LOCUS       CP090292               12954 bp    DNA     circular BCT 10-JAN-2022
DEFINITION  Escherichia coli strain E-T654 plasmid punnamed3, complete sequence.
ACCESSION   CP090292
VERSION     CP090292.1
DBLINK      BioProject: PRJNA792053
            BioSample: SAMN24394421
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12954)
  AUTHORS   Chen,J., Liu,C., Zeng,Y., Zhang,R. and Lu,J.
  TITLE     Phenotypic and Genetic Characterization of Carbapenem-Resistant
            Escherichia coli from ICU Patients in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12954)
  AUTHORS   Chen,J., Liu,C., Zeng,Y., Zhang,R. and Lu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-DEC-2021) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine, 88
            Jiefang Rd, Hangzhou, Zhejiang Province 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq 6000; Nanopore PromethION
            48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2022 13:03:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,843
            CDSs (total)                      :: 4,725
            Genes (coding)                    :: 4,487
            CDSs (with protein)               :: 4,487
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 64 of 238
            Pseudo Genes (incomplete)         :: 159 of 238
            Pseudo Genes (internal stop)      :: 46 of 238
            Pseudo Genes (multiple problems)  :: 30 of 238
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E-T654"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="punnamed3"
                     /geo_loc_name="China"
                     /collection_date="2020"
     gene            complement(634..1047)
                     /locus_tag="LZT31_00595"
     CDS             complement(634..1047)
                     /locus_tag="LZT31_00595"
                     /inference="COORDINATES: protein motif:HMM:NF036929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="UIM30727.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            complement(1205..1657)
                     /locus_tag="LZT31_00600"
                     /pseudo=""
     CDS             complement(1205..1657)
                     /locus_tag="LZT31_00600"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            1995..2279
                     /locus_tag="LZT31_00605"
     CDS             1995..2279
                     /locus_tag="LZT31_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="UIM30719.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            complement(2949..3845)
                     /gene="estX"
                     /locus_tag="LZT31_00610"
     CDS             complement(2949..3845)
                     /gene="estX"
                     /locus_tag="LZT31_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold putative hydrolase EstX"
                     /protein_id="UIM30720.1"
                     /translation="MNKMLYVKLEGNFSMKEKVVVDKAISLHTESFGDPAHEPIILIMG
                     AMASAVWWPDEFCSQLAKMGRYVIRYDHRDTGKSTSYEPGQAPYSVEELADDVVRVIDG
                     YGLEAAHLVGMSLGGFLSQLVALKYPKRVKSLTLIASERLADADPDMPAFDPAIIEYHQ
                     RAESLDWSDRDAVVAYQVGAWRIISGTAHAFDAEKIQNIAELDFDRTPNILTTFNHTTS
                     GGGERSLERWLGRLNEIAVPTLIIHGTEDPVLPYVHGLALKDAIRGSKMLTLEGTGHEL
                     HHEDWPRIIQAIKGQTS"
                     /name="estX"
     gene            complement(3955..4806)
                     /locus_tag="LZT31_00615"
     CDS             complement(3955..4806)
                     /locus_tag="LZT31_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="UIM30721.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="replication protein"
     gene            complement(4793..5632)
                     /locus_tag="LZT31_00620"
     CDS             complement(4793..5632)
                     /locus_tag="LZT31_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="UIM30722.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(5662..5868)
                     /locus_tag="LZT31_00625"
     CDS             complement(5662..5868)
                     /locus_tag="LZT31_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIM30723.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="hypothetical protein"
     gene            complement(5870..6082)
                     /locus_tag="LZT31_00630"
     CDS             complement(5870..6082)
                     /locus_tag="LZT31_00630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIM30724.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            complement(6143..7012)
                     /locus_tag="LZT31_00635"
     CDS             complement(6143..7012)
                     /locus_tag="LZT31_00635"
                     /inference="COORDINATES: protein motif:HMM:NF028103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family DNA primase"
                     /protein_id="UIM30725.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="RepB family DNA primase"
     gene            complement(7111..7524)
                     /locus_tag="LZT31_00640"
     CDS             complement(7111..7524)
                     /locus_tag="LZT31_00640"
                     /inference="COORDINATES: protein motif:HMM:NF036929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="UIM30726.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            complement(7682..8134)
                     /locus_tag="LZT31_00645"
                     /pseudo=""
     CDS             complement(7682..8134)
                     /locus_tag="LZT31_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            8472..8756
                     /locus_tag="LZT31_00650"
     CDS             8472..8756
                     /locus_tag="LZT31_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="UIM30713.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            complement(9426..10322)
                     /gene="estX"
                     /locus_tag="LZT31_00655"
     CDS             complement(9426..10322)
                     /gene="estX"
                     /locus_tag="LZT31_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold putative hydrolase EstX"
                     /protein_id="UIM30714.1"
                     /translation="MNKMLYVKLEGNFSMKEKVVVDKAISLHTESFGDPAHEPIILIMG
                     AMASAVWWPDEFCSQLAKMGRYVIRYDHRDTGKSTSYEPGQAPYSVEELADDVVRVIDG
                     YGLEAAHLVGMSLGGFLSQLVALKYPKRVKSLTLIASERLADADPDMPAFDPAIIEYHQ
                     RAESLDWSDRDAVVAYQVGAWRIISGTAHAFDAEKIQNIAELDFDRTPNILTTFNHTTS
                     GGGERSLERWLGRLNEIAVPTLIIHGTEDPVLPYVHGLALKDAIRGSKMLTLEGTGHEL
                     HHEDWPRIIQAIKGQTS"
                     /name="estX"
     gene            complement(10432..11283)
                     /locus_tag="LZT31_00660"
     CDS             complement(10432..11283)
                     /locus_tag="LZT31_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="UIM30715.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="replication protein"
     gene            complement(11270..12109)
                     /locus_tag="LZT31_00665"
     CDS             complement(11270..12109)
                     /locus_tag="LZT31_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="UIM30716.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(12139..12345)
                     /locus_tag="LZT31_00670"
     CDS             complement(12139..12345)
                     /locus_tag="LZT31_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIM30717.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="hypothetical protein"
     gene            complement(12347..12559)
                     /locus_tag="LZT31_00675"
     CDS             complement(12347..12559)
                     /locus_tag="LZT31_00675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIM30718.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     Typing          complement(1885..2041)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.38e-77"
     Typing          complement(8362..8518)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.38e-77"
     Typing          complement(4794..5615)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(11271..12092)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(3..1795)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6147..8272)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctgccgctc gggcagttcg aggctggcca gcctgcgggc cttctcctgc tgccgctggg
       61 cctgctcgat ctgctggcca gcctgctgca ccagcgccgg gccagcggtg gcggtcttgc
      121 ccttggattc acgcagcagc acccacggct gataaccggc gcgggtggtg tgcttgtcct
      181 tgcggttggt gaagcccgcc aagcggccat agtggcggct gtcggcgctg gccgggtcgg
      241 cgtcgtactc gctggccagc gtccgggcaa tctgcccccg aagttcaccg cctgcggcgt
      301 cggccacctt gacccatgcc tgatagttct tcgggctggt ttccactacc agggcaggct
      361 cccggccctc ggctttcatg tcatccaggt caaactcgct gaggtcgtcc accagcacca
      421 gaccatgccg ctcctgctcg gcgggcctga tatacacgtc attgccctgg gcattcatcc
      481 gcttgagcca tggcgtgttc tggagcactt cggcggctga ccattcccgg ttcatcatct
      541 ggccggtggt ggcgtccctg acgccgatat cgaagcgctc acagcccatg gccttgagct
      601 gtcggcctat ggcctgcaaa gtcctgtcgt tcttcatcgg gccaccaagc gcagccagat
      661 cgagccgtcc tcggttgtca gtggcgtcag gtcgagcaag agcaacgatg cgatcagcag
      721 caccaccgta ggcatcatgg aagccagcat cacggttagc catagcttcc agtgccaccc
      781 ccgcgacgcg ctccgggcgc tctgcgcggc gctgctcacc tcggcggcta cctcccgcaa
      841 ctctttggcc agctccaccc atgccgcccc tgtctggcgc tgggctttca gccactccgc
      901 cgcctgcgcc tcgctggcct gctgggtctg gctcatgacc tgccgggctt cgtcggccag
      961 tgtcgccatg ctctgggcca gcggttcgat ctgctccgct aactcgttga tgcctctgga
     1021 tttcttcact ctgtcgattg cgttcatggt ctattgcctc ccggtattcc tgtaagtcga
     1081 tgatctgggc gttggcggtg tcgatgttca gggccacgtc tgcccggtcg gtgcggatgc
     1141 cccggccttc catctccacc acgttcggcc ccaggtgaac accgggcagg cgctcgatgc
     1201 cctgcgcctc aagtgttctg tggtcaatgc gggcgtcgtg gccagcccgc tctaatgccc
     1261 ggttggcatg gtcggcccat gcctcgcggg tctgctcaag ccatgccttg ggcttgagcg
     1321 cttcggtctt ctgtgccccg cccttctccg gggtcttgcc gttgtaccgc ttgaaccact
     1381 gagcggcggg ccgctcgatg ccgtcattga tccgctcgga gatcatcagg tggcagtgcg
     1441 ggttctcgcc gccaccggca tggatggcca gcgtatacgg caggcgctcg gcaccagtca
     1501 ggtgctgggc gaactcggac gccagcgcct tctgctggtc gagggtcagc tcgaccggca
     1561 gggcaaattc gacctccttg aacagccgcc cattggcgcg ttcatacagg tcggcagcat
     1621 cccagtagtc ggcgggccgc tcgacgaact ccggcatgtg cccggattcg gcgtgcaaga
     1681 cttcatccat gtcgcgggca tacttgcctt cgcgctggat gtagtcggcc ttggccctgg
     1741 ccgattggcc gcccgacctg ctgccggttt tcgccgtaag gtgataaatc gccatgctgc
     1801 ctcgctgttg cttttgcttt tcggctccat gcaatggccc tcggagagcg caccgcccga
     1861 agggtggccg ttaggccagt ttctcgaaga gaaaccggta agtgcgccct ccccctacaa
     1921 agtagggtcg ggattgccgc cgctgtgcct ccatgatagc ctacgagaca gcacattaac
     1981 aatggggtgt caagatggtt aaggggagca acaaggcggc ggatcggctg gccaagctcg
     2041 aagaacaacg agcgcgaatc aatgccgaaa ttcagcgggt gcgggcaagg gaacagcagc
     2101 aagagcgcaa gaacgaaaca aggcgcaagg tgctggtggg ggccatgatt ttggccaagg
     2161 tgaacagcag cgagtggccg gaggatcggc tcatggcggc aatggatgcg taccttgaac
     2221 gcgaccacga ccgcgccttg ttcggtctgc cgccacgcca gaaggatgag ccgggctgaa
     2281 tgatcgaccg agacaggccc tgcggggctg cacacgcgcc cccacccttc gggtaggggg
     2341 aaaggccgct aaagcggcta aaagcgctcc agcgtatttc tgcggggttt ggtgtggggt
     2401 ttagcgggct ttgcccgcct ttccccctgc cgcgcagcgg tggggcggtg tgtagcctag
     2461 cgcagcgaat agaccagcta tccggcctct ggccgggcat attgggcaag ggcagcagcg
     2521 ccccacaagg gcgctgataa ccgcgcctag tggattattc ttagataatc atggatggat
     2581 ttttccaaca ccccgccagc ccccgcccct gctgggtttg caggtttggg ggcgtgacag
     2641 ttattgcagg ggttcgtgac agttattgca ggggggcgtg acagttattg caggggttcg
     2701 tgacagttag tacgggagtg acgggcactg gctggcaatg tctagcaacg gcaggcattt
     2761 cggctgaggg taaaagaact ttccgctaag cgatagactg tatgtaaaca cagtattgca
     2821 aggacgcgga acatgcctca gcgatcaccg cttccctcat gatgtttaac gccttgctca
     2881 ccggaaaatt gcgagcgcag cgagtaattt ttacggtgca gcaacttgtt gtacggtgca
     2941 accgatagct atgacgtttg ccccttaatc gcctggataa tcctcggcca gtcttcatga
     3001 tgcaactcat gtcccgtgcc ttcgagtgtc agcatttttg aaccacgaat cgcatctttc
     3061 agtgccaacc catgcacata aggaagtaca ggatcctccg tgccgtgaat gatcaaagtt
     3121 ggtacagcta tctcatttaa tctcccgagc catctctcga gcgatctctc gccaccacct
     3181 gaagtagtgt ggttgaatgt tgtcaggata ttcggagtgc gatcaaaatc taactcagcg
     3241 atattttgta tcttctcagc gtcaaaagca tgcgcagtac ctgagatgat tcgccacgct
     3301 ccgacctgat acgcgacgac agcatctcta tcagaccaat ccagcgattc cgcccgttgg
     3361 tgatactcaa tgatggcagg atcaaaagcg ggcatatcag gatctgcatc tgcaagccgt
     3421 tctgaagcaa tcagcgtcaa gctcttcaca cgtttcggat acttgagagc tacaagctgg
     3481 gaaagaaatc cccccaaaga catgccgact aaatgagcag cttccagacc ataaccatca
     3541 atgacgcgaa ccacatcatc tgctaattct tcaacggaat atggagcttg acctggctca
     3601 tagcttgttg atttcccggt atcacggtgg tcgtaccgga tcacatagcg acccattttg
     3661 gctagttggg agcaaaactc atcaggccac cacaccgcag acgccattgc ccccatgatc
     3721 aggataatgg gttcatgggc cgggtcgccg aatgattcgg tatggagtga aatcgcttta
     3781 tcaacaacta ccttttcttt catactaaaa ttgccttcga gttttacgta caacatttta
     3841 ttcataggca tgctcatttt gccagttttg gctgcctgtt aattgcgctc aaacatcccc
     3901 aaccaattgt tttataaaga taaaaatgtc ttgtttacaa tagagtgggg ggggtcagcc
     3961 tgccgccttg ggccgggtga tgtcgtactt gcccgccgcg aactcggtta ccgtccagcc
     4021 cagcgcgacc agctccggca acgcctcgcg cacccgctgg cggcgcttgc gcatggtcga
     4081 accactggcc tctgacggcc agacatagcc gcacaaggta tctatggaag ccttgccggt
     4141 tttgccgggg tcgatccagc cacacagccg ctggtgcagc aggcgggcgg tttcgctgtc
     4201 cagcgcccgc acctcgtcca tgctgatgcg cacatgctgg ccgccaccca tgacggcctg
     4261 cgcgatcaag gggttcaggg ccacgtacag gcgcccgtcc gcctcgtcgc tggcgtactc
     4321 cgacagcagc cgaaacccct gccgcttgcg gccattctgg gcgatgatgg ataccttcca
     4381 aaggcgctcg atgcagtcct gtatgtgctt gagcgcccca ccactatcga cctctgcccc
     4441 gatttccttt gccagcgccc gatagctacc tttgaccacc atggcatcag cggtgacggc
     4501 ctcccacttg ggttccagga acagccggag ctgccgtccg ccttcggtct tgggttccgg
     4561 gccaagcact aggccattag gcccagccat ggccaccagc ccttgcagga tgcgcagatc
     4621 atcagcgccc agcggctccg ggccgctgaa ctcgatccgc ttgccgtcgc cgtagtcata
     4681 cgtcacgtcc agcttgctgc gcttgcgctc gccccgcttg agggcacgga acaggccggg
     4741 ggccagacag tgcgccgggt cgtgccggac gtggctgagg ctgtgcttgt tcttaggctt
     4801 caccacgggg cacccccttg ctcttgcgct gcctctccag cacggcgggc ttgagcaccc
     4861 cgccgtcatg ccgcctgaac caccgatcag cgaacggtgc gccatagttg gccttgctca
     4921 caccgaagcg gacgaagaac cggcgctggt cgtcgtccac accccattcc tcggcctcgg
     4981 cgctggtcat gctcgacagg taggactgcc agcggatgtt atcgaccagt accgagctgc
     5041 cccggctggc ctgctgctgg tcgcctgcgc ccatcatggc cgcgcccttg ctggcatggt
     5101 gcaggaacac gatagagcac ccggtatcgg cggcgatggc ctccatgcga ccgatgacct
     5161 gggccatggg gccgctggcg ttttcttcct cgatgtggaa ccggcgcagc gtgtccagca
     5221 ccatcaggcg gcggccctcg gcggcgcgct tgaggccgtc gaaccactcc ggggccatga
     5281 tgttgggcag gctgccgatc agcggctgga tcagcaggcc gtcagccacg gcttgccgtt
     5341 cctcggcgct gaggtgcgcc ccaagggcgt gcaggcggtg atgaatggcg gtgggcgggt
     5401 cttcggcggg caggtagatc accgggccgg tgggcagttc gcccacctcc agcagatccg
     5461 gcccgcctgc aatctgtgcg gccagttgca gggccagcat ggatttaccg gcaccaccgg
     5521 gcgacaccag cgccccgacc gtaccggcca ccatgttggg caaaacgtag tccagcggtg
     5581 gcggcgctgc tgcgaacgcc tccagaatat tgataggctt atgggtagcc attgattgcc
     5641 tcctttgcag gcagttggtg gttaggcgct ggcggggtca ctacccccgc cctgcgccgc
     5701 tctgagttct tccaggcact cgcgcagcgc ctcgtattcg tcgtcggtca gccagaactt
     5761 gcgctgacgc atccctttgg ccttcatgcg ctcggcatat cgcgcttggc gtacagcgtc
     5821 agggctggcc agcaggtcgc cggtctgctt gtccttttgg tctttcatat cagtcaccga
     5881 gaaacttgcc ggggccgaaa ggcttgtctt cgcggaacaa ggacaaggtg cagccgtcaa
     5941 ggttaaggct ggccatatca gcgactgaaa agcggccagc ctcggccttg tttgacgtat
     6001 aaccaaagcc accgggcaac caatagccct tgtcactttt gatcaggtag accgaccctg
     6061 aagcgctttt ttcgtattcc ataaaacccc cttctgtgcg tgagtactca tagtataaca
     6121 ggcgtgagta ccaacgcaag cactacatgc tgaaatctgg cccgcccctg tccatgcctc
     6181 gctggcgggg tgccggtgcc cgtgccagct cggcccgcgc aagctggacg ctgggcagac
     6241 ccatgacctt gctgacggtg cgctcgatgt aatccgcttc gtggccgggc ttgcgctctg
     6301 ccagcgctgg gctggcctcg gccatggcct tgccgatttc ctcggcactg cggccccggc
     6361 tggccagctt ctgcgcggcg ataaagtcgc acttgctgag gtcatcaccg aagcgcttga
     6421 ccagcccggc catctcgctg cggtactcgt ccagcgccgt gcgccggtgg cggctaagct
     6481 gccgctcggg cagttcgagg ctggccagcc tgcgggcctt ctcctgctgc cgctgggcct
     6541 gctcgatctg ctggccagcc tgctgcacca gcgccgggcc agcggtggcg gtcttgccct
     6601 tggattcacg cagcagcacc cacggctgat aaccggcgcg ggtggtgtgc ttgtccttgc
     6661 ggttggtgaa gcccgccaag cggccatagt ggcggctgtc ggcgctggcc gggtcggcgt
     6721 cgtactcgct ggccagcgtc cgggcaatct gcccccgaag ttcaccgcct gcggcgtcgg
     6781 ccaccttgac ccatgcctga tagttcttcg ggctggtttc cactaccagg gcaggctccc
     6841 ggccctcggc tttcatgtca tccaggtcaa actcgctgag gtcgtccacc agcaccagac
     6901 catgccgctc ctgctcggcg ggcctgatat acacgtcatt gccctgggca ttcatccgct
     6961 tgagccatgg cgtgttctgg agcacttcgg cggctgacca ttcccggttc atcatctggc
     7021 cggtggtggc gtccctgacg ccgatatcga agcgctcaca gcccatggcc ttgagctgtc
     7081 ggcctatggc ctgcaaagtc ctgtcgttct tcatcgggcc accaagcgca gccagatcga
     7141 gccgtcctcg gttgtcagtg gcgtcaggtc gagcaagagc aacgatgcga tcagcagcac
     7201 caccgtaggc atcatggaag ccagcatcac ggttagccat agcttccagt gccacccccg
     7261 cgacgcgctc cgggcgctct gcgcggcgct gctcacctcg gcggctacct cccgcaactc
     7321 tttggccagc tccacccatg ccgcccctgt ctggcgctgg gctttcagcc actccgccgc
     7381 ctgcgcctcg ctggcctgct gggtctggct catgacctgc cgggcttcgt cggccagtgt
     7441 cgccatgctc tgggccagcg gttcgatctg ctccgctaac tcgttgatgc ctctggattt
     7501 cttcactctg tcgattgcgt tcatggtcta ttgcctcccg gtattcctgt aagtcgatga
     7561 tctgggcgtt ggcggtgtcg atgttcaggg ccacgtctgc ccggtcggtg cggatgcccc
     7621 ggccttccat ctccaccacg ttcggcccca ggtgaacacc gggcaggcgc tcgatgccct
     7681 gcgcctcaag tgttctgtgg tcaatgcggg cgtcgtggcc agcccgctct aatgcccggt
     7741 tggcatggtc ggcccatgcc tcgcgggtct gctcaagcca tgccttgggc ttgagcgctt
     7801 cggtcttctg tgccccgccc ttctccgggg tcttgccgtt gtaccgcttg aaccactgag
     7861 cggcgggccg ctcgatgccg tcattgatcc gctcggagat catcaggtgg cagtgcgggt
     7921 tctcgccgcc accggcatgg atggccagcg tatacggcag gcgctcggca ccagtcaggt
     7981 gctgggcgaa ctcggacgcc agcgccttct gctggtcgag ggtcagctcg accggcaggg
     8041 caaattcgac ctccttgaac agccgcccat tggcgcgttc atacaggtcg gcagcatccc
     8101 agtagtcggc gggccgctcg acgaactccg gcatgtgccc ggattcggcg tgcaagactt
     8161 catccatgtc gcgggcatac ttgccttcgc gctggatgta gtcggccttg gccctggccg
     8221 attggccgcc cgacctgctg ccggttttcg ccgtaaggtg ataaatcgcc atgctgcctc
     8281 gctgttgctt ttgcttttcg gctccatgca atggccctcg gagagcgcac cgcccgaagg
     8341 gtggccgtta ggccagtttc tcgaagagaa accggtaagt gcgccctccc cctacaaagt
     8401 agggtcggga ttgccgccgc tgtgcctcca tgatagccta cgagacagca cattaacaat
     8461 ggggtgtcaa gatggttaag gggagcaaca aggcggcgga tcggctggcc aagctcgaag
     8521 aacaacgagc gcgaatcaat gccgaaattc agcgggtgcg ggcaagggaa cagcagcaag
     8581 agcgcaagaa cgaaacaagg cgcaaggtgc tggtgggggc catgattttg gccaaggtga
     8641 acagcagcga gtggccggag gatcggctca tggcggcaat ggatgcgtac cttgaacgcg
     8701 accacgaccg cgccttgttc ggtctgccgc cacgccagaa ggatgagccg ggctgaatga
     8761 tcgaccgaga caggccctgc ggggctgcac acgcgccccc acccttcggg tagggggaaa
     8821 ggccgctaaa gcggctaaaa gcgctccagc gtatttctgc ggggtttggt gtggggttta
     8881 gcgggctttg cccgcctttc cccctgccgc gcagcggtgg ggcggtgtgt agcctagcgc
     8941 agcgaataga ccagctatcc ggcctctggc cgggcatatt gggcaagggc agcagcgccc
     9001 cacaagggcg ctgataaccg cgcctagtgg attattctta gataatcatg gatggatttt
     9061 tccaacaccc cgccagcccc cgcccctgct gggtttgcag gtttgggggc gtgacagtta
     9121 ttgcaggggt tcgtgacagt tattgcaggg gggcgtgaca gttattgcag gggttcgtga
     9181 cagttagtac gggagtgacg ggcactggct ggcaatgtct agcaacggca ggcatttcgg
     9241 ctgagggtaa aagaactttc cgctaagcga tagactgtat gtaaacacag tattgcaagg
     9301 acgcggaaca tgcctcagcg atcaccgctt ccctcatgat gtttaacgcc ttgctcaccg
     9361 gaaaattgcg agcgcagcga gtaattttta cggtgcagca acttgttgta cggtgcaacc
     9421 gatagctatg acgtttgccc cttaatcgcc tggataatcc tcggccagtc ttcatgatgc
     9481 aactcatgtc ccgtgccttc gagtgtcagc atttttgaac cacgaatcgc atctttcagt
     9541 gccaacccat gcacataagg aagtacagga tcctccgtgc cgtgaatgat caaagttggt
     9601 acagctatct catttaatct cccgagccat ctctcgagcg atctctcgcc accacctgaa
     9661 gtagtgtggt tgaatgttgt caggatattc ggagtgcgat caaaatctaa ctcagcgata
     9721 ttttgtatct tctcagcgtc aaaagcatgc gcagtacctg agatgattcg ccacgctccg
     9781 acctgatacg cgacgacagc atctctatca gaccaatcca gcgattccgc ccgttggtga
     9841 tactcaatga tggcaggatc aaaagcgggc atatcaggat ctgcatctgc aagccgttct
     9901 gaagcaatca gcgtcaagct cttcacacgt ttcggatact tgagagctac aagctgggaa
     9961 agaaatcccc ccaaagacat gccgactaaa tgagcagctt ccagaccata accatcaatg
    10021 acgcgaacca catcatctgc taattcttca acggaatatg gagcttgacc tggctcatag
    10081 cttgttgatt tcccggtatc acggtggtcg taccggatca catagcgacc cattttggct
    10141 agttgggagc aaaactcatc aggccaccac accgcagacg ccattgcccc catgatcagg
    10201 ataatgggtt catgggccgg gtcgccgaat gattcggtat ggagtgaaat cgctttatca
    10261 acaactacct tttctttcat actaaaattg ccttcgagtt ttacgtacaa cattttattc
    10321 ataggcatgc tcattttgcc agttttggct gcctgttaat tgcgctcaaa catccccaac
    10381 caattgtttt ataaagataa aaatgtcttg tttacaatag agtggggggg gtcagcctgc
    10441 cgccttgggc cgggtgatgt cgtacttgcc cgccgcgaac tcggttaccg tccagcccag
    10501 cgcgaccagc tccggcaacg cctcgcgcac ccgctggcgg cgcttgcgca tggtcgaacc
    10561 actggcctct gacggccaga catagccgca caaggtatct atggaagcct tgccggtttt
    10621 gccggggtcg atccagccac acagccgctg gtgcagcagg cgggcggttt cgctgtccag
    10681 cgcccgcacc tcgtccatgc tgatgcgcac atgctggccg ccacccatga cggcctgcgc
    10741 gatcaagggg ttcagggcca cgtacaggcg cccgtccgcc tcgtcgctgg cgtactccga
    10801 cagcagccga aacccctgcc gcttgcggcc attctgggcg atgatggata ccttccaaag
    10861 gcgctcgatg cagtcctgta tgtgcttgag cgccccacca ctatcgacct ctgccccgat
    10921 ttcctttgcc agcgcccgat agctaccttt gaccaccatg gcatcagcgg tgacggcctc
    10981 ccacttgggt tccaggaaca gccggagctg ccgtccgcct tcggtcttgg gttccgggcc
    11041 aagcactagg ccattaggcc cagccatggc caccagccct tgcaggatgc gcagatcatc
    11101 agcgcccagc ggctccgggc cgctgaactc gatccgcttg ccgtcgccgt agtcatacgt
    11161 cacgtccagc ttgctgcgct tgcgctcgcc ccgcttgagg gcacggaaca ggccgggggc
    11221 cagacagtgc gccgggtcgt gccggacgtg gctgaggctg tgcttgttct taggcttcac
    11281 cacggggcac ccccttgctc ttgcgctgcc tctccagcac ggcgggcttg agcaccccgc
    11341 cgtcatgccg cctgaaccac cgatcagcga acggtgcgcc atagttggcc ttgctcacac
    11401 cgaagcggac gaagaaccgg cgctggtcgt cgtccacacc ccattcctcg gcctcggcgc
    11461 tggtcatgct cgacaggtag gactgccagc ggatgttatc gaccagtacc gagctgcccc
    11521 ggctggcctg ctgctggtcg cctgcgccca tcatggccgc gcccttgctg gcatggtgca
    11581 ggaacacgat agagcacccg gtatcggcgg cgatggcctc catgcgaccg atgacctggg
    11641 ccatggggcc gctggcgttt tcttcctcga tgtggaaccg gcgcagcgtg tccagcacca
    11701 tcaggcggcg gccctcggcg gcgcgcttga ggccgtcgaa ccactccggg gccatgatgt
    11761 tgggcaggct gccgatcagc ggctggatca gcaggccgtc agccacggct tgccgttcct
    11821 cggcgctgag gtgcgcccca agggcgtgca ggcggtgatg aatggcggtg ggcgggtctt
    11881 cggcgggcag gtagatcacc gggccggtgg gcagttcgcc cacctccagc agatccggcc
    11941 cgcctgcaat ctgtgcggcc agttgcaggg ccagcatgga tttaccggca ccaccgggcg
    12001 acaccagcgc cccgaccgta ccggccacca tgttgggcaa aacgtagtcc agcggtggcg
    12061 gcgctgctgc gaacgcctcc agaatattga taggcttatg ggtagccatt gattgcctcc
    12121 tttgcaggca gttggtggtt aggcgctggc ggggtcacta cccccgccct gcgccgctct
    12181 gagttcttcc aggcactcgc gcagcgcctc gtattcgtcg tcggtcagcc agaacttgcg
    12241 ctgacgcatc cctttggcct tcatgcgctc ggcatatcgc gcttggcgta cagcgtcagg
    12301 gctggccagc aggtcgccgg tctgcttgtc cttttggtct ttcatatcag tcaccgagaa
    12361 acttgccggg gccgaaaggc ttgtcttcgc ggaacaagga caaggtgcag ccgtcaaggt
    12421 taaggctggc catatcagcg actgaaaagc ggccagcctc ggccttgttt gacgtataac
    12481 caaagccacc gggcaaccaa tagcccttgt cacttttgat caggtagacc gaccctgaag
    12541 cgcttttttc gtattccata aaaccccctt ctgtgcgtga gtactcatag tataacaggc
    12601 gtgagtacca acgcaagcac tacatgctga aatctggccc gcccctgtcc atgcctcgct
    12661 ggcggggtgc cggtgcccgt gccagctcgg cccgcgcaag ctggacgctg ggcagaccca
    12721 tgaccttgct gacggtgcgc tcgatgtaat ccgcttcgtg gccgggcttg cgctctgcca
    12781 gcgctgggct ggcctcggcc atggccttgc cgatttcctc ggcactgcgg ccccggctgg
    12841 ccagcttctg cgcggcgata aagtcgcact tgctgaggtc atcaccgaag cgcttgacca
    12901 gcccggccat ctcgctgcgg tactcgtcca gcgccgtgcg ccggtggcgg ctaa
//
