LOCUS       CP090274                1906 bp    DNA     circular BCT 10-JAN-2022
DEFINITION  Escherichia coli strain E-T84-1 plasmid punnamed5, complete
            sequence.
ACCESSION   CP090274
VERSION     CP090274.1
DBLINK      BioProject: PRJNA792053
            BioSample: SAMN24394415
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Chen,J., Liu,C., Zeng,Y. and Zhang,R.
  TITLE     Phenotypic and Genetic Characterization of Carbapenem-Resistant
            Escherichia coli from ICU Patients in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Chen,J., Liu,C., Zeng,Y. and Zhang,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2022) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine, 88
            Jiefang Rd, Hangzhou, Zhejiang Province 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq 6000; Nanopore PromethION
            48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2022 13:44:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,216
            CDSs (total)                      :: 5,092
            Genes (coding)                    :: 4,918
            CDSs (with protein)               :: 4,918
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 61 of 174
            Pseudo Genes (incomplete)         :: 111 of 174
            Pseudo Genes (internal stop)      :: 34 of 174
            Pseudo Genes (multiple problems)  :: 29 of 174
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E-T84-1"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="punnamed5"
                     /geo_loc_name="China"
                     /collection_date="2020"
     gene            complement(673..960)
                     /locus_tag="LZT39_24745"
     CDS             complement(673..960)
                     /locus_tag="LZT39_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIM53074.1"
                     /translation="MRIKYESRWRLEELKLEICSSIDELINKYGIKYATGINLYMNCED
                     KEFNLVDVINEDGNKIMFVVSNDEKIQKPKKIAKKKTPAPVIELDKYRKK"
                     /name="hypothetical protein"
     gene            complement(967..1506)
                     /locus_tag="LZT39_24750"
     CDS             complement(967..1506)
                     /locus_tag="LZT39_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="firmicute plasmid replication protein RepL"
                     /protein_id="UIM53073.1"
                     /translation="MLEQQFEDFEQQKRRDHQVQDQSKNAKQKRDRLNPPDWSQVTKAG
                     WNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNNTKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFESSLKYSNHDSEQG"
                     /name="firmicute plasmid replication protein RepL"
     Typing          177..437
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.802"
                     /coverage="100.0"
                     /evalue="3.9e-116"
ORIGIN
        1 atgtgcaaat gtaataccgt cacccgcgac gatattccgt ttgccatctg gtcagggctt
       61 cgccccgaca ccccgcatca gtgcgtacgt gctcaatgtg gtttccggtt ctcttccgct
      121 tcctcgccca ctcgctcact aacgttcggt cgctggggct gcggcgagcg gtgccacctc
      181 tcgcatgttg tcggtgattc cgctgttgtt ctttcgggga taagtcgtca gatagccctt
      241 caaacgcgct cagaagctct cagacgcacg aaacggggtt taaatgcccg tcagggcatg
      301 gaaggcgcga aaaacgctta cagagcgttt caggaggtca ggcgcgccga tacccctagc
      361 caggggcttt caggttgcta ggaacagcgt cagccccctg caaagcctat ttgcacggca
      421 caggcagcta ttcggttggt aggtggtcgt agaccattgg tgttacagcg atgggggtct
      481 ggggttctcc ccagacggtt tccggttgcg ctcaaggcgc tatcggaacc gtggcggctc
      541 ggtcgttctg gagagcgggg aacaaaaaca cgcaagggat tcgaggcggc aaaagagcaa
      601 tgcaggatag acaatcatgg tcagaacgtt aacacgtagc ccctgattgt ccgcctgccg
      661 ctggctcaat ggtcattttt ttcgatattt atctagctca attacaggtg ctggagtttt
      721 tttctttgct attttttttg gcttttgtat tttttcatcg ttgctaacga caaacattat
      781 tttatttcca tcttcattga taacatcaac aagattaaat tctttatctt cacaattcat
      841 ataaagattt attcctgttg catatttaat accatattta ttaattaact catctattga
      901 actgcatatt tctaatttta gttcttctaa tctccaacgt gattcatatt taattctcat
      961 agtgcatcac ccctgctctg agtcatgatt agagtacttt aaggaacttt caaactcttc
     1021 acgcctagaa agcataagcc gcaatttacg atctataacc ccatcttttt gagttaatgt
     1081 ttttgcgtta aatgctgcat attttttagt attgttatat ccacgccata cctgataagg
     1141 atctaatgca taggcataat atatgcctac tggtatttta actattgcgc caatactttc
     1201 taaatcccca atccaatatt ggatcgttct taaactaaca ccgaaataat cagctaagaa
     1261 tttttgatca catacaacag ccccacatga tttatctata ttctgggcaa aaaaagaata
     1321 taaccgaaat gctctaggaa ctttttctga tatagcgtcc aacatattcc aaccagcctt
     1381 agtaacctgc gaccagtctg gaggattcaa ccgatctcgt ttttgcttag cgttcttgga
     1441 ttgatcttgt acctgatgat ctcgccgttt ttgctgttca aagtcttcaa attgctgttc
     1501 caacatactt gcatccttac ctgcaatgat atttcgcacc ttacgaaatt tatatcgtac
     1561 agtacgaaac aacacatgca aacatgtttg cgtatacaca tgcaaataac gtttcgtatg
     1621 atggtgtctt aagtcaatga tttttaaaga aatgtcaatt tttgctctaa agatctatca
     1681 aagcgttttt ttactgctca tgatttaaca ctacctaact gcttttgggg tgtcggggcg
     1741 cagccctgac ccagtcacgt agcgatagcg gagtgtatac tggcttactc ctctgcaagc
     1801 cttaccagct gtgatttttg ccagatttca cgctctcaac gttcaacagc tccatcatgg
     1861 ttttgccgtc cgttcaccac taaaacgcct cagaaatcgg gacagg
//
