LOCUS       CP090270                8136 bp    DNA     circular BCT 10-JAN-2022
DEFINITION  Escherichia coli strain E-T84-1 plasmid punnamed3, complete
            sequence.
ACCESSION   CP090270
VERSION     CP090270.1
DBLINK      BioProject: PRJNA792053
            BioSample: SAMN24394415
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Chen,J., Liu,C., Zeng,Y. and Zhang,R.
  TITLE     Phenotypic and Genetic Characterization of Carbapenem-Resistant
            Escherichia coli from ICU Patients in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Chen,J., Liu,C., Zeng,Y. and Zhang,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2022) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine, 88
            Jiefang Rd, Hangzhou, Zhejiang Province 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq 6000; Nanopore PromethION
            48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2022 13:44:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,216
            CDSs (total)                      :: 5,092
            Genes (coding)                    :: 4,918
            CDSs (with protein)               :: 4,918
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 61 of 174
            Pseudo Genes (incomplete)         :: 111 of 174
            Pseudo Genes (internal stop)      :: 34 of 174
            Pseudo Genes (multiple problems)  :: 29 of 174
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E-T84-1"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="punnamed3"
                     /geo_loc_name="China"
                     /collection_date="2020"
     gene            join(7369..8136,1..171)
                     /locus_tag="LZT39_00940"
     CDS             join(7369..8136,1..171)
                     /locus_tag="LZT39_00940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="UIM48555.1"
                     /translation="MRYISGLKTLAKDFYHYNFSGMNELFSYDDTHEDNIEIFMKDNSF
                     LEIPKVIWMYWDDASIPPYISLMIDKIRNDNKDHDLVVLNKNNLVSYIEDLKFSSDKYI
                     PIANKSDIIRLELLKRYGGIWIDSSVILTKPISWVHDINKDKKVDLIGYYRDVSTKDRM
                     YPIVESWFLCSQKNNLFINEWLDVLSPLKEMGAKEYFNMIKSRPDYEVILQGIHKPDYL
                     LVYLAQQIAMRKINFNLFSKRSESSAFLYQENESWNSSAIARDLCIKKSPITLPPVIKL
                     TNDNRRGIEKLIKLKLVKKDSIIGKLMSGAL"
                     /name="capsular polysaccharide synthesis protein"
     gene            236..933
                     /locus_tag="LZT39_00945"
     CDS             join(236..485,485..933)
                     /locus_tag="LZT39_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="UIM48556.1"
                     /translation="MASVSISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVNSRIQPGSNVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVVHVFGERTMATLERLPGLLSAFEVVVW
                     MRDGWPLYEPRLKGELHVISKRYTQRIERHNLNLRQHLSRLGRKSLSFSKSVELHDKVI
                     GHYLNIKHDL"
                     /name="IS1 family transposase"
     gene            1667..1898
                     /locus_tag="LZT39_00950"
                     /pseudo=""
     CDS             1667..1898
                     /locus_tag="LZT39_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332449.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            2457..3680
                     /locus_tag="LZT39_00955"
     CDS             2457..3680
                     /locus_tag="LZT39_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="UIM48557.1"
                     /translation="MMAEKLVSIIGLVFVNSYMAKYIGPTDFGKITFATTIFTFVQAMA
                     WFGGQNILFKRMSEKRKSGIALALHTQKQRRVIFALSSLIALFYLFFFTDTLTFVFGVA
                     NCIATYYIVMDIFAVYNNTQLISRINALTNGAGLCIAYIFRFLISYLELPVYTFSIPII
                     LIPAIPYVIRCIYFYNTVEDTSVLKSKTKKYNKYLISAGGSLLLSTLSIVVYTQVSNIF
                     LAKVTSYSNLGIYNVALTLGSAYSFISVALITSFFSKIYQEKDFEKMSRLLTSINRIVI
                     VITIFILFGFYFLGPYIISMLYGSAYKMAVELVPIIILATMFSSLGAICYRFIIKYSGY
                     KYLSYKMMLTGLLSIPFSYVMINRYDVFGAAYCFLVVEVLSCTLLNYFFRSGMILRIHL
                     NVIGLMRR"
                     /name="oligosaccharide flippase family protein"
     gene            3773..4470
                     /locus_tag="LZT39_00960"
     CDS             join(3773..4022,4022..4470)
                     /locus_tag="LZT39_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="UIM48558.1"
                     /translation="MASVSISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVNSRIQPGSNVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVVHVFGERTMATLERLPGLLSAFEVVVW
                     MRDGWPLYEPRLKGELHVISKRYTQRIERHNLNLRQHLSRLGRKSLSFSKSVELHDKVI
                     GHYLNIKHDL"
                     /name="IS1 family transposase"
     gene            5393..5584
                     /locus_tag="LZT39_00965"
     CDS             5393..5584
                     /locus_tag="LZT39_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UIM48554.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6441..7356
                     /locus_tag="LZT39_00970"
                     /pseudo=""
     CDS             6441..7356
                     /locus_tag="LZT39_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004088970.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(1328..1809)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.775"
                     /coverage="93.0"
                     /evalue="4.5400000000000006e-173"
     Typing          complement(4631..5156)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.617"
                     /coverage="99.0"
                     /evalue="2.78e-170"
ORIGIN
        1 tggaactctt cagctatagc aagggatctt tgcattaaaa aatctccgat aacattacca
       61 ccagtaataa aactaaccaa tgataatagg cgcggaattg aaaagctcat aaaattaaaa
      121 ttagtaaaga aagatagcat cattggaaaa ttaatgtcag gagcactcta acgagaaggc
      181 ggtaatggtg ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc
      241 ttctgtttct atcagttgtc cctcctgttc agctactgaa ggcgtggtgc gtaacggtaa
      301 aagtactgcc ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcagctaca
      361 gttcacttac accgcctctc agcccggtac acaccagaaa attattgata tggccatgaa
      421 tggcgtcgga tgtcgcgcca gtgcacgcat tatgggcgtt ggcctcaaca cgattttacg
      481 acacttaaaa aactcaggcc gcagtcggta aactcacgca tacaaccggg cagtaacgtc
      541 attgtttgcg cggaaatgga cgaacagtgg ggttacgtcg gcgctaaatc acgccagcgc
      601 tggttgtttt acgcgtatga caggatacgg aggacagttg tggtgcacgt attcggtgaa
      661 cgcacgatgg ccacgctgga gcgtcttccg ggcctgctgt cggcctttga ggtcgtggta
      721 tggatgaggg atggctggcc gctttatgaa ccacgcctga agggagaact gcacgttatc
      781 agcaagcgat atacgcagcg tattgagcgg cataacctga atctgaggca gcatctgtca
      841 aggctgggca ggaagtcact gtcgttctca aaatcggtgg agctgcatga caaagtcatc
      901 gggcattatc tgaacataaa acacgatctg taagttggag tcattaccta cagtttcata
      961 tgtgggcgtt tatgtaacat atgtcgctgg gccaactctt ctggtttctg ggtttttatc
     1021 gtggattaca aggccgtaat ctgataatgc ccctaatcgg tcagatctct aggatagtag
     1081 caatacagca aggaacttct tgagatcctt tctgttgcgt cgtaatctca tgtcctgcaa
     1141 acaaaaaaac caccgctacc aacggtggtt cgtttgccgg aatcaagagc taccaactct
     1201 tttttccgaa ggtaactggc tttcacggag cgcagatacc aaaacagtcc ttccagtgta
     1261 gccgcagtca ggccaccact tcaagaactc tgtagaagca ctctactact ctgctccgta
     1321 aatcctgtta ccagtggctg ctgccagtgg cgttttgtcg tgcctctccg ggttggactc
     1381 aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca
     1441 gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagataaac
     1501 gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga
     1561 gggagccatc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact
     1621 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggca
     1681 acgccgcatc ctttctgaga cctctttatt cctgtgtcat agctttatgc tcttatccgt
     1741 gagcttcgtc cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1801 cggaatatca cctgaaccag catcttctcc tgacgccgga aagcctcatt tcactcctgc
     1861 cacatgaagc actttggcaa atcaccactt cggtttaaaa tattaagaaa acgttacgcc
     1921 atcataagca agggtgaaat cggtccttcc tgaagttatc gtgtttattg ttaagttact
     1981 taatgatttg acctcaaccc aaatgttaaa gatagtcgtt agcgttagtg cagtggatag
     2041 gtagctgaag agcccagggc ggtagcgtgg ctaagttgtt gtttttatgg gtttttattc
     2101 aattcataag atgcagtaga tatgtcaaaa gagagctttt aggtggattc tttacagtag
     2161 ttagaccacg acaaaaatca gcttttgaat cgccacggat aatctagaca cttccgagac
     2221 gttgataata ctggttttca tattctgtcg gtgacatctg atcgctggaa ccatgccgac
     2281 gctggtaatg ataccaaatt gttgatttcg tgttttcact gacaccagga ttatcctgat
     2341 gttacaagga ttgaatgact acagattaaa atagtcatca acaggttgac agtattacca
     2401 aaatttctat gggattaatt ttttgcgagt gagtaatgta ttctaatgcc ttttggatga
     2461 tggctgaaaa attagtcagc atcatcggtc ttgtgtttgt aaactcctat atggcaaaat
     2521 atataggacc tactgatttt ggtaagatta cttttgctac gacaatattt acgtttgtac
     2581 aggccatggc ttggtttggt gggcaaaaca tcctttttaa aaggatgagt gagaaaagaa
     2641 aatcaggtat tgctcttgca ttacatactc aaaagcagcg cagggttatt tttgcactct
     2701 cttcattgat tgccttgttc tatttgtttt tttttacaga caccctgaca tttgtttttg
     2761 gggtggctaa ctgcattgcc acttattata tagtgatgga tattttcgct gtatataata
     2821 atacgcagtt aatatctaga attaatgcat taacaaatgg cgcagggttg tgtattgctt
     2881 atatattccg ctttttaatt tcatacttgg agttgccagt atatacattt agcattccta
     2941 ttatattaat tccagcaata ccttatgtta ttaggtgtat atatttttac aataccgttg
     3001 aagacacttc ggtattaaaa tcgaaaacga agaaatataa taagtattta atttcagcgg
     3061 gagggtcgtt gttactatca accctgtcca ttgtggttta cacacaggta tcaaatattt
     3121 ttttggctaa agtaacctca tattctaatt tgggtattta taatgtggcg ctgactcttg
     3181 gcagtgcgta ttcatttatt tcagttgcgt taattactag tttcttttct aaaatctatc
     3241 aagagaaaga ttttgaaaaa atgtctagac ttctaacctc aataaataga attgtgatcg
     3301 tgataacaat ttttatctta tttggttttt attttcttgg gccgtacatt atcagtatgc
     3361 tctatggttc ggcctataaa atggctgttg agttggtgcc aatcattatt ctcgcgacaa
     3421 tgttttcatc cttgggagct atttgttata gatttataat aaaatactct ggctacaaat
     3481 acctttcata taaaatgatg ttaactggct tgctcagcat cccattctca tatgttatga
     3541 ttaaccgtta tgatgttttc ggtgctgcgt attgttttct tgtggtcgag gttctgtcat
     3601 gcacattatt aaattatttc tttaggagtg gaatgattct aaggattcat ttaaatgtta
     3661 ttgggttgat gaggcgttga tggtgggggg ggggcaggtt tatttatagg ttgtgttggt
     3721 aatggtgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc
     3781 tgtttctatc agttgtccct cctgttcagc tactgaaggc gtggtgcgta acggtaaaag
     3841 tactgccgga catcagcgct atctctgctc tcactgccgt aaaacatggc agctacagtt
     3901 cacttacacc gcctctcagc ccggtacaca ccagaaaatt attgatatgg ccatgaatgg
     3961 cgtcggatgt cgcgccagtg cacgcattat gggcgttggc ctcaacacga ttttacgaca
     4021 cttaaaaaac tcaggccgca gtcggtaaac tcacgcatac aaccgggcag taacgtcatt
     4081 gtttgcgcgg aaatggacga acagtggggt tacgtcggcg ctaaatcacg ccagcgctgg
     4141 ttgttttacg cgtatgacag gatacggagg acagttgtgg tgcacgtatt cggtgaacgc
     4201 acgatggcca cgctggagcg tcttccgggc ctgctgtcgg cctttgaggt cgtggtatgg
     4261 atgagggatg gctggccgct ttatgaacca cgcctgaagg gagaactgca cgttatcagc
     4321 aagcgatata cgcagcgtat tgagcggcat aacctgaatc tgaggcagca tctgtcaagg
     4381 ctgggcagga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg
     4441 cattatctga acataaaaca cgatctgtaa gttggagtca ttacctacag tttcatatgt
     4501 gggcgtttat gtaacatatg tcgctgggcc aactcttctg gtttctgggt ttttatcgtg
     4561 gattacaagg ccgtaatctg ataatgcccc taatcggtca gatctctagg atagtagcaa
     4621 tacagcaagg aacttcttga gatcctttct gttgcgtcgt aatctcatgt cctgcaaaca
     4681 aaaaaaccac cgctaccaac ggtggttcgt ttgccggaat caagagctac caactctttt
     4741 ttccgaaggt aactggcttt cacggagcgc agataccaaa acagtccttc cagtgtagcc
     4801 gcagtcaggc caccacttca agaactctgt agaagcactc tactactctg ctccgtaaat
     4861 cctgttacca gtggctgctg ccagtggcgt tttgtcgtgc ctctccgggt tggactcaag
     4921 acgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc
     4981 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gataaacgcg
     5041 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
     5101 agccatcagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat
     5161 ttgagcgtca gattttgtga tgcttgtcag gggggcggag cctatggaaa aacgccccgg
     5221 cggggcgtgg ccttttcgcc cttgtcctta gctttcgtta tgcttaccgt taggttatct
     5281 gcttatcttc cctccgctcg ccgcagactc atgaccgaac ggagtgagcg aatgagcgag
     5341 gaagcggaaa acaatcagaa cgtacagtaa gcactttcgc gccggggcct tcatgaacaa
     5401 acaacaaaag acagccgtta acatggctaa attcatccag gatcagtccc tgcttcttct
     5461 ggacaggctt aatgagctgg atctggatca tgaggctgat ttctgcgaga aactccatga
     5521 ggatgcggaa cgcctttacc gaagcctgtc agcgcgcctg aatgagcagc aggacggtgc
     5581 gtaatcgctc cgttgtgggt attaccggag taataccggc atcgccttaa ttttcattgt
     5641 cttggcgctt aatattatta ccccggtaat acccagaatc cctgtatttt ttctttttta
     5701 tttatttttc atgaagttgc gatgtacatc gcagctgcgt taggtgtatg actatttaag
     5761 ggttaaaaaa tcatcatttt tggtagggcg ctctccgtag gttaagggtt acttggctaa
     5821 aaaacgtcct gttctttgat ggtcatgctt gcatgaccat ctgagcaacc aaaaactaca
     5881 gataaactac aaagtaacta ccggattacc tcagatccgt tccctgaacc attcgtacat
     5941 ccgccccctc ttcctgggcc tcctgcacgt gctgaaaatg gtttttccgg ctgtgccggc
     6001 aacgcctcac ggccacgcaa taaattgcgt ccccgttcac cgttttcgga tgggaagaac
     6061 gccggaaaca ggacttaccc cggctgaatg gcgtgaattt ccgcgctatg cacttgcgcg
     6121 catactcatg catgccgtaa aaacagagcc tgcgcgtttc tggcgggttt tcgggtggtt
     6181 tgttgcctgt tttgccggtt tcccgtcaga aacgcgctga gggcgcctga ggcggtgcgg
     6241 gcaacggatg ttatggtaaa tctcggcggg atcgacagat gtggtcagag ggtgtaactt
     6301 taaggtcaga aaaaacaaag ccccggaaat catgtgctat cttggcggat ctcatgataa
     6361 ccagggccag gtcgaaacct tgaaaggata ttagcacatg aaccgtgcca gacaacagcc
     6421 cgccataagc gcagggcaca ttgcgtaacg ccgcactcgg actttttaac gaccgcttac
     6481 cgcacaagcc gtacttatcg gacgatctgc acttcggtgt acgcattgca gggaaagagc
     6541 gcgctatcct ggcgaaatac atccagttca accagcctta cgccatgttc tggctgggat
     6601 ttgacgtcga tcgcgtcggc gcggctatcg actggagcga ccgtaacgcc cccgcaccta
     6661 cgttgaccat taccaatccc gaaaatggtc acgcgcacct gctgtacgcg ctggaaacct
     6721 cgatccgcac cgccccggac ggaaaaatga agccgctgaa gtacgctgct gtcgtagaaa
     6781 acgcgctgcg taaaaaatta ggcgctgaca caggctattc tggcctgatt tgcaagaacc
     6841 cgaaccacag tcactggaaa atcgccgtgt ggcagccgca gctttacacg ctggactggt
     6901 tagccgactt actcgacctg accgccgcga acgacaaaga aatcgttgct gactacggat
     6961 tagtccgtaa ctgcaccctg tttgataaaa cccgtaagtg ggcatatcgc gctatccgcc
     7021 agggctggcc tgaatatgac cagtggctgc aagcctgcta tgaacgtgcc agtgcctata
     7081 atctgcaatt ttcagctcct ctggatgaaa atgaggtgaa tgggatcgct aaaagcattg
     7141 ctaagtggac tcacgggaaa tttacggcag aatcatttta tgaatttgta aaaagtacac
     7201 atatgtcaaa aattcaatct gaaagaggaa ggaagggggg ggattggtgg tgtaataagc
     7261 cgtggaggag ggaggcctaa aagaaaaagt agtccatgga ttgatttggg tattagtcgt
     7321 agcacttggt atcgaaagta ttttaaaatt aattgaaata gggtgtgtat gagatacatt
     7381 agtgggttaa aaacacttgc aaaagatttt tatcattata atttttccgg aatgaatgag
     7441 ttgtttagct atgatgatac gcatgaagat aacatagaaa tatttatgaa agataattct
     7501 tttctggaaa taccgaaggt aatctggatg tactgggatg acgctagtat tccgccttac
     7561 ataagtctga tgatagataa aataaggaat gacaacaaag atcatgattt agttgttttg
     7621 aataaaaata atttagtcag ttacattgaa gatttgaaat tttcttctga taaatacatt
     7681 cctatagcaa acaagtcaga cataataaga cttgaattat taaagcgtta cggaggaata
     7741 tggattgact cttctgttat cctgacaaag cccatatcat gggttcatga tattaataaa
     7801 gataaaaaag tcgatcttat tggttattat agggatgtct caacgaaaga tagaatgtat
     7861 cctattgttg agagctggtt tttatgctca cagaaaaata atttgttcat taatgaatgg
     7921 cttgatgttc tttcaccatt aaaagaaatg ggtgcaaagg aatactttaa tatgataaaa
     7981 tccaggcccg actatgaagt tattctccaa ggcattcaca agcctgacta tttacttgtt
     8041 tacctcgcac agcaaattgc aatgagaaaa attaacttta atttattttc taaaagaagc
     8101 gagtccagtg cattcttata tcaagagaat gagagt
//
