LOCUS       CP090271                6647 bp    DNA     circular BCT 10-JAN-2022
DEFINITION  Escherichia coli strain E-T84-1 plasmid punnamed4, complete
            sequence.
ACCESSION   CP090271
VERSION     CP090271.1
DBLINK      BioProject: PRJNA792053
            BioSample: SAMN24394415
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Chen,J., Liu,C., Zeng,Y. and Zhang,R.
  TITLE     Phenotypic and Genetic Characterization of Carbapenem-Resistant
            Escherichia coli from ICU Patients in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Chen,J., Liu,C., Zeng,Y. and Zhang,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2022) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine, 88
            Jiefang Rd, Hangzhou, Zhejiang Province 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq 6000; Nanopore PromethION
            48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2022 13:44:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,216
            CDSs (total)                      :: 5,092
            Genes (coding)                    :: 4,918
            CDSs (with protein)               :: 4,918
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 61 of 174
            Pseudo Genes (incomplete)         :: 111 of 174
            Pseudo Genes (internal stop)      :: 34 of 174
            Pseudo Genes (multiple problems)  :: 29 of 174
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E-T84-1"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="punnamed4"
                     /geo_loc_name="China"
                     /collection_date="2020"
     gene            complement(522..953)
                     /locus_tag="LZT39_00975"
                     /pseudo=""
     CDS             complement(522..953)
                     /locus_tag="LZT39_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1162..1485)
                     /locus_tag="LZT39_00980"
     CDS             complement(1162..1485)
                     /locus_tag="LZT39_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UIM48565.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1549..1740
                     /locus_tag="LZT39_00985"
     CDS             1549..1740
                     /locus_tag="LZT39_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UIM48561.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3066..3293
                     /locus_tag="LZT39_00990"
     CDS             3066..3293
                     /locus_tag="LZT39_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="UIM48563.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(3290..4858)
                     /gene="cea"
                     /locus_tag="LZT39_00995"
     CDS             complement(3290..4858)
                     /gene="cea"
                     /locus_tag="LZT39_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="UIM48562.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            5118..5555
                     /locus_tag="LZT39_01000"
     CDS             5118..5555
                     /locus_tag="LZT39_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="UIM48559.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            5636..6055
                     /locus_tag="LZT39_01005"
     CDS             5636..6055
                     /locus_tag="LZT39_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UIM48560.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(6373..6606)
                     /locus_tag="LZT39_01010"
     CDS             complement(6373..6606)
                     /locus_tag="LZT39_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UIM48564.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1826..1913)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          1924..2482
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..1172)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.744"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6270..6646)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.737"
                     /coverage="99.0"
                     /evalue="3.08e-45"
ORIGIN
        1 agggaatcac ctccgctgat ttcccgtacg gaaggacaag gaatttcctg ttcccgtcct
       61 gcacaaactc cacgccccat gtcttcgcgt tcagtttctg caatgtctct tcctgcttcc
      121 tgatttcttc caggttcgcc tgtatcctcc ctccaagata ccagagcgtc ccgccactcg
      181 cggtaaacag gagaaagact atccccagta acatcatgcc cgtattccct gccagcttta
      241 acacgtccct cctgtgctgc atcatcgcct ctttcacccc ttcccggtgt ttttccagcg
      301 attcctctgt cgaggctgtg aacagggcta tagcgtctct gattttcgtc tcgtttgatg
      361 tcacagcctc gcttacagat tcgccgagcc tcctgaactc gttgttcagc attttctctg
      421 tagattcggc tctctctttc agctttttct cgaactccgc gcccgtctgc aaaagattgc
      481 tcataaaatg ctcctttcag cctgatattc ttcccgccgt tcggatctgc aatgctgata
      541 ctgcttcgcg tcaccctgac cacttccagc cccgcctcag tgagcgcctg aatcacatcc
      601 tgacggcctt ttatctctcc ggcatggtaa agtgcatcta tacctcgcgt gacgccctca
      661 gcaagcgcct gtttcgtttc aggcaggtta tcagggagtg tcagcgtcct gcggttctcc
      721 ggggcgttcg ggtcatgcag cccgtaatgg tgattcacca gcgtctgcca ggcatcaatt
      781 ctaggcctgt ctgcgcggtc gtagtacggc tggaggcgtt ttccggtctg tagctccatg
      841 ttcggaatga caaaattcag ctcaagccgt cccttgtcct ggtgctccac ccacaggatg
      901 ctgtactgat ttttttcgag accgggcatc agtacacgct caaagctcgc catcactttt
      961 tcacgtcctc ccggcggcag ctccttctcc gcgaacgaca gaacaccgga cgtgtatttc
     1021 ttcgcaaatg gcgtggcatc gatgagttcc cggacttctt ccggattacc ctgaagcacc
     1081 gttgcgcctt cgcggttacg ctccctcccc agcaggtaat caaccggacc actgccacca
     1141 ccttttcccc tggcatgaaa tttaactatc atcccgcgcc ccctgttccc tgacagccag
     1201 acgcagccgg cgcagctcat ccccgatggc catcagtgcg gccaccacct gaacccggtc
     1261 accggaagac cactgcccgc tgttcacctt acgggctgtc tgattcaggt tatttccgat
     1321 ggcggccagc tgacgcagta acggcggtgc cagtgtcggc agttttccgg aacgggcaac
     1381 cggctccccc aggcagaccc gccgcatcca taccgccagt tgtttaccct cacagcgttc
     1441 aagtaaccgg gcatgttcat catcagtaac ccgtattgtg agcatcctct cgcgtttcat
     1501 cggtatcatt accccatgaa cagaaatccc ccttacacgg aggcatcagt gactaaacag
     1561 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gctgctggag
     1621 aagctcaacg aactggacgc agatgaacag gccgatattt gtgaatcgct tcacgaccac
     1681 gccgatgagc tttaccgcag ctgcctcgca cgtttcgggg atgacggtga aaacctctga
     1741 cacatgcagc tcccggagac ggtcacagct tgtctgtgag cggatgccgg gagctgacaa
     1801 gcccgtcagg gcgcgtcagc aggttttagc gggtgtcggg gcgcagccct gacccagtca
     1861 cgtagcgata gcggagtgta tactggctta accatgcggc atcagtgcgg attgtatgaa
     1921 aagtacgcca tgccgggtgt gaaatgccgc acagatgcgt aaggagaaaa tgcacgtcca
     1981 ggcgcttttc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag
     2041 cggtactgac tcacacaaaa acggtaacac agttatccac agaatcaggg gataaggccg
     2101 gaaagaacat gtgagcaaaa gaccaggaac aggaagaagg ccacgtagca ggcgtttttc
     2161 cataggctcc gcccccctga cgagcatcac aaaaatagac gctcaagtca gaggtggcga
     2221 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2281 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2341 gcgctttctc atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2401 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2461 cgtcttgagt ccaacccggt aaggcacgcc ttaacgccac tggcagcagc cactggtaac
     2521 cggattagca gagcgatgat ggcacaaacg gtgctacaga gttcttgaag tagtggcccg
     2581 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2641 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgttggt agcggtggtt
     2701 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2761 tcttttctac tgaaccgcga tccccgtcag tttagaagag gaggatggtg cgatggtccc
     2821 tccctgaaca tcacgtatat agttagcctg acatccaaca aggaggttta tcgcgaatat
     2881 tcccacaaaa aatcttttcc tcataactcg atccttataa aatgaaaaga atatatggcg
     2941 aggtttaatt tatgagctta agatactaca taaaaaatat tttatttggc ctgtactgca
     3001 cacttatata tatatacctt ataacaaaaa acagcgaagg gtattatttc cttgtgtcag
     3061 ataagatgct atatgcaata gtgataagca ctattctatg tccatattca aaatatgcta
     3121 ttgaatacat agcttttaac ttcataaaga aagatttttt cgaaagaaga aaaaacctaa
     3181 ataacgcccc cgtagcaaaa ttaaacctat ttatgctata taatctactt tgtttggtcc
     3241 tagcaatccc atttggattg ctaggacttt ttatatcaat aaagaataat taaatcccta
     3301 acacctcatt tatagtatta agtttattct tatcaatata ggagcataga attcctgtaa
     3361 caatagcaat accccaaata cctaatgtag ttccagcaag caagctaaaa agtaaagcaa
     3421 caacataact cacccctgca tctgcagctt tcttctctaa tgtaagaaat agtggcttcc
     3481 agtcacctgt atccttaatt tttaggatat cagataccac atcatatcca aaagaaacat
     3541 gccccgtaat cttcaagtac ttggcaaact gatctaaatg tttagcccag tcatcatact
     3601 tcaccgatgc caacgcatta aaaatagcat cacgatcggc cttgctgaat ttcttattta
     3661 aaacatcctt gtatttttca aaagcagcga gagcttcatt cacattgccg attttcttac
     3721 ctttagactt atcagcaagt tcctgtgcca ttttcgaata tttttcacca tatttttcag
     3781 tcagcgtttg ataaaagcta actgttgcat caacagcatc cttaatctgt gaattaagga
     3841 gattattctg tgcttttttc aaattttctt cagcttcatg aacacgagcg ataccggcat
     3901 tacgattatt actgacctga gaaatagcct tctggatctg agttatatca gcatttatcc
     3961 ggttaatacg tgtttctgat gctgttacct gtttttgttt ttcttctcta atcttaccgg
     4021 ccccaacccg tcgtctggtt gcttcaaaaa aaggacggtt ctgaagcgga tcattggctc
     4081 ttggtgatag ttttttgacc agctcatcca gttctttata tttagcggat gcctgagcca
     4141 gttcatttcg ttttccagcg agcgttttca tttctgcatc acgggcatgg atactggagc
     4201 ttaaacgaga attgagagtc ttaatctctc catccatttt caccacttca gattgtgcag
     4261 cagaaagttt tttttgggcg atctcaacag ctttagcttc ttcactcaat gcagccagtc
     4321 gtttctcttc agcttcagcc agtttcaact ggcgttctgt ttcagccttc tcccgttcaa
     4381 tctctttacg tcgttgttct gcttcctgaa aagccttttc tgctgcttcc gcttctttac
     4441 gggctttttc ttctgctttc gcaaggcgca aacgctctgc ttccgcctgc atagctgcat
     4501 tattagcatg agcaagctct gttgctgaag gcgtacgtga ggcattgtga cgaagagcct
     4561 cattcacgat atccttcagg cgctgagtca gcgcatccct gtttgccttt gctttcgcct
     4621 gtgcttccgc tgcagctttt gcccgggcag cctgctctgc ctgtgttttc tttaattgag
     4681 cagtagacca tttagcagtt gcatgaatag ctgcagaact ttcactttta ctgcctcctt
     4741 ttccacctcc gccgccagag ccactcccgt caggagtacc attcaaaaga gtaataatta
     4801 cctgtccctt atcatcataa ggaacaccat ctttatagta cgctaccgcg gtttccatta
     4861 taaaatcctc tttgactttt aaaacaataa gttaaaaata aatactgtac atataaccac
     4921 tggttttata tacagcataa aagctacgcc gctgcatttt ccctgtcaag actgtggact
     4981 tccatttttg tgaaaacgat caaaaaaaca gtctttcaca ccacgcgcta ttctcgcccg
     5041 atgccacaaa aaccagcaca aacattaccg ttctcagacc tcattatgtt ttactgaaac
     5101 tatgagatga gacatctatg ggacactgtc actttatggc atggcacaca ctccgggacg
     5161 cactaaaaat gacaggcaga tcgcgttcac agttttaccg tgatatgcgc gcaggccttg
     5221 tcagttaccg taccggcagg gacggacgac gggagtttga aaccagtgaa ctgatccggg
     5281 catacggcga attaaagcag aatgagacac cagaaaggca cagtgaggga catgcagaaa
     5341 atccacatga tcagcagaca gaacgcattc tccgggagct gaatgagctg aaacaatgcc
     5401 tgacgctgat gcttgaggat aaacaggcac aggatatgga tcgcagacgc caggaagcag
     5461 aacgggaaca gctacaaaat gagatagccc agctcaggca ggcactggaa ctggaaaaga
     5521 aacggggatt ctggtccagg ttgttcggtc gctgaacgct gtcagagact gatgataaaa
     5581 tagtcttcgg ataataactc accgagaata aatactttaa ggtagggaga cactcatgag
     5641 acgtaccgga aacaaacttt gtcttatcgc catgataaca gcaacagtag ctctcacagc
     5701 ctgtacccca aagggcagcg tggaacaaca tacccggcat tacgtatatg cttctgatga
     5761 cggttttgat cccaactttt ccacccaaaa agccgacaca acacgaatga tggtgccttt
     5821 ttttcggcag ttctgggata tgggagctaa agataaagcg acaggaaaat cacggagtga
     5881 tgtgcaacaa cgcattcagc agtttcacag ccaggaattt ttaaactcac tccggggcac
     5941 aactcaattt gcgggtactg attaccgcag caaagacctt accccgaaaa aatccaggct
     6001 gctggctgac acgatttctg cggtttatct cgatggctac gagggcagac agtaagtgga
     6061 tttaccataa tcccttaatt gtacgcaccg ctaaaacgcg ttcagcgcga tcacggcagc
     6121 agacaggtaa aaatggcaac aaaccacccg aaaaactgcc gcgatcgcgc ctgataaatt
     6181 ttaaccgtat gaatacctat gcaaccagag ggtacaggcc acattacccc cacttaatcc
     6241 actgaagctg ccatttttca tggtttcacc atcccagcga agggccatcc agcgtgcgtt
     6301 cctgtatttc cggctgacgc tcccgttcca gggataacac atgttcgcgc tcctgtatca
     6361 gccgttcctc tcttatctcc agttctcgct gtataactgg ctcaagcgtt ctgtctgctc
     6421 gctcaagtgc tgcacctgct gactcaactg catgacccgc tcgttcagca tcgcgttgtc
     6481 ccgttgcgta agcgaaaaca tcttctgcaa ttccacgaag gcgctctccc attcgctcag
     6541 ccgctgcata tagtcctgtt gcagctgctc taaggcgttc agcaaatgtg tttccagctc
     6601 tgtcactctg tgtcactcct tcagatgtac ccactctttc ccctgaa
//
