"
ORIGIN
        1 tctaccctct tccgtgttca gcttactacg ccaaatccta ccaatagcag gtctatcaag
       61 gcttacagcc ttagccagag cctcagcttt agcctctgca gtggtagcca tatcgaactg
      121 ctcaagagct tcatcacgtt gagctgacag cgttctatgg tcaatcctga cctgatgatc
      181 aagccttgct aaatgagtat tggcggtatc tgcccagcct ttcctcaacc cttcaagaaa
      241 atcatcatca ttccacgttc tgccgataac attctttttt gcgaactcta agccattagg
      301 atctaactcc cttgtagtgc tcatcaaatg ggcatgaaag ttattagtat catctatatg
      361 aatggcatag tttacaatca tacctttcga tgtcatatga tttttaatgt aatcttcaat
      421 aagattttta ttctgttctg gcgttagttc atttggtaag gcaataataa agctgcgagc
      481 caaacgacta tttgagcgat tttcttttga ctcaacttca ttccacaggt aagccggatc
      541 agaaaacttt ttgctggcat tatctggaag aagaacagaa gatttatata catcatcatt
      601 actatgacta taacgccatt ttgaaccatc acggctatta tcaagatcaa tacgattaat
      661 atatgctgca tgagcaacag cattatttaa acgttttgtt cttgctacaa tcttgcattg
      721 aaaactatat atagccatat gagtaaacct ttttttattt tgactttgac cttagcctcg
      781 cagagaccac tacaaacttt agtttgtgta agtgggcatt gctgttttat tttttgcctc
      841 gcatccgctc ggcctaaaat aagcaatgtc gctttactta tatttattag ataacatata
      901 aaaatacttt tgacaaatat ttataaatat attattgaat aaatattaaa ataaataata
      961 ggagtttgat atatgagtaa agcaaaagag aaaatagata atagaatggc ttaccatctt
     1021 caacagcttg cacaactaag agcaaagaaa aaagaagaag agagcaaaga aaaagtagct
     1081 gaaagaaaaa aaagagcgag taaaaatatt ttgcttggct cttctatctg taaagtttta
     1141 aatattgatt acactcaaat agatgaatat ttgccgaaaa taatcggtct tttagactct
     1201 aaaggacagc atataaataa tccagcttat ttattgaaag gcaatgaact tttagatagc
     1261 tggaaaaatg acggcgacag aaacgagtaa aacttgcttt tgctcgtttg ctggcgtcta
     1321 tgcagttagg ttaggaatgg tcataaaatc aacggagtat agtatatatt agtatagtat
     1381 atactgccct ccgggatagc tggccagctt cgtaacttgg tggtttgttt ggtggaggtg
     1441 gtcagttgtc cctgtccatt tttagctttt tgaagagatc agaggcatca gtagctgtaa
     1501 aaacgtctgt accggcttct gagcgttcaa tagctgattt ggtgactggg tttaccttgt
     1561 acagcgttct acgagctgca gggcgtttac cttgatggat ttcgacggct tcctgtgcag
     1621 attccatcag gtcacggaaa aactggctgc tgacttcctg atccatctct tcgagctgct
     1681 tttggtcagg gatagagcct ttaccacgta aaaagccgag cttgcgctta tctttcatat
     1741 cttgcgtatc tccactatcc agcacagcca gatcttactc tctattttat cagccttcgg
     1801 ccttgttggt ttattggaat aggtttccag tagtgccgga aaactgcccc tttacagggc
     1861 tgttatctga tatggttaga cgcacacttg acaaactttt tagatcggtt aaaaccgtta
     1921 cagatttgaa aaataaaaca aaaacaaaaa ccccgcttcc tgtggactgt ttggcgacaa
     1981 acacaagaga gcggggccag ataaaaagga ctgcttaaaa atgataatac aagataacca
     2041 tactatagaa atatttaaaa atcaattacc tgtgaagcct tactgttcca acaatttaga
     2101 aaatggtctt agtatcagga acaaaaacaa agcacttgag atgctttacc ttcaagcaaa
     2161 tcagccagct atacagacgt gcttactgtt cgatctcgat gagaaaaacg ctttctacaa
     2221 gtttgaagat gtagggcttc cagttcctac gttcatcacc aaaagccctg agtctggtag
     2281 gtgtcattat ggctatatgc tcaaagccgg tgtttgtaag acacaacacg caaggctgaa
     2341 gcctttgaag tatgcagctg ctgttgaggc tggcattgct agaaagttag cttctgatcg
     2401 aggatatgct ggactgatta caaaaaatcc actgcatgaa cattggtcac cgtattggtc
     2461 aggtgctgat ctgtatgagt tagattattt ggctgatttt gttgagttac aaacaccaac
     2521 aaaggaagtt aaatcagaga actacgggct gggacgcaat gttaacttgt ttgaagattt
     2581 aagagcttat gcttataagc gagtattgaa gttcaagcag gatttgaact atgaagcgtg
     2641 ggaacgtgat gttttaaatg ttgcaataga tctcaactgc cgatgcaatg cattaaatct
     2701 cttgcaatac aatgagataa aagcaactgc aagatcagtg agccgttgga cttggaagaa
     2761 ctttgatagt gctactttct ccaaaataca atcaaaaagg gcaaaaaaac cacgtaaaac
     2821 aaacgctaaa aatgaaatgt tagaaatatt aatgagcaag ggggcattat gatcattaat
     2881 ggtaaaaaga taaaagcaaa agagattatg gaaatgacag gccgtagtga aagaactgtc
     2941 agaaaatact tttctcaatc tcgtgaagtc tatgaagatg aagcagcaga acgtagaaga
     3001 aaagcgtatg agttgagaga aaaaggctta aaatggaagg acatagggaa agaacttgat
     3061 acgtctttgc atggtgctgt agctctgtat aagcgttatt tgaagttaga tttaaaccaa
     3121 caacaacaat agcaggccgc aagcctgcta tctgttgaag aaatcagaaa gattataaac
     3181 tttctcggtt ttctttttag ccatcgactc tatgacgtgt agcgtcataa aacaatcgct
     3241 taaagcctca tgcggtgtta tgttggtaaa gtcaaaatca aatctttttg ctgctgtttc
     3301 taatctctgc catttgtaat cattaaagta atcgttatat tcattataat aataagcata
     3361 atcacgcatt gcacatttta tattaatatc aaagacgagt ttaaactctt gaaaatcgtt
     3421 cgcgttaagt gatacattta agcattcaat atcaaacgca gcattgtata tgacgagatt
     3481 tttatatttc gaaaaaatag ctgttagttc atccatataa aaagacagct ctttttcatc
     3541 ctttagcatc tccatagata tatgatgaat agcttcagcg gtaggtgtta ttttttgctc
     3601 ttttggtttt attctgtgat tgaacaaaac agaaccttca ttattaatta ttgcgagttc
     3661 tattacttca ccatatagat ctgtcgtttc cgtgtctaaa ataacacatc cttcatcaag
     3721 tattttttta tacttttctg ccatctcatt tatatttaat gtgtctatca ttttcgttgt
     3781 cctcttttgg tttatattta gattgttgtg atagcatctt tttatgctct tgttcagctt
     3841 taagtatcat ttctttttta ttgtttaact ctatacctcg tttatttaca tcatcaaaaa
     3901 acttctgtag ctttgtatat tcatcttctt tgcgttcttc ttgtattgat ttaatattat
     3961 cttcaactga ataatcattg tctaaatgcc tagcaaaata gccaccttta ccaccgccta
     4021 ttttatccgc tttctctatt ttctttattc ttttgtttag cttcttacca atagattttt
     4081 tcttgtgctc attatacatt atttgaatgt ttaacattaa cttactaaaa gaactatatt
     4141 tgagatcttt tatctctccc cctgtatcag cagtttttaa aactgcttct ggggcggggc
     4201 gtgttttttc aatagtagtt tttcttttgg actttatttt tttaggggtg aagttattta
     4261 aaactatatc ctgcggtaaa gacaaaacac tctttttagt ctcttcagca acaacgagct
     4321 gcacatctct tctgtcttgc tgtgcttcac gtaaagc
//
