LOCUS       CP091128                4820 bp    DNA     circular BCT 02-MAR-2022
DEFINITION  Serratia marcescens strain CM2015_854 plasmid unnamed3, complete
            sequence.
ACCESSION   CP091128
VERSION     CP091128.1
DBLINK      BioProject: PRJNA784350
            BioSample: SAMN23485804
KEYWORDS    .
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Allen,J.L., Doidge,N.P., Bushell,R., Browning,G.F. and Marenda,M.S.
  TITLE     Nosocomial infections caused by chlorhexidine-tolerant Serratia
            marcescens carrying a promiscuous multi-drug resistance IncHI2
            plasmid in a veterinary hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Allen,J.L., Doidge,N.P., Bushell,R., Browning,G.F. and Marenda,M.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2022) Faculty of Veterinary and Agricultural
            Sciences, The University of Melbourne, Cnr Flemington Rd & Park
            Drive, Parkville, Victoria 3010, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 12-MAY-2021
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 6x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/19/2022 10:45:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,875
            CDSs (total)                      :: 5,744
            Genes (coding)                    :: 5,623
            CDSs (with protein)               :: 5,623
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 18
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 47 of 122
            Pseudo Genes (incomplete)         :: 78 of 122
            Pseudo Genes (internal stop)      :: 24 of 122
            Pseudo Genes (multiple problems)  :: 22 of 122
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="CM2015_854"
                     /isolation_source="Wound fluid"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:615"
                     /plasmid="unnamed3"
                     /geo_loc_name="Australia: U-Vet Werribee Animal Hospital"
                     /collection_date="2015-09-28"
                     /note="unpigmented"
     gene            63..548
                     /locus_tag="L2D47_29290"
     CDS             63..548
                     /locus_tag="L2D47_29290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UMK65526.1"
                     /translation="MSSLFTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRTELNASA
                     KRISAAISAHEQGVNEAMQSNQRSTLRLVGRTWVTVVTVSVLLIATSGSVLWWQGQQMV
                     ANYTTIEAQKATQAMLSARNNGVQLSTCGDARRTCVKVNPGPGAFGDDSTWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            552..761
                     /locus_tag="L2D47_29295"
     CDS             552..761
                     /locus_tag="L2D47_29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UMK65527.1"
                     /translation="MTELEKQLLSALSQLQQDYSTRLDEWEHAFAEWRTMSGLMQRENA
                     ALNARVSDLSRQIRTLSEQLDRLS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            916..1317
                     /locus_tag="L2D47_29300"
     CDS             916..1317
                     /locus_tag="L2D47_29300"
                     /inference="COORDINATES: protein motif:HMM:NF016255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="UMK65528.1"
                     /translation="MALSEAKKQQQRDARALLEGFWPEAFNFSQPRPLKLGVFEELVAD
                     AKLRGLPFDAAILKAAMKSYTSRYVYQQAVSKIAERTGLDGSPCGEVSAEQKAYAKVQL
                     QRIDAKAKARKKALEADAAAKPVPNEPDA"
                     /name="ProQ/FinO family protein"
     gene            1641..1910
                     /locus_tag="L2D47_29305"
     CDS             1641..1910
                     /locus_tag="L2D47_29305"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMK65529.1"
                     /translation="MKNIIFAVIGVQKTILILSGVSRMIIKPYRRGSDNVQPIIRPDAN
                     PASQPITGVLLGKKSGGGGGCSVSTDAGQKYISHKKTPHSGNSY"
                     /name="hypothetical protein"
     gene            complement(2419..2532)
                     /locus_tag="L2D47_29310"
                     /pseudo=""
     CDS             complement(2419..2532)
                     /locus_tag="L2D47_29310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3086..3721
                     /locus_tag="L2D47_29315"
     CDS             3086..3721
                     /locus_tag="L2D47_29315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMK65525.1"
                     /translation="MITRLIFIAAMAALTAPTYAAAIGNKDCSHPLSASGLHALESSPD
                     YAAVKTEFTKALIAACKEGKSRDDIGFPAADELAGKAADHYVSTHDPKTTDPKAIRNQA
                     GAIYNAWLGGYTGFDLPEDSKKKPARQPMNTAACEQLTKDAIQASIPPNVTITLEQYKK
                     SAGQLGMICMIALQQGYDGKPRNNELLGSLSPEVTEMFNRAYDAGAAQ"
                     /name="hypothetical protein"
     gene            3882..4205
                     /locus_tag="L2D47_29320"
     CDS             3882..4205
                     /locus_tag="L2D47_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UMK65530.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWIRQTCLDEKPARAGKLPSI
                     APALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAALMAIDAGLEQLREAVLAKGPD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(2416..2797)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.362"
                     /coverage="87.0"
                     /evalue="1.5500000000000002e-72"
     Typing          4175..4818
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.698"
                     /coverage="94.0"
                     /evalue="2.99e-120"
ORIGIN
        1 aagcagtatc agcattgccg acccggacgg ggggcaaaac attcgactaa aaggggccct
       61 ctatgagcag tcttttcacg ctggcgaagg acttagagca gaaatcgaaa gcgcagcagc
      121 agagtaccgg cgagatgctg aaagccgcct tcagcgagca cgagaagtct gtaagaacgg
      181 aactgaacgc aagcgcgaag cgaatcagcg ccgccatcag cgcccacgag cagggggtga
      241 acgaggcgat gcagtcgaac cagcgcagca cgctgcggct ggtcgggcgg acgtgggtca
      301 cagtggtgac ggtgtcagtg ctgcttatcg caacaagcgg gagcgtgctg tggtggcagg
      361 ggcagcagat ggtcgcgaat tacacgacca tcgaggcgca gaaggccacg caggcgatgc
      421 tgtcggcgag gaacaacggc gtgcagctgt cgacctgcgg ggacgcgagg cggacgtgcg
      481 tcaaggtgaa cccgggcccg ggcgcgttcg gggacgattc gacctggatg atactggcgg
      541 ggaaataggg aatgacggag ctggaaaagc agttgctgag cgcgttatcg cagctacagc
      601 aggactattc gacaaggctg gacgagtggg agcacgcctt cgcggaatgg cggacgatgt
      661 ctggtcttat gcaacgggag aacgcggcgc tgaacgcgcg cgtctccgac ttgagcaggc
      721 agatacggac tttaagcgag cagttagacc gcttgtccta gaaattgcgt cggtagagca
      781 gcagcgcgcg gcgctgagaa ggcaggcgct ggcgcgcgaa cttgaagacg cgccagcgcc
      841 gcgcccgccg cgaacgtatc acggcccgtc aatgtagggc ggctggccgc atcagtgagt
      901 ttatgaggag aagttatggc gctgtccgaa gccaaaaaac agcagcaacg cgacgcccgg
      961 gccctgctcg aggggttctg gcccgaggcg tttaacttca gccagccgcg cccgttgaaa
     1021 ctgggggttt ttgaggagtt ggtcgccgat gcgaagttgc ggggactgcc tttcgatgcg
     1081 gcgatcctga aggccgccat gaagtcgtac accagccgct acgtctacca gcaagcggtg
     1141 agcaagatag cggagcgcac ggggcttgat ggttcgccgt gcggcgaggt gtcagcggag
     1201 cagaaagcct atgcaaaggt tcagctccag cgcattgatg cgaaagcaaa ggcacgaaaa
     1261 aaagcattag aggccgatgc cgcggcgaaa ccggtgccca acgagccgga tgcatagcta
     1321 tgcacggcct caaaattaac ctgcggcgtt ctgggtggtt ttcggggggt aaggcaggga
     1381 tttatgggcg tcagggcgta aaatcgccct gtgtgccgtt tagacggtgc gctgaacgga
     1441 tgttatggta aattagtctt ctaaacctaa actttgaaaa ccgccgaatc gaaaatcgat
     1501 ttttatcccc agtcgtaaag atttcactta taagatcttt ttattaatct tataaagatc
     1561 ttatagggcg ctcgcagccc agagcctgtt tttcgtgtgg ttagcggtgt ttaatatgtt
     1621 agtcgcgacg attaaaacga atgaagaata ttatcttcgc cgtgataggc gtacagaaaa
     1681 ccattttaat tttaagcgga gttagccgaa tgattattaa accttatcgc cgtgggagtg
     1741 ataacgttca gccaattatc cgcccggacg ctaacccagc aagccagcct ataaccggcg
     1801 tattactcgg aaagaaatca ggcggcggcg ggggctgttc tgtatcgacg gatgccggac
     1861 agaaatatat atctcacaag aaaacgccgc attctggtaa ctcgtattaa gttgggattt
     1921 acgtacactt tgagttaggg tttacatacg attctctccc cgctatcccg cgtcggcacg
     1981 acgggtttaa acaccttgga ctgcccgttg ccgtggtagg tcttttatcc tgtgagttct
     2041 gcaatgtcgt agccctgcca cagctacaga atccacgtag cgttaccggc tggctgtact
     2101 gtgcctgttg tgcctgctct agtgggtata taaaaaccaa gagggcgtag cgcccgtcgt
     2161 ctaggtcgtt tattgtttgt cttgctctgc aaagggtttg attgtgtggg cctgggttaa
     2221 atcgttgcgt tgacagttac gtgtgacgag atgtaatctc tgaaacgaaa aaaccgccca
     2281 gcaaggcggt tttttcgaag gttcgcagag cagcaactct ttgaaccaag gtaactggct
     2341 tggaggagcg cagtcaccaa atctgtcctt tcagtttagc cttaaacggc ccataacttc
     2401 aagactcact cctctaaatc agttaccagt ggctaccgcc aatggcgatt cgtcgtgtct
     2461 gtaagggttg gactcaagtt gatagttacc ttacttggcg tggtagtcgg actgaacggg
     2521 gggttcgtgc atacagtcca gcttggagcg aaccgtctac gtggagttga gtgtcaggcg
     2581 tggtatgaga aaccgcagcc ataattgcgg aagacaccga caacccataa gacgggaaca
     2641 caggagcgca cgagggagcc gccagaggga aacgtctggc atctttaagc tgtgtcgggt
     2701 ttcgccacca ctgatttgag cgccggaaat catccgattt ctgtgagtct cgtcaggggg
     2761 gcggagccta tggtaaaacg ggttccccgc gccctttgct ctgacccttc ttgcctgccg
     2821 cacctgtcct acacttcctc acgatgaccc gcaacgcccg ccgcagcctc atgaccgaac
     2881 gcagtgagcg aatgagcgag gaggcgtcat atcatctcca ctgccattta cccgtgccac
     2941 tcctctgccg gtctgattac gccttaatcc catgatcctt ttgacctttc aaccactccg
     3001 atttagcata ttaagccagt atacactccg ctagcgctac gtgactggtt cagggctgcg
     3061 ccccgaaacc tgcccaaagg aaaaaatgat tacacgattg atatttattg ctgccatggc
     3121 ggctctcacg gcacctactt atgctgccgc tatagggaat aaagattgct cccatcccct
     3181 cagcgcctct ggtctccatg cgcttgaaag ttcacctgac tatgctgctg tgaaaaccga
     3241 gtttaccaag gctctcattg cagcctgtaa agagggaaaa agccgtgatg acataggctt
     3301 tcctgccgct gacgaattgg cagggaaggc tgctgatcac tacgtttcga cgcatgaccc
     3361 taaaaccaca gaccctaaag ctatccgtaa tcaggccggg gctatttata acgcctggtt
     3421 agggggctat acaggttttg acctacctga agattcaaaa aagaaacccg cgcgtcagcc
     3481 catgaatact gctgcatgcg aacaactgac gaaagatgcg atacaggcca gcattccgcc
     3541 aaacgtcact atcacgttgg aacagtacaa aaagagtgct ggccaactcg gtatgatttg
     3601 catgatcgcc ttgcagcagg gttacgacgg caagccacga aacaatgaat tacttggctc
     3661 gttaagtcct gaagtcactg aaatgtttaa ccgtgcatat gatgcaggag cggcacagta
     3721 ggttttttgg cggcaatccc gactcaatat gcatgggtat cgtattaaca aggtttgata
     3781 accgcatcat ttaatcttgc ctaaccatgt gcagaccgga aaaagttccc cgtctgtccc
     3841 ggtgttctca ggaggcccgc ttgtcagaga aacgcaacaa aatgctcacg atgtgggtga
     3901 ctgaggacga gcaccgtcga ctgctggagc gttgcgacgg taaacagctc gcggcatgga
     3961 ttcggcaaac gtgccttgat gaaaagccgg cgcgtgccgg caaacttcct tcgatcgcgc
     4021 cggcgctact gcgccaactg gcgggcatgg gcaataacct gaaccagatc gcccggcagg
     4081 ttaacgccgg gggcggtacc gggcacgacc gggtgcaggt cgtggcggca ctgatggcca
     4141 tcgatgccgg actggagcag ctccgcgagg cggtgctggc aaaggggccg gacgatgatc
     4201 gttaaatttc acccccgggg gcgcgggggc ggcgccgggc cggtggacta cctgctgggg
     4261 aaagaccggc agcgcgaggg cgccagcgtt ctgcagggcc gcgcagagtc ggtgcgtgaa
     4321 cttatcgacg cgtcgcccta cgccaagaag tacacctccg gcgtgctgtc gtttgcggag
     4381 gccgacctgc cgcccggtca gcgcgaaaaa ttgatggcaa gcttcgagcg ggttctgatg
     4441 cccggcctcg aaaaagacca gtacagcgtg ctgtgggtgg agcacgccga caaggggcgg
     4501 ctggagctga atttccttat cccgaatacc gacctgttga cgggaaggcg gctccagccc
     4561 tattacgacc gggccgaccg cccgcgcatc gatgcctggc aaacgctggt caacggcagg
     4621 ctggggcttc acgacccgaa cgcgccggag aaccgccggg cgctggtcac gccgtcggcc
     4681 ctgcccaaaa cgaagcagga agccgctcag gccattacgc gcgggctgct cgcgctggcc
     4741 tcctccggtg agcttgcaac gcggcaggac gtcaccgagg cgctgacgcg gtcagggttt
     4801 gaggtggtac gcaccacgaa
//
